##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	559526
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.969761905612966	33.0	31.0	34.0	30.0	34.0
2	32.05669620357231	33.0	31.0	34.0	30.0	34.0
3	32.04179430446485	33.0	31.0	34.0	30.0	34.0
4	35.74897323806222	37.0	35.0	37.0	35.0	37.0
5	31.38867184009322	37.0	35.0	37.0	0.0	37.0
6	33.19825888341203	37.0	35.0	37.0	17.0	37.0
7	35.23188377305076	37.0	35.0	37.0	32.0	37.0
8	35.42687024374202	37.0	35.0	37.0	33.0	37.0
9	37.459799902059956	39.0	37.0	39.0	35.0	39.0
10	37.084164096038435	39.0	37.0	39.0	34.0	39.0
11	37.11433070134364	39.0	37.0	39.0	34.0	39.0
12	37.12332760229194	39.0	37.0	39.0	34.0	39.0
13	37.030926176799646	39.0	37.0	39.0	33.0	39.0
14	38.08801199586793	40.0	37.0	41.0	33.0	41.0
15	38.287997340606154	40.0	37.0	41.0	34.0	41.0
16	38.18027223042361	40.0	37.0	41.0	33.0	41.0
17	38.17622416116499	40.0	37.0	41.0	34.0	41.0
18	38.182397243381004	40.0	37.0	41.0	34.0	41.0
19	38.131237869196426	40.0	37.0	41.0	34.0	41.0
20	38.20171538051851	40.0	37.0	41.0	34.0	41.0
21	38.059446745995714	40.0	37.0	41.0	34.0	41.0
22	38.07243809939127	40.0	37.0	41.0	34.0	41.0
23	37.83350729009912	40.0	36.0	41.0	33.0	41.0
24	37.58262708077909	39.0	36.0	41.0	33.0	41.0
25	37.429404531692896	39.0	36.0	41.0	32.0	41.0
26	37.69690237808431	39.0	36.0	41.0	33.0	41.0
27	37.81776360705311	40.0	36.0	41.0	33.0	41.0
28	37.73072386269807	40.0	36.0	41.0	33.0	41.0
29	37.74729145741217	40.0	36.0	41.0	33.0	41.0
30	37.61539231420881	40.0	36.0	41.0	33.0	41.0
31	37.568075478172595	40.0	36.0	41.0	33.0	41.0
32	37.436212079510156	39.0	36.0	41.0	33.0	41.0
33	37.415138885413725	39.0	36.0	41.0	33.0	41.0
34	37.309790787202026	39.0	35.0	41.0	33.0	41.0
35	37.230480799819844	39.0	35.0	41.0	32.0	41.0
36	37.132710544282126	39.0	35.0	41.0	32.0	41.0
37	37.06321243338111	39.0	35.0	41.0	32.0	41.0
38	36.9020510217577	39.0	35.0	41.0	32.0	41.0
39	36.88716878214775	39.0	35.0	41.0	31.0	41.0
40	36.8152042979236	39.0	35.0	41.0	31.0	41.0
41	36.73172292261664	39.0	35.0	40.0	31.0	41.0
42	36.71718025614538	39.0	35.0	40.0	31.0	41.0
43	36.618375196148165	39.0	35.0	40.0	31.0	41.0
44	36.56089261267573	39.0	35.0	40.0	31.0	41.0
45	36.52036545218631	39.0	35.0	40.0	31.0	41.0
46	36.46218942462013	39.0	35.0	40.0	31.0	41.0
47	36.41068690284276	38.0	35.0	40.0	31.0	41.0
48	36.316342761551745	38.0	35.0	40.0	31.0	41.0
49	36.284133355733246	38.0	35.0	40.0	31.0	41.0
50	36.16323459499648	38.0	35.0	40.0	31.0	41.0
51	35.06210435261275	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	8.0
10	12.0
11	13.0
12	12.0
13	15.0
14	17.0
15	26.0
16	57.0
17	75.0
18	126.0
19	271.0
20	428.0
21	690.0
22	1035.0
23	1570.0
24	2091.0
25	2742.0
26	3542.0
27	4513.0
28	5213.0
29	6467.0
30	8136.0
31	10892.0
32	14971.0
33	21909.0
34	35591.0
35	46470.0
36	55526.0
37	85574.0
38	121944.0
39	129519.0
40	66.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.16819236282139	20.780088860928714	25.003485092739215	12.048233683510686
2	32.158648570397084	24.436040505713766	25.626154995478316	17.779155928410834
