##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550085_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769799
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.208394658865494	33.0	31.0	34.0	30.0	34.0
2	32.383609227863374	34.0	31.0	34.0	30.0	34.0
3	32.39973551537479	34.0	31.0	34.0	30.0	34.0
4	35.93797082095456	37.0	35.0	37.0	35.0	37.0
5	35.848368210402974	37.0	35.0	37.0	35.0	37.0
6	35.79663652459928	37.0	35.0	37.0	35.0	37.0
7	36.06097825536276	37.0	35.0	37.0	35.0	37.0
8	35.741335075779524	37.0	35.0	37.0	35.0	37.0
9	37.50675955671545	39.0	37.0	39.0	35.0	39.0
10	37.38833903395562	39.0	37.0	39.0	34.0	39.0
11	37.32654238314157	39.0	37.0	39.0	34.0	39.0
12	37.13101212134596	39.0	37.0	39.0	34.0	39.0
13	36.9789659378617	39.0	37.0	39.0	33.0	39.0
14	38.01526372468658	40.0	37.0	41.0	33.0	41.0
15	38.11281516343877	40.0	37.0	41.0	33.0	41.0
16	38.228271276008414	40.0	37.0	41.0	34.0	41.0
17	38.24894680299663	40.0	37.0	41.0	34.0	41.0
18	38.27470417602517	40.0	37.0	41.0	34.0	41.0
19	38.25149681929958	40.0	37.0	41.0	34.0	41.0
20	38.19456507477926	40.0	36.0	41.0	34.0	41.0
21	38.14124985872936	40.0	36.0	41.0	34.0	41.0
22	38.047561766123366	40.0	36.0	41.0	34.0	41.0
23	37.83175608178239	40.0	36.0	41.0	33.0	41.0
24	37.62113356863285	39.0	35.0	41.0	33.0	41.0
25	37.59695842681012	39.0	35.0	41.0	33.0	41.0
26	37.76499319952351	39.0	36.0	41.0	33.0	41.0
27	37.78819016392591	40.0	36.0	41.0	34.0	41.0
28	37.78827719963263	40.0	36.0	41.0	34.0	41.0
29	37.85026610842571	40.0	36.0	41.0	34.0	41.0
30	37.74232364552305	40.0	36.0	41.0	34.0	41.0
31	37.5738965626092	40.0	36.0	41.0	33.0	41.0
32	37.53326647605414	40.0	35.0	41.0	33.0	41.0
33	37.472276529327786	40.0	35.0	41.0	33.0	41.0
34	37.31409367899932	39.0	35.0	41.0	33.0	41.0
35	37.289528825056934	39.0	35.0	41.0	33.0	41.0
36	37.20883633260111	39.0	35.0	41.0	33.0	41.0
37	37.08422198521952	39.0	35.0	41.0	32.0	41.0
38	37.052221424034066	39.0	35.0	41.0	32.0	41.0
39	37.0276227950413	39.0	35.0	41.0	32.0	41.0
40	36.926831549534356	39.0	35.0	41.0	32.0	41.0
41	36.84381637284538	39.0	35.0	41.0	32.0	41.0
42	36.873246133081494	39.0	35.0	41.0	32.0	41.0
43	36.811133815450525	39.0	35.0	41.0	32.0	41.0
44	36.668387462181684	39.0	35.0	41.0	31.0	41.0
45	36.66628301673554	39.0	35.0	41.0	31.0	41.0
46	36.51783127803492	39.0	35.0	40.0	31.0	41.0
47	36.420275942161524	39.0	35.0	40.0	31.0	41.0
48	36.4132572268865	39.0	35.0	40.0	31.0	41.0
49	36.38808961819904	39.0	35.0	40.0	31.0	41.0
50	36.31954055539173	39.0	35.0	40.0	31.0	41.0
51	35.19227876367727	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	11.0
10	14.0
11	13.0
12	7.0
13	5.0
14	12.0
15	22.0
16	44.0
17	90.0
18	181.0
19	326.0
20	639.0
21	1018.0
22	1638.0
23	2329.0
24	3118.0
25	3803.0
26	4589.0
27	5278.0
28	6274.0
29	7608.0
30	9861.0
31	12817.0
32	17555.0
33	25829.0
34	50450.0
35	71426.0
36	55472.0
37	89537.0
38	166240.0
39	233422.0
40	169.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.299871784712636	22.728140722448327	24.833495496876456	11.138491995962582
2	30.089153142573583	26.33752447067351	24.849733501862175	18.72358888489073
3	30.989907755141278	25.455086327729703	26.777769261846274	16.777236655282742
4	28.717886097539747	26.146825340121254	26.641889636125793	18.493398926213207
5	24.487431134620856	29.947038122938586	24.590445038250245	20.975085704190313
