##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3550076_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 796861 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.970067552559353 33.0 31.0 34.0 30.0 34.0 2 32.04851661707625 33.0 31.0 34.0 30.0 34.0 3 32.0256983338374 33.0 31.0 34.0 30.0 34.0 4 35.739826644797525 37.0 35.0 37.0 35.0 37.0 5 31.6342134952018 37.0 35.0 37.0 0.0 37.0 6 33.30316328694716 37.0 35.0 37.0 17.0 37.0 7 35.24093537015866 37.0 35.0 37.0 32.0 37.0 8 35.399615491283924 37.0 35.0 37.0 33.0 37.0 9 37.41777800645282 39.0 37.0 39.0 35.0 39.0 10 37.04973012859206 39.0 37.0 39.0 33.0 39.0 11 37.0802825084927 39.0 37.0 39.0 33.0 39.0 12 37.07048782660966 39.0 37.0 39.0 33.0 39.0 13 36.96482197020559 39.0 37.0 39.0 33.0 39.0 14 37.99552117621518 40.0 37.0 41.0 33.0 41.0 15 38.16165554594841 40.0 37.0 41.0 33.0 41.0 16 38.07961614384441 40.0 37.0 41.0 33.0 41.0 17 38.08465717358485 40.0 37.0 41.0 33.0 41.0 18 38.07074006633528 40.0 37.0 41.0 33.0 41.0 19 38.044443133746036 40.0 37.0 41.0 34.0 41.0 20 38.08415771382964 40.0 37.0 41.0 34.0 41.0 21 37.976342172599736 40.0 37.0 41.0 33.0 41.0 22 37.96638058582363 40.0 37.0 41.0 33.0 41.0 23 37.743109526002655 40.0 36.0 41.0 33.0 41.0 24 37.49872185989777 39.0 36.0 41.0 32.0 41.0 25 37.345269752190156 39.0 35.0 41.0 32.0 41.0 26 37.62208339973973 39.0 36.0 41.0 33.0 41.0 27 37.72649935183175 39.0 36.0 41.0 33.0 41.0 28 37.663916291548965 40.0 36.0 41.0 33.0 41.0 29 37.66232253805871 40.0 36.0 41.0 33.0 41.0 30 37.46968919297092 39.0 36.0 41.0 33.0 41.0 31 37.48001596263338 39.0 36.0 41.0 33.0 41.0 32 37.365935840755164 39.0 35.0 41.0 33.0 41.0 33 37.311499747132814 39.0 35.0 41.0 33.0 41.0 34 37.276213793873715 39.0 35.0 41.0 33.0 41.0 35 37.18847954662105 39.0 35.0 41.0 32.0 41.0 36 37.081241270434866 39.0 35.0 41.0 32.0 41.0 37 37.06645826561972 39.0 35.0 41.0 32.0 41.0 38 36.90606642814744 39.0 35.0 41.0 31.0 41.0 39 36.86794434663009 39.0 35.0 41.0 31.0 41.0 40 36.82912452736424 39.0 35.0 41.0 31.0 41.0 41 36.751955485335586 39.0 35.0 41.0 31.0 41.0 42 36.78647217017774 39.0 35.0 41.0 31.0 41.0 43 36.691778114376284 39.0 35.0 41.0 31.0 41.0 44 36.62818860503902 39.0 35.0 41.0 31.0 41.0 45 36.53433283847497 39.0 35.0 40.0 31.0 41.0 46 36.445659908064265 39.0 35.0 40.0 31.0 41.0 47 36.39647692633972 39.0 35.0 40.0 31.0 41.0 48 36.32441918979596 38.0 35.0 40.0 31.0 41.0 49 36.292785065400366 38.0 35.0 40.0 31.0 41.0 50 36.18807922586248 38.0 35.0 40.0 31.0 41.0 51 35.15447612569821 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 21.0 10 19.0 11 18.0 12 17.0 13 22.0 14 36.0 15 44.0 16 74.0 17 131.0 18 233.0 19 417.0 20 732.0 21 1200.0 22 1671.0 23 2463.0 24 3158.0 25 4152.0 26 5356.0 27 6337.0 28 7647.0 29 9333.0 30 11901.0 31 16000.0 32 21551.0 33 31806.0 34 52058.0 35 66135.0 36 75907.0 37 116197.0 38 171204.0 39 190947.0 40 71.