##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550095_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109321
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07724635159706	33.0	31.0	34.0	30.0	34.0
2	32.391927133805275	34.0	31.0	34.0	30.0	34.0
3	32.45094431638813	34.0	31.0	34.0	30.0	34.0
4	35.95076718100532	37.0	35.0	37.0	35.0	37.0
5	30.447848729087433	37.0	35.0	37.0	0.0	37.0
6	32.90984575249184	37.0	35.0	37.0	17.0	37.0
7	35.08415958951467	37.0	35.0	37.0	32.0	37.0
8	35.628516002131036	37.0	35.0	37.0	35.0	37.0
9	37.476731261735786	39.0	37.0	39.0	35.0	39.0
10	37.43315956337255	39.0	37.0	39.0	35.0	39.0
11	37.294986753158014	39.0	37.0	39.0	34.0	39.0
12	37.1398296795968	39.0	37.0	39.0	34.0	39.0
13	37.08327977204073	39.0	37.0	39.0	33.0	39.0
14	38.31214319389969	40.0	38.0	41.0	34.0	41.0
15	38.44202354413195	40.0	38.0	41.0	34.0	41.0
16	38.52057339579797	40.0	38.0	41.0	34.0	41.0
17	38.50990921473586	40.0	38.0	41.0	34.0	41.0
18	38.4566784546583	40.0	37.0	41.0	34.0	41.0
19	38.4359396423578	40.0	37.0	41.0	34.0	41.0
20	38.364097497478184	40.0	37.0	41.0	34.0	41.0
21	38.30570321845525	40.0	37.0	41.0	34.0	41.0
22	38.27445797925037	40.0	37.0	41.0	34.0	41.0
23	38.20765585434694	40.0	37.0	41.0	34.0	41.0
24	38.190862698894186	40.0	37.0	41.0	34.0	41.0
25	38.12983437616344	40.0	37.0	41.0	34.0	41.0
26	38.03819092940637	40.0	36.0	41.0	34.0	41.0
27	37.96527154899258	40.0	36.0	41.0	34.0	41.0
28	37.90998818195995	40.0	36.0	41.0	34.0	41.0
29	37.87667681401506	40.0	36.0	41.0	34.0	41.0
30	37.771105027309495	40.0	36.0	41.0	33.0	41.0
31	33.1907905827078	38.0	33.0	41.0	9.0	41.0
32	34.11406977781905	38.0	33.0	41.0	16.0	41.0
33	36.059926747983674	38.0	34.0	41.0	30.0	41.0
34	36.89041404606962	38.0	35.0	41.0	31.0	41.0
35	37.04937074120115	39.0	35.0	41.0	32.0	41.0
36	37.19685104672137	39.0	35.0	41.0	33.0	41.0
37	37.26252184895085	39.0	35.0	41.0	33.0	41.0
38	37.259067483622864	39.0	35.0	41.0	33.0	41.0
39	37.2906435558328	39.0	35.0	41.0	33.0	41.0
40	37.2476767319829	39.0	35.0	41.0	33.0	41.0
41	37.157953378688404	39.0	35.0	41.0	33.0	41.0
42	37.07234876108899	39.0	35.0	41.0	32.0	41.0
43	37.0374580486622	39.0	35.0	41.0	32.0	41.0
44	36.95671045621601	39.0	35.0	41.0	32.0	41.0
45	36.931251639516425	39.0	35.0	41.0	32.0	41.0
46	36.89767885039587	39.0	35.0	41.0	32.0	41.0
47	36.80327786096179	39.0	35.0	41.0	32.0	41.0
48	36.79251091433408	39.0	35.0	41.0	32.0	41.0
49	36.73793608883272	39.0	35.0	41.0	32.0	41.0
50	36.54683180071413	39.0	35.0	40.0	31.0	41.0
51	35.91116367579808	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	3.0
10	7.0
11	10.0
12	5.0
13	8.0
14	25.0
15	41.0
16	72.0
17	113.0
18	213.0
19	408.0
20	657.0
21	1091.0
22	1783.0
23	2816.0
24	3907.0
25	5191.0
26	6337.0
27	7507.0
28	9263.0
29	11883.0
30	15758.0
31	22302.0
32	34672.0
33	40123.0
34	74071.0
35	110346.0
36	129112.0
37	129709.0
38	195075.0
39	306563.0
40	248.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.41767802105973	21.851024185064556	25.33369511620171	11.397602677674
