##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550080_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1380022
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.191883172876956	33.0	31.0	34.0	30.0	34.0
2	32.35849283562146	34.0	31.0	34.0	30.0	34.0
3	32.37291217096539	34.0	31.0	34.0	30.0	34.0
4	35.92211790826523	37.0	35.0	37.0	35.0	37.0
5	35.84733939024161	37.0	35.0	37.0	35.0	37.0
6	35.779504239787485	37.0	35.0	37.0	35.0	37.0
7	36.080527701732294	37.0	35.0	37.0	35.0	37.0
8	35.86247900395791	37.0	35.0	37.0	35.0	37.0
9	37.54628404474711	39.0	37.0	39.0	35.0	39.0
10	37.3763106675111	39.0	37.0	39.0	34.0	39.0
11	37.33246281581018	39.0	37.0	39.0	34.0	39.0
12	37.166764008110015	39.0	37.0	39.0	34.0	39.0
13	37.04396669038609	39.0	37.0	39.0	33.0	39.0
14	38.11841188039031	40.0	37.0	41.0	33.0	41.0
15	38.20336270001493	40.0	37.0	41.0	34.0	41.0
16	38.256169829176635	40.0	37.0	41.0	34.0	41.0
17	38.27223696433825	40.0	37.0	41.0	34.0	41.0
18	38.266843572058995	40.0	37.0	41.0	34.0	41.0
19	38.235886819195635	40.0	37.0	41.0	34.0	41.0
20	38.1908353634942	40.0	37.0	41.0	34.0	41.0
21	38.12974285917181	40.0	37.0	41.0	34.0	41.0
22	38.06543953647116	40.0	36.0	41.0	34.0	41.0
23	37.83622435004659	40.0	36.0	41.0	33.0	41.0
24	37.60798161188735	39.0	35.0	41.0	33.0	41.0
25	37.53736752022794	39.0	35.0	41.0	33.0	41.0
26	37.65980759727019	39.0	36.0	41.0	33.0	41.0
27	37.73665637214479	39.0	36.0	41.0	33.0	41.0
28	37.70804595868761	39.0	36.0	41.0	33.0	41.0
29	37.68865206496708	39.0	36.0	41.0	33.0	41.0
30	37.638777497750034	39.0	36.0	41.0	33.0	41.0
31	37.528918379562064	39.0	36.0	41.0	33.0	41.0
32	37.38646050570208	39.0	35.0	41.0	33.0	41.0
33	37.39106695400508	39.0	35.0	41.0	33.0	41.0
34	37.25015978006148	39.0	35.0	41.0	33.0	41.0
35	37.06805253829287	39.0	35.0	41.0	32.0	41.0
36	37.030138649963554	39.0	35.0	41.0	32.0	41.0
37	37.05262090024652	39.0	35.0	41.0	32.0	41.0
38	36.986919773742734	39.0	35.0	41.0	32.0	41.0
39	36.97148523719187	39.0	35.0	41.0	32.0	41.0
40	36.87195566447492	39.0	35.0	41.0	32.0	41.0
41	36.762085676895005	39.0	35.0	41.0	31.0	41.0
42	36.743404090659425	39.0	35.0	41.0	31.0	41.0
43	36.697693225180466	39.0	35.0	41.0	31.0	41.0
44	36.58897104538913	39.0	35.0	41.0	31.0	41.0
45	36.578598746976496	39.0	35.0	40.0	31.0	41.0
46	36.44255308973335	39.0	35.0	40.0	31.0	41.0
47	36.352609596078906	38.0	35.0	40.0	31.0	41.0
48	36.31923476582257	38.0	35.0	40.0	31.0	41.0
49	36.29828002741985	38.0	35.0	40.0	31.0	41.0
50	36.23777809339271	38.0	35.0	40.0	31.0	41.0
51	35.17547328955625	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	15.0
10	21.0
11	12.0
12	20.0
13	29.0
14	26.0
15	46.0
16	86.0
17	175.0
18	296.0
19	527.0
20	901.0
21	1531.0
22	2522.0
23	3666.0
24	5477.0
25	6761.0
26	8175.0
27	9630.0
28	11823.0
29	14648.0
30	18609.0
31	24183.0
32	32905.0
33	47592.0
34	91905.0
35	121409.0
36	105446.0
37	169114.0
38	312168.0
39	390043.0
40	251.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.74817502909374	22.27276086902962	24.04280511470107	11.936258987175567
2	31.107330172997244	24.368814410205054	25.24561202647494	19.278243390322764
3	31.04030225605099	24.984964007820164	26.79812350817596	17.17661022795289
