##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550097_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2284759
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00464162741016	33.0	31.0	34.0	30.0	34.0
2	32.33357435073021	34.0	31.0	34.0	30.0	34.0
3	32.3796063392244	34.0	31.0	34.0	30.0	34.0
4	35.90093703537222	37.0	35.0	37.0	35.0	37.0
5	30.371546408176968	37.0	35.0	37.0	0.0	37.0
6	32.85914663209555	37.0	35.0	37.0	17.0	37.0
7	35.10548596153905	37.0	35.0	37.0	32.0	37.0
8	35.69052272033943	37.0	35.0	37.0	35.0	37.0
9	37.547596048423486	39.0	37.0	39.0	35.0	39.0
10	37.40923353404013	39.0	37.0	39.0	35.0	39.0
11	37.2933989974435	39.0	37.0	39.0	34.0	39.0
12	37.301364388979316	39.0	37.0	39.0	34.0	39.0
13	37.344628470661455	39.0	37.0	39.0	34.0	39.0
14	38.56811987610072	40.0	38.0	41.0	35.0	41.0
15	38.6546699236112	40.0	38.0	41.0	35.0	41.0
16	38.69677370786153	40.0	38.0	41.0	35.0	41.0
17	38.6710664013141	40.0	38.0	41.0	35.0	41.0
18	38.629752197058856	40.0	38.0	41.0	35.0	41.0
19	38.641789790520576	40.0	38.0	41.0	35.0	41.0
20	38.57589356251578	40.0	38.0	41.0	34.0	41.0
21	38.53784709897192	40.0	38.0	41.0	34.0	41.0
22	38.53504592825764	40.0	38.0	41.0	34.0	41.0
23	38.457664462641354	40.0	38.0	41.0	34.0	41.0
24	38.44576255088611	40.0	38.0	41.0	34.0	41.0
25	38.36654544308612	40.0	38.0	41.0	34.0	41.0
26	38.281601254224185	40.0	37.0	41.0	34.0	41.0
27	38.23430348671348	40.0	37.0	41.0	34.0	41.0
28	38.19834039388837	40.0	37.0	41.0	34.0	41.0
29	38.19466517037464	40.0	37.0	41.0	34.0	41.0
30	38.06432100716093	40.0	37.0	41.0	34.0	41.0
31	33.47510831558164	39.0	34.0	41.0	9.0	41.0
32	34.37005303403991	39.0	34.0	41.0	16.0	41.0
33	36.36681505576737	39.0	34.0	41.0	30.0	41.0
34	37.24358805458256	39.0	35.0	41.0	32.0	41.0
35	37.29004547087899	39.0	36.0	41.0	32.0	41.0
36	37.46791674745564	39.0	36.0	41.0	33.0	41.0
37	37.62264947856645	40.0	37.0	41.0	33.0	41.0
38	37.60845585902058	40.0	37.0	41.0	33.0	41.0
39	37.68396141562414	40.0	37.0	41.0	33.0	41.0
40	37.65688985140227	40.0	37.0	41.0	33.0	41.0
41	37.52711511367282	40.0	36.0	41.0	33.0	41.0
42	37.43257078755352	40.0	36.0	41.0	33.0	41.0
43	37.47235528998901	40.0	36.0	41.0	33.0	41.0
44	37.45317033437662	40.0	36.0	41.0	33.0	41.0
45	37.43901348019638	40.0	36.0	41.0	33.0	41.0
46	37.36240758872161	39.0	36.0	41.0	33.0	41.0
47	37.24421919335912	39.0	35.0	41.0	32.0	41.0
48	37.233146690745066	39.0	35.0	41.0	32.0	41.0
49	37.239065914610684	39.0	35.0	41.0	32.0	41.0
50	37.075656119529455	39.0	35.0	41.0	32.0	41.0
51	36.450009825981645	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	12.0
11	17.0
12	15.0
13	12.0
14	26.0
15	45.0
16	82.0
17	149.0
18	281.0
19	485.0
20	893.0
21	1580.0
22	2524.0
23	4036.0
24	6001.0
25	8225.0
26	10570.0
27	13471.0
28	16910.0
29	22044.0
30	29482.0
31	41221.0
32	61261.0
33	78113.0
34	132776.0
35	198653.0
36	273492.0
37	277079.0
38	432492.0
39	672194.0
40	613.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.90398987376787	22.185622203479667	26.190508495644398	11.719879427108067
2	31.08896824566617	25.14532167287666	25.872881997619878	17.89282808383729