2	31.392175934648314	25.274740133829614	25.23318318142359	18.09990075009848
3	32.102069644404104	24.392578883839754	26.18971424862596	17.315637223130185
4	29.386985372133047	25.208122806653797	26.202064145544888	19.202827675668267
5	22.863896022882464	39.78550843263582	19.950221802345762	17.400373742135955
6	27.715332171661768	34.17577058398786	22.449588532084043	15.659308712266332
7	79.15661922924023	7.024747570811334	8.180950329075173	5.637682870873264
8	80.17751399279379	5.9611239668229485	7.824516077853029	6.036845962530232
9	73.69363781989162	7.983081542673401	10.424124306670477	7.899156330764495
10	42.37177516697151	24.250870577587552	17.716242638514913	15.66111161692603
11	31.369910062101052	26.066305424669682	23.41080715140162	19.15297736182764
12	28.827544056228994	22.924924345613217	28.52871260888417	19.71881898927362
13	24.076980423159753	24.97158171530152	30.19108085035801	20.760357011180712
14	19.33353826349632	29.082294484644212	28.268463321256874	23.315703930602595
15	18.28226455642686	26.92665152827721	34.09950771688267	20.691576198413266
16	20.87394000474164	25.830305204715316	31.609966817539735	21.685787973003308
17	21.322142103142372	25.577718261891736	27.979457704307407	25.120681930658485
18	21.305104654108234	26.088210716285005	29.36372790202295	23.24295672758381
19	23.658796687342978	27.33068246251536	27.42939149263378	21.58112935750788
20	26.71300732610308	26.484579305719446	26.830556709915342	19.971856658262126
21	25.0175557841238	26.27508178426263	27.38152437391882	21.325838057694753
22	21.23848732693242	26.277605850786202	26.321686869715798	26.16221995256558
23	21.799100530865275	27.00606947853687	27.33410798136878	23.860722009229068
24	22.710378691109245	25.5707770789519	27.889853342720457	23.8289908872184
25	22.096309364016367	27.363495327321846	26.52541509626159	24.014780212400197
26	22.556320487938116	27.972786957066532	26.918358166842598	22.552534388152754
27	22.94998472038301	28.407016544354608	27.119652472097798	21.523346263164584
28	22.12849121219196	27.32455258667239	27.85352481382756	22.69343138730809
29	23.244669487010523	26.824787415004316	26.63854736365759	23.291995734327575
30	23.644914321463308	25.80704773460522	26.8141502775121	23.733887666419367
31	25.601426458166753	28.519517795119718	23.31552364013662	22.563532106576904
32	24.81860525492621	28.566393316271842	24.96211646583811	21.65288496296383
33	24.86584135701028	27.166978719414846	24.97374520089316	22.993434722681712
34	24.27863530934689	26.87031075766167	26.36973427889673	22.481319654094712
35	23.437670430831112	25.85635717704794	26.685422884809718	24.02054950731123
36	24.938047688631155	25.979675855771234	26.332324007208012	22.749952448389603
37	24.40114268097332	27.753463605214364	27.858663092107694	19.98673062170463
38	23.216183593387306	27.2149359833628	26.384878678038188	23.18400174521171
39	24.691139895485616	25.450523338150095	26.275802946126504	23.582533820237785
40	23.39539231656121	24.910643537803757	29.181183805228606	22.51278034040643
