FastQCFastQC Report
Sat 18 Jun 2016
SRR3550073_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550073_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695055
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC83781.2053722367294675No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC70781.0183366783923575No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG65760.9461121781729503No Hit
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC39830.5730481760436225No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT25740.3703304055074778No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC18600.26760472192848045No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCT18480.26587823985152254No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGTACTT17880.25724582946673286No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17830.25652646193466705No Hit
GAACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCT16990.2444410873959615No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGTACTTCGTA16700.2402687557099798No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTC16080.23134859831236376No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC10270.1477580910863169No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC10000.14387350641316154No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG7800.11222133500226601No Hit
ACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC7690.11063872643172123No Hit
GAATATGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC7280.10473991266878162No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC6960.10013596046356045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACGG351.2109922E-745.0000042
CGACAGG700.045.0000042
GCGTAAG750.045.0000041
TTATCGT207.03136E-445.027
TGCGACA207.03136E-445.039
AACTACG207.03136E-445.01
TGTAACG207.03136E-445.01
TATCCGG207.03136E-445.02
GTCGAAG502.1827873E-1144.9999961
CGTAAGG950.042.631582
TAATACG2750.041.7272724
TAAGGGT1600.040.781254
CATACGA501.0804797E-940.49999618
AACGGGC900.040.04
CTACGAA403.4555887E-739.37511
TTGCGAG403.4555887E-739.3751
GCTACGA403.4555887E-739.37510
TACGGCT12350.039.3522267
CGTTCGG700.038.5714345
TATCGCG356.2447252E-638.571431