SRR3550054_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767701
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	3577	0.4659366081325933	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	2819	0.367200251139441	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	2795	0.3640740340314784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2580	0.33606833910598005	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2366	0.30819290322664683	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	2104	0.27406503313138836	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1398	0.18210214653882176	TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	989	0.12882619665729236	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC	806	0.10498879120907749	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	30	2.1645556E-6	45.000004	1
ACGTTAG	20	7.0318213E-4	45.0	1
ATTACCG	20	7.0318213E-4	45.0	1
TAATCGT	80	0.0	42.1875	21
CGTTATT	230	0.0	42.065216	1
TACGGGC	205	0.0	41.707317	4
TTTAGCG	55	6.002665E-11	40.909092	1
TACGGGT	110	0.0	40.909092	4
TGTTACG	55	6.002665E-11	40.909092	1
ACGGGAC	215	0.0	40.81395	5
TAACGCG	45	1.9268555E-8	40.0	1
TTAATCG	85	0.0	39.705883	20
TCATGCG	40	3.4562254E-7	39.375	1
ACGGGTA	75	0.0	39.0	5
CGTAAGG	75	0.0	39.0	2
CGTTTTT	1365	0.0	38.736263	1
TTGACGG	70	0.0	38.571426	2
TACGTTG	35	6.24558E-6	38.571426	1
ACATACG	135	0.0	38.333336	17
CGTTAGG	100	0.0	38.250004	2