##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550078_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	445337
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.731803106411547	31.0	31.0	34.0	30.0	34.0
2	31.825808320440476	31.0	31.0	34.0	30.0	34.0
3	31.840033502718168	31.0	31.0	34.0	30.0	34.0
4	35.602083366080066	37.0	35.0	37.0	33.0	37.0
5	31.51251075028574	35.0	35.0	37.0	0.0	37.0
6	33.10120201106129	35.0	35.0	37.0	17.0	37.0
7	34.9817396713051	37.0	35.0	37.0	32.0	37.0
8	34.88370829282094	36.0	35.0	37.0	32.0	37.0
9	37.040138142575174	39.0	37.0	39.0	34.0	39.0
10	36.816830849446596	39.0	35.0	39.0	33.0	39.0
11	36.85246902907237	39.0	37.0	39.0	33.0	39.0
12	36.80600084879541	39.0	35.0	39.0	33.0	39.0
13	36.587299056669444	39.0	35.0	39.0	32.0	39.0
14	37.58429683587934	39.0	36.0	41.0	32.0	41.0
15	37.60521357982831	40.0	36.0	41.0	32.0	41.0
16	37.74341229226406	39.0	36.0	41.0	33.0	41.0
17	37.45837646546323	39.0	36.0	41.0	32.0	41.0
18	37.502675501923264	39.0	36.0	41.0	32.0	41.0
19	37.456658665235544	39.0	36.0	41.0	32.0	41.0
20	37.085501541529226	39.0	35.0	41.0	31.0	41.0
21	37.24593060985276	39.0	35.0	41.0	32.0	41.0
22	37.29827748424227	39.0	35.0	41.0	32.0	41.0
23	36.864935992293475	39.0	35.0	40.0	31.0	41.0
24	36.6328757772204	39.0	35.0	40.0	30.0	41.0
25	36.62410713684243	38.0	35.0	40.0	31.0	41.0
26	36.83773187496211	39.0	35.0	40.0	31.0	41.0
27	36.828377161565285	39.0	35.0	40.0	31.0	41.0
28	36.77944792370722	39.0	35.0	40.0	31.0	41.0
29	36.854301349315236	39.0	35.0	41.0	31.0	41.0
30	36.68556845714593	39.0	35.0	40.0	31.0	41.0
31	36.77353554723726	39.0	35.0	41.0	31.0	41.0
32	36.3706990436456	39.0	35.0	40.0	30.0	41.0
33	36.3592290782037	39.0	35.0	40.0	30.0	41.0
34	36.24994554685553	39.0	35.0	41.0	30.0	41.0
35	36.03617709734426	39.0	35.0	40.0	29.0	41.0
36	35.99087657212403	39.0	35.0	40.0	29.0	41.0
37	35.92417876798918	39.0	35.0	40.0	29.0	41.0
38	35.82059653700456	39.0	35.0	40.0	28.0	41.0
39	35.782766758656926	39.0	35.0	40.0	28.0	41.0
40	35.74311588751889	38.0	35.0	40.0	28.0	41.0
41	35.42864841681693	38.0	35.0	40.0	26.0	41.0
42	35.506138048264575	38.0	35.0	40.0	27.0	41.0
43	35.539699149183654	38.0	35.0	40.0	28.0	41.0
44	35.628708146864064	38.0	35.0	40.0	28.0	41.0
45	35.56450957364872	38.0	35.0	40.0	27.0	41.0
46	35.49688662743046	38.0	35.0	40.0	27.0	41.0
47	35.289401060320614	38.0	35.0	40.0	27.0	41.0
48	35.29200133831233	38.0	35.0	40.0	27.0	41.0
49	35.30165245645433	38.0	35.0	40.0	27.0	41.0
50	35.238379025322395	38.0	35.0	40.0	27.0	41.0
51	34.32693892490406	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	8.0
10	15.0
11	15.0
12	21.0
13	24.0
14	17.0
15	31.0
16	55.0
17	84.0
18	155.0
19	359.0
20	930.0
21	1786.0
22	2869.0
23	3576.0
24	4178.0
25	4565.0
26	5057.0
27	5288.0
28	5506.0
29	6520.0
30	8168.0
31	10943.0
32	14922.0
33	20936.0
34	31516.0
35	39765.0
36	44052.0
37	61984.0
38	86240.0
39	85715.0
40	35.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	54.34603457606262	15.920303051397031	17.800676790834807	11.93298558170554
2	27.393412179989536	39.20985680507121	18.910398192829252	14.486332822109999
3	24.826816545672152	39.14159389406225	23.13888134154584	12.892708218719756
4	23.206246056357323	19.44774406797549	42.22644873432928	15.11956114133791
5	31.89315058034702	31.497719704403632	19.267206632280722	17.341923082968627
6	20.953569993061436	42.497030338822064	24.046957697204586	12.502441970911915
7	63.06931604605052	5.024734077788281	26.53473661519254	5.371213260968659
