##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550102_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1185835
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.119912972715426	33.0	31.0	34.0	30.0	34.0
2	32.427517319019934	34.0	31.0	34.0	30.0	34.0
3	32.488008871385986	34.0	31.0	34.0	31.0	34.0
4	35.9881484354906	37.0	35.0	37.0	35.0	37.0
5	30.468109812916637	37.0	35.0	37.0	0.0	37.0
6	32.94846500567111	37.0	35.0	37.0	17.0	37.0
7	35.09334856873005	37.0	35.0	37.0	32.0	37.0
8	35.616988872819576	37.0	35.0	37.0	35.0	37.0
9	37.4855262325703	39.0	37.0	39.0	35.0	39.0
10	37.46816546990095	39.0	37.0	39.0	35.0	39.0
11	37.380181897144205	39.0	37.0	39.0	35.0	39.0
12	37.23167641366632	39.0	37.0	39.0	34.0	39.0
13	37.182378661449526	39.0	37.0	39.0	34.0	39.0
14	38.42470748459946	40.0	38.0	41.0	34.0	41.0
15	38.54089987224192	40.0	38.0	41.0	35.0	41.0
16	38.613041443371124	40.0	38.0	41.0	35.0	41.0
17	38.6116449590373	40.0	38.0	41.0	35.0	41.0
18	38.55123773543537	40.0	38.0	41.0	35.0	41.0
19	38.53280009444821	40.0	38.0	41.0	34.0	41.0
20	38.46104980878453	40.0	37.0	41.0	34.0	41.0
21	38.41780433196861	40.0	37.0	41.0	34.0	41.0
22	38.39275868902503	40.0	37.0	41.0	34.0	41.0
23	38.339021027377335	40.0	37.0	41.0	34.0	41.0
24	38.33477929054211	40.0	37.0	41.0	34.0	41.0
25	38.253110255642646	40.0	37.0	41.0	34.0	41.0
26	38.15773358013551	40.0	37.0	41.0	34.0	41.0
27	38.11345254609621	40.0	37.0	41.0	34.0	41.0
28	38.03912095696281	40.0	37.0	41.0	34.0	41.0
29	38.00672859208912	40.0	36.0	41.0	34.0	41.0
30	37.922000952915035	40.0	36.0	41.0	34.0	41.0
31	33.3113603494584	39.0	34.0	41.0	9.0	41.0
32	34.200784257506314	39.0	33.0	41.0	16.0	41.0
33	36.15434862354375	39.0	34.0	41.0	30.0	41.0
34	36.97976868620002	39.0	35.0	41.0	31.0	41.0
35	37.130167350432394	39.0	35.0	41.0	32.0	41.0
36	37.28748181661024	39.0	35.0	41.0	33.0	41.0
37	37.351004144758754	39.0	35.0	41.0	33.0	41.0
38	37.32417494845404	39.0	35.0	41.0	33.0	41.0
39	37.3498690795937	39.0	35.0	41.0	33.0	41.0
40	37.29854406388747	39.0	35.0	41.0	33.0	41.0
41	37.247239287084625	39.0	35.0	41.0	33.0	41.0
42	37.126908886986804	39.0	35.0	41.0	33.0	41.0
43	37.12023342201908	39.0	35.0	41.0	33.0	41.0
44	37.0327895533527	39.0	35.0	41.0	32.0	41.0
45	37.02300657342716	39.0	35.0	41.0	32.0	41.0
46	36.99458525005587	39.0	35.0	41.0	32.0	41.0
47	36.91369962937508	39.0	35.0	41.0	32.0	41.0
48	36.885910771734686	39.0	35.0	41.0	32.0	41.0
49	36.84083451744973	39.0	35.0	41.0	32.0	41.0
50	36.65041257847846	39.0	35.0	41.0	32.0	41.0
51	36.05492585393415	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	11.0
11	13.0
12	10.0
13	33.0
14	25.0
15	34.0
16	49.0
17	97.0
18	222.0
19	375.0
20	671.0
21	1063.0
22	1771.0
23	2802.0
24	3932.0
25	5208.0
26	6201.0
27	7700.0
28	9500.0
29	12042.0
30	16080.0
31	22352.0
32	35712.0
33	41990.0
34	75169.0
35	115958.0
36	136435.0
37	142060.0
38	210231.0
39	337628.0
40	447.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.04156986427286	21.50594306965134	24.411827952455443	11.040659113620361