6	25.10915186951399	34.228025757373025	24.273868893048707	16.388953480064277
7	76.30056677132602	7.451035919766069	10.319317120443129	5.9290801884647815
8	74.6752074242757	6.839837412103679	10.483775634938471	8.001179528682162
9	69.04477662350821	8.056258841593715	13.330882477114155	9.568082057783915
10	39.25609152519034	25.9323537702699	19.48560598286046	15.325948721679294
11	30.644752721164874	25.408970393570268	24.249446933550185	19.69682995171467
12	29.36558764041003	20.8777875783159	30.1099377889553	19.646686992318774
13	24.785431002118735	25.679430604612374	31.4760086723937	18.059129720875188
14	17.866092317604984	30.750234801552097	28.55030988608715	22.833362994755774
15	15.623169164937861	26.381432036154894	38.488488553505526	19.50691024540172
16	17.91740441335985	25.631366109854653	32.69710664731962	23.754122829465874
17	17.33582402679141	25.859087891774347	28.929889490633272	27.875198590800977
18	18.540164380572072	27.40728423913255	31.802717332706333	22.249834047589047
19	20.899221744897044	27.912481050248182	27.663065293667565	23.525231911187205
20	23.032246079820837	27.641761031126304	28.942100470382528	20.383892418670328
21	21.5571857069183	28.310247220378304	28.98886592474139	21.143701147962002
22	17.82880985815778	27.089668861611926	27.45210113289313	27.629420147337164
23	18.550296895683157	28.52471879022966	28.26075378118184	24.664230532905343
24	21.990545583977116	25.51873930727372	28.2775114023271	24.213203706422064
25	18.925719570952936	28.060701559757806	27.502503900368797	25.51107496892046
26	18.490930749455377	28.504323855967595	28.916639278564926	24.0881061160121
27	21.93001030139036	29.512639013560683	27.40429644621518	21.153054238833775
28	17.214363749498247	28.991853717658767	29.091490116251123	24.702292416591863
29	20.337516676431118	24.56433432623321	29.807910896220964	25.29023810111471
30	25.54186222637338	26.87753556447852	25.895720831022125	21.68488137812598
31	21.37155283392158	30.63046327677744	24.162151418746973	23.835832470554003
32	24.745030845714272	26.881822397794746	23.878960611796067	24.49418614469491
33	27.29920407794762	25.456645176208337	22.522892339428864	24.72125840641518
34	20.48989411521709	25.358048009935064	29.51601651859771	24.63604135625014
35	23.351420305820092	22.384804345030325	27.830511601080282	26.4332637480693
36	25.78919951831582	24.770102325412218	26.481977763026453	22.95872039324551
37	22.57225587458544	26.441057990462447	29.06447007595489	21.92221605899722
38	22.657343020710602	26.642669060365108	24.191899443880803	26.508088475043483
39	23.92299808131733	22.195664062956695	28.496399709534565	25.38493814619141
40	25.9378097399451	20.600312549119966	31.44807930381827	22.013798407116663
41	21.773995549487594	23.04588600400884	27.780758353804046	27.39936009269952
42	22.9806741759862	22.827777121040686	28.195541953159204	25.996006749813912
43	24.627207881537906	22.225801800210185	27.28803233051745	25.85895798773446
44	21.773086221208395	23.89260053598407	27.44196861778204	26.892344625025494
45	22.303874128181512	21.239830137477444	27.05511438700232	29.401181347338724
46	24.83193664839783	23.68293541560849	28.224900266173375	23.260227669820306
47	20.285944772596483	22.54705449084761	33.18307766053217	23.983923076023743
