##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550074_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	537401
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97221627797492	33.0	31.0	34.0	30.0	34.0
2	32.073846159571715	33.0	31.0	34.0	30.0	34.0
3	32.06162809522126	33.0	31.0	34.0	30.0	34.0
4	35.742894040018534	37.0	35.0	37.0	35.0	37.0
5	31.40367062956712	37.0	35.0	37.0	0.0	37.0
6	33.189411631165555	37.0	35.0	37.0	17.0	37.0
7	35.1434794501685	37.0	35.0	37.0	32.0	37.0
8	35.287414798260514	37.0	35.0	37.0	33.0	37.0
9	37.36187502442311	39.0	37.0	39.0	34.0	39.0
10	37.07427228456962	39.0	37.0	39.0	33.0	39.0
11	37.108864702521956	39.0	37.0	39.0	34.0	39.0
12	37.09042781833305	39.0	37.0	39.0	34.0	39.0
13	36.988665819378824	39.0	37.0	39.0	33.0	39.0
14	38.07429647507169	40.0	37.0	41.0	33.0	41.0
15	38.21037921403198	40.0	37.0	41.0	33.0	41.0
16	38.17142692328447	40.0	37.0	41.0	33.0	41.0
17	38.095961860882284	40.0	37.0	41.0	33.0	41.0
18	38.0905059722628	40.0	37.0	41.0	33.0	41.0
19	38.02397836996954	40.0	37.0	41.0	34.0	41.0
20	37.98529403555259	40.0	37.0	41.0	33.0	41.0
21	37.88551379695981	40.0	36.0	41.0	33.0	41.0
22	37.92563281422997	40.0	37.0	41.0	33.0	41.0
23	37.6868800020841	40.0	36.0	41.0	33.0	41.0
24	37.49458225794146	39.0	36.0	41.0	32.0	41.0
25	37.32124242418604	39.0	35.0	41.0	32.0	41.0
26	37.54966589195033	39.0	36.0	41.0	33.0	41.0
27	37.639909490306124	40.0	36.0	41.0	33.0	41.0
28	37.58411130608242	40.0	36.0	41.0	33.0	41.0
29	37.57119171717209	40.0	36.0	41.0	33.0	41.0
30	37.40299515631716	40.0	36.0	41.0	33.0	41.0
31	37.442088868461354	40.0	36.0	41.0	33.0	41.0
32	37.32923273309875	40.0	36.0	41.0	33.0	41.0
33	37.23154404253062	40.0	36.0	41.0	32.0	41.0
34	37.11813338642838	40.0	35.0	41.0	32.0	41.0
35	37.02329731429603	39.0	35.0	41.0	32.0	41.0
36	36.90722942458239	39.0	35.0	41.0	31.0	41.0
37	36.832488216434285	39.0	35.0	41.0	31.0	41.0
38	36.67315096175854	39.0	35.0	41.0	31.0	41.0
39	36.654446121239076	39.0	35.0	41.0	31.0	41.0
40	36.48617326726225	39.0	35.0	41.0	30.0	41.0
41	36.36816455495989	39.0	35.0	40.0	30.0	41.0
42	36.38375998556013	39.0	35.0	40.0	30.0	41.0
43	36.34606187930428	39.0	35.0	40.0	30.0	41.0
44	36.31199048755027	39.0	35.0	40.0	30.0	41.0
45	36.30090565518114	39.0	35.0	40.0	30.0	41.0
46	36.25227716360781	38.0	35.0	40.0	31.0	41.0
47	36.13869531318326	38.0	35.0	40.0	30.0	41.0
48	36.08549481671973	38.0	35.0	40.0	30.0	41.0
49	36.04012273888586	38.0	35.0	40.0	30.0	41.0
50	35.910152753716496	38.0	35.0	40.0	30.0	41.0
51	34.80782506917553	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	14.0
10	15.0
11	21.0
12	15.0
13	16.0
14	17.0
15	34.0
16	46.0
17	79.0
18	162.0
19	400.0
20	703.0
21	1178.0
22	1719.0
23	2365.0
24	2861.0
25	3466.0
26	4205.0
27	4759.0
28	5559.0
29	6436.0
30	8194.0
31	10995.0
32	14905.0
33	21425.0
34	33841.0
35	43991.0
36	50594.0
37	76553.0
38	112195.0
39	130578.0
40	55.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.88361019052812	18.30588331618289	22.555968448142078	11.25453804514692
2	31.509431504593405	29.028788558264683	22.77684634007008	16.684933597071833