4	28.482154632317457	27.019424328017962	25.452565248959797	19.045855790704785
5	24.798517704790214	29.055841138764453	25.256698806250917	20.888942350194416
6	25.60278024553232	33.58518922162111	24.625259597310766	16.18677093553581
7	77.725862341325	6.39504297757572	9.804698765671851	6.074395915427435
8	77.11116199596817	4.48616036555939	10.10440413268774	8.29827350578469
9	70.67677181957968	7.579299460443384	13.301309689265823	8.442619030711104
10	38.04511812130531	25.900384196773672	19.642005707155395	16.41249197476562
11	30.178649325880315	23.221151546859396	25.242061358442115	21.358137768818178
12	27.087104408480446	20.51119474906922	31.402832708464068	20.99886813398627
13	23.57781252762637	24.563593913720215	31.791956939816902	20.06663661883651
14	18.999986956729675	27.8175275466623	30.69393096631793	22.4885545302901
15	17.579792206211206	25.231771667408196	35.60667873410714	21.581757392273456
16	19.218968972958404	25.390682177530504	31.767174726200015	23.623174123311077
17	19.555557809947956	25.051702074314758	28.19020276488346	27.202537350853827
18	21.152126560301213	24.390263343627854	31.902824737576648	22.554785358494282
19	20.941042968880204	26.956236929556194	28.474401132735565	23.628318968828033
20	22.643479596702083	27.67905149338199	28.3346932150357	21.342775694880224
21	21.394731388340187	27.762818273911577	30.273647811411703	20.568802526336537
22	20.02163733621638	25.43778287592517	27.248333722216024	27.292246065642434
23	18.670064680128288	26.784138223883385	28.373025937267666	26.17277115872066
24	22.026895223409483	24.10903594290526	29.69633817431896	24.1677306593663
25	19.67396171945085	26.929135912326036	27.43891039418212	25.957991974040993
26	18.538255187236146	28.778961494816752	27.58825583939966	25.094527478547445
27	20.21685161540903	26.40798480024231	27.512967184581115	25.86219639976754
28	16.784949805148035	26.094294148933855	28.810192880983053	28.310563164935054
29	18.260216141481802	25.568432967010672	29.088159464124487	27.083191427383042
30	21.13422829491124	24.774749967754136	26.115598157130826	27.975423580203795
31	21.74247946771863	27.22282688246999	23.423539624730623	27.611154025080758
32	20.98336113482249	26.889716250900346	26.77210943013952	25.354813184137644
33	21.18690861450035	23.342019185201394	26.139076043715242	29.331996156583013
34	20.267720369675267	23.66223147167219	28.55708097407143	27.512967184581115
35	18.301664756069105	23.38752570611193	30.412196327304926	27.898613210514036
36	20.864739837480855	24.343597420910683	28.319186215871923	26.472476525736543
37	20.15011354891444	23.346222016750456	30.95704271381181	25.546621720523294
38	18.145435362624653	24.584897921917186	30.50357168219057	26.766095033267582
39	21.821898491473323	21.87037597951337	30.781973041009493	25.525752488003818
40	23.985704575724153	21.533497292072155	30.058578776280378	24.42221935592331
41	19.90562469293968	23.51419035348712	29.17967974423596	27.400505209337243
42	20.269097159320648	22.201457657921395	31.64406074685766	25.8853844359003
43	21.845521303283572	21.081113199644644	30.797697428012015	26.275668069059773
