##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	900339
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.756047444351516	33.0	31.0	34.0	30.0	34.0
2	32.09658917363349	33.0	31.0	34.0	30.0	34.0
3	32.15044777578223	34.0	31.0	34.0	30.0	34.0
4	35.73275843876584	37.0	35.0	37.0	35.0	37.0
5	30.207364115072213	37.0	35.0	37.0	0.0	37.0
6	32.681347803438484	37.0	35.0	37.0	17.0	37.0
7	34.92462505789486	37.0	35.0	37.0	32.0	37.0
8	35.513578774217265	37.0	35.0	37.0	33.0	37.0
9	37.36616207895026	39.0	37.0	39.0	34.0	39.0
10	37.24519764222143	39.0	37.0	39.0	34.0	39.0
11	37.15581908592208	39.0	37.0	39.0	34.0	39.0
12	37.106823096633605	39.0	37.0	39.0	34.0	39.0
13	37.10282571342572	39.0	37.0	39.0	33.0	39.0
14	38.219683919057154	40.0	38.0	41.0	33.0	41.0
15	38.30243275033071	40.0	38.0	41.0	34.0	41.0
16	38.37108355852629	40.0	38.0	41.0	34.0	41.0
17	38.36835236505361	40.0	38.0	41.0	34.0	41.0
18	38.32158775750023	40.0	37.0	41.0	34.0	41.0
19	38.33150402237379	40.0	37.0	41.0	34.0	41.0
20	38.268742107139644	40.0	37.0	41.0	34.0	41.0
21	38.22489306805548	40.0	37.0	41.0	34.0	41.0
22	38.22795635866046	40.0	37.0	41.0	34.0	41.0
23	38.166391770211	40.0	37.0	41.0	34.0	41.0
24	38.14847518545792	40.0	37.0	41.0	34.0	41.0
25	38.09299275050842	40.0	37.0	41.0	34.0	41.0
26	38.03481466425424	40.0	37.0	41.0	34.0	41.0
27	38.01784994318807	40.0	37.0	41.0	34.0	41.0
28	37.905436729942835	40.0	37.0	41.0	33.0	41.0
29	37.9293432806976	40.0	37.0	41.0	33.0	41.0
30	37.78854631422164	40.0	37.0	41.0	33.0	41.0
31	33.1543740746541	38.0	33.0	40.0	9.0	41.0
32	34.08256667766253	38.0	33.0	40.0	16.0	41.0
33	36.00193593746356	38.0	34.0	40.0	30.0	41.0
34	36.88295297660103	38.0	35.0	40.0	31.0	41.0
35	37.079411199559274	39.0	35.0	40.0	31.0	41.0
36	37.19971810618001	39.0	36.0	40.0	32.0	41.0
37	37.30848047235541	39.0	36.0	41.0	32.0	41.0
38	37.27941586446883	39.0	36.0	41.0	32.0	41.0
39	37.27695790141269	39.0	36.0	41.0	32.0	41.0
40	37.32223529137358	39.0	36.0	41.0	32.0	41.0
41	37.28502597354996	39.0	36.0	41.0	32.0	41.0
42	37.20389986438442	39.0	36.0	41.0	32.0	41.0
43	37.18954749266665	39.0	35.0	41.0	32.0	41.0
44	37.105059316546324	39.0	35.0	41.0	32.0	41.0
45	37.127092128631546	39.0	35.0	41.0	32.0	41.0
46	37.06396257409709	39.0	35.0	41.0	32.0	41.0
47	36.93545320151632	39.0	35.0	41.0	31.0	41.0
48	36.971876148872816	39.0	35.0	41.0	31.0	41.0
49	36.936580554657745	39.0	35.0	41.0	31.0	41.0
50	36.74694976003483	39.0	35.0	40.0	31.0	41.0
51	36.06357272094178	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	5.0
11	2.0
12	5.0
13	6.0
14	19.0
15	18.0
16	46.0
17	76.0
18	145.0
19	213.0
20	393.0
21	609.0
22	1102.0
23	1692.0
24	2482.0
25	3404.0
26	4485.0
27	5768.0
28	7887.0
29	10231.0
30	13840.0
31	19740.0
32	28136.0
33	35530.0
34	60318.0
35	84712.0
36	110865.0
37	110754.0
38	180747.0
39	216982.0
40	126.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.79841037653595	21.992382869119297	23.75538547147241	13.453821282872342
2	31.969069428293125	23.738058664569678	25.12742422576385	19.165447681373347
3	30.57437254189811	23.57900746274459	27.93781009153219	17.908809903825116
4	28.85235450202646	24.765227319931714	27.145775091382244	19.23664308665958
