##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550066_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	427239
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09185959146988	33.0	31.0	34.0	30.0	34.0
2	32.193886794042676	33.0	31.0	34.0	30.0	34.0
3	32.17971673934262	33.0	31.0	34.0	30.0	34.0
4	35.88051886648925	37.0	35.0	37.0	35.0	37.0
5	31.69175332776268	37.0	35.0	37.0	0.0	37.0
6	33.37836199410634	37.0	35.0	37.0	17.0	37.0
7	35.270670514629984	37.0	35.0	37.0	32.0	37.0
8	35.39413068563497	37.0	35.0	37.0	33.0	37.0
9	37.490657454024564	39.0	37.0	39.0	35.0	39.0
10	37.21580426880505	39.0	37.0	39.0	34.0	39.0
11	37.236263543356294	39.0	37.0	39.0	34.0	39.0
12	37.20703634265598	39.0	37.0	39.0	34.0	39.0
13	37.043511945304616	39.0	37.0	39.0	33.0	39.0
14	38.11002740854651	40.0	37.0	41.0	33.0	41.0
15	38.219474345740906	40.0	37.0	41.0	33.0	41.0
16	38.22987367726261	40.0	37.0	41.0	34.0	41.0
17	38.161143996685695	40.0	37.0	41.0	34.0	41.0
18	38.163784205093634	40.0	37.0	41.0	34.0	41.0
19	38.13166401007398	40.0	37.0	41.0	34.0	41.0
20	38.05466495333993	40.0	37.0	41.0	34.0	41.0
21	38.065635393772574	40.0	37.0	41.0	34.0	41.0
22	38.061909142189734	40.0	37.0	41.0	34.0	41.0
23	37.799053925320486	40.0	36.0	41.0	33.0	41.0
24	37.571977277355295	39.0	36.0	41.0	33.0	41.0
25	37.45674435152222	39.0	36.0	41.0	33.0	41.0
26	37.72614625537463	39.0	36.0	41.0	33.0	41.0
27	37.80710562472059	40.0	36.0	41.0	33.0	41.0
28	37.759256528547255	40.0	36.0	41.0	33.0	41.0
29	37.80471118039318	40.0	36.0	41.0	33.0	41.0
30	37.682493873452565	40.0	36.0	41.0	33.0	41.0
31	37.7085074162237	40.0	36.0	41.0	33.0	41.0
32	37.55750528392773	40.0	36.0	41.0	33.0	41.0
33	37.48749528952179	40.0	36.0	41.0	33.0	41.0
34	37.40395656763545	40.0	36.0	41.0	33.0	41.0
35	37.366197842425436	40.0	36.0	41.0	33.0	41.0
36	37.281804797782975	40.0	36.0	41.0	33.0	41.0
37	37.26747324097285	40.0	36.0	41.0	33.0	41.0
38	37.16823136464602	40.0	35.0	41.0	32.0	41.0
39	37.0279422056507	39.0	35.0	41.0	32.0	41.0
40	36.99499811580871	39.0	35.0	41.0	32.0	41.0
41	36.81331058260131	39.0	35.0	41.0	31.0	41.0
42	36.8949697944242	39.0	35.0	41.0	32.0	41.0
43	36.80611554656761	39.0	35.0	41.0	32.0	41.0
44	36.79329368339501	39.0	35.0	41.0	32.0	41.0
45	36.742806719424024	39.0	35.0	41.0	32.0	41.0
46	36.63086001043912	39.0	35.0	40.0	31.0	41.0
47	36.54784792586819	39.0	35.0	40.0	31.0	41.0
48	36.48459293276129	39.0	35.0	40.0	31.0	41.0
49	36.512062335133265	39.0	35.0	40.0	31.0	41.0
50	36.402547520240425	39.0	35.0	40.0	31.0	41.0
51	35.35568382099949	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	7.0
11	8.0
12	12.0
13	7.0
14	10.0
15	16.0
16	17.0
17	44.0
18	86.0
19	183.0
20	355.0
21	672.0
22	1176.0
23	1654.0
24	2007.0
25	2393.0
26	2863.0
27	3111.0
28	3366.0
29	4060.0
30	5267.0
31	7425.0
32	10259.0
33	15848.0
34	26718.0
35	34818.0
36	38888.0
37	61027.0
38	95550.0
39	109341.0
40	44.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.87909577543249	19.800392754406783	23.799559497143285	11.520951973017445
2	29.97432350511072	30.77715283483015	22.83195120295666	16.416572457102465
3	29.30795175534069	29.83201439943451	25.427453954344053	15.43257989088075
4	27.071264561521772	23.023647185767217	32.132366193161204	17.772722059549807
