FastQCFastQC Report
Sat 18 Jun 2016
SRR3550096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1108651
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC100590.907318894764899TruSeq Adapter, Index 21 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT95770.8638426339758859RNA PCR Primer, Index 21 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC83440.752626390090299RNA PCR Primer, Index 21 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC34210.30857321194857534TruSeq Adapter, Index 21 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC27040.2439000190321391RNA PCR Primer, Index 14 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC23400.21106732416242804No Hit
TGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCTT23140.2087221316717344TruSeq Adapter, Index 21 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21060.18996059174618524No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG18960.17101865239827502TruSeq Adapter, Index 21 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG18080.16308107781438885RNA PCR Primer, Index 21 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG17530.15812009369945998RNA PCR Primer, Index 21 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG16720.15081391709383746RNA PCR Primer, Index 21 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG15850.1429665422211318RNA PCR Primer, Index 14 (95% over 24bp)
AAAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC15720.14179394597578499RNA PCR Primer, Index 21 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC14640.1320523771682883RNA PCR Primer, Index 14 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGCG351.2118835E-745.0000041
CCAGACG351.2118835E-745.0000041
ATCGTCC302.1654323E-645.00000419
GTTCGCG302.1654323E-645.0000041
TATCGCG302.1654323E-645.00000437
GTACGAG302.1654323E-645.0000041
CGCGGTG207.03318E-445.016
ATAGGCG207.03318E-445.010
GACGTAA207.03318E-445.015
CCTACGG453.8562575E-1045.02
GTTAGCG850.045.01
CATTCGC406.8139343E-945.038
TGCGACG207.03318E-445.01
GTCACGA207.03318E-445.018
ACTCGAG207.03318E-445.01
CATGTCG207.03318E-445.02
TAGATCG207.03318E-445.037
GCTACGA406.8139343E-945.010
CGCAACA207.03318E-445.025
GTACGGA207.03318E-445.014
Produced by FastQC (version 0.11.5)