48	21.624346095539227	20.90428800245259	29.9450895623403	27.526276339667888
49	22.25126299202779	18.64655578923849	32.896769156624	26.20541206210972
50	21.313096015973002	20.487296034419376	30.596298514287497	27.603309435320128
51	19.82348639060326	20.567187018949102	26.55004747992658	33.059279110521054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1053.0
1	1167.5
2	1282.0
3	1546.0
4	1810.0
5	1448.0
6	1086.0
7	1177.0
8	1268.0
9	1359.5
10	1451.0
11	1545.0
12	1639.0
13	1795.0
14	1951.0
15	1963.5
16	1976.0
17	1909.5
18	1843.0
19	2181.0
20	2519.0
21	2786.0
22	3053.0
23	3337.5
24	3622.0
25	4648.0
26	6313.5
27	6953.0
28	6779.5
29	6606.0
30	7442.0
31	8278.0
32	10082.5
33	11887.0
34	13606.5
35	15326.0
36	16605.0
37	17884.0
38	18691.0
39	19498.0
40	24324.5
41	29151.0
42	33856.0
43	38561.0
44	42074.5
45	45588.0
46	51786.0
47	57984.0
48	72175.0
49	86366.0
50	93549.0
51	100732.0
52	97996.5
53	95261.0
54	76565.5
55	57870.0
56	48062.0
57	38254.0
58	32917.5
59	27581.0
60	25921.5
61	24262.0
62	21589.0
63	18916.0
64	15528.0
65	12140.0
66	9978.5
67	7817.0
68	5796.5
69	3776.0
70	3788.5
71	3801.0
72	3012.0
73	2223.0
74	1756.5
75	1002.0
76	714.0
77	519.0
78	324.0
79	287.0
80	250.0
81	171.5
82	93.0
83	129.5
84	166.0
85	89.5
86	13.0
87	8.5
88	4.0
89	2.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	769799.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.185842490933712
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.07462042183464	14.21549436345188
2	13.031021889540359	5.7820839827450365
3	6.278588925664194	4.178873549103195
4	3.7538933023577794	3.3313314213552285
5	2.3195249037954553	2.573030708470207
6	1.6236038026023432	2.161261093932997
7	1.1467689351317627	1.780942447783035
8	0.9024398133537415	1.6017110045290983
9	0.6932741299123911	1.3842783584347884
>10	4.323312204443088	19.214650000410487
>50	1.2303693787180925	19.89723963320055
>100	0.6066034695459548	18.787774498548654
>500	0.008877123944574948	1.3203291590602284
>1k	0.005918082629716632	2.3556111219868296
>5k	0.0011836165259433264	1.4153886569877945
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	5593	0.7265532950809237	TruSeq Adapter, Index 22 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	5187	0.6738122548873148	TruSeq Adapter, Index 22 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGCT	3882	0.5042874828364287	Illumina Single End Adapter 2 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGC	2088	0.2712396352814176	No Hit
TCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	2082	0.2704602110421032	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCG	2009	0.26097721613044444	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTC	1980	0.25720999897375807	TruSeq Adapter, Index 22 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTGC	1750	0.22733206980003873	Illumina Single End Adapter 2 (95% over 22bp)
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	1097	0.14250473175465284	No Hit
ACCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	1021	0.13263202472333688	Illumina Single End Adapter 2 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	1016	0.1319825045239082	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	1016	0.1319825045239082	TruSeq Adapter, Index 22 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCC	945	0.1227593176920209	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	858	0.11145766622196182	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	812	0.10548208038721797	No Hit