8	60.1030231038517	19.15425846044681	13.628330904461116	7.114387531240387
9	56.24684227899321	6.011178051677718	13.764632177429679	23.977347491899394
10	41.12346380381599	19.21353940948091	21.899819687113354	17.76317709958975
11	33.64238767495178	19.232176980578753	25.557948250426083	21.567487094043386
12	22.79217760931609	16.509070658849364	31.7770587218219	28.921693010012646
13	25.12299674179329	18.68315455486519	38.38957912771676	17.80426957562475
14	19.999011984182765	31.470549269429664	26.48398853003456	22.04645021635301
15	14.861105185511198	21.93305294642035	39.939416666479545	23.26642520158891
16	16.33684153798135	22.746594152293657	28.220201779775767	32.696362529949226
17	16.474041007147395	26.12852738487932	32.39097582280385	25.006455785169436
18	16.72755688388793	23.769639621230667	30.28582848494511	29.2169750099363
19	17.722533721653488	26.470066488973522	26.684735380172768	29.122664409200222
20	22.329606567610597	24.590590945733233	33.2792918621179	19.80051062453827
21	20.557914568068675	32.465750656244595	27.729786655948192	19.246548119738534
22	17.29589052784745	20.31562614379672	32.448909477541726	29.939573850814106
23	20.743167533800246	29.473634573368034	28.16878004746967	21.614417845362052
24	22.585143385795476	24.987144566923476	25.350015830707978	27.077696216573067
25	19.41114257292792	33.66214799129648	22.961936690641018	23.96477274513458
26	17.375829989423742	21.370782126793866	30.989116107576958	30.264271776205437
27	24.704661862814003	24.409828960989095	25.965280225986163	24.920228950210742
28	17.31856998183398	25.214163655838163	32.027206362821865	25.440059999505994
29	22.07451884752446	19.663311155372224	26.94319133599948	31.318978661103834
30	19.695646218481734	26.678672555839732	31.59719493327525	22.028486292403283
31	26.758612017416024	20.060538423710582	22.674064809346632	30.506784749526762
32	24.119037942052874	26.879194856928574	21.814041950253404	27.187725250765148
33	21.411650053779496	20.583513159697038	22.97743057504766	35.02740621147581
34	22.441656543247024	20.933809676716734	26.34252262893045	30.28201115110579
35	18.567287245389448	24.337973265190183	27.518036902390776	29.576702587029597
36	22.545398204056703	27.231736864441984	22.9684486130728	27.254416318428515
37	17.775078199206444	22.813734318055765	33.60915441564478	25.80203306709301
38	23.27787720310686	24.42756833588945	21.44825154882707	30.846302912176622
39	21.424000251494935	21.513370773144832	28.064813837610618	28.997815137749615
40	17.952921046308752	23.04457074080977	29.9759507968123	29.026557416069178
41	18.44692895492627	27.321331935141252	28.98344399858983	25.248295111342646
42	19.28225141858862	26.651726669915142	30.339271158695553	23.726750752800687
43	21.978187305344044	24.745754338849007	24.352344404349964	28.923713951456985
44	21.816736538845863	18.958900787493516	31.74876554160108	27.475597132059544
45	21.345183535165503	18.18869754814893	28.201339659628548	32.264779257057015
46	28.120052903756033	23.590898577930872	24.579588042314025	23.709460475999077
47	17.02755441384839	21.459029903196907	37.234498817749255	24.278916865205453
48	20.98118952613414	24.870603610299614	26.80037814059914	27.347828722967098
49	19.584718988092163	20.10949011647359	35.447312933800696	24.85847796163355
50	21.577591801265108	19.097896649054537	30.922649588962965	28.40186196071739
51	19.732023164479934	19.250814551676594	27.998122769947255	33.01903951389622
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1620.0
1	1733.5
