##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550069_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	673708
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.905374435215258	33.0	31.0	34.0	30.0	34.0
2	32.003247697815674	33.0	31.0	34.0	30.0	34.0
3	31.981625570722034	33.0	31.0	34.0	30.0	34.0
4	35.694085568228374	37.0	35.0	37.0	33.0	37.0
5	32.17083217061398	37.0	35.0	37.0	16.0	37.0
6	33.53760828133257	37.0	35.0	37.0	17.0	37.0
7	35.260850398095315	37.0	35.0	37.0	32.0	37.0
8	35.332794029460835	37.0	35.0	37.0	33.0	37.0
9	37.34206065535811	39.0	37.0	39.0	34.0	39.0
10	37.00530051595053	39.0	37.0	39.0	33.0	39.0
11	37.014489957073394	39.0	37.0	39.0	33.0	39.0
12	37.012109103647276	39.0	37.0	39.0	33.0	39.0
13	36.9077226335445	39.0	37.0	39.0	33.0	39.0
14	37.93352609735969	40.0	37.0	41.0	33.0	41.0
15	38.075572800085496	40.0	37.0	41.0	33.0	41.0
16	38.02516372078111	40.0	37.0	41.0	33.0	41.0
17	37.981198085817596	40.0	37.0	41.0	33.0	41.0
18	37.97027050294786	40.0	37.0	41.0	33.0	41.0
19	37.93180131451608	40.0	37.0	41.0	33.0	41.0
20	37.89998337558705	40.0	36.0	41.0	33.0	41.0
21	37.83126963016618	40.0	36.0	41.0	33.0	41.0
22	37.810205608364456	40.0	36.0	41.0	33.0	41.0
23	37.57361646291865	39.0	36.0	41.0	33.0	41.0
24	37.347623896406155	39.0	35.0	41.0	32.0	41.0
25	37.1968968752041	39.0	35.0	41.0	32.0	41.0
26	37.47488229321902	39.0	36.0	41.0	33.0	41.0
27	37.5499073782707	39.0	36.0	41.0	33.0	41.0
28	37.43475214781478	39.0	36.0	41.0	33.0	41.0
29	37.47043823139995	39.0	36.0	41.0	33.0	41.0
30	37.30372802460413	39.0	35.0	41.0	32.0	41.0
31	37.24156014178249	39.0	35.0	41.0	32.0	41.0
32	37.14502870679879	39.0	35.0	41.0	32.0	41.0
33	37.099550547121304	39.0	35.0	41.0	32.0	41.0
34	36.97038924875466	39.0	35.0	41.0	31.0	41.0
35	36.933472661746634	39.0	35.0	41.0	31.0	41.0
36	36.77302629625891	39.0	35.0	41.0	31.0	41.0
37	36.74352390056226	39.0	35.0	41.0	31.0	41.0
38	36.564308275989006	39.0	35.0	41.0	31.0	41.0
39	36.54754136807044	39.0	35.0	41.0	31.0	41.0
40	36.49780617121958	39.0	35.0	41.0	31.0	41.0
41	36.345248980270384	39.0	35.0	40.0	30.0	41.0
42	36.40014219810363	39.0	35.0	40.0	31.0	41.0
43	36.29377415735007	39.0	35.0	40.0	30.0	41.0
44	36.24531547792218	39.0	35.0	40.0	30.0	41.0
45	36.13944171658938	38.0	35.0	40.0	30.0	41.0
46	36.04992222149655	38.0	35.0	40.0	30.0	41.0
47	35.940230782475496	38.0	35.0	40.0	30.0	41.0
48	35.8902729372369	38.0	35.0	40.0	30.0	41.0
49	35.859590505085286	38.0	35.0	40.0	30.0	41.0
50	35.71414321931757	38.0	35.0	40.0	29.0	41.0
51	34.631456061082844	36.0	33.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	12.0
10	10.0
11	14.0
12	9.0
13	20.0
14	27.0
15	30.0
16	56.0
17	107.0
18	203.0
19	394.0
20	779.0
21	1289.0
22	2024.0
23	2721.0
24	3478.0
25	4352.0
26	5317.0
27	6478.0
28	7350.0
29	8740.0
30	11109.0
31	14815.0
32	19563.0
33	27983.0
34	45218.0
35	56213.0
36	63679.0
37	95581.0
38	142336.0
39	153746.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.43469277491139	19.34102014522612	23.931139306643235	13.293147773219257
2	30.84184839722847	27.736645549704026	24.519821643798203	16.901684409269297
3	30.318921550582743	27.167259406152223	26.26983797134664	16.243981071918398
4	27.02550659929821	24.31528199160467	30.49807928657519	18.161132122521924
