FastQCFastQC Report
Sat 18 Jun 2016
SRR3550080_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550080_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1380022
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC94730.686438332142531TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC89130.6458592689102057TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT60590.43905097165117657TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC38040.27564777952815245TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC30760.22289499732612958TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG19340.14014269337735197No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG16810.12180965230989071TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG16760.12144733924531637TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG15410.11166488650180939TruSeq Adapter, Index 14 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG15150.10978085856602286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC750.045.025
ACTATCG207.0337794E-445.028
ACGCATT207.0337794E-445.022
CTATCGA207.0337794E-445.029
ATATGCG406.8175723E-945.01
TACGGAT351.2121745E-745.030
ATGTCGC207.0337794E-445.035
AGGTTAC207.0337794E-445.015
CGTAGGA207.0337794E-445.019
CACTACG207.0337794E-445.013
ACGGATG453.8562575E-1045.012
CGAATGT207.0337794E-445.012
TTACGTC207.0337794E-445.043
TTACGAG453.8562575E-1045.01
TACTACG207.0337794E-445.01
AATAGCG351.2121745E-745.01
CGCGTAG207.0337794E-445.013
TATCGGA207.0337794E-445.028
TTCGACA207.0337794E-445.044
ATTCTCG406.8175723E-945.012
Produced by FastQC (version 0.11.5)