44	19.640991230574585	21.65907500025362	28.78910626062483	29.910827508546966
45	21.39777481808261	20.88256564025791	28.02868360069622	29.69097594096326
46	23.312092126067554	21.84972413483263	29.311489237128104	25.526694501971708
47	18.88390185084006	22.51478599616528	32.42057010685337	26.18074204614129
48	18.75912123140066	20.497789165679965	32.41470063520727	28.328388967712108
49	21.552989734946255	18.25079600180287	34.392495192105635	25.80371907114524
50	20.3917039003726	18.7320202141705	32.168327751296715	28.707948134160183
51	19.11991258110378	18.83832286731661	29.416197712790087	32.625566838789524
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	931.0
1	1287.5
2	1644.0
3	2384.5
4	3125.0
5	2458.0
6	1791.0
7	1842.0
8	1893.0
9	2137.5
10	2382.0
11	2530.0
12	2678.0
13	2804.5
14	2931.0
15	2964.5
16	2998.0
17	3234.5
18	3471.0
19	3441.5
20	3412.0
21	3390.5
22	3369.0
23	3760.0
24	4151.0
25	5518.0
26	8454.5
27	10024.0
28	10556.0
29	11088.0
30	13089.5
31	15091.0
32	18051.5
33	21012.0
34	22615.0
35	24218.0
36	27865.0
37	31512.0
38	33635.5
39	35759.0
40	41654.5
41	47550.0
42	57427.0
43	67304.0
44	82616.5
45	97929.0
46	117031.5
47	136134.0
48	162793.0
49	189452.0
50	185186.5
51	180921.0
52	151205.0
53	121489.0
54	100038.5
55	78588.0
56	71999.5
57	65411.0
58	58517.5
59	51624.0
60	45518.5
61	39413.0
62	36360.0
63	33307.0
64	30065.0
65	26823.0
66	22223.5
67	17624.0
68	14635.0
69	11646.0
70	10224.0
71	8802.0
72	7351.5
73	5901.0
74	5239.5
75	3595.5
76	2613.0
77	1982.0
78	1351.0
79	1005.0
80	659.0
81	440.5
82	222.0
83	156.5
84	91.0
85	143.5
86	196.0
87	108.5
88	21.0
89	12.0
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1380022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.472909140840223
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.656195936042295	13.66883711586159
2	12.572794172544894	5.399489338270839
3	6.275427189110971	4.042550335552148
4	3.7828366550006045	3.2491403114987176
5	2.424788565182203	2.603363227295289
6	1.7221527558948406	2.21877777923865
7	1.2412452321234442	1.8657202263621497
8	0.9847762576927771	1.6916808884394927
9	0.7510223983063914	1.4513972149412155
>10	4.649528582095516	20.10398023268945
>50	1.311443528792624	20.594685494212793
>100	0.6114382883065342	18.080333958296137
>500	0.010900292604610206	1.5973199871073416
>1k	0.004428243870622896	1.6447173381305837
>5k	0.0010219024316822068	1.7880065521036252
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	9473	0.686438332142531	TruSeq Adapter, Index 15 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	8913	0.6458592689102057	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGCT	6059	0.43905097165117657	TruSeq Adapter, Index 14 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3804	0.27564777952815245	TruSeq Adapter, Index 15 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTGC	3076	0.22289499732612958	TruSeq Adapter, Index 14 (95% over 22bp)
GCCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1934	0.14014269337735197	No Hit
CGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1681	0.12180965230989071	TruSeq Adapter, Index 14 (95% over 21bp)
AGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1676	0.12144733924531637	TruSeq Adapter, Index 14 (95% over 21bp)
ACCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1541	0.11166488650180939	TruSeq Adapter, Index 14 (95% over 21bp)
GGCTGTCTCTTATACACATCTGACGCACCTCGTTTCGTATGCCGTCTTCTG	1515	0.10978085856602286	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.246261291486658E-5	0.0	0.0	0.4642679609455501	0.0
2	7.246261291486658E-5	0.0	0.0	2.4092369541934837	0.0
3	7.246261291486658E-5	0.0	0.0	3.353134950022536	0.0
4	7.246261291486658E-5	0.0	0.0	4.462754941587888	0.0
5	7.246261291486658E-5	0.0	0.0	7.3725636257972695	0.0
6	7.246261291486658E-5	0.0	0.0	9.006305696575852	0.0
7	7.246261291486658E-5	0.0	0.0	10.632366730385458	0.0
8	7.246261291486658E-5	0.0	0.0	13.088414532521945	0.0
9	7.246261291486658E-5	0.0	0.0	14.050573106805544	0.0
10	7.246261291486658E-5	0.0	0.0	16.023150355574042	0.0
11	7.246261291486658E-5	0.0	0.0	19.038826917252045	0.0
12	7.246261291486658E-5	0.0	0.0	21.243719303025603	0.0
13	7.246261291486658E-5	0.0	0.0	22.11319819539109	0.0
14	7.246261291486658E-5	0.0	0.0	22.458627471156255	0.0
15	7.246261291486658E-5	0.0	0.0	23.104486740066463	0.0
16	7.246261291486658E-5	0.0	0.0	24.402147212145895	0.0
17	7.246261291486658E-5	0.0	0.0	25.904804416161483	0.0
18	7.246261291486658E-5	0.0	0.0	27.57716905962369	0.0
19	7.246261291486658E-5	0.0	0.0	28.548240535295815	0.0
20	7.246261291486658E-5	0.0	0.0	29.50213837170712	0.0
21	7.246261291486658E-5	0.0	0.0	30.64451146430999	0.0
22	1.4492522582973315E-4	0.0	0.0	31.747464895487173	0.0
23	1.4492522582973315E-4	0.0	0.0	32.84751982214776	0.0
24	1.4492522582973315E-4	0.0	0.0	33.78076581387833	0.0
25	1.4492522582973315E-4	0.0	0.0	34.55531868332534	0.0
26	1.4492522582973315E-4	0.0	0.0	35.2258152406266	0.0
27	1.4492522582973315E-4	0.0	0.0	35.88493516770023	0.0
28	1.4492522582973315E-4	0.0	0.0	36.562170748002565	0.0
29	1.4492522582973315E-4	0.0	0.0	37.275057933859024	0.0
30	1.4492522582973315E-4	0.0	0.0	38.04221961678872	0.0
31	1.4492522582973315E-4	0.0	0.0	38.71126692183168	0.0
32	1.4492522582973315E-4	0.0	0.0	39.3513291817087	0.0
33	1.4492522582973315E-4	0.0	0.0	39.97842063387395	0.0
34	1.4492522582973315E-4	0.0	0.0	40.612178646427374	0.0
35	1.4492522582973315E-4	0.0	0.0	41.31897897279899	0.0
36	1.4492522582973315E-4	0.0	0.0	41.96991062461323	0.0
37	1.4492522582973315E-4	0.0	0.0	42.565408377547605	0.0
38	1.4492522582973315E-4	0.0	0.0	43.18713759635716	0.0
39	1.4492522582973315E-4	0.0	0.0	43.80314226874644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	75	0.0	45.0	25
ACTATCG	20	7.0337794E-4	45.0	28
ACGCATT	20	7.0337794E-4	45.0	22
CTATCGA	20	7.0337794E-4	45.0	29
ATATGCG	40	6.8175723E-9	45.0	1
TACGGAT	35	1.2121745E-7	45.0	30
ATGTCGC	20	7.0337794E-4	45.0	35
AGGTTAC	20	7.0337794E-4	45.0	15
CGTAGGA	20	7.0337794E-4	45.0	19
CACTACG	20	7.0337794E-4	45.0	13
ACGGATG	45	3.8562575E-10	45.0	12
CGAATGT	20	7.0337794E-4	45.0	12
TTACGTC	20	7.0337794E-4	45.0	43
TTACGAG	45	3.8562575E-10	45.0	1
TACTACG	20	7.0337794E-4	45.0	1
AATAGCG	35	1.2121745E-7	45.0	1
CGCGTAG	20	7.0337794E-4	45.0	13
TATCGGA	20	7.0337794E-4	45.0	28
TTCGACA	20	7.0337794E-4	45.0	44
ATTCTCG	40	6.8175723E-9	45.0	12
>>END_MODULE