##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550079_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	586735
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.14140284796373	33.0	31.0	34.0	30.0	34.0
2	32.32991384526234	34.0	31.0	34.0	30.0	34.0
3	32.33346229558489	34.0	31.0	34.0	30.0	34.0
4	35.8844163037828	37.0	35.0	37.0	35.0	37.0
5	35.7986177746342	37.0	35.0	37.0	35.0	37.0
6	35.74159032612678	37.0	35.0	37.0	35.0	37.0
7	36.026940612030984	37.0	35.0	37.0	35.0	37.0
8	35.747190810161314	37.0	35.0	37.0	35.0	37.0
9	37.47818350703469	39.0	37.0	39.0	35.0	39.0
10	37.35592899690661	39.0	37.0	39.0	34.0	39.0
11	37.27036737198224	39.0	37.0	39.0	34.0	39.0
12	37.02619410807264	39.0	35.0	39.0	34.0	39.0
13	36.80327405046571	39.0	35.0	39.0	33.0	39.0
14	37.84258992560525	40.0	36.0	41.0	33.0	41.0
15	37.97308154447919	40.0	36.0	41.0	33.0	41.0
16	38.06621046980323	40.0	36.0	41.0	34.0	41.0
17	38.08637630276019	40.0	36.0	41.0	34.0	41.0
18	38.10492811916794	40.0	36.0	41.0	34.0	41.0
19	38.032938208901804	40.0	36.0	41.0	34.0	41.0
20	37.992330438784116	40.0	36.0	41.0	34.0	41.0
21	37.89599222817797	40.0	35.0	41.0	34.0	41.0
22	37.822504196954334	40.0	35.0	41.0	34.0	41.0
23	37.61820412963263	39.0	35.0	41.0	33.0	41.0
24	37.403891024056854	39.0	35.0	41.0	33.0	41.0
25	37.348140131405145	39.0	35.0	41.0	33.0	41.0
26	37.52965478452794	39.0	35.0	41.0	33.0	41.0
27	37.59390014231297	39.0	35.0	41.0	33.0	41.0
28	37.58805764101341	39.0	35.0	41.0	33.0	41.0
29	37.65007712169889	39.0	35.0	41.0	34.0	41.0
30	37.50303118102721	39.0	35.0	41.0	33.0	41.0
31	37.375871560414836	39.0	35.0	41.0	33.0	41.0
32	37.295971776014724	39.0	35.0	41.0	33.0	41.0
33	37.156196579375695	39.0	35.0	41.0	33.0	41.0
34	36.98021082771609	39.0	35.0	41.0	32.0	41.0
35	36.90956053414233	39.0	35.0	41.0	32.0	41.0
36	36.79159586525433	39.0	35.0	41.0	32.0	41.0
37	36.64855513988427	39.0	35.0	41.0	31.0	41.0
38	36.60507043213717	39.0	35.0	41.0	31.0	41.0
39	36.53939512727211	39.0	35.0	41.0	31.0	41.0
40	36.41487724441187	38.0	35.0	40.0	31.0	41.0
41	36.45958396891271	39.0	35.0	40.0	31.0	41.0
42	36.478517559034316	39.0	35.0	40.0	31.0	41.0
43	36.32478546532932	38.0	35.0	40.0	31.0	41.0
44	36.16310941055161	38.0	35.0	40.0	30.0	41.0
45	36.1992926960212	38.0	35.0	40.0	31.0	41.0
46	36.129329254263	38.0	35.0	40.0	31.0	41.0
47	36.08221258319343	38.0	35.0	40.0	30.0	41.0
48	36.05799722191449	38.0	35.0	40.0	30.0	41.0
49	36.02411139611579	38.0	35.0	40.0	30.0	41.0
50	35.91301183668948	38.0	35.0	40.0	30.0	41.0
51	34.97143003229738	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	7.0
10	13.0
11	11.0
12	7.0
13	10.0
14	16.0
15	17.0
16	53.0
17	76.0
18	185.0
19	310.0
20	534.0
21	925.0
22	1497.0
23	2125.0
24	2984.0
25	3613.0
26	4036.0
27	4591.0
28	5350.0
29	6461.0
30	8201.0
31	10643.0
32	14231.0
33	20359.0
34	41161.0
35	65583.0
36	43469.0
37	66199.0
38	123254.0
39	160688.0
40	121.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.65367670242955	22.539647370618763	25.757795256802474	12.048880670149217
2	31.872139892796582	24.79313489053832	24.268536903372052	19.066188313293054
3	31.362881028062073	25.150195573811008	26.412094045863977	17.074829352262945
4	27.69785337503302	27.723077709698586	26.259554995014785	18.31951392025361
