##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1108651
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.709728309449954	33.0	31.0	34.0	30.0	34.0
2	32.01535740282559	33.0	31.0	34.0	30.0	34.0
3	32.084244726248386	33.0	31.0	34.0	30.0	34.0
4	35.69729608325794	37.0	35.0	37.0	33.0	37.0
5	30.21691497143826	37.0	33.0	37.0	0.0	37.0
6	32.686120339042674	37.0	33.0	37.0	17.0	37.0
7	34.89819248798765	37.0	35.0	37.0	32.0	37.0
8	35.4639782943415	37.0	35.0	37.0	33.0	37.0
9	37.29349633022475	39.0	37.0	39.0	34.0	39.0
10	37.165532705964274	39.0	37.0	39.0	34.0	39.0
11	37.094321837981475	39.0	37.0	39.0	34.0	39.0
12	37.003933609404584	39.0	37.0	39.0	33.0	39.0
13	36.9663960975997	39.0	37.0	39.0	33.0	39.0
14	38.06231988245174	40.0	37.0	41.0	33.0	41.0
15	38.189250720019196	40.0	37.0	41.0	33.0	41.0
16	38.247532361401376	40.0	37.0	41.0	34.0	41.0
17	38.245777075021806	40.0	37.0	41.0	34.0	41.0
18	38.19142272906442	40.0	37.0	41.0	34.0	41.0
19	38.1754447522259	40.0	37.0	41.0	34.0	41.0
20	38.09090326892773	40.0	37.0	41.0	34.0	41.0
21	38.01038018276265	40.0	37.0	41.0	34.0	41.0
22	38.00228836667265	40.0	37.0	41.0	34.0	41.0
23	37.906965311897075	40.0	36.0	41.0	33.0	41.0
24	37.90461290342948	40.0	36.0	41.0	33.0	41.0
25	37.842093679616035	40.0	36.0	41.0	33.0	41.0
26	37.797713617720994	40.0	36.0	41.0	33.0	41.0
27	37.738733830574276	40.0	36.0	41.0	33.0	41.0
28	37.73615772682296	40.0	36.0	41.0	33.0	41.0
29	37.75159179940306	40.0	36.0	41.0	33.0	41.0
30	37.564534736359775	40.0	36.0	41.0	33.0	41.0
31	32.96043570068488	38.0	33.0	40.0	9.0	41.0
32	33.90290632489395	38.0	33.0	40.0	16.0	41.0
33	35.79370063257058	38.0	34.0	40.0	29.0	41.0
34	36.57917324748726	38.0	35.0	40.0	30.0	41.0
35	36.81689097831509	39.0	35.0	40.0	31.0	41.0
36	36.98794480860072	39.0	35.0	40.0	31.0	41.0
37	37.038959059253095	39.0	35.0	41.0	32.0	41.0
38	37.06352855858155	39.0	35.0	41.0	32.0	41.0
39	37.05580385531606	39.0	35.0	41.0	32.0	41.0
40	36.965213579386116	39.0	35.0	41.0	31.0	41.0
41	36.918126624158546	39.0	35.0	41.0	31.0	41.0
42	36.914525851688225	39.0	35.0	41.0	31.0	41.0
43	36.93920810065566	39.0	35.0	41.0	31.0	41.0
44	36.845514052664	39.0	35.0	41.0	31.0	41.0
45	36.837351880799275	39.0	35.0	41.0	31.0	41.0
46	36.756219946583734	39.0	35.0	41.0	31.0	41.0
47	36.639038795797774	39.0	35.0	40.0	31.0	41.0
48	36.731020853271225	39.0	35.0	41.0	31.0	41.0
49	36.75636967810429	39.0	35.0	41.0	31.0	41.0
50	36.56244571104883	39.0	35.0	41.0	31.0	41.0
51	35.93954815356681	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	6.0
11	11.0
12	8.0
13	12.0
14	14.0
15	28.0
16	62.0
17	152.0
18	282.0
19	495.0
20	914.0
21	1498.0
22	2405.0
23	3881.0
24	5270.0
25	6256.0
26	7328.0
27	8783.0
28	10586.0
29	13590.0
30	17967.0
31	24717.0
32	36055.0
33	44964.0
34	74368.0
35	106561.0
36	132929.0
37	128173.0
38	208909.0
39	272208.0
40	210.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.343037619593545	21.86224519709088	25.21406646455918	11.580650718756399
2	30.77072947212423	24.270848084744433	25.689779741325268	19.26864270180607
3	29.432706956472327	24.718058252777475	28.343365044545127	17.50586974620507
4	27.20720948251524	27.005793527449125	26.544422004760744	19.242574985274896
