FastQCFastQC Report
Sat 18 Jun 2016
SRR3550060_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550060_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441508
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC27530.6235447602308453No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC27100.613805412359459No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG25540.5784719642679181No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC15000.3397446931878924No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12710.28787700336120753No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT12240.27723166964132023No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC8440.1911630140337208No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGCTAAGT6470.14654321099504428No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT6370.14427824637379164No Hit
GAACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCT6340.14359875698741587No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC5640.12774400463864755No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTA5480.12412006124464336No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT4570.10350888319124456No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCC4440.10056442918361616No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTCG453.8380676E-1045.00000415
GCGTAAG302.1624528E-645.0000041
CGACGTT207.028562E-445.027
ACGATAG253.8868897E-545.01
GCGATAT207.028562E-445.09
CGTAAGG406.7993824E-945.02
AGCTCGA253.8868897E-545.025
GCGCTAA207.028562E-445.010
TACGTTC207.028562E-445.023
AGTAACG207.028562E-445.01
CGTTCGG1350.041.66666845
GCGATGA1250.041.49
CACGACC603.6379788E-1241.25000427
TAGGACG451.9237632E-840.0000041
CATACGA850.039.70588318
CGCATGG403.451769E-739.3752
AACGGTC403.451769E-739.37520
ATGGGCG1550.039.1935465
TAACGGG1550.039.1935463
GGCGATA1150.039.1304368
Produced by FastQC (version 0.11.5)