3	30.841773647611376	28.959008263847668	24.68287182197279	15.516346266568167
4	27.120344026155514	23.917149391236713	30.972588439545145	17.98991814306263
5	25.661842832447277	35.858325533447086	20.50256698443062	17.97726464967501
6	24.87714016162977	37.87599948641704	22.91863989832546	14.328220453627738
7	75.71738794680323	5.196305924253956	14.165399766654696	4.9209063622881235
8	75.04284510077206	9.569762616742434	9.523614582034645	5.863777700450874
9	69.15729594846307	6.940068961538963	11.457552181704166	12.445082908293807
10	38.97778381506547	25.122394636407453	18.629663882277853	17.270157666249226
11	31.141735873211996	23.499584109445273	24.767910740768997	20.59076927657373
12	26.810519518943956	19.486379816933724	30.11643074724461	23.586669916877714
13	26.19608076650397	21.43538996019732	32.535294872916126	19.833234400382583
14	20.131894060487422	30.20984330137086	27.18249500838294	22.475767629758785
15	18.441722289314683	25.002186449225068	34.839719315743736	21.716371945716514
16	20.156642804907325	23.346253542512947	30.8683831998824	25.628720452697333
17	19.93483450905376	24.564152281071305	29.344939812170058	26.156073397704883
18	20.909711742255784	24.25656074328109	28.69049369093098	26.143233823532146
19	22.775357693789182	26.449336715041465	26.307357076000976	24.467948515168374
20	26.378812097484	24.36392935629074	27.876576336850878	21.380682209374378
21	24.001071825322246	27.13988250859228	27.41621247448367	21.4428331916018
22	20.803087452386578	22.86877024791543	28.412861159543805	27.915281140154185
23	21.794898037033796	27.65923397984001	27.050191570168273	23.495676412957923
24	23.357790551189893	24.48004376620066	26.875833874518285	25.286331808091166
25	22.546850489671584	27.49064478852849	25.287634373586947	24.674870348212973
26	20.586675499301265	26.33564135533801	27.706312418473356	25.37137072688737
27	23.93519922739258	26.041633714860968	26.059869631801952	23.9632974259445
28	21.754890668234708	25.770327930167603	28.808654989477134	23.66612641212056
29	21.686412939313474	25.504232407457373	28.157186160799853	24.6521684924293
30	23.01074988695592	27.547585508772777	27.146767497641427	22.294897106629872
31	26.384208440252248	25.67133295248799	23.168174231160716	24.77628437609904
32	24.65198241164419	27.86727229759528	23.83341303793629	23.647332252824242
33	24.535868001734272	26.833407455512738	22.999771120634314	25.63095342211868
34	25.626673564061104	25.003489014720852	25.78372574669567	23.586111674522378
35	22.8868200840713	26.454733057809715	24.527122204834008	26.131324653284977
36	25.525817778530367	27.397046153617133	24.76995762940523	22.307178438447266
37	23.493629524321687	27.94393758106144	25.748556478309492	22.813876416307377
38	24.375094203397463	27.66779369595516	23.111233510916428	24.845878589730948
39	26.191056585305944	23.0876012512072	24.473344857936624	26.24799730555023
40	23.29210403404534	25.63262814918469	29.16016159255379	21.91510622421618
41	24.000885744537133	25.78093453491899	26.81479937700153	23.403380343542345
42	22.912685313201877	27.552609689970804	27.00832339351806	22.52638160330926