2	30.809007998583276	26.44027204459305	24.50889035995733	18.241829596866342
3	31.418957949461774	25.287244852783058	25.86649913352195	17.42729806423322
4	29.693591435570717	26.072345646738377	24.7464444884828	19.487618429208112
5	22.590663962524296	40.16747692554192	20.34920541222008	16.8926536997137
6	26.784249073437707	35.37853073994274	21.878254563240247	15.958965623379306
7	79.67541858690291	7.474311350230006	7.691879561659085	5.158390501208009
8	80.02293742384059	6.642829736008804	7.673495891080968	5.660736949069643
9	74.20728853508287	8.396277728351752	10.272255414960767	7.1241783216046075
10	39.651890861713476	28.310178060185436	16.59885228552033	15.439078792580755
11	30.69499550949331	27.078640788979918	22.615793934232	19.610569767294773
12	28.870121053940895	23.75971361951705	27.842322076848802	19.527843249693255
13	24.95667609743345	26.325416267861883	28.69783738884415	20.02007024586051
14	19.489136346962265	29.931988851737383	28.025146837460525	22.553727963839826
15	18.613803775398768	27.821577200875332	33.39823837211754	20.16638065160836
16	20.244047443362696	26.561705464925563	30.941741473307836	22.25250561840391
17	20.96851585591587	26.89910485016887	26.4098293607458	25.722549933169454
18	21.733040431425955	26.377362786559683	29.197232329961587	22.692364452052775
19	23.914372572912757	27.54700274490127	26.215198573157313	22.32342610902866
20	25.903603789734657	26.89986380904595	27.172330045917015	20.024202355302382
21	24.172249933591097	27.057558597949967	27.19914659290711	21.571044875551827
22	21.813237086103886	27.079905720441715	25.743632124199404	25.363225069254998
23	21.350609486142677	28.042181247812724	27.340734587864247	23.266474678180355
24	22.53576593708231	25.226190827560323	28.812608836811194	23.425434398546173
25	20.921460405537026	29.11399983977535	26.129773535103958	23.83476621958367
26	21.093491084341416	29.8332398689531	26.74571082823496	22.32755821847053
27	23.22203341948922	29.431075992865786	25.649183908385233	21.697706679259763
28	19.476402703580177	29.568784864673415	27.72316553314753	23.23164689859888
29	20.846492134234527	29.641391930580564	26.357123883170928	23.15499205201398
30	24.054105335059262	28.30267280017878	24.674174737632132	22.96904712712983
31	24.989648644204294	30.68782756454313	22.245337673453726	22.07718611779885
32	23.13981287447242	32.68641927418233	22.712181711620925	21.46158613972433
33	26.030855894791433	29.364118954154666	23.0217526047047	21.583272546349196
34	21.560756766329213	29.498454675397507	24.995467328928562	23.945321229344724
35	23.26765528087803	28.498315532936708	24.900428811765547	23.333600374419714
36	25.659387688843726	29.81426589702615	23.054219178890822	21.472127235239306
37	23.144872600319605	31.153912643833248	24.706219667997654	20.994995087849492
38	22.63046713918884	30.805297532962005	24.164913331112675	22.399321996736475
39	24.704954736535857	26.696547158753113	25.388945342311537	23.20955276239949
40	24.106473497577657	26.484881960812423	27.147706046794028	22.26093849481589