5	25.31794189767674	34.50456280762585	22.370374108664286	17.807121186033118
6	25.32373075575769	35.55516633318886	24.11727335878452	15.003829552268936
7	75.91360055098055	5.676791725792183	13.06515582418496	5.344451899042315
8	75.0630837098565	8.317253171997365	10.139259144911446	6.480403973234695
9	68.78113366621741	7.196441188170542	12.58943043573774	11.432994709874308
10	40.176901565663464	23.168345930284335	19.616807281492875	17.03794522255933
11	30.44212032512602	23.268686137020786	25.5768077564761	20.712385781377098
12	25.672694995457974	19.277639570852653	31.510238857190352	23.539426576499018
13	25.16253332304203	21.224477073153356	33.79461131528793	19.818378288516687
14	20.056166766611053	27.085176367209534	29.833696497592427	23.02496036858698
15	18.152819916046713	23.048412665427755	36.68562641381726	22.113141004708268
16	20.382569303021487	23.585589008888125	30.697275377463235	25.334566310627153
17	20.831280020424277	24.34482001104336	29.498388025672845	25.325511942859517
18	21.91572610092206	23.18155640128958	30.23416673098731	24.668550766801047
19	22.582632238299087	24.997328219347253	27.391540548724375	25.028498993629288
20	24.112078229737513	24.590030102062023	28.879870804562213	22.41802086363825
21	23.363237485676287	27.15627541902427	28.047017402197984	21.43346969310146
22	20.75572800085497	23.827385157961608	28.142014047628944	27.27487279355448
23	21.537966003075518	25.921022163904837	27.74050478842466	24.800507044594987
24	22.82635800673289	23.122777226929177	27.96716084713259	26.08370391920535
25	21.336246563793214	26.83432585036841	25.953825693030215	25.87560189280816
26	21.925522629982126	24.291087533471476	27.917139176022847	25.866250660523548
27	24.56405445682699	24.294056178641192	26.986172050799457	24.155717313732357
28	21.42085295113016	24.57860081815861	29.94041929144377	24.060126939267455
29	24.898620767454148	22.438356083050817	27.592517826714246	25.07050532278079
30	26.799444269624228	24.017823745598985	27.13623706412867	22.046494920648115
31	25.141604374595524	23.083887975205876	27.014671044428745	24.75983660576986
32	26.7188455532664	24.109406449084766	26.631567385276707	22.540180612372126
33	26.3563739780439	22.1422337273715	26.930955250642715	24.570437043941887
34	24.604576463393638	21.289490402370166	30.585209022306397	23.5207241119298
35	25.24595225231109	21.30448206047724	28.06764948612752	25.38191620108415
36	26.16682598395744	22.828436058351688	29.369994122082566	21.634743835608305
37	23.287388601590006	22.938275929631235	32.996045764633934	20.77828970414482
38	23.213172472347072	23.060138813848134	29.318191263870997	24.4084974499338
39	24.126030862035183	21.699905597083603	31.746246148182895	22.42781739269832
40	24.64435630866785	20.85710723340082	30.82611457782897	23.67242188010236
41	21.230859660268248	22.055846152932723	30.162177085621668	26.55111710117736
42	22.566898418899584	21.6820937260653	30.81928669393862	24.931721161096497
43	24.400333675717075	20.855919775332936	30.566803422254154	24.17694312669584
44	23.88349255166927	21.803362881248255	29.593681535620775	24.719463031461704
45	23.534825176485956	20.032417605253315	29.3118086767561	27.12094854150463