43	25.278702495901573	24.045359052178913	26.401886114837897	24.27405233708162
44	24.215436889771325	24.224368767456703	27.209104560653962	24.35108978211801
45	23.076250323315364	22.62239928842708	26.34047945575092	27.960870932506637
46	25.689941030999197	25.00776887277843	25.429986174197666	23.872303922024706
47	21.48842298395425	25.160169035785195	29.892947724325037	23.458460255935513
48	22.779451471061645	24.814058775476784	26.50143933487284	25.905050418588726
49	22.64733411363209	23.59374098671197	29.283719233868194	24.475205665787744
50	22.116259552922305	22.609559714254345	29.33619401526979	25.937986717553557
51	21.515776859365726	23.0412671357143	27.011114605294743	28.431841399625235
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	507.0
1	762.0
2	1017.0
3	2009.0
4	3001.0
5	2055.5
6	1110.0
7	1072.5
8	1035.0
9	1064.0
10	1093.0
11	1147.0
12	1201.0
13	1148.0
14	1095.0
15	1117.0
16	1139.0
17	1119.5
18	1100.0
19	1034.0
20	968.0
21	1070.5
22	1173.0
23	1413.0
24	1653.0
25	1869.5
26	2752.5
27	3419.0
28	3942.0
29	4465.0
30	5606.5
31	6748.0
32	7131.0
33	7514.0
34	8823.0
35	10132.0
36	11240.5
37	12349.0
38	13245.5
39	14142.0
40	16277.5
41	18413.0
42	21280.0
43	24147.0
44	28869.5
45	33592.0
46	50299.5
47	67007.0
48	60997.0
49	54987.0
50	53672.5
51	52358.0
52	45337.5
53	38317.0
54	34297.5
55	30278.0
56	28599.0
57	26920.0
58	25504.5
59	24089.0
60	23400.0
61	22711.0
62	21497.0
63	20283.0
64	17279.0
65	14275.0
66	12163.0
67	10051.0
68	8755.5
69	7460.0
70	6529.0
71	5598.0
72	4796.5
73	3995.0
74	3500.0
75	2150.5
76	1296.0
77	1099.5
78	903.0
79	676.5
80	450.0
81	337.0
82	224.0
83	136.0
84	48.0
85	42.5
86	37.0
87	22.0
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	537401.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.4339828853248
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24628596540835	24.48915071381069
2	9.717305635492469	6.49776460617051
3	3.7116911502830705	3.722898551825272
4	2.0956225281293346	2.8026003095830903
5	1.3370342050381845	2.2351189364170256
6	0.9688391346644836	1.943529062820314
7	0.8190267467439701	1.9168328363282805
8	0.6699052023499902	1.79180792561277
9	0.5554411925609729	1.6713550193299171
>10	6.7432254031328185	41.611695786553895
>50	0.07830942006317258	1.7718355558701344
>100	0.04653168438536328	2.902972035589342
>500	0.003972216959726133	0.8949296838033413
>1k	0.005107136091076457	2.5125193560753973
>5k	0.0017023786970254858	3.234989620210043
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	6006	1.1176011953829637	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	5855	1.0895029968310443	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	5190	0.965759274731532	No Hit
GCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	2463	0.4583169737309756	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1537	0.2860061667172186	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTT	1496	0.2783768545276246	No Hit
CTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGCT	1485	0.2763299658913921	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTAGCAGAGT	1410	0.26237390700798846	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCT	1311	0.24395190928189564	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCT	1293	0.24060245514987877	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTC	1147	0.21343466052351967	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTA	1101	0.20487494440836543	No Hit
CCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTCTGC	999	0.1858947043269365	No Hit
GAATATGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCGTCTTC	690	0.1283957417273135	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGC	688	0.12802358015708942	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCCG	645	0.12002210639727132	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	586	0.10904334007566045	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	560	0.10420523966274718	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGCAGAGTCGTATGCC	549	0.10215835102651465	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2979153369643897	0.0
2	0.0	0.0	0.0	1.1466297978604432	0.0
3	0.0	0.0	0.0	1.5686610184945693	0.0
4	0.0	0.0	0.0	2.2912127070846537	0.0
5	0.0	0.0	0.0	4.501108111075342	0.0
6	0.0	0.0	0.0	5.6730448957110235	0.0
7	0.0	0.0	0.0	6.676950731390526	0.0
8	0.0	0.0	0.0	8.145872449065036	0.0
9	0.0	0.0	0.0	8.607352796142917	0.0
10	0.0	0.0	0.0	10.67954841915069	0.0
11	0.0	0.0	0.0	11.994581327537537	0.0
12	0.0	0.0	0.0	14.349619743915623	0.0
13	0.0	0.0	0.0	14.882182950906307	0.0
14	0.0	0.0	0.0	15.141021322997165	0.0
15	0.0	0.0	0.0	15.713777979572052	0.0
16	0.0	0.0	0.0	16.40134648056107	0.0
17	0.0	0.0	0.0	17.22512611625211	0.0
18	0.0	0.0	0.0	18.14417911392052	0.0
19	0.0	0.0	0.0	19.024899469855843	0.0
20	0.0	0.0	0.0	19.58500263304311	0.0
21	0.0	0.0	0.0	20.176553448914312	0.0
22	0.0	0.0	0.0	20.879938816637853	0.0
23	0.0	0.0	0.0	21.531965887670474	0.0
24	0.0	0.0	0.0	22.054108570694883	0.0
25	0.0	0.0	0.0	22.482838699593042	0.0
26	0.0	0.0	0.0	23.007958675179243	0.0
27	0.0	0.0	0.0	23.476137930521155	0.0
28	0.0	0.0	0.0	23.92980288462433	0.0
29	0.0	0.0	0.0	24.419195349469017	0.0
30	0.0	0.0	0.0	24.942454517204098	0.0
31	0.0	0.0	0.0	25.45138546448555	0.0
32	0.0	0.0	0.0	25.92905483986818	0.0
33	0.0	0.0	0.0	26.445429018554115	0.0
34	0.0	0.0	0.0	26.90821193112778	0.0
35	0.0	0.0	0.0	27.40151209245982	0.0
36	0.0	0.0	0.0	27.854990965777883	0.0
37	0.0	0.0	0.0	28.322053736409124	0.0
38	0.0	0.0	0.0	28.755063723364863	0.0
39	0.0	0.0	0.0	29.21468326259162	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTA	35	1.2103192E-7	45.000004	41
CGGTCTA	45	3.8380676E-10	45.000004	31
TGAGACG	30	2.1633368E-6	45.000004	1
GGTGTCG	25	3.888024E-5	45.0	9
CGTATAC	20	7.0299313E-4	45.0	39
GGCCGAT	50	2.1827873E-11	45.0	8
CACGCTA	20	7.0299313E-4	45.0	25
GCACGAT	20	7.0299313E-4	45.0	17
TAGCACG	20	7.0299313E-4	45.0	2
GCCGATT	20	7.0299313E-4	45.0	9
TCGCTAG	20	7.0299313E-4	45.0	1
GCGATAT	25	3.888024E-5	45.0	9
TCGTAAG	20	7.0299313E-4	45.0	1
CGTAAGG	75	0.0	45.0	2
ATTATGA	20	7.0299313E-4	45.0	40
CGTCCAG	20	7.0299313E-4	45.0	14
TGACTAG	25	3.888024E-5	45.0	1
CGAGACA	80	0.0	42.1875	22
TCAAGCG	80	0.0	42.1875	17
GGTACCC	60	3.6379788E-12	41.250004	8
>>END_MODULE