41	21.49970274110648	28.045385740849277	25.351334713514106	25.103576804530142
42	23.186109365974186	28.48431695809282	25.392655807932808	22.936917868000187
43	24.789536486948016	26.550911383118226	26.63574611982274	22.02380601011102
44	22.023890338875137	28.055842507600133	25.651966757601187	24.268300395923546
45	22.661921768205527	26.73980781474657	24.76314158377852	25.835128833269383
46	22.834121104538152	27.99639072889567	25.673554921215853	23.495933245350322
47	22.15611784101498	26.625795325656604	28.3100094026572	22.908077430671213
48	21.301024172840236	26.234088216320146	28.1183301218129	24.34655748902672
49	22.12828934885545	25.73865672711634	28.596980186956873	23.536073737071348
50	20.938832130945706	26.351473855974906	27.57626482605084	25.13342918702855
51	21.347910965690843	25.140428474450495	25.39746254748764	28.114198012371027
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	923.0
1	1373.0
2	1823.0
3	2197.0
4	2571.0
5	2185.0
6	1799.0
7	1800.5
8	1802.0
9	1862.5
10	1923.0
11	2043.0
12	2163.0
13	2394.0
14	2625.0
15	2704.0
16	2783.0
17	2800.0
18	2817.0
19	2919.5
20	3022.0
21	3331.0
22	3640.0
23	3983.0
24	4326.0
25	5974.0
26	9510.5
27	11399.0
28	15048.5
29	18698.0
30	19805.0
31	20912.0
32	23761.5
33	26611.0
34	29198.0
35	31785.0
36	32669.5
37	33554.0
38	35944.5
39	38335.0
40	45042.5
41	51750.0
42	58125.0
43	64500.0
44	73983.0
45	83466.0
46	92754.5
47	102043.0
48	113090.5
49	124138.0
50	120591.0
51	117044.0
52	101414.5
53	85785.0
54	76736.5
55	67688.0
56	61604.5
57	55521.0
58	53433.5
59	51346.0
60	49458.0
61	47570.0
62	42641.5
63	37713.0
64	32422.0
65	27131.0
66	22109.5
67	17088.0
68	14305.0
69	11522.0
70	9864.5
71	8207.0
72	6529.5
73	4852.0
74	4487.0
75	2933.5
76	1745.0
77	1280.0
78	815.0
79	594.5
80	374.0
81	273.5
82	173.0
83	127.5
84	82.0
85	48.0
86	14.0
87	13.0
88	12.0
89	6.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1185835.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.185419862964178
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.57304696206525	16.584679320603087
2	13.187680540890007	6.378991818001348
3	5.591579155991728	4.057040687539764
4	2.922543082990344	2.8273172611889295
5	1.7004081615142779	2.056254266231691
6	1.0676583299266822	1.5493058987680766
7	0.7050142480367605	1.1935745918698715
8	0.527991499976335	1.0215756888803122
9	0.3984438581151978	0.8672878800301848
>10	3.1766878528864644	18.00739538081832
>50	1.7940343339255143	31.454773019923493
>100	0.34378371470823027	11.10535979214284
>500	0.008743632050382722	1.4791254217935315
>1k	0.0023846269228316517	1.4173189722085793
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	3708	0.3126910573562089	No Hit
CTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGCT	3532	0.29784919487112455	No Hit
CCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	3192	0.26917741507039344	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1617	0.13635961158171248	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1472	0.12413194078434184	No Hit
