##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550063_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	732424
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88416818673337	33.0	31.0	34.0	30.0	34.0
2	31.969744301115202	33.0	31.0	34.0	30.0	34.0
3	31.959793780651644	33.0	31.0	34.0	30.0	34.0
4	35.67998454447151	37.0	35.0	37.0	33.0	37.0
5	31.351093082695268	37.0	35.0	37.0	0.0	37.0
6	33.135623627843984	37.0	35.0	37.0	17.0	37.0
7	35.19949237053947	37.0	35.0	37.0	32.0	37.0
8	35.43997056349874	37.0	35.0	37.0	33.0	37.0
9	37.4700378469302	39.0	37.0	39.0	35.0	39.0
10	37.025279073323645	39.0	37.0	39.0	33.0	39.0
11	37.059488766069926	39.0	37.0	39.0	33.0	39.0
12	37.06546344740205	39.0	37.0	39.0	33.0	39.0
13	36.9463930728649	39.0	37.0	39.0	33.0	39.0
14	37.964321212849384	40.0	37.0	41.0	33.0	41.0
15	38.16258342162463	40.0	37.0	41.0	33.0	41.0
16	38.06201872139635	40.0	37.0	41.0	33.0	41.0
17	38.09374624534423	40.0	37.0	41.0	33.0	41.0
18	38.11769412253012	40.0	37.0	41.0	34.0	41.0
19	38.08074967505161	40.0	37.0	41.0	34.0	41.0
20	38.15067501884155	40.0	37.0	41.0	34.0	41.0
21	38.05030419538409	40.0	37.0	41.0	34.0	41.0
22	38.05597850425437	40.0	37.0	41.0	34.0	41.0
23	37.80069877557262	40.0	36.0	41.0	33.0	41.0
24	37.59234132142038	39.0	36.0	41.0	33.0	41.0
25	37.45804479372604	39.0	36.0	41.0	32.0	41.0
26	37.75856607648029	39.0	36.0	41.0	33.0	41.0
27	37.870008355815756	40.0	36.0	41.0	34.0	41.0
28	37.786994964665276	40.0	36.0	41.0	33.0	41.0
29	37.81778587266392	40.0	36.0	41.0	33.0	41.0
30	37.65288821775365	40.0	36.0	41.0	33.0	41.0
31	37.59176788308412	40.0	36.0	41.0	33.0	41.0
32	37.477477526678534	40.0	36.0	41.0	33.0	41.0
33	37.43931520540015	40.0	36.0	41.0	33.0	41.0
34	37.375857426845656	40.0	36.0	41.0	33.0	41.0
35	37.325878179852104	40.0	36.0	41.0	33.0	41.0
36	37.24962726508143	39.0	36.0	41.0	32.0	41.0
37	37.219628248118575	39.0	36.0	41.0	32.0	41.0
38	37.01652321606064	39.0	35.0	41.0	31.0	41.0
39	37.0325180496543	39.0	35.0	41.0	32.0	41.0
40	37.01681403121689	39.0	35.0	41.0	32.0	41.0
41	36.95078124146669	39.0	35.0	41.0	31.0	41.0
42	37.012398556027655	39.0	35.0	41.0	32.0	41.0
43	36.9162889255404	39.0	35.0	41.0	32.0	41.0
44	36.833439373914565	39.0	35.0	41.0	31.0	41.0
45	36.76141551887977	39.0	35.0	41.0	31.0	41.0
46	36.68548818717027	39.0	35.0	40.0	31.0	41.0
47	36.625312660426204	39.0	35.0	40.0	31.0	41.0
48	36.589938341725556	39.0	35.0	40.0	31.0	41.0
49	36.57977073389184	39.0	35.0	40.0	31.0	41.0
50	36.466595851583236	39.0	35.0	40.0	31.0	41.0
51	35.38481671818509	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	11.0
10	6.0
11	12.0
12	14.0
13	8.0
14	19.0
15	26.0
16	46.0
17	69.0
18	126.0
19	308.0
20	522.0
21	858.0
22	1400.0
23	1969.0
24	2787.0
25	3609.0
26	4575.0
27	5472.0
28	6610.0
29	8141.0
30	10708.0
31	14251.0
32	19520.0
33	28389.0
34	45576.0
35	60333.0
36	69136.0
37	107122.0
38	162816.0
39	177915.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.66125086015751	21.220631765207038	26.1685854095442	10.949531965091259
2	31.074623442159186	24.580707349841074	26.656963725929245	17.687705482070495