3	32.59044262465015	24.26446670932182	26.346050049506186	16.79904061652184
4	28.68070473936868	26.07939577428037	26.45596451281978	18.783934973531167
5	22.04330093686442	38.7813971111262	21.5348348423487	17.640467109660676
6	25.679235638737076	35.26252578075014	23.97958271822936	15.078655862283433
7	79.36628503411816	5.997219074716814	9.29947848714805	5.337017404016971
8	78.72895986960391	5.7135861425563785	9.352737853111384	6.204716134728323
9	71.90461211811426	7.852718193613881	12.382981309179556	7.859688379092303
10	38.221816323102054	25.97984722783213	18.59019956177193	17.208136887293886
11	29.149851838877904	24.088603568020076	25.621865650568516	21.1396789425335
12	24.943255541297454	21.33448669052019	31.399434521362725	22.322823246819627
13	23.57549068318541	23.129041367157203	33.159316993312196	20.13615095634519
14	19.719190886571848	26.797861046671645	30.618237579665646	22.864710487090857
15	18.96801935924336	24.333989841401472	34.631634633600584	22.066356165754584
16	21.18328728244979	24.541308178708405	30.96335112219986	23.31205341664194
17	21.021543234809464	24.519146563341113	28.90589534713311	25.553414854716312
18	22.31495944781833	23.362274496627503	30.49545508162266	23.827310973931507
19	22.588762631227148	26.197710204708986	27.75384879344302	23.459678370620846
20	23.984586953957457	25.506768228822253	27.704342604275762	22.804302212944528
21	22.603954061116017	27.02680483123215	28.429956784850035	21.9392843228018
22	21.24762745609677	24.354721675132165	27.30561225036906	27.092038618402004
23	21.636885506660995	25.880656126793035	27.69594263716074	24.78651572938523
24	21.663515189642663	24.55721449941558	28.805453187161987	24.97381712377977
25	21.422418261171064	26.49421117159882	26.43862841047601	25.64474215675411
26	20.865160868306386	26.445241150545286	27.733831850530628	24.9557661306177
27	23.48827400335284	25.173271662085405	27.372811987289243	23.96564234727251
28	20.350975647244272	26.405385987425074	28.980958883054587	24.26267948227607
29	21.492298838659867	26.450424108977955	26.7874951298063	25.26978192255588
30	24.142041656687983	26.003259902131447	26.17930176613777	23.675396675042805
31	24.132033185231787	27.284701693933794	24.677137434185365	23.906127686649057
32	23.354768143035354	29.312489500041107	24.312006948738755	23.020735408184784
33	24.329164328377946	26.207539953460607	24.20781161197156	25.25548410618988
34	22.415580330494027	25.995396103130147	27.353331212490573	24.235692353885252
35	23.272376976226305	27.545994288022364	25.271211704192474	23.910417031558858
36	24.987042603918315	27.23787634533516	24.93396196065956	22.841119090086966
37	24.44479791823794	27.27272727272727	26.859877825159153	21.42259698387564
38	22.228815104213208	28.794014934069196	25.569857343537205	23.40731261818039
39	25.174701443722007	24.970600115097422	25.396317597394933	24.458380843785633
40	24.883740880673997	24.762388164267612	27.63803648087846	22.71583447417993
41	22.976233454745625	25.775745899207543	25.646350661095283	25.60166998495155
