FastQCFastQC Report
Sat 18 Jun 2016
SRR3550057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559526
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT21700.38782826892762806No Hit
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC18740.33492634837344465No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15330.27398190611338885No Hit
CCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC12670.2264416666964538No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCC7900.1411909366142056No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGC6990.12492717049788571No Hit
TCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC6810.1217101618155367No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTC6330.11313147199593943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG253.8882295E-545.0000041
CAACGAC351.2104283E-745.012
CGACGCA207.030179E-445.045
GCAACGA351.2104283E-745.011
TATTGCG351.2104283E-745.01
TCCAGCG207.030179E-445.018
TCGTAAG207.030179E-445.01
TTACCGG351.2104283E-745.02
CGTGAAT302.163497E-644.99999627
TATTACG302.163497E-644.9999961
CGCAATC302.163497E-644.99999620
CGTTATT1000.042.7500041
CGTTAGG800.042.18752
AGTTACG556.002665E-1140.9090921
TAACGGG1350.040.03
TTACGGG2500.039.63
CTAACGG403.453988E-739.3752
ATTGCGG750.039.02
TGATATG700.038.5714261
AATCCGA356.2425424E-638.57142632