##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550087_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1237586
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.223398616338585	33.0	31.0	34.0	30.0	34.0
2	32.38675453665442	34.0	31.0	34.0	30.0	34.0
3	32.393276103640474	34.0	31.0	34.0	30.0	34.0
4	35.94960431032672	37.0	35.0	37.0	35.0	37.0
5	35.86593093328464	37.0	35.0	37.0	35.0	37.0
6	35.799747249888085	37.0	35.0	37.0	35.0	37.0
7	36.09825014180833	37.0	35.0	37.0	35.0	37.0
8	35.810689519758625	37.0	35.0	37.0	35.0	37.0
9	37.54416662761214	39.0	38.0	39.0	35.0	39.0
10	37.36395935312778	39.0	37.0	39.0	34.0	39.0
11	37.29476577789342	39.0	37.0	39.0	34.0	39.0
12	37.11554914163541	39.0	37.0	39.0	34.0	39.0
13	36.982716352641354	39.0	37.0	39.0	33.0	39.0
14	38.06032631267645	40.0	37.0	41.0	33.0	41.0
15	38.166949205954175	40.0	37.0	41.0	33.0	41.0
16	38.246261673936196	40.0	37.0	41.0	34.0	41.0
17	38.2740633782218	40.0	37.0	41.0	34.0	41.0
18	38.29042506944972	40.0	37.0	41.0	34.0	41.0
19	38.2619939139583	40.0	37.0	41.0	34.0	41.0
20	38.21857228507756	40.0	36.0	41.0	34.0	41.0
21	38.132856221709034	40.0	36.0	41.0	34.0	41.0
22	38.059899675658905	40.0	36.0	41.0	34.0	41.0
23	37.848865452582686	40.0	36.0	41.0	33.0	41.0
24	37.63276491492309	39.0	35.0	41.0	33.0	41.0
25	37.58709697750298	39.0	35.0	41.0	33.0	41.0
26	37.75486148033349	39.0	36.0	41.0	33.0	41.0
27	37.81542454423369	40.0	36.0	41.0	34.0	41.0
28	37.783819467899605	40.0	36.0	41.0	34.0	41.0
29	37.83516943468979	40.0	36.0	41.0	34.0	41.0
30	37.77046524443554	40.0	36.0	41.0	34.0	41.0
31	37.661227583376025	40.0	36.0	41.0	33.0	41.0
32	37.59610483635077	40.0	35.0	41.0	33.0	41.0
33	37.526676125941954	40.0	35.0	41.0	33.0	41.0
34	37.38648304037053	40.0	35.0	41.0	33.0	41.0
35	37.34282223619207	40.0	35.0	41.0	33.0	41.0
36	37.32969102753263	39.0	35.0	41.0	33.0	41.0
37	37.19042959438779	39.0	35.0	41.0	33.0	41.0
38	37.12784323675284	39.0	35.0	41.0	33.0	41.0
39	37.084504834411504	39.0	35.0	41.0	32.0	41.0
40	37.013659656783446	39.0	35.0	41.0	32.0	41.0
41	37.01946692997497	39.0	35.0	41.0	32.0	41.0
42	37.01228843894485	39.0	35.0	41.0	32.0	41.0
43	36.90273080012217	39.0	35.0	41.0	32.0	41.0
44	36.761921191739404	39.0	35.0	41.0	32.0	41.0
45	36.76707154088685	39.0	35.0	41.0	32.0	41.0
46	36.698972839059266	39.0	35.0	41.0	32.0	41.0
47	36.65619682187743	39.0	35.0	41.0	31.0	41.0
48	36.62743276022838	39.0	35.0	41.0	32.0	41.0
49	36.558555122633905	39.0	35.0	40.0	31.0	41.0
50	36.471772466721504	39.0	35.0	40.0	31.0	41.0
51	35.459123648780775	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	13.0
10	19.0
11	21.0
12	24.0
13	13.0
14	20.0
15	39.0
16	66.0
17	162.0
18	283.0
19	513.0
20	892.0
21	1441.0
22	2258.0
23	3295.0
24	4661.0
25	5716.0
26	6975.0
27	8435.0
28	10185.0
29	12480.0
30	15846.0
31	20453.0
32	27772.0
33	39941.0
34	79611.0
35	118968.0
36	86719.0
37	136480.0
38	258616.0
39	395280.0
40	383.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94398288280572	23.1819041262587	25.21497495931596	10.659138031619621
2	30.043730294298737	24.812497878935282	26.151313928890595	18.992457897875383
3	29.80778709519985	25.277031252777583	27.366421404249884	17.54876024777268
4	28.335566174795122	25.901068693407975	25.860667460685562	19.90269767111134
5	24.925459725627146	30.26658349399557	25.238730884156734	19.569225896220548
6	25.852587214141074	33.7851268517905	24.37697259018767	15.98531334388075
7	79.37961483080772	5.940516457038137	8.981274836657816	5.6985938754963295
8	78.18357673729341	5.455378454507405	8.774824537446287	7.586220270752901
9	72.34325533740686	7.597855825776955	12.130712532300786	7.928176304515404
10	39.93209360804017	24.879725530185375	19.194625666418332	15.993555195356121
11	30.654273723199843	24.495267399598898	24.434261538188053	20.41619733901321
12	27.730759720940608	21.810605485194564	29.808029502596185	20.650605291268647
13	22.92988123653629	26.330937809574444	30.4362686714297	20.302912282459562