5	26.136892933463475	35.594362874175815	20.625457881888124	17.643286310472593
6	23.88873674921999	37.80483523273858	24.33883610812683	13.967591909914592
7	73.49048190825276	5.568311881640018	15.421579022514331	5.519627187592893
8	71.94427475019836	10.333092250473388	10.709462385222322	7.013170614105922
9	66.1585201725498	6.942718244355032	12.913146973941986	13.98561460915319
10	39.85240111506674	22.478050926998705	20.209297372196826	17.460250585737725
11	31.1268400122648	21.94041274321867	25.6690049363471	21.26374230816943
12	25.165305601782613	19.497751843815756	31.73282401653407	23.604118537867564
13	23.89201360362701	21.941114926305886	35.19528881960682	18.97158265046028
14	19.650593695800243	29.162365795257454	29.02333354398826	22.16370696495404
15	16.870182731445396	23.472342178499623	38.690054044691614	20.96742104536337
16	18.9748595048673	24.039472051942823	30.617757274031632	26.367911169158248
17	19.31729079040069	25.353256608128007	29.695088697426968	25.63436390404434
18	20.33779687715775	24.25948941927118	30.708807014340923	24.69390668923015
19	20.54517494891618	26.074866760759203	28.10745273722671	25.27250555309791
20	22.854187000718568	25.44524259255358	30.467490093366944	21.233080313360905
21	21.58861901652237	29.08910469315769	28.21839766500717	21.103878625312763
22	18.982115396768553	23.955444142505716	28.77054763258972	28.291892828136007
23	20.698016800900668	27.40409934486318	28.27129545757761	23.626588396658548
24	21.955860771137466	23.75579008470669	27.92722574484071	26.36112339931514
25	19.478792900460867	29.28220504214269	26.0671427467998	25.171859310596645
26	18.64764218622364	24.949969455035706	30.139804652665138	26.26258370607552
27	22.658511980413774	25.211415624509936	26.549074405660534	25.58099798941576
28	18.17647733470025	24.89356074702918	30.234365308410517	26.695596609860058
29	22.317719122083894	21.59212993195846	29.004140539604293	27.08601040635335
30	21.22137726190727	24.130521792252114	27.38514040150829	27.262960544332334
31	22.57659062023832	23.125463733413852	24.926563352128433	29.371382294219394
32	25.427453954344053	24.50618974391383	25.631087049637323	24.435269252104792
33	21.228867214837596	21.78663464711789	25.3944513492448	31.590046788799715
34	20.75676611919792	20.643714642155793	31.76395413340074	26.83556510524554
35	20.889712783711225	20.455295513752255	30.13676185928719	28.518229843249326
36	19.60822864953808	22.035675582051265	31.323685337714956	27.0324104306957
37	21.34262087496694	22.24679863027486	31.434162143437277	24.976418351320923
38	20.276941009598843	22.737156486182208	27.326625144240108	29.65927735997884
39	24.238189865625564	21.424308174113317	30.87405410086626	23.463447859394858
40	20.085010965759214	20.234810024365753	33.784134875327396	25.89604413454764
41	18.508375873925367	22.297355812554564	32.5665494020911	26.627718911428964
42	20.73710499275581	20.854369568321243	34.006024730888335	24.402500708034612
43	22.330826539711964	20.18191223179532	30.999510812449238	26.487750416043482
44	21.839532439688327	20.62428757674276	29.840674657510196	27.69550532605872
45	21.40394486458399	18.811016784516397	29.33135785824796	30.453680492651653