5	21.723595223576897	37.997798606969155	21.781018038760955	18.497588130692993
6	26.239338737964257	31.11605739615856	25.970440023146836	16.67416384273035
7	74.70985928633547	5.898667057630515	11.855756553920244	7.535717102113759
8	71.99854721388277	4.983567300761157	13.267780247217992	9.75010523813808
9	65.45567836115063	7.102657998820444	16.599969567018647	10.841694073010277
10	35.30192516374388	22.945690456594683	23.704071466414316	18.048312913247123
11	25.13464372863999	24.526095170818994	28.299340581714223	22.039920518826797
12	23.135952124699696	21.17102558036473	33.7927158548058	21.900306440129775
13	22.181644913749153	22.47675597747071	35.32247297962212	20.019126129158018
14	17.84305689301474	27.203531114391353	31.36274225597247	23.59066973662143
15	16.157802783173896	26.089173078140565	36.17126437930602	21.581759759379523
16	17.196855850962802	27.40045693899742	32.202092767279886	23.200594442759893
17	17.2069631549894	27.01015950658585	29.067162479910348	26.715714858514406
18	17.514402908237898	26.528007783734793	32.778875512445865	23.178713795581444
19	18.62454031203802	27.28616665500439	30.449197468953358	23.640095564004223
20	20.210054212913136	28.22136995065192	29.682930540607483	21.88564529582746
21	19.422795191588946	28.287678307837382	31.190584879695315	21.09894162087836
22	17.54772369074315	25.901466003360955	28.362316860649155	28.18849344524674
23	18.227800861675437	26.45636810134849	29.775895523797146	25.539935513178925
24	20.794389668780315	24.165786442662153	29.095485145039813	25.94433874351772
25	18.2427952138028	26.893314629267422	27.112454308876988	27.75143584805279
26	16.866091549960625	26.50745996785655	30.67866659113956	25.947781891043263
27	20.56014456776836	26.15259363417557	28.093529215106756	25.193732582949313
28	15.914561070885522	27.682128620441855	28.752058946685636	27.651251361986983
29	17.309591165105587	25.27181428328663	29.920840927695014	27.497753623912768
30	19.640490970623286	25.619905391191537	25.89802285583541	28.84158078234976
31	20.366550821412822	30.284148526277328	22.51785160922719	26.83144904308266
32	18.495033537367593	30.556601457895304	24.489331240788193	26.459033763948913
33	18.541904771424985	27.782646314332716	23.34465129245762	30.330797621784683
34	17.14831857778015	26.604090237121795	26.332192651878906	29.915398533219157
35	19.357486457878643	25.285920081213852	26.017977672854336	29.33861578805317
36	17.730432648146977	28.26535338355886	25.67377398957504	28.33043997871913
37	17.848277148940568	29.310293122923696	26.91874949324643	25.9226802348893
38	19.173333599899596	30.118322098676163	24.08925971217508	26.61908458924916
39	20.322456319230866	26.316198676276382	25.807834604521183	27.553510399971564
40	20.972322647358386	21.428706298405377	29.835872932306607	27.76309812192963
41	20.196614830636015	24.687256688869414	25.60513317761421	29.51099530288036
42	21.076172419499766	25.73930486183538	28.17705330991993	25.007469408744925
43	21.160473999238064	24.74001459450274	29.114255852517772	24.98525555374142
44	18.977629537318723	24.62561324123469	26.372066521610193	30.02469069983639
45	19.878068149885767	20.74440849502243	27.364692632441777	32.01283072265003
46	22.347027064250245	21.79745629146355	28.027331927196315	27.828184717089897