TCTGTCTCTTATACACATCTGACGCAGAAGCAGTCGTATGCCGTCTTCTGC	1224	0.10321840728263207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.432876411979745E-5	0.0	0.0	0.3255933582665379	0.0
2	8.432876411979745E-5	0.0	0.0	1.163652616089085	0.0
3	8.432876411979745E-5	0.0	0.0	1.6283884351532887	0.0
4	8.432876411979745E-5	0.0	0.0	2.166152963945237	0.0
5	8.432876411979745E-5	0.0	0.0	3.5588425033836915	0.0
6	8.432876411979745E-5	0.0	0.0	4.526346414130128	0.0
7	1.686575282395949E-4	0.0	0.0	5.319289783148583	0.0
8	1.686575282395949E-4	0.0	0.0	6.683982172899265	0.0
9	1.686575282395949E-4	0.0	0.0	7.167017333777465	0.0
10	1.686575282395949E-4	0.0	0.0	8.217247762125423	0.0
11	1.686575282395949E-4	0.0	0.0	9.845383210986352	0.0
12	1.686575282395949E-4	0.0	0.0	11.15306935619205	0.0
13	1.686575282395949E-4	0.0	0.0	11.697495857349463	0.0
14	1.686575282395949E-4	0.0	0.0	11.932857438007817	0.0
15	1.686575282395949E-4	0.0	0.0	12.361163230972268	0.0
16	2.529862923593923E-4	0.0	0.0	13.2306771178115	0.0
17	2.529862923593923E-4	0.0	0.0	14.2798112722259	0.0
18	2.529862923593923E-4	0.0	0.0	15.547272596946456	0.0
19	2.529862923593923E-4	0.0	0.0	16.196604080668894	0.0
20	2.529862923593923E-4	0.0	0.0	16.79854279895601	0.0
21	2.529862923593923E-4	0.0	0.0	17.464149734153573	0.0
22	2.529862923593923E-4	0.0	0.0	18.17217403770339	0.0
23	2.529862923593923E-4	0.0	0.0	18.841491438522223	0.0
24	2.529862923593923E-4	0.0	0.0	19.376473118098218	0.0
25	2.529862923593923E-4	0.0	0.0	19.872326251122626	0.0
26	2.529862923593923E-4	0.0	0.0	20.333941905914397	0.0
27	2.529862923593923E-4	0.0	0.0	20.760814109888813	0.0
28	2.529862923593923E-4	0.0	0.0	21.21138269658089	0.0
29	2.529862923593923E-4	0.0	0.0	21.69028574801722	0.0
30	2.529862923593923E-4	0.0	0.0	22.20249866128087	0.0
31	2.529862923593923E-4	0.0	0.0	22.728035519275448	0.0
32	2.529862923593923E-4	0.0	0.0	23.259306733230172	0.0
33	2.529862923593923E-4	0.0	0.0	23.775736084699812	0.0
34	2.529862923593923E-4	0.0	0.0	24.285587792568105	0.0
35	2.529862923593923E-4	0.0	0.0	24.863745799373437	0.0
36	2.529862923593923E-4	0.0	0.0	25.44957772371367	0.0
37	2.529862923593923E-4	0.0	0.0	26.025458853887766	0.0
38	3.373150564791898E-4	0.0	0.0	26.551417355702945	0.0
39	3.373150564791898E-4	0.0	0.0	27.087748295504856	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTAT	30	2.1655596E-6	45.000004	3
GGTACGA	30	2.1655596E-6	45.000004	8
CTCGTAG	45	3.8562575E-10	45.000004	1
TATTGCG	30	2.1655596E-6	45.000004	1
GAACGTA	30	2.1655596E-6	45.000004	2
ACGTATC	30	2.1655596E-6	45.000004	11
CCGAACG	30	2.1655596E-6	45.000004	1
CACGTCG	30	2.1655596E-6	45.000004	18
AGCGTAC	35	1.2119744E-7	45.0	32
CAAACGT	20	7.0333766E-4	45.0	36
CGTATCA	20	7.0333766E-4	45.0	39
CGAGTTA	20	7.0333766E-4	45.0	26
GAGTCAT	25	3.890881E-5	45.0	7
GCGCGAA	40	6.8157533E-9	45.0	20
ATTCGTT	35	1.2119744E-7	45.0	11
CGTTCGA	25	3.890881E-5	45.0	40
CCCCGTA	20	7.0333766E-4	45.0	36
TCAATCG	25	3.890881E-5	45.0	18
TCGGACA	20	7.0333766E-4	45.0	35
TAACACG	20	7.0333766E-4	45.0	1
>>END_MODULE