GGCTGTCTCTTATACACATCTGACGCTGACGTGCTCGTATGCCGTCTTCTG	790	0.10262419150973176	TruSeq Adapter, Index 22 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2990403988573642E-4	0.0	0.0	0.5497538967964365	0.0
2	2.5980807977147284E-4	0.0	0.0	2.6254905501306185	0.0
3	2.5980807977147284E-4	0.0	0.0	3.598731616954556	0.0
4	2.5980807977147284E-4	0.0	0.0	4.719024056929147	0.0
5	2.5980807977147284E-4	0.0	0.0	7.895047928095516	0.0
6	2.5980807977147284E-4	0.0	0.0	9.41739337151646	0.0
7	2.5980807977147284E-4	0.0	0.0	11.005859971239246	0.0
8	2.5980807977147284E-4	0.0	0.0	13.36582666384342	0.0
9	2.5980807977147284E-4	0.0	0.0	14.159410443505383	0.0
10	2.5980807977147284E-4	0.0	0.0	16.346085146901984	0.0
11	2.5980807977147284E-4	0.0	0.0	19.301791766422145	0.0
12	2.5980807977147284E-4	0.0	0.0	21.80880983217697	0.0
13	2.5980807977147284E-4	0.0	0.0	22.63512942989014	0.0
14	2.5980807977147284E-4	0.0	0.0	22.978076095188484	0.0
15	2.5980807977147284E-4	0.0	0.0	23.637469001648483	0.0
16	2.5980807977147284E-4	0.0	0.0	24.928715158112702	0.0
17	2.5980807977147284E-4	0.0	0.0	26.463011773203135	0.0
18	2.5980807977147284E-4	0.0	0.0	28.20593427635006	0.0
19	2.5980807977147284E-4	0.0	0.0	29.181123903772285	0.0
20	2.5980807977147284E-4	0.0	0.0	30.09499882436844	0.0
21	2.5980807977147284E-4	0.0	0.0	31.177878900855937	0.0
22	2.5980807977147284E-4	0.0	0.0	32.34324804267088	0.0
23	2.5980807977147284E-4	0.0	0.0	33.34909502350614	0.0
24	2.5980807977147284E-4	0.0	0.0	34.12163434870661	0.0
25	2.5980807977147284E-4	0.0	0.0	34.833768295360215	0.0
26	2.5980807977147284E-4	0.0	0.0	35.45237133329609	0.0
27	2.5980807977147284E-4	0.0	0.0	36.07331264394991	0.0
28	2.5980807977147284E-4	0.0	0.0	36.704776181834475	0.0
29	3.8971211965720923E-4	0.0	0.0	37.365078416573674	0.0
30	3.8971211965720923E-4	0.0	0.0	38.098646529808434	0.0
31	3.8971211965720923E-4	0.0	0.0	38.7692111836986	0.0
32	3.8971211965720923E-4	0.0	0.0	39.42626581744066	0.0
33	3.8971211965720923E-4	0.0	0.0	39.99927253737664	0.0
34	3.8971211965720923E-4	0.0	0.0	40.57591657042942	0.0
35	3.8971211965720923E-4	0.0	0.0	41.16074455799501	0.0
36	3.8971211965720923E-4	0.0	0.0	41.73556993448939	0.0
37	3.8971211965720923E-4	0.0	0.0	42.336895735120464	0.0
38	5.196161595429457E-4	0.0	0.0	42.9413392327088	0.0
39	5.196161595429457E-4	0.0	0.0	43.554226492889704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGGTA	20	7.0318324E-4	45.000004	33
AGCCGAC	20	7.0318324E-4	45.000004	42
GTCGCGA	20	7.0318324E-4	45.000004	19
TCCATAT	45	3.8380676E-10	45.000004	28
ACGCCCA	20	7.0318324E-4	45.000004	17
GTATTCG	20	7.0318324E-4	45.000004	1
CGAAACG	40	6.8102963E-9	45.000004	8
CTATCGG	20	7.0318324E-4	45.000004	2
CTTCATA	20	7.0318324E-4	45.000004	26
GTCCTAA	35	1.2112287E-7	45.000004	39
TAGGTGT	20	7.0318324E-4	45.000004	5
CACATCG	20	7.0318324E-4	45.000004	10
TAGCGGT	20	7.0318324E-4	45.000004	39
CGGCACA	20	7.0318324E-4	45.000004	42
TTATCGG	35	1.2112287E-7	45.000004	2
TAGCCCG	20	7.0318324E-4	45.000004	1
CCAGACG	45	3.8380676E-10	45.000004	1
TGTTGCG	40	6.8102963E-9	45.000004	1
ACGATTG	20	7.0318324E-4	45.000004	1
GTTACGA	20	7.0318324E-4	45.000004	18
>>END_MODULE