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.994275036675155 20.774263014503155 25.345825683525735 11.885636265295956 2 31.880089501180258 26.16441261399416 24.687869026091125 17.26762885873446 3 31.40208894650384 25.4155994583748 26.779576362753353 16.402735232368006 4 29.138206035933496 25.523020953466162 26.774180189518624 18.564592821081717 5 23.40897596946017 37.265470389440566 21.541649045442053 17.78390459565721 6 26.000268553737726 35.354848587143806 23.855728916335472 14.789153942782995 7 78.42697785435602 6.025015655177001 10.464309333748295 5.083697156718675 8 77.7604877136665 6.469258754036149 9.604686388215763 6.16556714408159 9 70.79202018921744 7.777391540055293 12.305031868795185 9.12555640193208 10 38.7273313664491 25.41647790518045 19.006326071924715 16.849864656445728 11 28.94181042866949 23.763115524539412 25.6889219073339 21.606152139457198 12 25.602457643177416 20.511105450009477 31.754346115570968 22.13209079124214 13 24.5810749930038 21.864039023116955 33.723321884243305 19.831564099635944 14 21.02499683131688 27.255820023818455 29.902329264451392 21.816853880413273 15 19.369626572262916 23.92537719878373 35.23324645076117 21.47174977819218 16 21.06214258195595 24.239484678005326 30.411953904131334 24.28641883590739 17 21.342241620558667 24.218527447070443 28.790968562898673 25.64826236947222 18 21.543154954251744 23.928514508803918 30.903758622896593 23.624571914047745 19 22.857938837513693 25.56418246093108 28.430177910576624 23.147700790978604 20 23.655694029448046 25.34268837350554 28.99087795738529 22.01073963966112 21 23.117457122383954 26.49596353692802 29.11360952537519 21.272969815312834 22 21.03315383736938 23.804904494008365 27.99961348340551 27.162328185216744 23 20.907786928962516 25.867999563286446 28.2170918140052 25.007121693745837 24 21.83969349736027 24.55246272561965 28.602478976885553 25.005364800134526 25 22.109000189493525 26.256022066583757 26.584310187096623 25.050667556826095 26 20.992619791908503 25.272663613854863 28.40181662799409 25.332899966242543 27 23.809171235635826 24.172597228374837 28.167271330884557 23.85096020510478 28 21.661117811010953 24.642189792197133 29.16255156169018 24.53414083510173 29 23.585292792594945 24.057897174036626 27.586116022744246 24.770694010624187 30 24.36736143442834 25.51724830302901 27.9716286780254 22.14376158451725 31 26.3859067014197 25.937020383730662 24.01937100698867 23.65770190786097 32 24.69288872212343 27.604437913262164 24.859668122796823 22.843005241817583 33 23.501212884053807 26.140820042642314 26.3107367533359 24.047230319967973 34 25.30792697848182 24.512807126964425 26.035531918364686 24.143733976189072 35 23.69647905971054 24.27148524021128 27.186422726171816 24.845612973906363 36 25.33164504223447 25.73535409563274 27.21967821238585 21.713322649746942 37 24.3405060606555 26.113713684067864 28.205672005531707 21.340108249744937 38 22.55387075035671 27.73331860889164 25.616638284468685 24.096172356282967 39 24.363847647205723 25.60346158238388 27.415948327249044 22.616742443161357 40 23.467580920637353 24.140847650970496 28.85396574810412 23.537605680288028 41 22.295230912292105 23.682925880423312 27.661034986026422 26.360808221258164 42 23.043667590709045 23.255624255673197 28.972932544069796 24.727775609547965 43 23.117331629983145 22.61285217873632 30.182804780256532 