SRR3550078_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445337
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	15341	3.444806966409707	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	13706	3.0776692706871422	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	12913	2.899601874535464	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	3822	0.8582264666982533	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	3699	0.8306069336255465	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	3558	0.798945517664151	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	3101	0.69632660210133	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	3042	0.6830782081884057	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	2986	0.6705034614235961	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2835	0.6365965549684846	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1828	0.41047566225128385	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	1715	0.3851016196722931	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1637	0.3675867938213083	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	1348	0.3026921185529161	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1216	0.27305164403586496	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1108	0.24880034670373224	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	1089	0.24453391476567185	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT	834	0.18727390717591397	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTAT	810	0.1818847299909956	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG	760	0.1706572775224156	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATG	737	0.16549264938686883	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	709	0.15920527600446405	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGCCTAA	666	0.14954966688148524	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	632	0.1419149992028509	No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	501	0.11249907373517135	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	484	0.10868173989585414	No Hit
GCTGACTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	483	0.10845719084648256	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	468	0.10508895510590857	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	453	0.10172071936533457	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	20	7.0286257E-4	45.000004	1
ATTAGCG	20	7.0286257E-4	45.000004	1
TTGTGCG	20	7.0286257E-4	45.000004	1
CATGCGA	20	7.0286257E-4	45.000004	18
ACCGCGA	20	7.0286257E-4	45.000004	30
TTATGCG	20	7.0286257E-4	45.000004	1
TTCCGTC	20	7.0286257E-4	45.000004	36
ATCACAT	20	7.0286257E-4	45.000004	32
CGTAAGC	20	7.0286257E-4	45.000004	43
TAGATAG	20	7.0286257E-4	45.000004	1
CAGTCGC	20	7.0286257E-4	45.000004	40
GCGTGAT	20	7.0286257E-4	45.000004	30
ATTATCC	20	7.0286257E-4	45.000004	45
AAGGATC	20	7.0286257E-4	45.000004	11
TAATACG	580	0.0	45.0	4
ACCTAGT	25	3.886943E-5	45.0	36
GTCCCGC	55	1.8189894E-12	45.0	15
CAATCGA	85	0.0	45.0	41
GCGTAAG	25	3.886943E-5	45.0	1
ATACCGC	30	2.1624946E-6	44.999996	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content