2	1847.0
3	3503.0
4	5159.0
5	3293.5
6	1428.0
7	1313.5
8	1199.0
9	1158.0
10	1117.0
11	1152.5
12	1188.0
13	1151.0
14	1114.0
15	1049.5
16	985.0
17	1142.5
18	1300.0
19	1077.5
20	855.0
21	940.0
22	1025.0
23	1187.0
24	1349.0
25	1404.5
26	1985.5
27	2511.0
28	2647.0
29	2783.0
30	3329.0
31	3875.0
32	4186.5
33	4498.0
34	5048.5
35	5599.0
36	6408.5
37	7218.0
38	7637.0
39	8056.0
40	9556.0
41	11056.0
42	13585.5
43	16115.0
44	22783.5
45	29452.0
46	59558.5
47	89665.0
48	67902.0
49	46139.0
50	44654.0
51	43169.0
52	35608.5
53	28048.0
54	25261.0
55	22474.0
56	21462.0
57	20450.0
58	19158.0
59	17866.0
60	16577.0
61	15288.0
62	14220.0
63	13152.0
64	11454.0
65	9756.0
66	8912.5
67	8069.0
68	6738.5
69	5408.0
70	4772.5
71	4137.0
72	3720.0
73	3303.0
74	2793.5
75	1893.5
76	1503.0
77	1204.5
78	906.0
79	711.0
80	516.0
81	358.0
82	200.0
83	156.5
84	113.0
85	77.0
86	41.0
87	37.0
88	33.0
89	20.0
90	7.0
91	3.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	445337.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.15960244096108
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.09406544417864	23.82835687919337
2	9.901591028900047	6.368624620448246
3	3.738846351129949	3.607194367205314
4	1.9884386298271814	2.557895832539661
5	1.2879677775474543	2.071026584134717
6	1.0079504737987701	1.944917191052808
7	0.8025151628004044	1.8065998011952917
8	0.7005806587246002	1.802431636992782
9	0.6680211849458045	1.9334966156997127
>10	5.655184613077532	30.020306835742634
>50	0.07601171321600798	1.6948578246656605
>100	0.061231657868450876	4.122614906311477
>500	0.0056304972752598504	1.2786126938483329
>1k	0.009853370231704739	7.4657038580091015
>5k	0.0	0.0
>10k+	0.0021114364782224442	9.497360352960886
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	15341	3.444806966409707	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	13706	3.0776692706871422	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	12913	2.899601874535464	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	3822	0.8582264666982533	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	3699	0.8306069336255465	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCT	3558	0.798945517664151	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTA	3101	0.69632660210133	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTC	3042	0.6830782081884057	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCT	2986	0.6705034614235961	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2835	0.6365965549684846	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGC	1828	0.41047566225128385	No Hit
CTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGCT	1715	0.3851016196722931	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCG	1637	0.3675867938213083	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTC	1348	0.3026921185529161	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1216	0.27305164403586496	No Hit
CCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	1108	0.24880034670373224	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTT	1089	0.24453391476567185	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCCTAATCGT	834	0.18727390717591397	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTAT	810	0.1818847299909956	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTG	760	0.1706572775224156	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATG	737	0.16549264938686883	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	709	0.15920527600446405	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTGCCTAA	666	0.14954966688148524	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCCTAATCGTATGCC	632	0.1419149992028509	No Hit
GAATAATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCCTAAT	501	0.11249907373517135	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	484	0.10868173989585414	No Hit
GCTGACTCTTATACACATCTGACGCCTGCCTAATCGTATGCCGTCTTCTGC	483	0.10845719084648256	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	468	0.10508895510590857	No Hit
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	453	0.10172071936533457	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4028409945726495	0.0
2	0.0	0.0	0.0	1.7600154489745967	0.0
3	2.245490493715995E-4	0.0	0.0	2.342944781143269	0.0
4	2.245490493715995E-4	0.0	0.0	3.6058086348091445	0.0
5	2.245490493715995E-4	0.0	0.0	7.927030540916205	0.0
6	2.245490493715995E-4	0.0	0.0	9.25725910939356	0.0
7	2.245490493715995E-4	0.0	0.0	11.066226251131166	0.0
8	2.245490493715995E-4	0.0	0.0	13.09390416695671	0.0
9	2.245490493715995E-4	0.0	0.0	13.582073800290566	0.0
10	2.245490493715995E-4	0.0	0.0	18.115270009004416	0.0
11	2.245490493715995E-4	0.0	0.0	19.47558815009757	0.0
12	2.245490493715995E-4	0.0	0.0	24.669856760161405	0.0
13	2.245490493715995E-4	0.0	0.0	25.319477159993443	0.0
14	2.245490493715995E-4	0.0	0.0	25.724114546961065	0.0
15	2.245490493715995E-4	0.0	0.0	26.89670968277956	0.0
16	2.245490493715995E-4	0.0	0.0	27.72237653731893	0.0
17	2.245490493715995E-4	0.0	0.0	28.536591390340348	0.0
18	2.245490493715995E-4	0.0	0.0	29.35237808670737	0.0
19	2.245490493715995E-4	0.0	0.0	30.954984652072476	0.0
20	2.245490493715995E-4	0.0	0.0	31.598317678522108	0.0
21	2.245490493715995E-4	0.0	0.0	32.15969030195111	0.0
22	2.245490493715995E-4	0.0	0.0	32.87667541659462	0.0
23	2.245490493715995E-4	0.0	0.0	33.46162569020764	0.0
24	2.245490493715995E-4	0.0	0.0	33.95810363836825	0.0
25	2.245490493715995E-4	0.0	0.0	34.345675297583625	0.0
26	2.245490493715995E-4	0.0	0.0	34.7539054693412	0.0
27	2.245490493715995E-4	0.0	0.0	35.234215885947044	0.0
28	2.245490493715995E-4	0.0	0.0	35.657041745913766	0.0
29	2.245490493715995E-4	0.0	0.0	36.06347552527637	0.0
30	2.245490493715995E-4	0.0	0.0	36.46452012745404	0.0
31	2.245490493715995E-4	0.0	0.0	36.866462925829204	0.0
32	2.245490493715995E-4	0.0	0.0	37.25964831127887	0.0
33	2.245490493715995E-4	0.0	0.0	37.68157597504811	0.0
34	2.245490493715995E-4	0.0	0.0	38.08464151867013	0.0
35	2.245490493715995E-4	0.0	0.0	38.49309623947707	0.0
36	2.245490493715995E-4	0.0	0.0	38.84833283558294	0.0
37	2.245490493715995E-4	0.0	0.0	39.21097955031807	0.0
38	2.245490493715995E-4	0.0	0.0	39.581934579879956	0.0
39	6.736471481147984E-4	0.0	0.0	39.94480584366446	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTATG	20	7.0286257E-4	45.000004	1
ATTAGCG	20	7.0286257E-4	45.000004	1
TTGTGCG	20	7.0286257E-4	45.000004	1
CATGCGA	20	7.0286257E-4	45.000004	18
ACCGCGA	20	7.0286257E-4	45.000004	30
TTATGCG	20	7.0286257E-4	45.000004	1
TTCCGTC	20	7.0286257E-4	45.000004	36
ATCACAT	20	7.0286257E-4	45.000004	32
CGTAAGC	20	7.0286257E-4	45.000004	43
TAGATAG	20	7.0286257E-4	45.000004	1
CAGTCGC	20	7.0286257E-4	45.000004	40
GCGTGAT	20	7.0286257E-4	45.000004	30
ATTATCC	20	7.0286257E-4	45.000004	45
AAGGATC	20	7.0286257E-4	45.000004	11
TAATACG	580	0.0	45.0	4
ACCTAGT	25	3.886943E-5	45.0	36
GTCCCGC	55	1.8189894E-12	45.0	15
CAATCGA	85	0.0	45.0	41
GCGTAAG	25	3.886943E-5	45.0	1
ATACCGC	30	2.1624946E-6	44.999996	28
>>END_MODULE