FastQCFastQC Report
Sat 18 Jun 2016
SRR3550063_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550063_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences732424
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT36890.5036700053520912TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC36870.5033969394776796TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC23640.32276386355444386TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC18870.25763765250729087No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.2449400893471541No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC15390.21012419035968238No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG15260.20834926217600733No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC13870.1893711839044051TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC9960.13598680545694844TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC8550.11673566131093466No Hit
AGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG7730.10553996046006138TruSeq Adapter, Index 15 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAA207.0316077E-445.00000432
TTCACGA207.0316077E-445.00000419
ACTTCGC302.1644191E-645.00000439
GTTAACG302.1644191E-645.0000041
GGGCGTA207.0316077E-445.0000047
TGCTACG406.8084773E-945.0000041
TAGGCGT207.0316077E-445.0000044
ACGTAAG207.0316077E-445.0000041
ATACGGT302.1644191E-645.00000418
ACGAGTA207.0316077E-445.00000411
CGTTTCG207.0316077E-445.00000442
TCGAAAT207.0316077E-445.00000417
ACGCGAG253.889415E-545.01
TTTAGCG351.2111195E-745.01
TCGTTAG253.889415E-545.01
ACGTTAC253.889415E-545.017
CTACGAA253.889415E-545.011
CGAAGTC253.889415E-545.034
AAGCACG603.6379788E-1241.2500041
CGTTTTT8900.040.449441
Produced by FastQC (version 0.11.5)