46	26.018987454505517	21.898953255713156	28.520813171284885	23.56124611849644
47	21.682835887357726	21.289490402370166	33.206522707166904	23.821151003105204
48	22.133921520896294	21.45573453187434	30.43588617026961	25.974457776959753
49	22.87697340687657	19.677664507472077	33.11731491981689	24.32804716583446
50	21.94674844294561	19.935788204979012	31.566049386381046	26.551413965694337
51	21.778129397305655	19.988926953516952	28.786952210750055	29.44599143842733
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	626.0
2	1012.0
3	2616.5
4	4221.0
5	2794.0
6	1367.0
7	1324.0
8	1281.0
9	1319.0
10	1357.0
11	1428.0
12	1499.0
13	1479.5
14	1460.0
15	1436.0
16	1412.0
17	1372.5
18	1333.0
19	1422.5
20	1512.0
21	1626.5
22	1741.0
23	2123.0
24	2505.0
25	2900.0
26	4023.0
27	4751.0
28	5842.0
29	6933.0
30	7855.5
31	8778.0
32	9367.5
33	9957.0
34	10879.5
35	11802.0
36	13137.0
37	14472.0
38	15441.0
39	16410.0
40	19219.5
41	22029.0
42	25014.0
43	27999.0
44	34561.5
45	41124.0
46	58309.0
47	75494.0
48	74209.0
49	72924.0
50	70578.5
51	68233.0
52	59278.5
53	50324.0
54	45206.0
55	40088.0
56	36865.5
57	33643.0
58	32861.5
59	32080.0
60	30262.0
61	28444.0
62	26388.0
63	24332.0
64	21109.5
65	17887.0
66	15563.5
67	13240.0
68	11316.5
69	9393.0
70	8124.0
71	6855.0
72	5917.5
73	4980.0
74	4040.0
75	2523.5
76	1947.0
77	1544.0
78	1141.0
79	926.0
80	711.0
81	485.5
82	260.0
83	173.0
84	86.0
85	56.5
86	27.0
87	23.5
88	20.0
89	12.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	673708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.43000435186555
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.19539033707986	27.39703778853396
2	9.76081500252071	7.306946960442093
3	3.763005465578615	4.2254793285810415
4	2.048499562697006	3.067013901861744
5	1.4532035653720585	2.7196707888011336
6	1.1757235761232165	2.6404403142529747
7	0.9824430520887334	2.574099340059895
8	0.8587882686235935	2.571555890552974
9	0.7713060194841145	2.598298889931945
>10	5.891470324980521	35.30497098787777
>50	0.05210070124189756	1.3504268665555914
>100	0.03998425894666448	3.0140838228727134
>500	0.00282716982451163	0.7220014213080589
>1k	0.003634932631514953	2.9132515473257126
>5k	8.077628070033228E-4	1.5947221510424443
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	5542	0.8226115765287038	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	5007	0.7432003182387622	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	4820	0.7154434859019041	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2791	0.41427443343406933	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	2353	0.3492611042172573	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2222	0.32981647835560807	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	2095	0.31096558152790227	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTTGTCTT	1503	0.22309368450426592	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCT	1213	0.180048329543363	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCT	1184	0.1757437940472727	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTA	1090	0.1617911617496007	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTC	969	0.14383085847281019	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCC	782	0.11607402613595208	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	758	0.11251165193229115	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTG	718	0.10657436159285626	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.484322584858722E-4	0.0	0.0	0.3707837816977088	0.0