42	24.669631080593216	25.627584777114915	26.812158863037645	22.890625279254227
43	24.77722214874733	23.505610105696608	28.092707041317116	23.624460704238945
44	22.437563223156744	24.51575083195419	26.988558172453114	26.058127772435956
45	23.12743286281603	23.607660770008902	27.4344713203676	25.830435046807477
46	24.81904326161787	24.027837848464593	26.927613730193055	24.22550515972448
47	22.18484931888777	24.661946004296496	29.32732348452083	23.825881192294908
48	22.084049713507508	24.34953871669949	28.690177042711152	24.87623452708185
49	23.920246780310478	22.821817037992872	29.093911632345947	24.164024549350703
50	21.997011756379507	22.490286421006353	29.387553035962583	26.125148786651557
51	21.741080843428186	22.686345227925063	26.97801353288319	28.59456039576356
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	258.0
1	514.0
2	770.0
3	1817.5
4	2865.0
5	1914.5
6	964.0
7	941.5
8	919.0
9	948.5
10	978.0
11	1013.5
12	1049.0
13	1101.0
14	1153.0
15	1162.0
16	1171.0
17	1207.0
18	1243.0
19	1296.5
20	1350.0
21	1406.5
22	1463.0
23	1590.5
24	1718.0
25	2339.0
26	3693.0
27	4426.0
28	5094.5
29	5763.0
30	6656.0
31	7549.0
32	8192.0
33	8835.0
34	10211.5
35	11588.0
36	12684.0
37	13780.0
38	15093.5
39	16407.0
40	19128.0
41	21849.0
42	25034.5
43	28220.0
44	32644.0
45	37068.0
46	45006.0
47	52944.0
48	58251.5
49	63559.0
50	61694.0
51	59829.0
52	50998.0
53	42167.0
54	37108.0
55	32049.0
56	29337.5
57	26626.0
58	24569.5
59	22513.0
60	21486.5
61	20460.0
62	18852.5
63	17245.0
64	15584.5
65	13924.0
66	11888.5
67	9853.0
68	8650.5
69	7448.0
70	6591.0
71	5734.0
72	4921.5
73	4109.0
74	3436.5
75	2311.0
76	1858.0
77	1447.5
78	1037.0
79	825.0
80	613.0
81	440.0
82	267.0
83	186.0
84	105.0
85	75.0
86	45.0
87	33.5
88	22.0
89	13.5
90	5.0
91	4.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	559526.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.526024805582544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.28354414005886	28.570811587153877
2	10.151946984240741	8.02532216648117
3	4.023178927886413	4.770608103028061
4	2.3093320187658284	3.651148586322566
5	1.6028941439238762	3.1678016846729067
6	1.2117167813153638	2.8736608533367027
7	1.0660872327884556	2.9496733285677945
8	0.9200754134045046	2.9093538890586452
9	0.8301477263879152	2.953119566295602
>10	5.498993051684213	33.76068497435884
>50	0.06042615470924626	1.6492665048095247
>100	0.03707968584176198	2.7757978550687334
>500	0.002746643395686073	0.7043987386599687
>1k	0.0018310955971240484	1.238352162185673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGCT	2170	0.38782826892762806	No Hit
GCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	1874	0.33492634837344465	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1533	0.27398190611338885	No Hit
CCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	1267	0.2264416666964538	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCC	790	0.1411909366142056	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGC	699	0.12492717049788571	No Hit
TCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTCTGC	681	0.1217101618155367	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTACGAGTCGTATGCCGTCTTC	633	0.11313147199593943	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.787227045749438E-4	0.0	0.0	0.40981116159034614	0.0
2	1.787227045749438E-4	0.0	0.0	1.2380121745906356	0.0
3	1.787227045749438E-4	0.0	0.0	1.8197545779820776	0.0
4	1.787227045749438E-4	0.0	0.0	2.5282113789171548	0.0
5	1.787227045749438E-4	0.0	0.0	4.097039279676012	0.0
6	1.787227045749438E-4	0.0	0.0	5.327902546083649	0.0
7	1.787227045749438E-4	0.0	0.0	6.515157472575001	0.0
8	1.787227045749438E-4	0.0	0.0	8.66680011295275	0.0
9	1.787227045749438E-4	0.0	0.0	9.536643516119002	0.0
10	1.787227045749438E-4	0.0	0.0	11.173207321911761	0.0
11	1.787227045749438E-4	0.0	0.0	13.120033742846624	0.0
12	1.787227045749438E-4	0.0	0.0	14.729431697543992	0.0
13	1.787227045749438E-4	0.0	0.0	15.384807855220311	0.0
14	1.787227045749438E-4	0.0	0.0	15.670049291721922	0.0
15	1.787227045749438E-4	0.0	0.0	16.149919753505646	0.0
16	1.787227045749438E-4	0.0	0.0	17.067660841497982	0.0
17	3.574454091498876E-4	0.0	0.0	18.14625236360777	0.0
18	3.574454091498876E-4	0.0	0.0	19.399098522678123	0.0
19	3.574454091498876E-4	0.0	0.0	20.132933947662842	0.0
20	3.574454091498876E-4	0.0	0.0	20.87248849919396	0.0
21	3.574454091498876E-4	0.0	0.0	21.7039065208766	0.0
22	3.574454091498876E-4	0.0	0.0	22.548192577288635	0.0
23	3.574454091498876E-4	0.0	0.0	23.364419169082403	0.0
24	3.574454091498876E-4	0.0	0.0	24.033735697715567	0.0
25	3.574454091498876E-4	0.0	0.0	24.606005797764535	0.0
26	3.574454091498876E-4	0.0	0.0	25.120369741531224	0.0
27	3.574454091498876E-4	0.0	0.0	25.65814635959723	0.0
28	3.574454091498876E-4	0.0	0.0	26.211114407552106	0.0
29	3.574454091498876E-4	0.0	0.0	26.773912204258604	0.0
30	3.574454091498876E-4	0.0	0.0	27.44430106911922	0.0
31	3.574454091498876E-4	0.0	0.0	28.016749891872763	0.0
32	3.574454091498876E-4	0.0	0.0	28.60653481697008	0.0
33	3.574454091498876E-4	0.0	0.0	29.1798772532465	0.0
34	3.574454091498876E-4	0.0	0.0	29.703356054946507	0.0
35	3.574454091498876E-4	0.0	0.0	30.275804877700054	0.0
36	3.574454091498876E-4	0.0	0.0	30.799998570218364	0.0
37	3.574454091498876E-4	0.0	0.0	31.310251891779828	0.0
38	3.574454091498876E-4	0.0	0.0	31.82693923070599	0.0
39	3.574454091498876E-4	0.0	0.0	32.36864774827264	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	25	3.8882295E-5	45.000004	1
CAACGAC	35	1.2104283E-7	45.0	12
CGACGCA	20	7.030179E-4	45.0	45
GCAACGA	35	1.2104283E-7	45.0	11
TATTGCG	35	1.2104283E-7	45.0	1
TCCAGCG	20	7.030179E-4	45.0	18
TCGTAAG	20	7.030179E-4	45.0	1
TTACCGG	35	1.2104283E-7	45.0	2
CGTGAAT	30	2.163497E-6	44.999996	27
TATTACG	30	2.163497E-6	44.999996	1
CGCAATC	30	2.163497E-6	44.999996	20
CGTTATT	100	0.0	42.750004	1
CGTTAGG	80	0.0	42.1875	2
AGTTACG	55	6.002665E-11	40.909092	1
TAACGGG	135	0.0	40.0	3
TTACGGG	250	0.0	39.6	3
CTAACGG	40	3.453988E-7	39.375	2
ATTGCGG	75	0.0	39.0	2
TGATATG	70	0.0	38.571426	1
AATCCGA	35	6.2425424E-6	38.571426	32
>>END_MODULE