5	23.854210163020785	30.34248851696251	25.232174661474087	20.571126658542614
6	25.16212600236904	33.1742609525595	25.959078630045934	15.704534415025522
7	76.41371317545399	7.46861871202502	10.072519962163499	6.045148150357487
8	74.96723393013882	5.984473399405183	10.20869728241881	8.83959538803719
9	69.17876042847281	8.309032186591903	13.440139074710048	9.07206831022523
10	38.63345462602367	27.569175181299904	19.451370721023974	14.345999471652448
11	32.33708573717266	23.6582102652816	25.151388616666807	18.853315380878932
12	28.875557108404987	20.921540388761535	30.505083214739194	19.697819288094284
13	22.59077777872464	30.098085166216435	29.90651657051309	17.404620484545834
14	17.014836340085388	32.964967148712795	29.050082234739705	20.97011427646212
15	14.50041330413219	26.7625077760829	39.17458477847751	19.562494141307404
16	16.62641567317443	26.15593070125355	34.018764859774855	23.198888765797165
17	16.955354631988886	25.670532693635117	27.90237500745652	29.471737666919477
18	19.576128916802304	25.485440616291854	33.02274450987243	21.915685957033414
19	21.428242733090748	27.989296701236505	27.224385795972626	23.35807476970012
20	24.00930573427527	27.080027610420377	27.348291818282526	21.562374837021824
21	20.05402779789854	29.87413397871271	28.97662488176093	21.095213341627822
22	19.110160464264105	26.54128354367815	26.403913180567034	27.944642811490706
23	17.3294587846302	29.01906312048881	27.057018926772734	26.59445916810826
24	21.353933206643543	25.268647685922947	29.77357750943782	23.603841597995686
25	16.81679122602197	27.885672407475266	27.63291775673856	27.6646186097642
26	16.336847128601498	29.537525458682367	28.61734854747032	25.50827886524581
27	19.94273394292142	28.615985069920836	28.701202416763955	22.74007857039379
28	15.538531023375118	26.309321925571172	33.3936104033337	24.758536647720007
29	18.782755417692826	24.0473126709673	30.806070883789104	26.363861027550765
30	20.416542391369187	27.681832513826514	29.229379532497635	22.672245562306664
31	21.77422516127383	28.88970318798095	26.22614979505228	23.109921855692946
32	25.73137787928111	27.947028897202316	23.84210929976906	22.479483923747516
33	24.284728199272244	28.594169429128996	25.687746597697426	21.433355773901337
34	21.04442380290932	26.854542510673475	28.500430347601558	23.60060333881565
35	22.685028164333133	25.208313804358013	26.352101033686417	25.754556997622437
36	25.01793825151048	27.183311034794244	27.60718211799194	20.19156859570334
37	21.661397394053534	27.40095613863158	31.616146982879833	19.32149948443505
38	23.873810152794704	28.845219732928832	25.418459781673157	21.862510332603303
39	25.465329322436876	26.970778971767494	26.464247062131967	21.099644643663666
40	24.53049502756781	26.408514917296564	27.77557159535395	21.285418459781674
41	21.95522680596862	25.10111038202937	25.58378143454882	27.359881377453192
42	26.15388548492931	21.342002778085504	27.864027201377112	24.640084535608068
43	26.331819305137756	22.22229797097497	28.90180405123267	22.544078672654607
44	21.51635746972654	24.600373251979175	28.281251331521045	25.60201794677325
45	22.938464553844582	25.200985112529505	25.245298132887932	26.61525220073798
46	25.066171269823684	25.59315534270156	27.801307234100577	21.539366153374182