2	1.484322584858722E-4	0.0	0.0	1.3473196102762621	0.0
3	2.968645169717444E-4	0.0	0.0	1.8941440505382154	0.0
4	2.968645169717444E-4	0.0	0.0	2.685733285043372	0.0
5	2.968645169717444E-4	0.0	0.0	4.827759207252994	0.0
6	2.968645169717444E-4	0.0	0.0	6.002897397685644	0.0
7	2.968645169717444E-4	0.0	0.0	7.291289401343015	0.0
8	2.968645169717444E-4	0.0	0.0	9.355239955589068	0.0
9	2.968645169717444E-4	0.0	0.0	10.170875215968936	0.0
10	2.968645169717444E-4	0.0	0.0	12.392609260985472	0.0
11	2.968645169717444E-4	0.0	0.0	14.113384433612188	0.0
12	2.968645169717444E-4	0.0	0.0	16.466332595130236	0.0
13	2.968645169717444E-4	0.0	0.0	17.140511913173064	0.0
14	2.968645169717444E-4	0.0	0.0	17.44746982372185	0.0
15	2.968645169717444E-4	0.0	0.0	18.06019818675153	0.0
16	2.968645169717444E-4	0.0	0.0	18.932683002131487	0.0
17	2.968645169717444E-4	0.0	0.0	19.920796546871937	0.0
18	2.968645169717444E-4	0.0	0.0	20.9728843950198	0.0
19	2.968645169717444E-4	0.0	0.0	21.855611036235285	0.0
20	2.968645169717444E-4	0.0	0.0	22.549531844656734	0.0
21	2.968645169717444E-4	0.0	0.0	23.268686137020786	0.0
22	2.968645169717444E-4	0.0	0.0	24.019604932700815	0.0
23	2.968645169717444E-4	0.0	0.0	24.70981493466012	0.0
24	2.968645169717444E-4	0.0	0.0	25.27860734917798	0.0
25	2.968645169717444E-4	0.0	0.0	25.784464486097836	0.0
26	2.968645169717444E-4	0.0	0.0	26.271470726189982	0.0
27	2.968645169717444E-4	0.0	0.0	26.810428256752182	0.0011874580678869777
28	2.968645169717444E-4	0.0	0.0	27.294465851674612	0.0011874580678869777
29	2.968645169717444E-4	0.0	0.0	27.816798969286396	0.0011874580678869777
30	2.968645169717444E-4	0.0	0.0	28.401918932237706	0.0011874580678869777
31	2.968645169717444E-4	0.0	0.0	28.90807293367453	0.0011874580678869777
32	2.968645169717444E-4	0.0	0.0	29.419570496416846	0.0011874580678869777
33	2.968645169717444E-4	0.0	0.0	29.89470215583012	0.0011874580678869777
34	2.968645169717444E-4	0.0	0.0	30.394473570152055	0.0011874580678869777
35	2.968645169717444E-4	0.0	0.0	30.8920185005967	0.0011874580678869777
36	2.968645169717444E-4	0.0	0.0	31.345182185754066	0.0011874580678869777
37	2.968645169717444E-4	0.0	0.0	31.800720787047208	0.0011874580678869777
38	2.968645169717444E-4	0.0	0.0	32.245572265729365	0.0011874580678869777
39	2.968645169717444E-4	0.0	0.0	32.749499783288904	0.0011874580678869777
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	25	3.8890816E-5	45.0	1
AGTTACG	25	3.8890816E-5	45.0	1
TATAGCG	20	7.0312055E-4	45.0	1
AGTACGG	90	0.0	42.5	2
TTAACGG	65	0.0	41.53846	2
TAATACG	170	0.0	41.02941	4
GACCTAT	50	1.0804797E-9	40.5	33
ACACGTG	145	0.0	40.344826	42
CGTAAGG	85	0.0	39.705883	2
ACTGCGT	40	3.4553887E-7	39.375	32
ACGGGTA	80	0.0	39.375	5
TTTACGG	35	6.2444396E-6	38.571426	2
ATAGCGG	70	0.0	38.571426	2
CTAACGG	35	6.2444396E-6	38.571426	2
GTCAAGC	165	0.0	38.181816	16
CAAGGGA	410	0.0	37.865852	4
AACGGGC	125	0.0	37.8	4
AATACGG	185	0.0	37.7027	5
AACACGT	150	0.0	37.5	41
CGACGAA	30	1.1394206E-4	37.499996	19
>>END_MODULE