41	21.399126132111444	25.942986745946396	28.241690187060374	24.416196934881786
42	24.400421519109436	24.529239057044805	28.18742275680349	22.88291666704227
43	25.42951949886462	24.438462807429048	28.391511564281213	21.740506129425118
44	23.126849667499307	25.103464191158377	26.583017900138913	25.186668241203403
45	22.00940935941896	24.84790245564629	26.88365225214343	26.25903593279132
46	22.88625204066271	25.625945961538637	27.822695144146735	23.665106853651917
47	21.9567645433558	24.949045407055305	30.3644301333879	22.729759916200994
48	22.454907100830148	22.7725789018688	29.539601251576414	25.23291274572464
49	23.17769157890277	22.915819677081746	30.701302869052334	23.205185874963153
50	21.734556544048118	24.057148471903083	28.930129331365762	25.278165652683036
51	20.91621811901154	23.65789523501313	27.163823636260382	28.26206300971495
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	906.0
1	1197.5
2	1489.0
3	1812.0
4	2135.0
5	1844.5
6	1554.0
7	1589.5
8	1625.0
9	1709.5
10	1794.0
11	2139.0
12	2484.0
13	2479.0
14	2474.0
15	2670.0
16	2866.0
17	2747.5
18	2629.0
19	2630.0
20	2631.0
21	3611.5
22	4592.0
23	4485.0
24	4378.0
25	5109.5
26	7271.5
27	8702.0
28	10940.0
29	13178.0
30	14150.0
31	15122.0
32	17046.5
33	18971.0
34	21684.5
35	24398.0
36	26373.0
37	28348.0
38	33290.0
39	38232.0
40	42056.0
41	45880.0
42	52346.0
43	58812.0
44	68350.5
45	77889.0
46	89852.5
47	101816.0
48	109725.5
49	117635.0
50	113769.5
51	109904.0
52	96368.5
53	82833.0
54	73578.5
55	64324.0
56	59593.0
57	54862.0
58	51845.0
59	48828.0
60	47710.5
61	46593.0
62	43831.5
63	41070.0
64	33777.5
65	26485.0
66	21437.0
67	16389.0
68	13894.0
69	11399.0
70	9880.0
71	8361.0
72	6683.5
73	5006.0
74	4234.5
75	2740.5
76	2018.0
77	1462.5
78	907.0
79	584.0
80	261.0
81	175.0
82	89.0
83	89.0
84	89.0
85	63.5
86	38.0
87	29.0
88	20.0
89	10.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1109321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.84868711137175
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.4691011101414	17.262159573941517
2	13.013126853269672	6.467182350349756
3	5.324645999866877	3.9693138728852766
4	2.719172954548885	2.702715117971581
5	1.5751914147999653	1.9570719303441668
6	0.9826925305847352	1.4651171531509317
7	0.6586986577683412	1.1457457793296237
8	0.4964336329316184	0.986857921302349
9	0.3533436439224443	0.7902113085558694
>10	3.3708730015972805	20.677559080540316
>50	1.7737041507345155	31.292079242467953
>100	0.2539180103745368	8.711569010255149
>500	0.007030303218838967	1.1721954314359244
>1k	0.0020677362408349905	1.4002222274696157
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	4023	0.36265427229809943	RNA PCR Primer, Index 41 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	3769	0.3397573831199445	Illumina PCR Primer Index 3 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGCT	3627	0.32695676003609414	RNA PCR Primer, Index 41 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCGTGACATGTCGTATGCCGTCTTCTGC	1199	0.10808413434884943	Illumina PCR Primer Index 3 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	9.014523298486191E-5	0.0	0.0	0.34237159487650554	0.0
