SRR3550085_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769799
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	5593	0.7265532950809237	TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	5187	0.6738122548873148	TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	3882	0.5042874828364287	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	2088	0.2712396352814176	No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2082	0.2704602110421032	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	2009	0.26097721613044444	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1980	0.25720999897375807	TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1750	0.22733206980003873	Illumina Single End Adapter 2 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1097	0.14250473175465284	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	1021	0.13263202472333688	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	1016	0.1319825045239082	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	1016	0.1319825045239082	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC	945	0.1227593176920209	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	858	0.11145766622196182	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	812	0.10548208038721797	No Hit
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	790	0.10262419150973176	TruSeq Adapter, Index 22 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	7.0318324E-4	45.000004	33
AGCCGAC	20	7.0318324E-4	45.000004	42
GTCGCGA	20	7.0318324E-4	45.000004	19
TCCATAT	45	3.8380676E-10	45.000004	28
ACGCCCA	20	7.0318324E-4	45.000004	17
GTATTCG	20	7.0318324E-4	45.000004	1
CGAAACG	40	6.8102963E-9	45.000004	8
CTATCGG	20	7.0318324E-4	45.000004	2
CTTCATA	20	7.0318324E-4	45.000004	26
GTCCTAA	35	1.2112287E-7	45.000004	39
TAGGTGT	20	7.0318324E-4	45.000004	5
CACATCG	20	7.0318324E-4	45.000004	10
TAGCGGT	20	7.0318324E-4	45.000004	39
CGGCACA	20	7.0318324E-4	45.000004	42
TTATCGG	35	1.2112287E-7	45.000004	2
TAGCCCG	20	7.0318324E-4	45.000004	1
CCAGACG	45	3.8380676E-10	45.000004	1
TGTTGCG	40	6.8102963E-9	45.000004	1
ACGATTG	20	7.0318324E-4	45.000004	1
GTTACGA	20	7.0318324E-4	45.000004	18

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content