FastQCFastQC Report
Sat 18 Jun 2016
SRR3550104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997001
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT69230.6943824529764765TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC58950.5912732284120076TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC49260.49408175117176417TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC21820.2188563501942325TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCC18920.1897691175836333No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18080.1813438502067701No Hit
ACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC17150.17201587561095727TruSeq Adapter, Index 22 (95% over 21bp)
TGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCTT13120.13159465236243495TruSeq Adapter, Index 20 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG12710.12748231947610886No Hit
GCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG11180.11213629675396514TruSeq Adapter, Index 20 (95% over 21bp)
AAAACTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC10910.10942817509711625No Hit
GGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG10070.10100290772025304TruSeq Adapter, Index 20 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACGTA207.032835E-445.00000434
ATATGCG406.8139343E-945.0000041
CATAGGT207.032835E-445.00000419
CTAAACG207.032835E-445.0000041
AACGCAC750.045.00000431
TGCCTCG207.032835E-445.00000429
GTTCACG207.032835E-445.0000041
AACCTCG207.032835E-445.00000417
ACCGTGC207.032835E-445.00000424
GCGCTAC207.032835E-445.00000437
CCGTTAT207.032835E-445.00000418
GTACGAC207.032835E-445.00000416
TCGCAAT253.8904334E-545.021
GTCGATG351.2117198E-745.01
CGAAACG253.8904334E-545.02
GTTTACG253.8904334E-545.01
CGCATAC253.8904334E-545.037
ATTACGC253.8904334E-545.012
TAACGTG253.8904334E-545.042
GTCCGTT253.8904334E-545.041
Produced by FastQC (version 0.11.5)