3	31.17523555000768	24.555368859472708	26.835171674561735	17.434223915957876
4	28.840766137697678	25.6919001085016	26.279708275577423	19.187625478223307
5	22.535812311057754	39.21927870729473	20.914590991872668	17.33031798977485
6	26.867691515822894	32.55187965120172	24.15020577662677	16.430223056348613
7	80.5569427672678	5.107715956037376	8.485227544787	5.850113731907829
8	80.6341938033727	4.260580656428096	8.672555836304836	6.432669703894371
9	73.74186949258106	7.629688733034863	11.168836625657235	7.459605148726846
10	36.13238858015222	32.47007671268611	17.830326962274796	13.567207744886877
11	22.61717756664926	24.018594521347765	32.60063752894725	20.763590383055718
12	21.612213804606963	22.21411536183904	35.08019883059876	21.093472002955235
13	21.760544547586857	23.39445867157105	35.184411134828665	19.66058564601343
14	18.19075885027699	28.19500875147007	32.01584937404777	21.598383024205177
15	17.13467372269898	27.831556851291538	34.79045273483987	20.243316691169618
16	20.074283545879457	28.15833092242989	30.60125816333364	21.166127368357014
17	19.691004609238874	26.680932212106395	29.853389350911847	23.77467382774288
18	20.031565692486602	25.74197103501945	32.55485589508565	21.671607377408293
19	20.039706594874996	27.074628002340727	30.46229383492964	22.42337156785464
20	21.118988917430677	28.14585695909284	30.514947090699717	20.22020703277676
21	21.16153169765389	27.77675894919333	31.582499510889335	19.47920984226345
22	20.020492314506694	24.849623089349908	30.173992092820296	24.955892503323106
23	18.97390490638181	26.505377591247044	30.75751096723987	23.763206535131275
24	20.550964018524493	25.61618096263107	30.969524575677347	22.86333044316709
25	18.779880066125138	28.53872115177137	28.380411238121834	24.300987543981662
26	18.745084273658623	29.796753180532388	28.77095571130259	22.687206834506394
27	20.91113329677222	28.621049309795914	28.744563430978936	21.723253962452933
28	18.94436130900458	27.733253266537083	29.39329706109047	23.929088363367864
29	19.46853037891524	27.234294733055	28.672827199717783	24.624347688311985
30	22.53134794523186	28.4652779571062	27.21661234292107	21.786761754740873
31	25.15481939232978	28.61395884642538	24.48656510380307	21.74465665744177
32	20.82346540707357	29.058469624148543	26.982758356570653	23.13530661220724
33	22.912788613591193	26.4532933232783	27.86897874130269	22.764939321827814
34	23.207480526392498	25.34971959843467	28.279919238746842	23.162880636425985
35	20.398081373133884	26.416440421068483	29.193232196481116	23.992246009316517
36	22.56229212796623	27.569647389505853	29.79176359519757	20.07629688733035
37	22.38629982418277	26.487607664528294	30.504661541983204	20.621430969305734
38	21.2776489774195	25.562214658088667	28.9544761613807	24.205660203111137
39	23.792706364216095	23.54550304868041	29.865775777664076	22.79601480943942
40	24.23025798344596	23.77765007162681	30.630801760710867	21.361290184216365
41	20.656795749573586	26.046685886782804	29.909456533489966	23.38706183015364
