##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550073_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695055
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88320492622886	33.0	31.0	34.0	30.0	34.0
2	31.9600089201574	33.0	31.0	34.0	30.0	34.0
3	31.94608771967686	33.0	31.0	34.0	30.0	34.0
4	35.67562135370582	37.0	35.0	37.0	33.0	37.0
5	31.361590089992877	37.0	35.0	37.0	0.0	37.0
6	33.11496068656437	37.0	35.0	37.0	17.0	37.0
7	35.13681075598334	37.0	35.0	37.0	32.0	37.0
8	35.27993612016315	37.0	35.0	37.0	33.0	37.0
9	37.35901763169821	39.0	37.0	39.0	34.0	39.0
10	36.93723518282726	39.0	37.0	39.0	33.0	39.0
11	36.985791052506634	39.0	37.0	39.0	33.0	39.0
12	37.01101783312112	39.0	37.0	39.0	33.0	39.0
13	36.88358187481566	39.0	37.0	39.0	33.0	39.0
14	37.89323722583105	40.0	37.0	41.0	33.0	41.0
15	38.04048744343973	40.0	37.0	41.0	33.0	41.0
16	38.02286006143399	40.0	37.0	41.0	33.0	41.0
17	37.96003193991842	40.0	37.0	41.0	33.0	41.0
18	37.96979807353375	40.0	37.0	41.0	33.0	41.0
19	37.92835531001144	40.0	37.0	41.0	33.0	41.0
20	37.8897094474538	40.0	37.0	41.0	33.0	41.0
21	37.83915085856515	40.0	36.0	41.0	33.0	41.0
22	37.84424829689737	40.0	36.0	41.0	33.0	41.0
23	37.5561099481336	39.0	36.0	41.0	33.0	41.0
24	37.3002064584817	39.0	35.0	41.0	32.0	41.0
25	37.17780751163577	39.0	35.0	41.0	32.0	41.0
26	37.44139960147039	39.0	36.0	41.0	33.0	41.0
27	37.52896964988382	39.0	36.0	41.0	33.0	41.0
28	37.5051441972218	39.0	36.0	41.0	33.0	41.0
29	37.54331096100309	39.0	36.0	41.0	33.0	41.0
30	37.34083058175252	39.0	36.0	41.0	32.0	41.0
31	37.407800821517725	39.0	36.0	41.0	33.0	41.0
32	37.233066447978935	39.0	36.0	41.0	32.0	41.0
33	37.13798188632554	39.0	35.0	41.0	32.0	41.0
34	37.103878110365365	39.0	35.0	41.0	32.0	41.0
35	37.02665976073836	39.0	35.0	41.0	32.0	41.0
36	36.93218234528203	39.0	35.0	41.0	31.0	41.0
37	36.912204070181495	39.0	35.0	41.0	31.0	41.0
38	36.832843444043995	39.0	35.0	41.0	31.0	41.0
39	36.762237520771734	39.0	35.0	41.0	31.0	41.0
40	36.65560135528843	39.0	35.0	41.0	31.0	41.0
41	36.551455640201134	39.0	35.0	41.0	30.0	41.0
42	36.69138125759832	39.0	35.0	41.0	31.0	41.0
43	36.612409089928136	39.0	35.0	41.0	31.0	41.0
44	36.59343217443224	39.0	35.0	41.0	31.0	41.0
45	36.55574019322212	39.0	35.0	40.0	31.0	41.0
46	36.462462682809274	39.0	35.0	40.0	31.0	41.0
47	36.37392868190287	39.0	35.0	40.0	31.0	41.0
48	36.34573666832121	39.0	35.0	40.0	31.0	41.0
49	36.36181165519275	39.0	35.0	40.0	31.0	41.0
50	36.22751149189632	39.0	35.0	40.0	30.0	41.0
51	35.24888677874413	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	12.0
10	15.0
11	23.0
12	11.0
13	17.0
14	14.0
15	22.0
16	56.0
17	94.0
18	201.0
19	351.0
20	669.0
21	1370.0
22	2150.0
23	2936.0
24	3576.0
25	4498.0
26	5325.0
27	6106.0
28	6833.0
29	8134.0
30	10681.0
31	14445.0
32	19601.0
33	28181.0
34	43909.0
35	56159.0
36	66352.0
37	100925.0
38	150212.0
39	162121.0
40	54.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.59415441943444	18.92512103358727	23.1689578522563	11.311766694722
2	30.632108250426228	28.265532943436135	24.1998115257066	16.902547280431047
3	29.826560488018934	28.82289890728072	26.03923430519887	15.311306299501478
4	27.186625518843833	23.773226579191576	31.12976670910935	17.91038119285524