5	20.18489136797784	39.38020170459414	22.76451290802967	17.67039401939835
6	25.073805913673464	31.778440645433054	26.90044026479027	16.24731317610321
7	73.78174015086803	7.091320893590498	11.593549277455214	7.533389678086251
8	71.04526131307327	6.104175254430835	12.77733028698842	10.073233145507468
9	64.96679297632889	8.17101143642138	16.25452915299765	10.607666434252078
10	34.136531694825514	23.68202437015797	24.41453622465501	17.76690771036151
11	24.474970031145958	26.90179326045798	27.043857805567306	21.579378902828754
12	22.10010183547392	21.927008589718497	34.07898427909234	21.893905295715243
13	21.765100108149454	26.097843234705962	34.08926704616692	18.047789610977667
14	16.211503890764543	30.44564971302962	30.70551508094071	22.637331315265126
15	14.30513299496415	28.24847494838322	36.67664576138027	20.769746295272363
16	15.281454668782152	29.540856410177774	32.09558282994378	23.082106091096296
17	15.24853177420126	29.1461424740518	28.058694756059392	27.546630995687554
18	16.48138142661667	27.61464157791767	33.29974897420379	22.60422802126188
19	17.251686960098354	28.477221415937027	29.896964869918484	24.37412675404613
20	18.73060142461424	30.102439811987725	29.406729439652334	21.760229323745705
21	17.884167334896194	30.67809436874183	31.25320772722886	20.18453056913312
22	16.034892856273075	27.75264713602387	27.86747136835668	28.344988639346376
23	16.14782289467109	29.00326613154185	29.708898472107094	25.14001250167997
24	19.87541615891746	25.293803009242765	29.40113705755914	25.429643774280635
25	16.263278524982162	29.504235327438483	26.329205493884007	27.903280653695344
26	14.828561918944736	28.377280135949007	29.455888282245716	27.338269662860537
27	18.664304636896553	27.210817470962457	30.52222926782188	23.602648624319105
28	14.503752758983666	25.14380088954955	32.04949077753053	28.302955573936252
29	16.369172985908097	23.563050951110853	28.86931956043877	31.198456502542278
30	20.7278936292846	25.001646144729044	29.290281612518278	24.98017861346808
31	20.13672472220744	29.220196436931005	26.038131025904455	24.604947814957097
32	16.888813522019102	25.908153242093316	27.38625590920858	29.816777326679002
33	20.079447905607807	24.174604992914812	28.446282915002108	27.299664186475276
34	16.21646487487947	24.575091710556343	30.19083552894464	29.01760788561955
35	16.47100845983091	21.934044167190578	30.4184094002531	31.17653797272541
36	19.289118036244048	22.733394007672388	31.200260496765893	26.777227459317675
37	16.36258840699192	25.100685427605264	34.475412009730746	24.061314155672072
38	17.27991946969786	24.214292865834246	30.785792823891377	27.719994840576522
39	22.411290839046732	22.098658640095035	28.20662228239545	27.283428238462783
40	21.917267020910998	23.22020184891368	30.873196344025306	23.989334786150014
41	16.51024533419444	24.296374603008523	29.396446672577753	29.796933390219284
42	18.50898073424369	20.543435219920426	32.24314955743512	28.70443448840077
43	20.546502010100564	19.162658041168953	32.605842596092	27.68499735263848
44	19.04918680450385	20.928317387527727	29.633852312404898	30.388643495563528
45	20.230352022412823	19.390051513055052	28.85777399740766	31.521822467124462
