FastQCFastQC Report
Sat 18 Jun 2016
SRR3550097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2284759
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT104830.45882300934146664TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC101040.44223482651780777TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC85560.3744815098660296TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC43410.1899981573548895TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC30420.133143145513378TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG23210.10158620668525652TruSeq Adapter, Index 21 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG22900.10022938962052452TruSeq Adapter, Index 21 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAATT207.0347503E-445.0000049
TATCGCG351.2126293E-745.0000041
TTCGCGA253.8920203E-545.026
CGTAAGG3150.043.571432
TTACGCG700.041.7857171
GCGTAAG1150.041.086961
TCGTAAG1300.039.8076931
CTCGTAG800.039.3750041
CGCGACA403.4602272E-739.37500428
GCCGATC403.4602272E-739.3750049
TAACGCA2250.038.030
TCGAAAG1550.037.741941
AAGGGAT43200.037.7083325
TACGGGA13500.037.54
CAACTCG1500.037.514
AGGGCGC2700.037.56
TCGTTAT301.1402751E-437.49999643
CGCGAGT301.1402751E-437.49999626
ACGCATC2650.037.3584929
ATACGCA2650.037.3584927
Produced by FastQC (version 0.11.5)