24.087011411024008 44 23.30117799716638 23.616289415594437 28.32024656746911 24.762286019770073 45 23.61779532440413 22.900480761387495 27.71035349954383 25.771370414664542 46 25.199376051783183 24.15502829226176 27.4233523788967 23.222243277058357 47 22.176766085929668 23.441478501269355 30.406432238495796 23.97532317430518 48 21.669274817063453 23.168030559909443 29.531624712465536 25.631069910561564 49 23.135528028100257 22.159197149816592 31.398449666880424 23.306825155202727 50 22.057171827959955 21.73729169830121 30.834110340448333 25.3714261332905 51 21.675298452302222 21.82927762809323 28.12196857419299 28.373455345411557 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 452.0 1 892.5 2 1333.0 3 2453.0 4 3573.0 5 2490.0 6 1407.0 7 1376.0 8 1345.0 9 1296.0 10 1247.0 11 1401.0 12 1555.0 13 1558.5 14 1562.0 15 1607.0 16 1652.0 17 1741.0 18 1830.0 19 1909.5 20 1989.0 21 2069.5 22 2150.0 23 2717.0 24 3284.0 25 4357.0 26 5644.5 27 5859.0 28 7326.5 29 8794.0 30 9982.0 31 11170.0 32 12354.5 33 13539.0 34 14602.0 35 15665.0 36 17313.5 37 18962.0 38 20781.5 39 22601.0 40 25992.5 41 29384.0 42 33426.0 43 37468.0 44 45052.5 45 52637.0 46 67338.0 47 82039.0 48 86008.0 49 89977.0 50 85369.5 51 80762.0 52 69624.5 53 58487.0 54 52021.0 55 45555.0 56 42657.0 57 39759.0 58 36537.5 59 33316.0 60 30996.5 61 28677.0 62 26911.0 63 25145.0 64 22874.5 65 20604.0 66 17722.5 67 14841.0 68 12952.5 69 11064.0 70 9730.5 71 8397.0 72 6885.5 73 5374.0 74 4504.5 75 2780.5 76 1926.0 77 1605.5 78 1285.0 79 942.0 80 599.0 81 480.5 82 362.0 83 227.0 84 92.0 85 67.5 86 43.0 87 30.0 88 17.0 89 15.5 90 14.0 91 8.0 92 2.0 93 1.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 796861.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.678049562002066 #Duplication Level Percentage of deduplicated Percentage of total 1 71.685157605286 23.425311330854655 2 10.985332836556893 7.179585017761898 3 4.263331305841239 4.179520551345449 4 2.3046199746619194 3.0124194301432863 5 1.4103208581756355 2.304326745089435 6 0.9662090073272438 1.8944295497215495 7 0.7247530242330736 1.6578460672269486 8 0.5730449828102472 1.4980793879623902 9 0.5189943941563354 1.5263752081177768 >10 6.387099042185504 43.94266325472375 >50 0.11854104083704378 2.512912558262368 >100 0.05359776328907248 3.230503989602233 >500 0.005868368243329101 1.2624649529986167 >1k 0.003129796396442187 2.3735619561896018 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGC 2991 0.3753477708157382 No Hit GCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 2719 0.34121383779605224 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCG 2701 0.33895497458151425 No Hit GAATCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTC 2646 0.3320528925370924 No Hit CTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGCT 2561 0.32138603846844055 No Hit CCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 2103 0.2639105188985281 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1745 0.21898423940938258 No Hit GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA 1100 0.13804164088843598 No Hit TCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 972 0.12197861358505435 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCC 860 0.10792346469459542 No Hit ACTGTCTCTTATACACATCTGACGCGTGAAGCTTCGTATGCCGTCTTCTGC 797 0.10001744344371227 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.509848016153382E-4 0.0 0.0 0.3404608833912062 0.0 2 2.509848016153382E-4 0.0 0.0 1.218531211842467 0.0 3 2.509848016153382E-4 0.0 0.0 1.7822430762705164 0.0 4 2.509848016153382E-4 0.0 0.0 2.533315095104416 0.0 5 2.509848016153382E-4 0.0 0.0 4.388594748644996 0.0 6 2.509848016153382E-4 0.0 0.0 5.526434346768132 0.0 7 2.509848016153382E-4 0.0 0.0 6.681968373405148 0.0 8 2.509848016153382E-4 0.0 0.0 8.61580626985133 0.0 9 2.509848016153382E-4 0.0 0.0 9.360980145847268 0.0 10 2.509848016153382E-4 0.0 0.0 11.077214219292951 0.0 11 2.509848016153382E-4 0.0 0.0 12.8861871769355 0.0 12 2.509848016153382E-4 0.0 0.0 14.726533234779968 0.0 13 2.509848016153382E-4 0.0 0.0 15.333038007883433 0.0 14 2.509848016153382E-4 0.0 0.0 15.592430800352885 0.0 15 3.764772024230073E-4 0.0 0.0 16.09766320600456 0.0 16 3.764772024230073E-4 0.0 0.0 16.992173038961624 0.0 17 3.764772024230073E-4 0.0 0.0 18.056599582612275 0.0 18 3.764772024230073E-4 0.0 0.0 19.23547519579952 0.0 19 3.764772024230073E-4 0.0 0.0 19.99821800790853 0.0 20 3.764772024230073E-4 0.0 0.0 20.70097545243148 0.0 21 3.764772024230073E-4 0.0 0.0 21.462463340532413 0.0 22 3.764772024230073E-4 0.0 0.0 22.26975595492815 0.0 23 3.764772024230073E-4 0.0 0.0 23.053205013170427 0.0 24 3.764772024230073E-4 0.0 0.0 23.691333871277426 0.0 25 3.764772024230073E-4 0.0 0.0 24.24990054727236 0.0 26 3.764772024230073E-4 0.0 0.0 24.767054731000766 0.0 27 3.764772024230073E-4 0.0 0.0 25.303785729255164 0.0 28 3.764772024230073E-4 0.0 0.0 25.836249985882105 0.0 29 5.019696032306764E-4 0.0 0.0 26.401342266719038 0.0 30 5.019696032306764E-4 0.0 0.0 27.036835784409075 0.0 31 5.019696032306764E-4 0.0 0.0 27.59389655159432 0.0 32 5.019696032306764E-4 0.0 0.0 28.133890352269717 0.0 33 5.019696032306764E-4 0.0 0.0 28.65480930802235 0.0 34 5.019696032306764E-4 0.0 0.0 29.179618528200024 0.0 35 5.019696032306764E-4 0.0 0.0 29.719486836474616 0.0 36 5.019696032306764E-4 0.0 0.0 30.235762573397366 0.0 37 5.019696032306764E-4 0.0 0.0 30.732336003393314 0.0 38 5.019696032306764E-4 0.0 0.0 31.229160418190876 0.0 39 5.019696032306764E-4 0.0 0.0 31.759993273607318 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAACG 30 2.1646592E-6 45.000004 1 TGTCACG 30 2.1646592E-6 45.000004 1 ATTCGGC 30 2.1646592E-6 45.000004 17 CATATAG 20 7.0319837E-4 45.0 14 ATTAGCG 25 3.8897248E-5 45.0 1 CGTAAAC 20 7.0319837E-4 45.0 40 ATAACGG 50 2.1827873E-11 45.0 2 GCGTAAG 55 1.8189894E-12 45.0 1 AGTCGTA 20 7.0319837E-4 45.0 43 CGAAAGG 120 0.0 41.250004 2 TAACGCC 55 6.002665E-11 40.909092 12 CACGACG 45 1.9270374E-8 40.0 26 CGTTATT 180 0.0 40.0 1 TACGTAG 45 1.9270374E-8 40.0 1 TAGTAAG 90 0.0 40.0 1 CGACGGT 40 3.4564437E-7 39.375 28 GTTACGG 40 3.4564437E-7 39.375 2 GCCGATT 40 3.4564437E-7 39.375 9 CGGTCTA 40 3.4564437E-7 39.375 31 TGCGTAG 70 0.0 38.57143 1 >>END_MODULE