47	21.215540235370312	24.221496927914647	31.327771481162703	23.235191355552338
48	22.456986544180936	21.131515931382992	30.631716192148073	25.779781332288
49	23.637587667345564	19.3692211986672	33.67755460301499	23.315636530972245
50	21.386145363750245	21.314051488320963	30.829250002130433	26.47055314579836
51	20.42864325462091	22.31075357699813	26.285801937842468	30.97480123053849
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1126.0
1	1216.5
2	1307.0
3	1495.5
4	1684.0
5	1380.0
6	1076.0
7	1130.5
8	1185.0
9	1389.5
10	1594.0
11	1586.5
12	1579.0
13	1662.0
14	1745.0
15	1758.0
16	1771.0
17	1784.5
18	1798.0
19	2074.5
20	2351.0
21	2029.0
22	1707.0
23	1852.5
24	1998.0
25	2048.0
26	3416.5
27	4735.0
28	4989.5
29	5244.0
30	5635.0
31	6026.0
32	6797.0
33	7568.0
34	9411.5
35	11255.0
36	11980.0
37	12705.0
38	14528.5
39	16352.0
40	19602.0
41	22852.0
42	29407.5
43	35963.0
44	42501.0
45	49039.0
46	57864.5
47	66690.0
48	74812.0
49	82934.0
50	81618.0
51	80302.0
52	64062.5
53	47823.0
54	38145.5
55	28468.0
56	23872.5
57	19277.0
58	17629.5
59	15982.0
60	15509.0
61	15036.0
62	12758.0
63	10480.0
64	9254.5
65	8029.0
66	6735.5
67	5442.0
68	4768.0
69	4094.0
70	3317.0
71	2540.0
72	2133.0
73	1726.0
74	1545.5
75	1170.5
76	976.0
77	677.5
78	379.0
79	274.0
80	169.0
81	151.5
82	134.0
83	123.5
84	113.0
85	61.5
86	10.0
87	5.5
88	1.0
89	2.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	586735.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.208562371557534
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.62814571785846	16.129716213120005
2	12.571277901199771	6.086651303227551
3	5.910809136386557	4.292765749337582
4	3.458720959330535	3.3492264827906624
5	2.1370032033079926	2.5866887667749894
6	1.4882984718244543	2.161773982959364
7	1.052257949437687	1.7831556539950637
8	0.824245711863961	1.5963042980118032
9	0.568664248850824	1.2389889543102082
>10	3.672747119547855	18.254490971345785
>50	1.1136414409053597	19.53588477905606
>100	0.5600106545609382	18.276966554010777
>500	0.009924239447915362	1.7214024966506618
>1k	0.004253245477678013	2.9859837944094823
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	4602	0.7843404603441076	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	4513	0.7691717726060316	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGCT	3573	0.6089631605409598	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1989	0.3389946057419448	Illumina Single End Adapter 1 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTGC	1487	0.2534363895114489	Illumina Single End Adapter 1 (95% over 21bp)
TGCAATGGGGTTTCTCTTGTTGAATTACAACGATGATTTTTCATGTCATTG	1236	0.210657281396201	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	945	0.1610607855335032	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	935	0.15935643859664073	No Hit
GCCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	840	0.14316514269644728	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	832	0.14180166514695733	No Hit
AAAACTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	800	0.13634775494899742	No Hit
ACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	763	0.1300416712826063	No Hit
AGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	756	0.12884862842680256	No Hit
GGCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTCTG	707	0.12049732843617646	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCCGTCTTC	657	0.11197559375186414	No Hit
AGACATAGACCTGTCTCTTATACACATCTGACGCCTTGAAGGTCGTATGCC	638	0.10873733457182544	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.7043469368624678E-4	0.0	0.0	0.6415161870350329	0.0
2	1.7043469368624678E-4	0.0	0.0	2.9040367457199587	0.0
3	1.7043469368624678E-4	0.0	0.0	3.956811848619905	0.0
4	1.7043469368624678E-4	0.0	0.0	5.230299879843541	0.0
5	1.7043469368624678E-4	0.0	0.0	8.429359080334393	0.0
6	1.7043469368624678E-4	0.0	0.0	10.067066051965538	0.0
7	1.7043469368624678E-4	0.0	0.0	11.747381697018245	0.0
8	1.7043469368624678E-4	0.0	0.0	14.401561181794166	0.0
9	1.7043469368624678E-4	0.0	0.0	15.324294613411507	0.0
10	1.7043469368624678E-4	0.0	0.0	17.36081876826847	0.0
11	1.7043469368624678E-4	0.0	0.0	20.362003289389587	0.0
12	1.7043469368624678E-4	0.0	0.0	22.730193358159987	0.0
13	1.7043469368624678E-4	0.0	0.0	23.63247462653498	0.0
14	1.7043469368624678E-4	0.0	0.0	23.962777062898923	0.0
15	1.7043469368624678E-4	0.0	0.0	24.602248033609722	0.0
16	1.7043469368624678E-4	0.0	0.0	25.898063009706256	0.0
17	1.7043469368624678E-4	0.0	0.0	27.48855957118631	0.0
18	1.7043469368624678E-4	0.0	0.0	29.351922077258045	0.0
19	1.7043469368624678E-4	0.0	0.0	30.345897210836238	0.0
20	1.7043469368624678E-4	0.0	0.0	31.226533273113073	0.0
21	1.7043469368624678E-4	0.0	0.0	32.31152053311972	0.0
22	1.7043469368624678E-4	0.0	0.0	33.253683519817294	0.0
23	1.7043469368624678E-4	0.0	0.0	34.27731429009689	0.0
24	1.7043469368624678E-4	0.0	0.0	34.9922878301107	0.0
25	1.7043469368624678E-4	0.0	0.0	35.65971009058604	0.0
26	1.7043469368624678E-4	0.0	0.0	36.30668018781903	0.0
27	1.7043469368624678E-4	0.0	0.0	36.86911467698365	0.0
28	1.7043469368624678E-4	0.0	0.0	37.46648827835394	0.0
29	1.7043469368624678E-4	0.0	0.0	38.10340272865945	0.0
30	1.7043469368624678E-4	0.0	0.0	38.81258148908792	0.0
31	1.7043469368624678E-4	0.0	0.0	39.47250462304107	0.0
32	1.7043469368624678E-4	0.0	0.0	40.02420172650345	0.0
33	1.7043469368624678E-4	0.0	0.0	40.5799892626143	0.0
34	1.7043469368624678E-4	0.0	0.0	41.106291596717426	0.0
35	1.7043469368624678E-4	0.0	0.0	41.71201649807835	0.0
36	1.7043469368624678E-4	0.0	0.0	42.251612738289005	0.0
37	1.7043469368624678E-4	0.0	0.0	42.791038543805975	0.0
38	1.7043469368624678E-4	0.0	0.0	43.2778000289739	0.0
39	1.7043469368624678E-4	0.0	0.0	43.75655108353857	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAC	25	3.888462E-5	45.000004	26
CTAGATG	30	2.163677E-6	45.000004	18
CGTGGTT	25	3.888462E-5	45.000004	44
GTTTGAA	30	2.163677E-6	45.000004	11
TTAGGCA	30	2.163677E-6	45.000004	13
AGGTACG	25	3.888462E-5	45.000004	1
GTACCTT	30	2.163677E-6	45.000004	20
GATACGT	30	2.163677E-6	45.000004	11
TCGTTGA	25	3.888462E-5	45.000004	24
ATCTCCG	25	3.888462E-5	45.000004	31
GGTACCG	25	3.888462E-5	45.000004	1
GATGTAC	25	3.888462E-5	45.000004	17
TCCGCAC	25	3.888462E-5	45.000004	34
TTAATCG	25	3.888462E-5	45.000004	20
ATAGGTG	30	2.163677E-6	45.000004	34
GTCGAGC	25	3.888462E-5	45.000004	13
TACTTAG	25	3.888462E-5	45.000004	9
CTCCGCA	25	3.888462E-5	45.000004	33
CTCCGAT	25	3.888462E-5	45.000004	35
CGAGTAC	30	2.163677E-6	45.000004	31
>>END_MODULE