FastQCFastQC Report
Sat 18 Jun 2016
SRR3550101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences900339
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT73380.8150263400785704Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC61450.6825206949826677No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC60080.6673042043052673No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC27920.3101054158489191No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC24330.270231546117629No Hit
ACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC18880.20969879123307997No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG18120.20125752633174837No Hit
TGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCTT16630.18470820435413773Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC15730.1747119696025608No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG14950.16604856615119415No Hit
AGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG13990.15538591574951213No Hit
GGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG13330.14805534359835573No Hit
ACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG12880.14305722622256728No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC11520.1279518048201844No Hit
CGTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT9550.10607115764173272No Hit
GGCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT9310.1034054950413122No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACTA253.8901293E-545.00000421
CGTTATG302.1649757E-645.0000041
CAATGCG253.8901293E-545.0000041
CCTATCG502.1827873E-1145.00000429
GCGTAAG302.1649757E-645.0000041
TACGATG207.0324726E-445.01
TTCGAAG453.8380676E-1045.01
TTAACGG700.041.7857132
CGGTCAA2000.041.62500429
TACCGAT1300.041.53846431
CTCGACC2450.041.3265328
CGCTCGA2550.040.58823426
TTACGCT950.040.26315730
GCGATCG1800.040.031
CTGATCG900.040.030
GTCGCTC1700.039.70588330
CGACCGA2550.039.70588330
TAACCGA850.039.70588331
TCGACCG2550.039.70588329
ACGGTCA2000.039.37500428