##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550060_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	441508
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97168114734048	33.0	31.0	34.0	30.0	34.0
2	32.067162995914	33.0	31.0	34.0	30.0	34.0
3	32.04975447783506	33.0	31.0	34.0	30.0	34.0
4	35.74824918234777	37.0	35.0	37.0	35.0	37.0
5	31.6597003904799	37.0	35.0	37.0	0.0	37.0
6	33.30922202995189	37.0	35.0	37.0	17.0	37.0
7	35.21739356931245	37.0	35.0	37.0	32.0	37.0
8	35.361554037525934	37.0	35.0	37.0	33.0	37.0
9	37.40387943140328	39.0	37.0	39.0	34.0	39.0
10	37.043292533770625	39.0	37.0	39.0	33.0	39.0
11	37.06627965971172	39.0	37.0	39.0	33.0	39.0
12	37.06549145202352	39.0	37.0	39.0	33.0	39.0
13	36.95933256022541	39.0	37.0	39.0	33.0	39.0
14	38.03052039827138	40.0	37.0	41.0	33.0	41.0
15	38.2052284443317	40.0	37.0	41.0	33.0	41.0
16	38.141573878616015	40.0	37.0	41.0	33.0	41.0
17	38.11123920744358	40.0	37.0	41.0	33.0	41.0
18	38.11543618688676	40.0	37.0	41.0	34.0	41.0
19	38.07689781385615	40.0	37.0	41.0	34.0	41.0
20	38.06972240593602	40.0	37.0	41.0	34.0	41.0
21	37.985366063582084	40.0	37.0	41.0	33.0	41.0
22	37.97333004158475	40.0	37.0	41.0	33.0	41.0
23	37.73926633265988	40.0	36.0	41.0	33.0	41.0
24	37.50385496978537	39.0	36.0	41.0	32.0	41.0
25	37.34010029263343	39.0	35.0	41.0	32.0	41.0
26	37.586023356315174	39.0	36.0	41.0	33.0	41.0
27	37.683124654592895	40.0	36.0	41.0	33.0	41.0
28	37.60335713056162	40.0	36.0	41.0	33.0	41.0
29	37.60993911775098	40.0	36.0	41.0	33.0	41.0
30	37.46665066091668	40.0	36.0	41.0	33.0	41.0
31	37.449831033639256	40.0	36.0	41.0	33.0	41.0
32	37.33979452240956	40.0	36.0	41.0	33.0	41.0
33	37.28711824021309	40.0	35.0	41.0	33.0	41.0
34	37.141786785290414	39.0	35.0	41.0	32.0	41.0
35	37.08160894026835	39.0	35.0	41.0	32.0	41.0
36	36.98063455248829	39.0	35.0	41.0	32.0	41.0
37	36.92147367658117	39.0	35.0	41.0	31.0	41.0
38	36.80600125026047	39.0	35.0	41.0	31.0	41.0
39	36.76361470233835	39.0	35.0	41.0	31.0	41.0
40	36.623236725042354	39.0	35.0	41.0	31.0	41.0
41	36.4912934759959	39.0	35.0	40.0	31.0	41.0
42	36.50982541652699	39.0	35.0	40.0	31.0	41.0
43	36.43353914311859	39.0	35.0	40.0	31.0	41.0
44	36.4008330539877	39.0	35.0	40.0	31.0	41.0
45	36.37477916594943	39.0	35.0	40.0	31.0	41.0
46	36.28590874910534	38.0	35.0	40.0	31.0	41.0
47	36.188046422714876	38.0	35.0	40.0	30.0	41.0
48	36.139825325928406	38.0	35.0	40.0	30.0	41.0
49	36.091506835663225	38.0	35.0	40.0	30.0	41.0
50	35.95550024008625	38.0	35.0	40.0	30.0	41.0
51	34.92638185491543	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	9.0
10	8.0
11	13.0
12	8.0
13	11.0
14	16.0
15	26.0
16	35.0
17	56.0
18	131.0
19	232.0
20	543.0
21	893.0
22	1236.0
23	1549.0
24	2050.0
25	2689.0
26	3162.0
27	3797.0
28	4478.0
29	5479.0
30	6606.0
31	8807.0
32	12114.0
33	17310.0
34	28457.0
35	36452.0
36	42201.0
37	63151.0
38	93605.0
39	106340.0
40	39.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.46625655707258	20.22522808193736	24.36852786359477	11.939987497395292
2	31.717205577248887	27.54536724136369	23.664350362847333	17.073076818540095
