FastQCFastQC Report
Sat 18 Jun 2016
SRR3550066_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550066_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427239
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC63221.4797338257977384No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG57211.3390631473250334No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC52071.2187557783816552No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC24480.5729813991700197TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT16420.38432820973740695TruSeq Adapter, Index 23 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT15210.35600682521960775No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT13920.3258129524692268No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC13630.31902518262611795TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC13250.31013086352135455No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA12090.282979784148919No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12070.28251166209077355No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT10490.2455300194972837No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC6190.14488377699601393No Hit
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC5940.1390322512691959TruSeq Adapter, Index 20 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG5790.13552133583310513No Hit
GAATATGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC4890.1144558432165603No Hit
ACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC4730.11071086675139676TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC4710.11024274469325132No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTAC207.028303E-445.00000431
GCGAAAG207.028303E-445.0000041
TTCCGTA207.028303E-445.00000424
CACGAAG207.028303E-445.0000041
AGTATGC207.028303E-445.00000442
ACTACCA207.028303E-445.00000418
GAACGTC207.028303E-445.00000412
AATTCGC207.028303E-445.00000413
ACGTCCC207.028303E-445.00000414
TTCGAAT406.7975634E-945.00000443
CAGGTAC253.886676E-545.05
GTGCAAG453.8380676E-1045.01
CGGCGAA253.886676E-545.031
CTCACGA253.886676E-545.024
TACGGGT253.886676E-545.04
AGTTGCG253.886676E-545.01
CGGTCTA253.886676E-545.031
ATACGAG253.886676E-545.01
TGCGTAG253.886676E-545.01
CTTACGG502.1827873E-1145.02
Produced by FastQC (version 0.11.5)