46	24.879048963226673	21.625834720144933	29.32971943104445	24.16539688558395
47	18.791355658074284	20.367288566820914	35.659899962316175	25.181455812788627
48	20.044986529787774	21.34683397349025	31.09547583436905	27.512703662352923
49	20.659864853161814	19.19698342145731	34.90669157076016	25.236460154620715
50	20.815749498524244	18.561507727524877	32.53893020066052	28.083812573290363
51	20.293325281633933	19.19674936042824	29.451899288220414	31.05802606971742
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	171.0
1	366.0
2	561.0
3	1432.0
4	2303.0
5	1529.5
6	756.0
7	745.0
8	734.0
9	748.5
10	763.0
11	795.5
12	828.0
13	866.0
14	904.0
15	843.0
16	782.0
17	762.0
18	742.0
19	844.0
20	946.0
21	1027.0
22	1108.0
23	1250.5
24	1393.0
25	1908.5
26	2747.5
27	3071.0
28	3649.5
29	4228.0
30	4913.0
31	5598.0
32	6132.5
33	6667.0
34	7164.5
35	7662.0
36	8568.0
37	9474.0
38	10188.5
39	10903.0
40	12347.0
41	13791.0
42	16466.5
43	19142.0
44	23990.5
45	28839.0
46	45063.0
47	61287.0
48	55316.0
49	49345.0
50	47530.5
51	45716.0
52	38073.5
53	30431.0
54	26635.5
55	22840.0
56	20901.0
57	18962.0
58	17727.5
59	16493.0
60	15820.0
61	15147.0
62	13880.5
63	12614.0
64	11375.5
65	10137.0
66	8320.5
67	6504.0
68	5523.5
69	4543.0
70	3960.0
71	3377.0
72	2842.0
73	2307.0
74	1982.5
75	1329.5
76	1001.0
77	800.0
78	599.0
79	407.0
80	215.0
81	186.0
82	157.0
83	121.5
84	86.0
85	50.0
86	14.0
87	10.5
88	7.0
89	7.5
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	427239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.49841387983904
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.48022424525247	27.178934468520215
2	10.428612104642635	7.821128257843777
3	4.0251271055539695	4.52807646369064
4	2.2601009517945885	3.390008036024466
5	1.5289932166190388	2.8667410228123558
6	1.1789167228743709	2.6524504322524005
7	1.0141411736579242	2.6620079823687393
8	0.8671860463025179	2.6014481020058464
9	0.7560541322000716	2.5515747688320745
>10	5.344514804516952	31.339640126846717
>50	0.06904997094270217	1.8376715530330898
>100	0.03766362051420118	2.9913065320794807
>500	0.0018831810257100591	0.4218147077027407
>1k	0.005649543077130177	3.096760209005166
>5k	0.0018831810257100591	4.0604373369822975
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	6322	1.4797338257977384	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	5721	1.3390631473250334	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	5207	1.2187557783816552	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	2448	0.5729813991700197	TruSeq Adapter, Index 20 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGCT	1642	0.38432820973740695	TruSeq Adapter, Index 23 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCT	1521	0.35600682521960775	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCGCTTTT	1392	0.3258129524692268	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	1363	0.31902518262611795	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTC	1325	0.31013086352135455	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTA	1209	0.282979784148919	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1207	0.28251166209077355	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCT	1049	0.2455300194972837	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGC	619	0.14488377699601393	No Hit
TCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	594	0.1390322512691959	TruSeq Adapter, Index 20 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCG	579	0.13552133583310513	No Hit
GAATATGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTC	489	0.1144558432165603	No Hit
ACTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCCGTCTTCTGC	473	0.11071086675139676	TruSeq Adapter, Index 23 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCCGCTTTTCGTATGCC	471	0.11024274469325132	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4014146648597155	0.0
2	0.0	0.0	0.0	1.599807133712044	0.0
3	0.0	0.0	0.0	2.2727325922961152	0.0
4	0.0	0.0	0.0	3.27030069820405	0.0
5	0.0	0.0	0.0	6.164933444746383	0.0
6	0.0	0.0	0.0	7.519912742048362	0.0
7	0.0	0.0	0.0	9.13821069705715	0.0
8	0.0	0.0	0.0	11.500354602459046	0.0
9	0.0	0.0	0.0	12.348825832847657	0.0
10	0.0	0.0	0.0	15.368915290972968	0.0
11	0.0	0.0	0.0	17.335261996212893	0.0
12	0.0	0.0	0.0	20.556175817282597	0.0
13	0.0	0.0	0.0	21.31242700221656	0.0
14	0.0	0.0	0.0	21.664688850971004	0.0
15	0.0	0.0	0.0	22.43966491823078	0.0
16	0.0	0.0	0.0	23.445425160156258	0.0
17	0.0	0.0	0.0	24.55861941442612	0.0
18	0.0	0.0	0.0	25.798206624395245	0.0
19	0.0	0.0	0.0	26.907889963229014	0.0
20	0.0	0.0	0.0	27.688717556215607	0.0
21	2.3406102907272042E-4	0.0	0.0	28.482652566830275	0.0
22	2.3406102907272042E-4	0.0	0.0	29.329017247957232	0.0
23	2.3406102907272042E-4	0.0	0.0	30.093460568908736	0.0
24	2.3406102907272042E-4	0.0	0.0	30.710211380515357	0.0
25	2.3406102907272042E-4	0.0	0.0	31.223273156242758	0.0
26	2.3406102907272042E-4	0.0	0.0	31.72837685698169	0.0
27	2.3406102907272042E-4	0.0	0.0	32.26531285767451	0.0
28	2.3406102907272042E-4	0.0	0.0	32.780481182663564	0.0
29	2.3406102907272042E-4	0.0	0.0	33.31928967158897	0.0
30	2.3406102907272042E-4	0.0	0.0	33.92129463836401	0.0
31	2.3406102907272042E-4	0.0	0.0	34.44325073319617	0.0
32	2.3406102907272042E-4	0.0	0.0	34.93946011483034	0.0
33	2.3406102907272042E-4	0.0	0.0	35.40196470827804	0.0
34	2.3406102907272042E-4	0.0	0.0	35.8707889495107	0.0
35	2.3406102907272042E-4	0.0	0.0	36.3581040120401	0.0
36	2.3406102907272042E-4	0.0	0.0	36.84588719662765	0.0
37	2.3406102907272042E-4	0.0	0.0	37.30722148492998	0.0
38	2.3406102907272042E-4	0.0	0.0	37.79125969305237	0.0
39	2.3406102907272042E-4	0.0	0.0	38.26968043647701	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTACTAC	20	7.028303E-4	45.000004	31
GCGAAAG	20	7.028303E-4	45.000004	1
TTCCGTA	20	7.028303E-4	45.000004	24
CACGAAG	20	7.028303E-4	45.000004	1
AGTATGC	20	7.028303E-4	45.000004	42
ACTACCA	20	7.028303E-4	45.000004	18
GAACGTC	20	7.028303E-4	45.000004	12
AATTCGC	20	7.028303E-4	45.000004	13
ACGTCCC	20	7.028303E-4	45.000004	14
TTCGAAT	40	6.7975634E-9	45.000004	43
CAGGTAC	25	3.886676E-5	45.0	5
GTGCAAG	45	3.8380676E-10	45.0	1
CGGCGAA	25	3.886676E-5	45.0	31
CTCACGA	25	3.886676E-5	45.0	24
TACGGGT	25	3.886676E-5	45.0	4
AGTTGCG	25	3.886676E-5	45.0	1
CGGTCTA	25	3.886676E-5	45.0	31
ATACGAG	25	3.886676E-5	45.0	1
TGCGTAG	25	3.886676E-5	45.0	1
CTTACGG	50	2.1827873E-11	45.0	2
>>END_MODULE