3	30.943267166166866	26.6892106145302	26.054794024117346	16.312728195185592
4	27.758273915761432	25.23170588075414	28.53651575962384	18.473504443860588
5	24.588682424780526	36.42516103898457	21.129175462279278	17.856981073955623
6	26.317982913106896	35.976924540438674	23.497875463185263	14.207217083269159
7	78.21534377633022	5.621868686411118	11.373972838544262	4.788814698714406
8	77.46450800438497	7.553204018953225	9.239470179475797	5.742817797186008
9	71.24083821810703	7.239279922447611	11.649845529412831	9.870036330032525
10	39.71819310182375	24.413600659557698	18.595359540483976	17.272846698134575
11	30.358453300959436	24.05936019279379	24.884939797240367	20.697246709006407
12	26.64572329380215	20.233381954573872	30.96976725223552	22.15112749938846
13	25.185727098942714	21.705382461925947	32.86916658361797	20.23972385551338
14	20.088424218813703	28.125424680866484	29.394257861692203	22.391893238627613
15	18.7874285403662	23.997979651557845	35.501961459362	21.712630348713954
16	20.6057421383078	24.108283428612847	30.746894733504266	24.53907969957509
17	20.795772670030892	24.09424064796108	29.34193717894126	25.768049503066763
18	22.07411870226587	24.05777471755891	29.63230564338585	24.235800936789367
19	23.467751433722604	25.554010346358393	26.56169310635368	24.416545113565327
20	25.1963724326626	24.272719860115785	27.98771483189433	22.543192875327286
21	23.773748154053834	27.014912527066326	27.165759170841753	22.045580148038088
22	21.69836107160006	23.84849198655517	27.424191634126675	27.028955307718093
23	22.59234260760847	26.00519129891191	27.372550440762115	24.029915652717506
24	23.11419045634507	23.85891082381293	27.785453491216465	25.24144522862553
25	21.769933953631647	26.619223207733494	26.187973943847	25.422868894787864
26	21.145936200476548	26.09714886253477	27.592931498409996	25.16398343857869
27	24.590947389401776	25.36986872265055	26.096922366072643	23.94226152187503
28	21.983520117415768	26.13180282123993	27.709124183480256	24.17555287786405
29	23.23763102820334	25.061380541235945	26.484004819844714	25.216983610716
30	25.546082970184003	25.483569946637434	25.970537340206747	22.999809742971813
31	25.5639761906919	26.196127816483504	23.5735252815351	24.66637071128949
32	25.04326082426592	27.580021200068856	23.42539659530518	23.95132138036004
33	25.459108328727904	25.06160703769807	25.026047093144406	24.45323754042962
34	23.772842168205333	27.076746061226526	25.156962048252808	23.993449722315336
35	23.843509064388414	25.955815070168605	25.013589787727515	25.187086077715463
36	26.057285485200723	27.80968861266387	24.81019596473903	21.322829937396378
37	25.308714677876733	27.022613406778582	25.691493698868424	21.977178216476258
38	24.144749359015012	25.905532855576794	25.556275310979643	24.39344247442855
39	24.843490944671444	25.098072968100237	25.80496842639318	24.25346766083514
40	22.778069706551186	26.04211022223833	29.16617592433206	22.013644146878423
41	22.5092184060085	27.53155095717405	25.537476104623245	24.421754532194207