5	25.011258101876834	36.351799497881466	21.4713943500874	17.165548050154307
6	24.300379106689398	37.20899784909108	24.161685046507113	14.328937997712412
7	74.9257253023142	5.596823272978398	14.374545899245383	5.102905525462014
8	73.9614850623332	9.02130047262447	10.423203919114314	6.59401054592802
9	68.3484040831301	6.843487206048442	12.350821158037853	12.457287552783592
10	37.668817575587546	23.62546848810526	21.05761414564315	17.648099790664048
11	29.932163641726195	23.511664544532447	26.06829675349433	20.487875060247028
12	24.75372452539727	20.48744343972779	31.463840991000712	23.294991043874226
13	24.744228873974002	21.984447273956736	34.440152218169786	18.831171633899476
14	20.79950507513794	29.042018257547962	28.645646747379704	21.512829919934394
15	18.44544676320579	23.75567401140917	36.68587377977283	21.113005445612217
16	19.885332815388708	24.320665271093656	30.129989713044292	25.664012200473348
17	20.244441087395963	24.664810698433936	30.433850558588887	24.656897655581215
18	20.32716835358353	24.450726920891153	30.814252109545286	24.40785261598003
19	20.952298738948716	26.38381135305839	28.340059419758152	24.323830488234744
20	23.371387875779615	25.525605887303882	30.397018940947117	20.705987295969383
21	21.80028918574789	28.61370682895598	28.705066505528336	20.880937479767788
22	19.512412686765796	24.17204393896886	29.51651308169857	26.799030292566776
23	20.4831272345354	27.19194883858112	28.387393803368077	23.937530123515405
24	21.79223226938875	25.163332398155543	27.886570127543862	25.15786520491184
25	19.514139168842753	29.491047471063442	26.00758213378797	24.98723122630583
26	18.85404752141917	25.87651336944558	29.320557365963847	25.948881743171405
27	22.337512858694637	26.744214486623363	26.848810525785726	24.069462128896273
28	18.26805072979836	26.253749703260894	29.163591370467085	26.31460819647366
29	21.38032242052787	23.471379962736762	27.314816813057956	27.833480803677407
30	20.993446561782882	28.232873657480344	27.450345656099156	23.32333412463762
31	21.80057693276072	25.941831941357158	23.440447158857932	28.817143967024194
32	24.16657674572516	27.216695081684183	23.5997151304573	25.017013042133357
33	20.91158253663379	25.53366280366302	26.78924689413068	26.76550776557251
34	21.026537468257906	25.545316557682483	25.628043823870055	27.800102150189552
35	20.629590464063995	24.567408334592226	25.92111415643366	28.881887044910115
36	19.037486242095948	25.603297580766988	28.8270712389667	26.53214493817036
37	20.461114588054183	27.427182021566637	27.825567760824683	24.286135629554494
38	20.375653725244767	24.328578313946377	27.16058441418305	28.135183546625807
39	23.55770406658466	24.280668436310794	29.199272000057547	22.962355497046996
40	19.970793678198127	23.645754652509513	31.379243369229776	25.004208300062587
41	18.335959024825375	26.699182079116042	27.69334800843099	27.2715108876276
42	20.574055290588515	23.212983145218725	29.504571580666276	26.70838998352648
43	21.90977692412831	22.882649574494103	29.135248289703693	26.072325211673895
44	21.579731100416513	23.688053463394983	28.469113955010755	26.26310148117775
45	21.02941493838617	22.02415636172677	28.062383552380748	28.88404514750631
46	24.679198049075254	23.884872420168186	27.617238923538423	23.818690607218134
