FastQCFastQC Report
Sat 18 Jun 2016
SRR3550102_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550102_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1185835
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC37080.3126910573562089No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT35320.29784919487112455No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC31920.26917741507039344No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG16170.13635961158171248No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA14720.12413194078434184No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC12240.10321840728263207No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAT302.1655596E-645.0000043
GGTACGA302.1655596E-645.0000048
CTCGTAG453.8562575E-1045.0000041
TATTGCG302.1655596E-645.0000041
GAACGTA302.1655596E-645.0000042
ACGTATC302.1655596E-645.00000411
CCGAACG302.1655596E-645.0000041
CACGTCG302.1655596E-645.00000418
AGCGTAC351.2119744E-745.032
CAAACGT207.0333766E-445.036
CGTATCA207.0333766E-445.039
CGAGTTA207.0333766E-445.026
GAGTCAT253.890881E-545.07
GCGCGAA406.8157533E-945.020
ATTCGTT351.2119744E-745.011
CGTTCGA253.890881E-545.040
CCCCGTA207.0333766E-445.036
TCAATCG253.890881E-545.018
TCGGACA207.0333766E-445.035
TAACACG207.0333766E-445.01