47	17.296596059928536	23.35431431938414	31.674069433846586	27.675020186840733
48	17.919028277126728	22.345583163675016	30.116767128825924	29.61862143037234
49	20.66166188513438	20.48628349988171	31.877992622778756	26.974061992205158
50	19.094585483912173	18.866671331576217	31.652855202318236	30.38588798219337
51	18.22346915994975	18.65541757049289	27.501418909988352	35.619694359569
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	432.0
1	646.5
2	861.0
3	1172.5
4	1484.0
5	1215.5
6	947.0
7	949.0
8	951.0
9	1001.0
10	1051.0
11	1133.0
12	1215.0
13	1255.5
14	1296.0
15	1315.0
16	1334.0
17	1437.0
18	1540.0
19	1530.0
20	1520.0
21	1837.5
22	2155.0
23	2491.5
24	2828.0
25	3718.0
26	5586.5
27	6565.0
28	7482.0
29	8399.0
30	9597.5
31	10796.0
32	11936.5
33	13077.0
34	14434.0
35	15791.0
36	18036.5
37	20282.0
38	22790.0
39	25298.0
40	29374.0
41	33450.0
42	39806.0
43	46162.0
44	55960.5
45	65759.0
46	84780.0
47	103801.0
48	123740.0
49	143679.0
50	137790.5
51	131902.0
52	106930.5
53	81959.0
54	66572.5
55	51186.0
56	42167.5
57	33149.0
58	28812.0
59	24475.0
60	22283.5
61	20092.0
62	17853.0
63	15614.0
64	12775.5
65	9937.0
66	8038.0
67	6139.0
68	5222.5
69	4306.0
70	3525.5
71	2745.0
72	2177.0
73	1609.0
74	1270.0
75	715.0
76	499.0
77	394.5
78	290.0
79	210.0
80	130.0
81	101.0
82	72.0
83	46.5
84	21.0
85	11.5
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	900339.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.47611917885358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.06619460950286	25.13380702057917
2	14.107303717542289	10.57373990821798
3	5.804549185756551	6.525959311947902
4	2.9286767786599754	4.390217599736089
5	1.8205559655604504	3.4113686168558153
6	1.2148059339951092	2.7315727176947546
7	0.9361891375831781	2.4559314985810734
8	0.7848523603673508	2.35305764759451
9	0.6547480896920452	2.208367569728322
>10	4.5234876335112935	27.29720821103423
>50	0.09088219941556652	2.3260298768457486
>100	0.05830786310573859	4.605788598390737
>500	0.004886133779797826	1.3091382472124382
>1k	0.0035831647718517394	2.2984385414505577
>5k	9.772267559595652E-4	2.3793746341307003
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	7338	0.8150263400785704	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	6145	0.6825206949826677	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	6008	0.6673042043052673	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCC	2792	0.3101054158489191	No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	2433	0.270231546117629	No Hit
ACTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	1888	0.20969879123307997	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1812	0.20125752633174837	No Hit
TGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCTT	1663	0.18470820435413773	Illumina Single End Adapter 2 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTC	1573	0.1747119696025608	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1495	0.16604856615119415	No Hit
AGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1399	0.15538591574951213	No Hit
GGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1333	0.14805534359835573	No Hit
ACCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	1288	0.14305722622256728	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTC	1152	0.1279518048201844	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	955	0.10607115764173272	No Hit
GGCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCT	931	0.1034054950413122	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1106927501752118E-4	0.0	0.0	0.8580101495103511	0.0
2	1.1106927501752118E-4	0.0	0.0	2.7967243449411834	0.0
3	1.1106927501752118E-4	0.0	0.0	4.069800375192011	0.0
4	1.1106927501752118E-4	0.0	0.0	5.470383933162953	0.0
5	1.1106927501752118E-4	0.0	0.0	8.808015647439465	0.0
6	1.1106927501752118E-4	0.0	0.0	11.046839023967639	0.0
7	1.1106927501752118E-4	0.0	0.0	13.037422570831653	0.0
8	1.1106927501752118E-4	0.0	0.0	16.38105202595911	0.0
9	1.1106927501752118E-4	0.0	0.0	17.59503920190062	0.0
10	1.1106927501752118E-4	0.0	0.0	20.00557567760588	0.0
11	1.1106927501752118E-4	0.0	0.0	23.737725456744627	0.0
12	1.1106927501752118E-4	0.0	0.0	26.476027363026592	0.0
13	1.1106927501752118E-4	0.0	0.0	27.577057086275282	0.0
14	1.1106927501752118E-4	0.0	0.0	28.050545405674974	0.0
15	1.1106927501752118E-4	0.0	0.0	28.868681685454035	0.0
16	1.1106927501752118E-4	0.0	0.0	30.501066820386544	0.0
17	1.1106927501752118E-4	0.0	0.0	32.31804909039817	0.0
18	1.1106927501752118E-4	0.0	0.0	34.326959067640075	0.0
19	1.1106927501752118E-4	0.0	0.0	35.58170866751301	0.0
20	1.1106927501752118E-4	0.0	0.0	36.687625438862476	0.0
21	1.1106927501752118E-4	0.0	0.0	37.98047180006642	0.0
22	1.1106927501752118E-4	0.0	0.0	39.29419918497366	0.0
23	1.1106927501752118E-4	0.0	0.0	40.48075224998584	0.0
24	1.1106927501752118E-4	0.0	0.0	41.446721734813224	0.0
25	1.1106927501752118E-4	0.0	0.0	42.324724353826724	0.0
26	1.1106927501752118E-4	0.0	0.0	43.11764790817681	0.0
27	1.1106927501752118E-4	0.0	0.0	43.83993140361575	0.0
28	1.1106927501752118E-4	0.0	0.0	44.59264788040949	0.0
29	2.2213855003504237E-4	0.0	0.0	45.35413882992962	0.0
30	2.2213855003504237E-4	0.0	0.0	46.1578361039564	0.0
31	2.2213855003504237E-4	0.0	0.0	46.9166613908761	0.0
32	2.2213855003504237E-4	0.0	0.0	47.78600060643824	0.0
33	2.2213855003504237E-4	0.0	0.0	48.5603755918604	0.0
34	2.2213855003504237E-4	0.0	0.0	49.31231458372902	0.0
35	2.2213855003504237E-4	0.0	0.0	50.09579724970261	0.0
36	2.2213855003504237E-4	0.0	0.0	50.89738420750406	0.0
37	2.2213855003504237E-4	0.0	0.0	51.67786800305218	0.0
38	4.4427710007008473E-4	0.0	0.0	52.427918817245505	0.0
39	4.4427710007008473E-4	0.0	0.0	53.16164244801125	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	25	3.8901293E-5	45.000004	21
CGTTATG	30	2.1649757E-6	45.000004	1
CAATGCG	25	3.8901293E-5	45.000004	1
CCTATCG	50	2.1827873E-11	45.000004	29
GCGTAAG	30	2.1649757E-6	45.000004	1
TACGATG	20	7.0324726E-4	45.0	1
TTCGAAG	45	3.8380676E-10	45.0	1
TTAACGG	70	0.0	41.785713	2
CGGTCAA	200	0.0	41.625004	29
TACCGAT	130	0.0	41.538464	31
CTCGACC	245	0.0	41.32653	28
CGCTCGA	255	0.0	40.588234	26
TTACGCT	95	0.0	40.263157	30
GCGATCG	180	0.0	40.0	31
CTGATCG	90	0.0	40.0	30
GTCGCTC	170	0.0	39.705883	30
CGACCGA	255	0.0	39.705883	30
TAACCGA	85	0.0	39.705883	31
TCGACCG	255	0.0	39.705883	29
ACGGTCA	200	0.0	39.375004	28
>>END_MODULE