42	20.98278199144855	25.292558208546286	31.33013153684918	22.394528263155983
43	22.2944739467051	24.64255529795484	30.410297103545712	22.65267365179435
44	21.884496351693986	24.601325566503952	27.863595241336174	25.650582840465887
45	22.946358893870205	22.868013650455037	27.64781755975138	26.537809895923374
46	24.246671093099973	24.182594313010693	29.183209257519067	22.38752533637027
47	20.528335811348157	24.4445913113812	32.2763144821839	22.750758395086745
48	20.05292461918303	23.340098452396948	32.088548507741955	24.518428420678067
49	22.234248776347965	21.619785719194017	33.139950428032016	23.006015076426003
50	21.569277109752058	21.561048670778842	31.78829802180449	25.08137619766461
51	20.357902080700853	21.030358125298992	29.65433115702794	28.957408636972215
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1316.0
1	1920.5
2	2525.0
3	3467.0
4	4409.0
5	3572.0
6	2735.0
7	2759.5
8	2784.0
9	3111.5
10	3439.0
11	3636.0
12	3833.0
13	4190.5
14	4548.0
15	4683.0
16	4818.0
17	4992.5
18	5167.0
19	6122.0
20	7077.0
21	7675.0
22	8273.0
23	9668.0
24	11063.0
25	14260.5
26	21192.5
27	24927.0
28	28763.5
29	32600.0
30	38424.0
31	44248.0
32	48695.5
33	53143.0
34	61143.5
35	69144.0
36	73154.0
37	77164.0
38	85106.0
39	93048.0
40	99098.0
41	105148.0
42	119749.0
43	134350.0
44	154016.0
45	173682.0
46	203693.5
47	233705.0
48	263511.0
49	293317.0
50	279686.5
51	266056.0
52	219364.5
53	172673.0
54	145452.0
55	118231.0
56	102666.0
57	87101.0
58	76940.0
59	66779.0
60	57853.0
61	48927.0
62	43415.5
63	37904.0
64	32782.0
65	27660.0
66	22546.5
67	17433.0
68	14461.5
69	11490.0
70	9255.5
71	7021.0
72	5632.0
73	4243.0
74	3382.0
75	2009.0
76	1497.0
77	1133.0
78	769.0
79	556.5
80	344.0
81	236.5
82	129.0
83	86.5
84	44.0
85	24.5
86	5.0
87	4.0
88	3.0
89	3.5
90	4.0
91	4.0
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2284759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.654681156465436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.05185168019865	16.43229832336711
2	14.390957017758774	7.383908276540001
3	6.535872403216339	5.0302716775156995
4	3.439690181906583	3.529766195753519
5	2.156189917901832	2.7658182428278444
6	1.3517617238228261	2.0807409614513217
7	0.9307950384274655	1.6715474953013634
8	0.6634907337726982	1.361731458016629
9	0.5057650967720367	1.1677718067979939
>10	4.641243300118935	29.27783153632929
>50	1.2196925293394314	20.452055541293905
>100	0.10107883367557981	4.406030620221131
>500	0.006852713954147688	1.164898107010159
>1k	0.004187769638645809	1.8521459924781714
>5k	1.9035316539299133E-4	0.41782796191745286
>10k+	3.8070633078598267E-4	1.0053558031784249
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGCT	10483	0.45882300934146664	TruSeq Adapter, Index 14 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	10104	0.44223482651780777	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	8556	0.3744815098660296	TruSeq Adapter, Index 21 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	4341	0.1899981573548895	TruSeq Adapter, Index 21 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTGC	3042	0.133143145513378	TruSeq Adapter, Index 14 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG	2321	0.10158620668525652	TruSeq Adapter, Index 21 (95% over 22bp)