47	18.38458826999302	23.679564926516605	33.011200552474264	24.924646251016107
48	19.987195257929226	24.01737991957471	28.370560603117738	27.62486421937832
49	20.938918502852292	20.94381020207034	32.74719266820612	25.370078626871255
50	20.82698491486285	20.459819726496463	31.270762745394247	27.44243261324643
51	19.783614246354606	21.444777751400967	27.80211637927934	30.96949162296509
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	324.0
1	605.5
2	887.0
3	2162.0
4	3437.0
5	2382.5
6	1328.0
7	1300.5
8	1273.0
9	1323.5
10	1374.0
11	1380.0
12	1386.0
13	1427.5
14	1469.0
15	1470.0
16	1471.0
17	1503.5
18	1536.0
19	1578.5
20	1621.0
21	1795.5
22	1970.0
23	2155.5
24	2341.0
25	2623.5
26	3737.0
27	4568.0
28	5402.5
29	6237.0
30	7336.0
31	8435.0
32	9403.0
33	10371.0
34	11750.0
35	13129.0
36	15006.0
37	16883.0
38	18983.5
39	21084.0
40	23938.0
41	26792.0
42	30012.0
43	33232.0
44	40398.5
45	47565.0
46	70034.0
47	92503.0
48	87457.0
49	82411.0
50	79697.5
51	76984.0
52	65797.5
53	54611.0
54	47076.5
55	39542.0
56	36054.5
57	32567.0
58	29446.0
59	26325.0
60	24289.5
61	22254.0
62	20083.5
63	17913.0
64	15246.5
65	12580.0
66	10297.0
67	8014.0
68	6929.5
69	5845.0
70	5079.0
71	4313.0
72	3724.5
73	3136.0
74	2524.5
75	1543.0
76	1173.0
77	925.0
78	677.0
79	540.0
80	403.0
81	265.5
82	128.0
83	99.0
84	70.0
85	59.0
86	48.0
87	32.5
88	17.0
89	11.5
90	6.0
91	4.0
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.91651111300181
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.22022911474649	24.494582033538357
2	11.013763275237876	7.470968490411532
3	4.296549878680651	4.371719451235165
4	2.202961769195786	2.9886710930578797
5	1.4011152440633508	2.376047037293544
6	0.9969017218628118	2.0286856996878417
7	0.7490749081645354	1.7784205213063293
8	0.6114507700220104	1.6590621469204023
9	0.5143311538259815	1.5699886465051751
>10	5.855114310511914	39.37402343281543
>50	0.08050768997344286	1.8116985840621653
>100	0.04934342286219926	3.1782897924960056
>500	0.003029859298556095	0.7508984890816198
>1k	0.0043283704265087075	2.9125759576499197
>5k	0.001298511127952612	3.2343686239386606
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC	8378	1.2053722367294675	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC	7078	1.0183366783923575	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG	6576	0.9461121781729503	No Hit
GCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	3983	0.5730481760436225	No Hit
CTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGCT	2574	0.3703304055074778	No Hit
CCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	1860	0.26760472192848045	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCT	1848	0.26587823985152254	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACGTACTT	1788	0.25724582946673286	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1783	0.25652646193466705	No Hit
GAACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCT	1699	0.2444410873959615	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACGTACTTCGTA	1670	0.2402687557099798	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTC	1608	0.23134859831236376	No Hit
TCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	1027	0.1477580910863169	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGC	1000	0.14387350641316154	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCG	780	0.11222133500226601	No Hit
ACTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTCTGC	769	0.11063872643172123	No Hit
GAATATGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCCGTCTTC	728	0.10473991266878162	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCACGTACTTCGTATGCC	696	0.10013596046356045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4387350641316155E-4	0.0	0.0	0.3933501665335837	0.0
2	1.4387350641316155E-4	0.0	0.0	1.557574580428887	0.0
3	2.877470128263231E-4	0.0	0.0	2.1324931120558803	0.0
4	2.877470128263231E-4	0.0	0.0	3.064505686600341	0.0
5	2.877470128263231E-4	0.0	0.0	5.650775837883333	0.0
6	2.877470128263231E-4	0.0	0.0	6.771406579335448	0.0
7	2.877470128263231E-4	0.0	0.0	8.183812791793455	0.0
8	2.877470128263231E-4	0.0	0.0	10.295012624900188	0.0
9	2.877470128263231E-4	0.0	0.0	10.994813360093806	0.0
10	2.877470128263231E-4	0.0	0.0	13.516484306997288	0.0
11	2.877470128263231E-4	0.0	0.0	15.354756098438253	0.0
12	2.877470128263231E-4	0.0	0.0	18.278697369272937	0.0
13	2.877470128263231E-4	0.0	0.0	18.96655660343426	0.0
14	2.877470128263231E-4	0.0	0.0	19.287538396242024	0.0
15	2.877470128263231E-4	0.0	0.0	20.01870355583371	0.0
16	2.877470128263231E-4	0.0	0.0	21.02121414852062	0.0
17	2.877470128263231E-4	0.0	0.0	22.17378480839646	0.0
18	2.877470128263231E-4	0.0	0.0	23.356425031112646	0.0
19	2.877470128263231E-4	0.0	0.0	24.451158541410393	0.0
20	2.877470128263231E-4	0.0	0.0	25.22649286747092	0.0
21	2.877470128263231E-4	0.0	0.0	26.060959204667256	0.0
22	2.877470128263231E-4	0.0	0.0	26.930242930415577	0.0
23	2.877470128263231E-4	0.0	0.0	27.74384760918201	0.0
24	2.877470128263231E-4	0.0	0.0	28.421779571400826	0.0
25	2.877470128263231E-4	0.0	0.0	28.982310752386503	0.0
26	2.877470128263231E-4	0.0	0.0	29.523994504032053	0.0
27	2.877470128263231E-4	0.0	0.0	30.077763630216314	0.0
28	2.877470128263231E-4	0.0	0.0	30.630237894842853	0.0
29	2.877470128263231E-4	0.0	0.0	31.216378559970074	0.0
30	2.877470128263231E-4	0.0	0.0	31.83460301702743	0.0
31	2.877470128263231E-4	0.0	0.0	32.42534763435987	0.0
32	2.877470128263231E-4	0.0	0.0	32.97897288703771	0.0
33	2.877470128263231E-4	0.0	0.0	33.496629763112274	0.0
34	2.877470128263231E-4	0.0	0.0	34.015725374250955	0.0
35	2.877470128263231E-4	0.0	0.0	34.53381387084475	0.0
36	2.877470128263231E-4	0.0	0.0	35.0470106682205	0.0
37	2.877470128263231E-4	0.0	0.0	35.568552128968214	0.0
38	2.877470128263231E-4	0.0	0.0	36.065922840638514	0.0
39	2.877470128263231E-4	0.0	0.0	36.56890461905893	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACGG	35	1.2109922E-7	45.000004	2
CGACAGG	70	0.0	45.000004	2
GCGTAAG	75	0.0	45.000004	1
TTATCGT	20	7.03136E-4	45.0	27
TGCGACA	20	7.03136E-4	45.0	39
AACTACG	20	7.03136E-4	45.0	1
TGTAACG	20	7.03136E-4	45.0	1
TATCCGG	20	7.03136E-4	45.0	2
GTCGAAG	50	2.1827873E-11	44.999996	1
CGTAAGG	95	0.0	42.63158	2
TAATACG	275	0.0	41.727272	4
TAAGGGT	160	0.0	40.78125	4
CATACGA	50	1.0804797E-9	40.499996	18
AACGGGC	90	0.0	40.0	4
CTACGAA	40	3.4555887E-7	39.375	11
TTGCGAG	40	3.4555887E-7	39.375	1
GCTACGA	40	3.4555887E-7	39.375	10
TACGGCT	1235	0.0	39.352226	7
CGTTCGG	70	0.0	38.57143	45
TATCGCG	35	6.2447252E-6	38.57143	1
>>END_MODULE