3	30.918566294932994	24.07690081155178	28.414415693641935	16.590117199873298
4	27.116806658438282	25.849234869419902	27.96576846198377	19.06819001015805
5	22.059353598462092	37.895399386147915	22.632109270040303	17.413137745349687
6	25.108407152141382	34.87706574333993	24.821687984009262	15.192839120509433
7	79.49930641267899	5.263754328094109	9.841157580854805	5.39578167837209
8	79.28139984489859	4.867672277260167	9.209692746278114	6.641235131563137
9	72.57094251417212	7.3520256026563855	12.160715651043658	7.916316232127839
10	34.17596911078829	33.260788832698005	17.90356405579282	14.659678000720893
11	26.65245813900145	24.419188885126648	29.217229364411875	19.711123611460028
12	24.914257315434778	20.95999038808122	32.93761537033194	21.188136926152065
13	24.734033838323157	23.265895164549498	34.328066802835515	17.672004194291834
14	20.336444463862463	28.29713390058218	30.652054001507324	20.71436763404804
15	17.62435419920702	25.310066300394308	37.29520059419134	19.770378906207334
16	19.87387087260931	25.99136565705111	31.68506220440619	22.449701265933395
17	19.650912586152284	25.131481218529157	29.66778805719092	25.549818138127645
18	20.554214498705665	23.71973064782148	32.733498629209315	22.99255622426354
19	20.304086157744695	26.481655434557034	30.46500387753542	22.749254530162858
20	21.95968455430188	26.292420783589833	30.351135407905804	21.396759254202482
21	20.928178213712275	27.82199927910609	30.843609712407023	20.40621279477461
22	19.20090002512206	24.804075235109718	29.008880102235864	26.986144637532362
23	18.346340371151136	27.40775834762378	29.410150404683627	24.835750876541457
24	19.507416469149018	25.26842375454655	30.47387851845379	24.75028125785064
25	18.735868840999203	27.377311502626895	28.29959149345188	25.58722816292202
26	19.356684106473846	27.180567540113376	29.571395803523643	23.891352549889135
27	22.04488110711828	26.067687568949133	29.37260384695204	22.51482747698055
28	19.0717398665254	25.334505696154142	32.23856673183839	23.35518770548207
29	22.018803316111978	24.913301584874333	29.342293534892356	23.72560156412133
30	24.641737572772055	26.306620209059233	27.97764682752067	21.07399539064804
31	23.639585813681695	27.15134949155134	26.904771006957716	22.30429368780925
32	23.813938374493464	29.69864450099942	24.742498880429913	21.7449182440772
33	23.534591984970454	27.714820923399564	25.64170480486713	23.108882286762856
34	22.91377671949581	25.65808875733182	28.319661835221126	23.10847268795124
35	22.468133212456173	27.76137865498673	26.24053826745164	23.529949865105458
36	23.930127904055574	26.87336843140039	27.445987570041396	21.750516094502636
37	23.105059364521097	27.390145598724235	29.272934802791827	20.23186023396284
38	19.227523947877188	31.276965255098137	26.85575568250085	22.639755114523826
39	22.461716164407502	27.466740576496672	26.7009273317095	23.370615927386325
40	24.051778751105918	25.540397365460443	28.218763994626062	22.189059888807574
41	21.81250204799406	26.144009480847156	26.02700075366181	26.016487717496968
42	23.313545159634312	24.673140148329384	27.735437396917632	24.277877295118675