GGCTGTCTCTTATACACATCTGACGCAAGCGGTTTCGTATGCCGTCTTCTG	2290	0.10022938962052452	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.75365848214188E-5	0.0	0.0	0.487535009162892	0.0
2	8.75365848214188E-5	0.0	0.0	1.7017112089283815	0.0
3	8.75365848214188E-5	0.0	0.0	2.4558388871649046	0.0
4	8.75365848214188E-5	0.0	0.0	3.3787808692295336	0.0
5	8.75365848214188E-5	0.0	0.0	5.428362466238234	0.0
6	8.75365848214188E-5	0.0	0.0	6.7582620311376385	0.0
7	8.75365848214188E-5	0.0	0.0	7.887046292409834	0.0
8	8.75365848214188E-5	0.0	0.0	9.795300073224354	0.0
9	1.313048772321282E-4	0.0	0.0	10.488808666472044	0.0
10	1.313048772321282E-4	0.0	0.0	11.852847499451801	0.0
11	1.313048772321282E-4	0.0	0.0	14.159173899741724	0.0
12	1.313048772321282E-4	0.0	0.0	16.031625217364283	0.0
13	1.313048772321282E-4	0.0	0.0	16.740540249540544	0.0
14	1.313048772321282E-4	0.0	0.0	17.02910460140435	0.0
15	1.750731696428376E-4	0.0	0.0	17.562114866381968	0.0
16	2.18841462053547E-4	0.0	0.0	18.634831945076044	0.0
17	2.626097544642564E-4	0.0	0.0	20.044739948502226	0.0
18	3.501463392856752E-4	0.0	0.0	21.518987341772153	0.0
19	3.501463392856752E-4	0.0	0.0	22.52469516478543	0.0
20	3.501463392856752E-4	0.0	0.0	23.35112806208445	0.0
21	3.501463392856752E-4	0.0	0.0	24.28615009285443	0.0
22	3.501463392856752E-4	0.0	0.0	25.277064233032892	0.0
23	3.939146316963846E-4	0.0	0.0	26.205477251648862	0.0
24	3.939146316963846E-4	0.0	0.0	26.929404808121994	0.0
25	3.939146316963846E-4	0.0	0.0	27.56592708465094	0.0
26	3.939146316963846E-4	0.0	0.0	28.163320507764713	0.0
27	3.939146316963846E-4	0.0	0.0	28.74915910168206	0.0
28	3.939146316963846E-4	0.0	0.0	29.367211158813685	0.0
29	3.939146316963846E-4	0.0	0.0	30.029644264449775	0.0
30	3.939146316963846E-4	0.0	0.0	30.72039545527559	0.0
31	4.37682924107094E-4	0.0	0.0	31.38002739019739	0.0
32	4.37682924107094E-4	0.0	0.0	32.05493445917053	0.0
33	4.37682924107094E-4	0.0	0.0	32.72003743064367	0.0
34	4.37682924107094E-4	0.0	0.0	33.40041553616815	0.0
35	4.814512165178034E-4	0.0	0.0	34.115545665866726	0.0
36	4.814512165178034E-4	0.0	0.0	34.7942605762796	0.0
37	4.814512165178034E-4	0.0	0.0	35.457131364839796	0.0
38	4.814512165178034E-4	0.0	0.0	36.134095543556235	0.0
39	5.252195089285128E-4	0.0	0.0	36.79836691747357	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAATT	20	7.0347503E-4	45.000004	9
TATCGCG	35	1.2126293E-7	45.000004	1
TTCGCGA	25	3.8920203E-5	45.0	26
CGTAAGG	315	0.0	43.57143	2
TTACGCG	70	0.0	41.785717	1
GCGTAAG	115	0.0	41.08696	1
TCGTAAG	130	0.0	39.807693	1
CTCGTAG	80	0.0	39.375004	1
CGCGACA	40	3.4602272E-7	39.375004	28
GCCGATC	40	3.4602272E-7	39.375004	9
TAACGCA	225	0.0	38.0	30
TCGAAAG	155	0.0	37.74194	1
AAGGGAT	4320	0.0	37.708332	5
TACGGGA	1350	0.0	37.5	4
CAACTCG	150	0.0	37.5	14
AGGGCGC	270	0.0	37.5	6
TCGTTAT	30	1.1402751E-4	37.499996	43
CGCGAGT	30	1.1402751E-4	37.499996	26
ACGCATC	265	0.0	37.35849	29
ATACGCA	265	0.0	37.35849	27
>>END_MODULE