14	17.442747413109068	30.567249467915765	29.55212809453242	22.437875024442747
15	16.616461401470282	25.812994006073115	37.418732920378865	20.151811672077738
16	18.220794352877295	24.450745241138797	33.318007799053966	24.010452606929945
17	18.88878833470967	24.539062335869993	28.178647786901273	28.39350154251907
18	19.690510396853227	26.33457392051946	31.506416523780974	22.468499158846335
19	22.479165084285054	27.388157267454545	27.046443641088374	23.086234007172028
20	24.06467106124342	27.528187940070424	26.89720148741178	21.509939511274368
21	21.550421546462225	28.35116105062598	28.404409875354116	21.694007527557684
22	19.73794144406934	26.49625965387456	26.113579177527864	27.652219724528237
23	19.29231584714113	27.63226151556336	27.355432268949393	25.719990368346117
24	21.22882773399182	24.559343754696645	30.411704721934473	23.80012378937706
25	19.423215841161745	28.089037852722964	26.83563000874283	25.65211629737247
26	18.06129028608921	29.07959527661108	27.87143681328005	24.987677624019664
27	20.306063578611912	27.490776398569473	28.007588967554575	24.195571055264036
28	18.089490346529452	27.390338934021557	30.120977451263993	24.399193268184998
29	19.39396534867072	25.222651193533217	28.483838698886384	26.899544758909684
30	21.050092680427866	26.23260120912809	29.08460502946866	23.632701080975384
31	22.84002889496164	28.950715344226584	24.063378221796302	24.14587753901547
32	23.05989240343701	28.153356615217046	23.523052135366754	25.26369884597919
33	23.517395962785617	26.39162046112351	27.342261467081887	22.74872210900899
34	20.813179851743634	26.48720977774474	26.867466180128087	25.832144190383538
35	19.55371182285514	25.475078095582855	26.840639761600404	28.1305703199616
36	23.858463169428227	25.850163140177735	27.725345955755802	22.56602773463824
37	22.292834598969282	28.311648645023457	28.56585320131288	20.82966355469438
38	20.8450160231289	28.506140179349153	26.1934928158528	24.455350981669152
39	23.45630929890933	26.071642697961998	28.427761787867674	22.044286215261
40	21.86563196416249	24.481126968146054	29.49289988736136	24.160341180330093
41	19.986893840104848	24.588998259514895	26.37343990639842	29.050667993981833
42	23.01262296115179	23.498245778475194	26.522601257609573	26.966530002763445
43	24.82162855753055	23.332358316916967	28.227694883426285	23.618318242126204
44	21.85722850775623	24.77662158427778	27.0749669113904	26.29118299657559
45	20.52423023531294	25.379731186357958	27.35422023196772	26.741818346361384
46	22.24912046516363	25.17416971426632	28.147215627843238	24.429494192726807
47	19.408186582589007	25.079307619834097	30.90322611923535	24.609279678341544
48	20.564146653242684	21.830402089228546	29.963816655973808	27.641634601554966
49	22.163550654257563	21.087908234256044	32.153078654735914	24.59546245675048
50	20.087654514514547	22.430845209949048	31.500437141338054	25.98106313419835
51	19.45981935800825	22.647315014875737	26.96798444714145	30.924881179974562
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1024.0
1	1237.0
2	1450.0
3	1898.5
4	2347.0
5	1914.5
6	1482.0
7	1486.0
8	1490.0
9	1662.0
10	1834.0
11	1901.0
12	1968.0
13	2017.5
14	2067.0
15	2273.0
16	2479.0
17	2499.5
18	2520.0
19	2604.0
20	2688.0
21	2915.5
22	3143.0
23	3453.0
24	3763.0
25	4551.0
26	7202.5
27	9066.0
28	10897.0
29	12728.0
30	14523.0
31	16318.0
32	17503.0
33	18688.0
34	23649.5
35	28611.0
36	31273.5
37	33936.0
38	36302.0
39	38668.0
40	47091.5
41	55515.0
42	64410.0
43	73305.0
44	82365.0
45	91425.0
46	108526.0
47	125627.0
48	143352.5
49	161078.0
50	155261.5
51	149445.0
52	125482.0
53	101519.0
54	84294.5
55	67070.0
56	57268.0
57	47466.0
58	44049.5
59	40633.0
60	39869.0
61	39105.0
62	34823.0
63	30541.0
64	25552.5
65	20564.0
66	17534.5
67	14505.0
68	12115.5
69	9726.0
70	7833.0
71	5940.0
72	5660.5
73	5381.0
74	4204.0
75	2735.5
76	2444.0
77	1708.0
78	972.0
79	705.0
80	438.0
81	287.5
82	137.0
83	108.0
84	79.0
85	46.0
86	13.0
87	10.0
88	7.0
89	6.5
90	6.0