42	24.084954293013944	25.946302218759342	26.474718464897578	23.494025023329137
43	26.084012067731503	23.738867698886544	26.608124881089356	23.568995352292596
44	24.082009839006314	23.831957744820027	26.355807822281818	25.730224593891844
45	22.91917700245522	23.10852804479194	26.792266504797198	27.180028447955646
46	24.524130933074826	25.49421528035732	26.431231144169526	23.550422642398324
47	22.065738333167236	25.13770984897216	29.408980131730345	23.38757168613026
48	23.04782699294237	23.978727452277194	27.20267809416817	25.770767460612266
49	23.135481123784846	22.64443679389728	29.808972883843555	24.41110919847432
50	21.905831830906802	22.96945921704703	29.176594761589826	25.948114190456344
51	22.112170107902916	22.863005879848156	26.568261503755313	28.456562508493615
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	153.0
1	339.0
2	525.0
3	1475.0
4	2425.0
5	1640.0
6	855.0
7	823.0
8	791.0
9	809.0
10	827.0
11	924.0
12	1021.0
13	944.0
14	867.0
15	913.5
16	960.0
17	930.0
18	900.0
19	1050.0
20	1200.0
21	1133.5
22	1067.0
23	1182.5
24	1298.0
25	1575.0
26	2359.0
27	2866.0
28	3648.5
29	4431.0
30	5345.5
31	6260.0
32	6747.0
33	7234.0
34	7987.0
35	8740.0
36	9656.0
37	10572.0
38	11418.5
39	12265.0
40	13871.5
41	15478.0
42	17956.0
43	20434.0
44	23936.0
45	27438.0
46	37012.5
47	46587.0
48	46088.0
49	45589.0
50	43756.0
51	41923.0
52	36475.0
53	31027.0
54	27705.5
55	24384.0
56	23308.5
57	22233.0
58	21435.5
59	20638.0
60	20031.5
61	19425.0
62	17800.0
63	16175.0
64	14917.0
65	13659.0
66	11506.0
67	9353.0
68	7973.5
69	6594.0
70	5714.0
71	4834.0
72	4179.0
73	3524.0
74	2883.0
75	1832.5
76	1423.0
77	1027.0
78	631.0
79	526.5
80	422.0
81	328.0
82	234.0
83	165.5
84	97.0
85	64.0
86	31.0
87	21.0
88	11.0
89	10.5
90	10.0
91	6.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	441508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.66047812996996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97492730268387	29.671954356269254
2	9.343467155901088	7.598196839012177
3	3.7783563902400306	4.608893321177611
4	2.367768380890795	3.8509837787217824
5	1.7486733003293495	3.5550946242251955
6	1.410315794444911	3.440646870982711
7	1.2717195391311706	3.619610715580891
8	1.09201937765463	3.5521624018103597
9	0.9462857973311197	3.462878967037486
>10	4.983521301105178	29.421961283403782
>50	0.04658591879183368	1.3058469517226932
>100	0.02954228996555299	2.242092784967351
>500	0.0034087257652561146	0.8948967081149308
>1k	0.0034087257652561146	2.7747803969738123
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGC	2753	0.6235447602308453	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTC	2710	0.613805412359459	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCG	2554	0.5784719642679181	No Hit
GCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	1500	0.3397446931878924	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1271	0.28787700336120753	No Hit
CTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGCT	1224	0.27723166964132023	No Hit
CCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCTGC	844	0.1911630140337208	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGGCTAAGT	647	0.14654321099504428	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCT	637	0.14427824637379164	No Hit
GAACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTTCT	634	0.14359875698741587	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTC	564	0.12774400463864755	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTA	548	0.12412006124464336	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCCGTCTT	457	0.10350888319124456	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGCTAAGTCGTATGCC	444	0.10056442918361616	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.29059496090671066	0.0
2	0.0	0.0	0.0	1.0353153283745709	0.0
3	0.0	0.0	0.0	1.5064279695951148	0.0
4	0.0	0.0	0.0	2.1677976390008786	0.0
5	0.0	0.0	0.0	3.953269249934316	0.0
6	0.0	0.0	0.0	5.031165913188436	0.0
7	0.0	0.0	0.0	6.124237839404948	0.0
8	0.0	0.0	0.0	7.88751279705011	0.0
9	0.0	0.0	0.0	8.59689971642643	0.0
10	0.0	0.0	0.0	10.474555387444848	0.0
11	0.0	0.0	0.0	12.011107386502623	0.0
12	0.0	0.0	0.0	13.907335767415313	0.0
13	0.0	0.0	0.0	14.467461518251085	0.0
14	0.0	0.0	0.0	14.73155639308914	0.0
15	0.0	0.0	0.0	15.266314540166883	0.0
16	0.0	0.0	0.0	16.02303922012738	0.0
17	0.0	0.0	0.0	16.919059224294916	0.0
18	0.0	0.0	0.0	17.93036592768421	0.0
19	0.0	0.0	0.0	18.67553928807632	0.0
20	0.0	0.0	0.0	19.277566884405264	0.0
21	0.0	0.0	0.0	19.979705916993577	0.0
22	0.0	0.0	0.0	20.68161845311976	0.0
23	0.0	0.0	0.0	21.352274477472662	0.0
24	0.0	0.0	0.0	21.88023773068665	0.0
25	0.0	0.0	0.0	22.342517009884304	0.0
26	0.0	0.0	0.0	22.784185111028567	0.0
27	0.0	0.0	0.0	23.26911403643875	0.0
28	4.529929242505232E-4	0.0	0.0	23.716671045598268	0.0
29	4.529929242505232E-4	0.0	0.0	24.195258070068945	0.0
30	4.529929242505232E-4	0.0	0.0	24.763990686465476	0.0
31	6.794893863757848E-4	0.0	0.0	25.247334136640784	0.0
32	6.794893863757848E-4	0.0	0.0	25.724562182338712	0.0
33	6.794893863757848E-4	0.0	0.0	26.197486795256257	0.0
34	6.794893863757848E-4	0.0	0.0	26.644590811491526	0.0
35	6.794893863757848E-4	0.0	0.0	27.106190601302806	0.0
36	6.794893863757848E-4	0.0	0.0	27.55397410692445	0.0
37	6.794893863757848E-4	0.0	0.0	28.004475570091596	0.0
38	6.794893863757848E-4	0.0	0.0	28.451806082788988	0.0
39	6.794893863757848E-4	0.0	0.0	28.914085361986647	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATTCG	45	3.8380676E-10	45.000004	15
GCGTAAG	30	2.1624528E-6	45.000004	1
CGACGTT	20	7.028562E-4	45.0	27
ACGATAG	25	3.8868897E-5	45.0	1
GCGATAT	20	7.028562E-4	45.0	9
CGTAAGG	40	6.7993824E-9	45.0	2
AGCTCGA	25	3.8868897E-5	45.0	25
GCGCTAA	20	7.028562E-4	45.0	10
TACGTTC	20	7.028562E-4	45.0	23
AGTAACG	20	7.028562E-4	45.0	1
CGTTCGG	135	0.0	41.666668	45
GCGATGA	125	0.0	41.4	9
CACGACC	60	3.6379788E-12	41.250004	27
TAGGACG	45	1.9237632E-8	40.000004	1
CATACGA	85	0.0	39.705883	18
CGCATGG	40	3.451769E-7	39.375	2
AACGGTC	40	3.451769E-7	39.375	20
ATGGGCG	155	0.0	39.193546	5
TAACGGG	155	0.0	39.193546	3
GGCGATA	115	0.0	39.130436	8
>>END_MODULE