2	9.014523298486191E-5	0.0	0.0	1.2986322263799206	0.0
3	9.014523298486191E-5	0.0	0.0	1.8064203237836478	0.0
4	9.014523298486191E-5	0.0	0.0	2.4103933847822225	0.0
5	9.014523298486191E-5	0.0	0.0	3.9697256249543638	0.0
6	9.014523298486191E-5	0.0	0.0	5.0180245393353236	0.0
7	9.014523298486191E-5	0.0	0.0	5.922541807105428	0.0
8	9.014523298486191E-5	0.0	0.0	7.431302571573061	0.0
9	9.014523298486191E-5	0.0	0.0	7.988219820953538	0.0
10	9.014523298486191E-5	0.0	0.0	9.139013865238285	0.0
11	9.014523298486191E-5	0.0	0.0	10.831490614529068	0.0
12	9.014523298486191E-5	0.0	0.0	12.199805106006286	0.0
13	9.014523298486191E-5	0.0	0.0	12.749961462912898	0.0
14	9.014523298486191E-5	0.0	0.0	12.979020499927433	0.0
15	9.014523298486191E-5	0.0	0.0	13.394139297822722	0.0
16	9.014523298486191E-5	0.0	0.0	14.233481562144771	0.0
17	9.014523298486191E-5	0.0	0.0	15.186587110493717	0.0
18	9.014523298486191E-5	0.0	0.0	16.377225347757772	0.0
19	9.014523298486191E-5	0.0	0.0	17.06115723041392	0.0
20	9.014523298486191E-5	0.0	0.0	17.644937759223886	0.0
21	1.8029046596972382E-4	0.0	0.0	18.323551073133928	0.0
22	1.8029046596972382E-4	0.0	0.0	19.018931400379152	0.0
23	1.8029046596972382E-4	0.0	0.0	19.654455292922428	0.0
24	1.8029046596972382E-4	0.0	0.0	20.17675677283672	0.0
25	1.8029046596972382E-4	0.0	0.0	20.64992910077426	0.0
26	1.8029046596972382E-4	0.0	0.0	21.06360557494179	0.0
27	1.8029046596972382E-4	0.0	0.0	21.50378474760687	0.0
28	1.8029046596972382E-4	0.0	0.0	21.935309977905405	0.0
29	1.8029046596972382E-4	0.0	0.0	22.40857245107593	0.0
30	1.8029046596972382E-4	0.0	0.0	22.88381811937212	0.0
31	2.7043569895458575E-4	0.0	0.0	23.367447294335904	0.0
32	2.7043569895458575E-4	0.0	0.0	23.8891177576193	0.0
33	2.7043569895458575E-4	0.0	0.0	24.38212203681351	0.0
34	2.7043569895458575E-4	0.0	0.0	24.86439903328252	0.0
35	3.6058093193944764E-4	0.0	0.0	25.3665079810082	0.0
36	3.6058093193944764E-4	0.0	0.0	25.890522220349204	0.0
37	3.6058093193944764E-4	0.0	0.0	26.44906208392341	0.0
38	3.6058093193944764E-4	0.0	0.0	26.943508686845377	0.0
39	3.6058093193944764E-4	0.0	0.0	27.447961410628665	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTACG	30	2.1654323E-6	45.000004	1
CACGGAC	30	2.1654323E-6	45.000004	18
GTAATCG	30	2.1654323E-6	45.000004	1
AACGCGT	30	2.1654323E-6	45.000004	31
TCACGAG	25	3.8907187E-5	45.0	25
ACGTTAC	20	7.03318E-4	45.0	36
CGCATAC	25	3.8907187E-5	45.0	44
GTTAGCA	25	3.8907187E-5	45.0	11
GAATAGT	35	1.2118835E-7	45.0	17
CGTTACA	55	1.8189894E-12	45.0	28
TCGGACA	25	3.8907187E-5	45.0	21
TGCGCGA	25	3.8907187E-5	45.0	20
ATTCACG	35	1.2118835E-7	45.0	1
TACGATG	35	1.2118835E-7	45.0	1
TTAAACG	50	2.1827873E-11	45.0	1
CGGTCTA	25	3.8907187E-5	45.0	31
AACTCGT	20	7.03318E-4	45.0	22
CACGTTA	20	7.03318E-4	45.0	35
CGCGTTA	55	1.8189894E-12	45.0	26
CGACTTA	25	3.8907187E-5	45.0	34
>>END_MODULE