SRR3550074_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537401
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	6006	1.1176011953829637	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	5855	1.0895029968310443	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	5190	0.965759274731532	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2463	0.4583169737309756	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1537	0.2860061667172186	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	1496	0.2783768545276246	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	1485	0.2763299658913921	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGT	1410	0.26237390700798846	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	1311	0.24395190928189564	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	1293	0.24060245514987877	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTC	1147	0.21343466052351967	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTA	1101	0.20487494440836543	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	999	0.1858947043269365	No Hit
GAATATGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	690	0.1283957417273135	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	688	0.12802358015708942	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	645	0.12002210639727132	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	586	0.10904334007566045	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	560	0.10420523966274718	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	549	0.10215835102651465	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTA	35	1.2103192E-7	45.000004	41
CGGTCTA	45	3.8380676E-10	45.000004	31
TGAGACG	30	2.1633368E-6	45.000004	1
GGTGTCG	25	3.888024E-5	45.0	9
CGTATAC	20	7.0299313E-4	45.0	39
GGCCGAT	50	2.1827873E-11	45.0	8
CACGCTA	20	7.0299313E-4	45.0	25
GCACGAT	20	7.0299313E-4	45.0	17
TAGCACG	20	7.0299313E-4	45.0	2
GCCGATT	20	7.0299313E-4	45.0	9
TCGCTAG	20	7.0299313E-4	45.0	1
GCGATAT	25	3.888024E-5	45.0	9
TCGTAAG	20	7.0299313E-4	45.0	1
CGTAAGG	75	0.0	45.0	2
ATTATGA	20	7.0299313E-4	45.0	40
CGTCCAG	20	7.0299313E-4	45.0	14
TGACTAG	25	3.888024E-5	45.0	1
CGAGACA	80	0.0	42.1875	22
TCAAGCG	80	0.0	42.1875	17
GGTACCC	60	3.6379788E-12	41.250004	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content