46	23.52814366288399	20.497072568373635	30.935975343006948	25.03880842573542
47	15.912040849645198	21.65460546195331	35.81235212884849	26.621001559553005
48	16.568424147905876	18.78075246403061	34.6437246707936	30.00709871726991
49	20.221873249561856	16.780483668891293	35.40663382795849	27.59100925358837
50	18.741695989089443	17.176550600684976	34.940932719133436	29.140820691092145
51	17.43154518419232	17.325740922977566	29.49665855169932	35.7460553411308
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2553.0
1	2469.0
2	2385.0
3	2433.5
4	2482.0
5	2102.5
6	1723.0
7	1816.5
8	1910.0
9	2204.0
10	2498.0
11	2729.0
12	2960.0
13	3079.5
14	3199.0
15	3237.5
16	3276.0
17	3503.0
18	3730.0
19	3568.5
20	3407.0
21	3797.0
22	4187.0
23	4093.5
24	4000.0
25	4582.0
26	6824.0
27	8484.0
28	9267.0
29	10050.0
30	11749.0
31	13448.0
32	14355.0
33	15262.0
34	18364.5
35	21467.0
36	24055.5
37	26644.0
38	28918.0
39	31192.0
40	36562.5
41	41933.0
42	52017.5
43	62102.0
44	78011.0
45	93920.0
46	116293.0
47	138666.0
48	160157.5
49	181649.0
50	170944.0
51	160239.0
52	127949.0
53	95659.0
54	75738.5
55	55818.0
56	45186.0
57	34554.0
58	28972.5
59	23391.0
60	20464.0
61	17537.0
62	14835.5
63	12134.0
64	10315.5
65	8497.0
66	6629.0
67	4761.0
68	4060.0
69	3359.0
70	2530.0
71	1701.0
72	1409.0
73	1117.0
74	1015.5
75	591.0
76	268.0
77	247.0
78	226.0
79	167.5
80	109.0
81	79.5
82	50.0
83	27.5
84	5.0
85	7.0
86	9.0
87	10.0
88	11.0
89	6.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1108651.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.65390331993557
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.862333676130426	18.299051349413066
2	13.884451093201841	7.956874385499585
3	6.743981589743544	5.797241893918105
4	3.8290095901474412	4.388642824287636
5	2.371373978397247	3.3974560356202854
6	1.5850146740721758	2.725011433892599
7	1.0659054731981576	2.137964666404713
8	0.7759125794070301	1.77863392280407
9	0.6223250369744521	1.6048837298737149
>10	4.625112840382226	29.120220431955495
>50	0.5196501594343361	9.604052094445294
>100	0.09941737764714202	5.715979090680681
>500	0.009166141201498638	1.8378129435297894
>1k	0.005288158385479983	2.8097031391929623
>5k	7.050877847306645E-4	1.810336160116383
>10k+	3.5254389236533227E-4	1.0161358983656434
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	10059	0.907318894764899	TruSeq Adapter, Index 21 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	9577	0.8638426339758859	RNA PCR Primer, Index 21 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	8344	0.752626390090299	RNA PCR Primer, Index 21 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	3421	0.30857321194857534	TruSeq Adapter, Index 21 (96% over 25bp)
ACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	2704	0.2439000190321391	RNA PCR Primer, Index 14 (96% over 25bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	2340	0.21106732416242804	No Hit
TGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCTT	2314	0.2087221316717344	TruSeq Adapter, Index 21 (96% over 27bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2106	0.18996059174618524	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1896	0.17101865239827502	TruSeq Adapter, Index 21 (95% over 24bp)
AGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1808	0.16308107781438885	RNA PCR Primer, Index 21 (96% over 25bp)
GGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1753	0.15812009369945998	RNA PCR Primer, Index 21 (96% over 25bp)
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1672	0.15081391709383746	RNA PCR Primer, Index 21 (96% over 25bp)
ACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1585	0.1429665422211318	RNA PCR Primer, Index 14 (95% over 24bp)
AAAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	1572	0.14179394597578499	RNA PCR Primer, Index 21 (95% over 23bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	1464	0.1320523771682883	RNA PCR Primer, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9102954852338563	0.0
2	0.0	0.0	0.0	3.250617191523753	0.0
3	0.0	0.0	0.0	4.555536413172405	0.0
4	0.0	0.0	0.0	6.029309494151	0.0
5	0.0	0.0	0.0	9.62313658671665	0.0
6	0.0	0.0	0.0	11.936849378208292	0.0
7	0.0	0.0	0.0	13.92367841638171	0.0
8	0.0	0.0	0.0	17.33232550189374	0.0
9	0.0	0.0	0.0	18.47795203359759	0.0
10	0.0	0.0	0.0	20.964036473155215	0.0
11	0.0	0.0	0.0	24.680354773504014	0.0
12	0.0	0.0	0.0	27.452011498659182	0.0
13	0.0	0.0	0.0	28.604132409568024	0.0
14	0.0	0.0	0.0	29.036820424101002	0.0
15	0.0	0.0	0.0	29.876309136058147	0.0
16	0.0	0.0	0.0	31.547078386255006	0.0
17	0.0	0.0	0.0	33.54797857937259	0.0
18	0.0	0.0	0.0	36.014128882759316	0.0
19	0.0	0.0	0.0	37.28838020260659	0.0
20	0.0	0.0	0.0	38.49353854368958	0.0
21	9.01997111805248E-5	0.0	0.0	39.745420335164084	0.0
22	9.01997111805248E-5	0.0	0.0	40.99432553616963	0.0
23	9.01997111805248E-5	0.0	0.0	42.115056947587654	0.0
24	9.01997111805248E-5	0.0	0.0	42.982056571454855	0.0
25	9.01997111805248E-5	0.0	0.0	43.792771575545416	0.0
26	9.01997111805248E-5	0.0	0.0	44.50255310282496	0.0
27	1.803994223610496E-4	0.0	0.0	45.21080123501444	0.0
28	1.803994223610496E-4	0.0	0.0	45.87638490381554	0.0
29	1.803994223610496E-4	0.0	0.0	46.6189991259648	0.0
30	1.803994223610496E-4	0.0	0.0	47.403466014101824	0.0
31	3.607988447220992E-4	0.0	0.0	48.11541233444971	0.0
32	3.607988447220992E-4	0.0	0.0	48.89311424424819	0.0
33	3.607988447220992E-4	0.0	0.0	49.61390013629176	0.0
34	3.607988447220992E-4	0.0	0.0	50.31989327570173	0.0
35	4.50998555902624E-4	0.0	0.0	51.08217103488835	0.0
36	4.50998555902624E-4	0.0	0.0	51.740538726794995	0.0
37	4.50998555902624E-4	0.0	0.0	52.43191951299372	0.0
38	4.50998555902624E-4	0.0	0.0	53.08857341038794	0.0
39	4.50998555902624E-4	0.0	0.0	53.78482498099041	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTAGCG	35	1.2118835E-7	45.000004	1
CCAGACG	35	1.2118835E-7	45.000004	1
ATCGTCC	30	2.1654323E-6	45.000004	19
GTTCGCG	30	2.1654323E-6	45.000004	1
TATCGCG	30	2.1654323E-6	45.000004	37
GTACGAG	30	2.1654323E-6	45.000004	1
CGCGGTG	20	7.03318E-4	45.0	16
ATAGGCG	20	7.03318E-4	45.0	10
GACGTAA	20	7.03318E-4	45.0	15
CCTACGG	45	3.8562575E-10	45.0	2
GTTAGCG	85	0.0	45.0	1
CATTCGC	40	6.8139343E-9	45.0	38
TGCGACG	20	7.03318E-4	45.0	1
GTCACGA	20	7.03318E-4	45.0	18
ACTCGAG	20	7.03318E-4	45.0	1
CATGTCG	20	7.03318E-4	45.0	2
TAGATCG	20	7.03318E-4	45.0	37
GCTACGA	40	6.8139343E-9	45.0	10
CGCAACA	20	7.03318E-4	45.0	25
GTACGGA	20	7.03318E-4	45.0	14
>>END_MODULE