43	22.728091924895963	23.815030637991107	28.681883717628043	24.77499371948489
44	21.684980284643867	24.785097156838116	28.748784856858865	24.781137701659148
45	22.23780214739004	24.389561237752996	28.26832545083176	25.104311164025212
46	24.839300732908807	24.992354155516477	27.68887966533046	22.47946544624425
47	20.854313894683955	24.665903902657476	30.709261302196545	23.770520900462028
48	20.21001496400992	24.290711391216018	29.815789761121973	25.68348388365209
49	22.3528994134545	22.257189824473254	31.276282590412112	24.11362817166013
50	20.996035083503546	21.791339442727164	31.047180321780825	26.165445151988465
51	20.713821502299215	22.126391270630126	27.540877961399406	29.61890926567125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	240.0
1	455.0
2	670.0
3	1997.5
4	3325.0
5	2200.5
6	1076.0
7	1156.0
8	1236.0
9	1242.5
10	1249.0
11	1269.0
12	1289.0
13	1307.0
14	1325.0
15	1373.5
16	1422.0
17	1397.0
18	1372.0
19	1640.5
20	1909.0
21	1993.5
22	2078.0
23	2523.0
24	2968.0
25	3464.0
26	4594.0
27	5228.0
28	6291.5
29	7355.0
30	9310.5
31	11266.0
32	12690.5
33	14115.0
34	15993.5
35	17872.0
36	19001.0
37	20130.0
38	22463.0
39	24796.0
40	27888.0
41	30980.0
42	36971.0
43	42962.0
44	50531.0
45	58100.0
46	70765.0
47	83430.0
48	91436.0
49	99442.0
50	93987.0
51	88532.0
52	73653.0
53	58774.0
54	49544.0
55	40314.0
56	34338.5
57	28363.0
58	24986.5
59	21610.0
60	19409.5
61	17209.0
62	15090.0
63	12971.0
64	11283.5
65	9596.0
66	7642.5
67	5689.0
68	4484.0
69	3279.0
70	2788.0
71	2297.0
72	1982.5
73	1668.0
74	1320.0
75	819.5
76	667.0
77	497.0
78	327.0
79	245.0
80	163.0
81	147.5
82	132.0
83	86.5
84	41.0
85	31.0
86	21.0
87	11.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	732424.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.035266914476445
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.16970837887247	24.93449729279248
2	12.21113430626864	8.55640699497284
3	4.728889808263678	4.970337499249959
4	2.3586895521243414	3.305492721082528
5	1.4598854683639244	2.557373852434777
6	1.0188290066950125	2.1416967713862403
7	0.7051076635154047	1.7292544636293528
8	0.5539109147576915	1.5525133396302027
9	0.4332172112395178	1.3660092564949466
>10	5.154110707038483	38.778317031107015
>50	0.14456188837174797	3.268860125593933
>100	0.05560072582355159	3.558970915046558
>500	0.003177184332774377	0.7933869793414449
>1k	0.003177184332774377	2.4868827572377485
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGCT	3689	0.5036700053520912	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	3687	0.5033969394776796	TruSeq Adapter, Index 14 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	2364	0.32276386355444386	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGC	1887	0.25763765250729087	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1794	0.2449400893471541	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTC	1539	0.21012419035968238	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCG	1526	0.20834926217600733	No Hit
TCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	1387	0.1893711839044051	TruSeq Adapter, Index 14 (95% over 21bp)
ACTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTGC	996	0.13598680545694844	TruSeq Adapter, Index 15 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCC	855	0.11673566131093466	No Hit
AGCTGTCTCTTATACACATCTGACGCGGTAAGACTCGTATGCCGTCTTCTG	773	0.10553996046006138	TruSeq Adapter, Index 15 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5346629820978013	0.0
2	0.0	0.0	0.0	1.7668727403798892	0.0
3	0.0	0.0	0.0	2.53500704509956	0.0
4	0.0	0.0	0.0	3.4703941978963004	0.0
5	0.0	0.0	0.0	5.579145413039441	0.0
6	0.0	0.0	0.0	6.9350540124299584	0.0
7	0.0	0.0	0.0	8.28754928839033	0.0
8	0.0	0.0	0.0	10.769171955042435	0.0
9	0.0	0.0	0.0	11.723264120236365	0.0
10	0.0	0.0	0.0	13.81904470634496	0.0
11	0.0	0.0	0.0	16.225983856345504	0.0
12	1.365329372057715E-4	0.0	0.0	18.418429761995785	0.0
13	1.365329372057715E-4	0.0	0.0	19.273808613589942	0.0
14	1.365329372057715E-4	0.0	0.0	19.58537677629351	0.0
15	1.365329372057715E-4	0.0	0.0	20.18857929286861	0.0
16	1.365329372057715E-4	0.0	0.0	21.273060413094054	0.0
17	1.365329372057715E-4	0.0	0.0	22.716486625233472	0.0
18	1.365329372057715E-4	0.0	0.0	24.37263115353948	0.0
19	1.365329372057715E-4	0.0	0.0	25.373963715006607	0.0
20	1.365329372057715E-4	0.0	0.0	26.291055454217776	0.0
21	1.365329372057715E-4	0.0	0.0	27.308362369337978	0.0
22	1.365329372057715E-4	0.0	0.0	28.350381746092427	0.0
23	1.365329372057715E-4	0.0	0.0	29.392810721658492	0.0
24	1.365329372057715E-4	0.0	0.0	30.18388255982873	0.0
25	1.365329372057715E-4	0.0	0.0	30.90436686946359	0.0
26	1.365329372057715E-4	0.0	0.0	31.556448177558355	0.0
27	1.365329372057715E-4	0.0	0.0	32.23392461197339	0.0
28	1.365329372057715E-4	0.0	0.0	32.8892827105611	0.0
29	1.365329372057715E-4	0.0	0.0	33.58450842681288	0.0
30	1.365329372057715E-4	0.0	0.0	34.39838126549649	0.0
31	1.365329372057715E-4	0.0	0.0	35.10384695203871	0.0
32	1.365329372057715E-4	0.0	0.0	35.7658951645495	0.0
33	1.365329372057715E-4	0.0	0.0	36.41838607145588	0.0
34	1.365329372057715E-4	0.0	0.0	37.03182855832141	0.0
35	1.365329372057715E-4	0.0	0.0	37.70862232805042	0.0
36	1.365329372057715E-4	0.0	0.0	38.33817570150623	0.0
37	1.365329372057715E-4	0.0	0.0	38.95202778718338	0.0
38	1.365329372057715E-4	0.0	0.0	39.552226579139955	0.0
39	1.365329372057715E-4	0.0	0.0	40.17481677279827	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCAA	20	7.0316077E-4	45.000004	32
TTCACGA	20	7.0316077E-4	45.000004	19
ACTTCGC	30	2.1644191E-6	45.000004	39
GTTAACG	30	2.1644191E-6	45.000004	1
GGGCGTA	20	7.0316077E-4	45.000004	7
TGCTACG	40	6.8084773E-9	45.000004	1
TAGGCGT	20	7.0316077E-4	45.000004	4
ACGTAAG	20	7.0316077E-4	45.000004	1
ATACGGT	30	2.1644191E-6	45.000004	18
ACGAGTA	20	7.0316077E-4	45.000004	11
CGTTTCG	20	7.0316077E-4	45.000004	42
TCGAAAT	20	7.0316077E-4	45.000004	17
ACGCGAG	25	3.889415E-5	45.0	1
TTTAGCG	35	1.2111195E-7	45.0	1
TCGTTAG	25	3.889415E-5	45.0	1
ACGTTAC	25	3.889415E-5	45.0	17
CTACGAA	25	3.889415E-5	45.0	11
CGAAGTC	25	3.889415E-5	45.0	34
AAGCACG	60	3.6379788E-12	41.250004	1
CGTTTTT	890	0.0	40.44944	1
>>END_MODULE