91	5.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	1.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1237586.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.122354253014223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.12746723902045	13.105179673851477
2	12.348046791204824	4.96943543730838
3	6.016906170290654	3.6322295239720708
4	3.4254404227311257	2.7571170263516205
5	2.310476071543633	2.324610900235681
6	1.632313382568609	1.9707592881588891
7	1.192783706433327	1.6801131401652312
8	0.9178537771155019	1.4775503084468231
9	0.6839437774304968	1.2386303080741057
>10	4.216730515755672	16.5737485239588
>50	1.0737658220202717	15.995084130645493
>100	1.0376216438856896	30.114618389135916
>500	0.012183424390047192	1.6017883727906401
>1k	0.003655027317014158	1.4129340832381088
>5k	8.122282926698128E-4	1.1462008936667272
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	8088	0.6535303405177498	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	5938	0.47980503981137473	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	4458	0.36021739095303273	No Hit
TCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	2787	0.2251964711947291	No Hit
ACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	2184	0.17647258453149922	No Hit
GCCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	1563	0.1262942534902625	No Hit
ACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	1494	0.12071888337456951	No Hit
AGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTG	1329	0.1073864765761733	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1315	0.10625524205994574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3811452295032426	0.0
2	0.0	0.0	0.0	2.006406019460466	0.0
3	0.0	0.0	0.0	2.8292983275505703	0.0
4	0.0	0.0	0.0	3.7863227282790852	0.0
5	0.0	0.0	0.0	6.310026131517325	0.0
6	0.0	0.0	0.0	7.715989030257291	0.0
7	0.0	0.0	0.0	9.106276250700962	0.0
8	0.0	0.0	0.0	11.412136207099952	0.0
9	0.0	0.0	0.0	12.229695552470696	0.0
10	0.0	0.0	0.0	14.035388247766216	0.0
11	0.0	0.0	0.0	16.532911652200333	0.0
12	0.0	0.0	0.0	18.338119532703182	0.0
13	0.0	0.0	0.0	19.0718059189422	0.0
14	0.0	0.0	0.0	19.36269479454357	0.0
15	0.0	0.0	0.0	19.907303411641696	0.0
16	0.0	0.0	0.0	21.01163070687613	0.0
17	0.0	0.0	0.0	22.346891448351872	0.0
18	0.0	0.0	0.0	23.898783599685192	0.0
19	0.0	0.0	0.0	24.689031711735588	0.0
20	0.0	0.0	0.0	25.492531428118934	0.0
21	0.0	0.0	0.0	26.452626322534353	0.0
22	0.0	0.0	0.0	27.455627326100974	0.0
23	0.0	0.0	0.0	28.421459195562974	0.0
24	0.0	0.0	0.0	29.202657431483548	0.0
25	0.0	0.0	0.0	29.859096660757313	0.0
26	0.0	0.0	0.0	30.424633116405648	0.0
27	0.0	0.0	0.0	30.964555190507973	0.0
28	0.0	0.0	0.0	31.567503187657262	0.0
29	0.0	0.0	0.0	32.18952056665153	0.0
30	0.0	0.0	0.0	32.9770213948768	0.0
31	0.0	0.0	0.0	33.59960439112918	0.0
32	0.0	0.0	0.0	34.19091683325442	0.0
33	0.0	0.0	0.0	34.761705449156665	0.0
34	0.0	0.0	0.0	35.31633357196995	0.0
35	0.0	0.0	0.0	35.96420773990656	0.0
36	8.080246544482566E-5	0.0	0.0	36.53839005935749	0.0
37	8.080246544482566E-5	0.0	0.0	37.077423306339924	0.0
38	8.080246544482566E-5	0.0	0.0	37.643282971850034	0.0
39	8.080246544482566E-5	0.0	0.0	38.189507638257055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGCGAG	50	2.1827873E-11	45.000004	1
CTTCGAC	35	1.212029E-7	45.000004	25
AACCGGT	25	3.890979E-5	45.000004	11
GGTACTC	25	3.890979E-5	45.000004	8
ATCTCGA	30	2.1656379E-6	45.000004	21
TCGCACC	35	1.212029E-7	45.000004	39
AGATCGG	45	3.8562575E-10	45.000004	2
GTCGATA	25	3.890979E-5	45.000004	1
CGACGGT	55	1.8189894E-12	45.000004	28
TAGAACG	30	2.1656379E-6	45.000004	1
GACCGAA	35	1.212029E-7	45.000004	9
ATTCGGA	35	1.212029E-7	45.000004	12
CGATTTT	50	2.1827873E-11	45.000004	37
CGTTCAA	60	0.0	45.000004	9
ATCGTGC	25	3.890979E-5	45.000004	38
CGTTAGC	35	1.212029E-7	45.000004	11
ATTCCGT	25	3.890979E-5	45.000004	28
CCCGTAT	35	1.212029E-7	45.000004	42
TATTCCG	25	3.890979E-5	45.000004	27
GTCACGA	25	3.890979E-5	45.000004	23
>>END_MODULE