FastQCFastQC Report
Sat 18 Jun 2016
SRR3550076_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550076_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences796861
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC29910.3753477708157382No Hit
GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC27190.34121383779605224No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG27010.33895497458151425No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC26460.3320528925370924No Hit
CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT25610.32138603846844055No Hit
CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC21030.2639105188985281No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.21898423940938258No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA11000.13804164088843598No Hit
TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC9720.12197861358505435No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCC8600.10792346469459542No Hit
ACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC7970.10001744344371227No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG302.1646592E-645.0000041
TGTCACG302.1646592E-645.0000041
ATTCGGC302.1646592E-645.00000417
CATATAG207.0319837E-445.014
ATTAGCG253.8897248E-545.01
CGTAAAC207.0319837E-445.040
ATAACGG502.1827873E-1145.02
GCGTAAG551.8189894E-1245.01
AGTCGTA207.0319837E-445.043
CGAAAGG1200.041.2500042
TAACGCC556.002665E-1140.90909212
CACGACG451.9270374E-840.026
CGTTATT1800.040.01
TACGTAG451.9270374E-840.01
TAGTAAG900.040.01
CGACGGT403.4564437E-739.37528
GTTACGG403.4564437E-739.3752
GCCGATT403.4564437E-739.3759
CGGTCTA403.4564437E-739.37531
TGCGTAG700.038.571431