##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550054_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	767701
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.932461987153854	33.0	31.0	34.0	30.0	34.0
2	32.02277318904105	33.0	31.0	34.0	30.0	34.0
3	32.00288393528209	33.0	31.0	34.0	30.0	34.0
4	35.71757233610481	37.0	35.0	37.0	33.0	37.0
5	31.476581377385205	37.0	35.0	37.0	0.0	37.0
6	33.20241995255966	37.0	35.0	37.0	17.0	37.0
7	35.17884306520377	37.0	35.0	37.0	32.0	37.0
8	35.360986894637364	37.0	35.0	37.0	33.0	37.0
9	37.39353211732172	39.0	37.0	39.0	34.0	39.0
10	37.014607249436956	39.0	37.0	39.0	33.0	39.0
11	37.011160595075424	39.0	37.0	39.0	33.0	39.0
12	36.96474408656495	39.0	37.0	39.0	33.0	39.0
13	36.85337390468425	39.0	35.0	39.0	33.0	39.0
14	37.89026977951051	40.0	37.0	41.0	33.0	41.0
15	38.06289948821221	40.0	37.0	41.0	33.0	41.0
16	37.97629676136933	40.0	37.0	41.0	33.0	41.0
17	37.930000091181334	40.0	36.0	41.0	33.0	41.0
18	37.91606628101305	40.0	36.0	41.0	33.0	41.0
19	37.86834848463138	40.0	36.0	41.0	33.0	41.0
20	37.885958205082446	40.0	36.0	41.0	33.0	41.0
21	37.78268883328275	40.0	36.0	41.0	33.0	41.0
22	37.7680542294461	40.0	36.0	41.0	33.0	41.0
23	37.53687828985503	39.0	35.0	41.0	33.0	41.0
24	37.30419525309984	39.0	35.0	41.0	32.0	41.0
25	37.15861253274387	39.0	35.0	41.0	32.0	41.0
26	37.4238720543545	39.0	35.0	41.0	33.0	41.0
27	37.51926856940398	39.0	35.0	41.0	33.0	41.0
28	37.448203141587676	39.0	35.0	41.0	33.0	41.0
29	37.48339262290918	39.0	35.0	41.0	33.0	41.0
30	37.30930792066182	39.0	35.0	41.0	32.0	41.0
31	37.31595373719716	39.0	35.0	41.0	33.0	41.0
32	37.21124109516596	39.0	35.0	41.0	32.0	41.0
33	37.14960642229201	39.0	35.0	41.0	32.0	41.0
34	37.071160516919996	39.0	35.0	41.0	32.0	41.0
35	36.98010944365059	39.0	35.0	41.0	32.0	41.0
36	36.88521051815746	39.0	35.0	41.0	31.0	41.0
37	36.88159322444545	39.0	35.0	41.0	32.0	41.0
38	36.75878629831145	39.0	35.0	41.0	31.0	41.0
39	36.696745217213476	39.0	35.0	41.0	31.0	41.0
40	36.65579307568962	39.0	35.0	41.0	31.0	41.0
41	36.549277648459494	39.0	35.0	41.0	31.0	41.0
42	36.57374811287207	39.0	35.0	41.0	31.0	41.0
43	36.48696562854549	39.0	35.0	41.0	31.0	41.0
44	36.437945241702174	39.0	35.0	40.0	31.0	41.0
45	36.361477971241406	39.0	35.0	40.0	31.0	41.0
46	36.26385142132158	38.0	35.0	40.0	31.0	41.0
47	36.22621176734171	38.0	35.0	40.0	31.0	41.0
48	36.17334352827468	38.0	35.0	40.0	31.0	41.0
49	36.13097807609994	38.0	35.0	40.0	31.0	41.0
50	36.02236808340747	38.0	35.0	40.0	30.0	41.0
51	35.07000642177098	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	11.0
10	18.0
11	18.0
12	19.0
13	14.0
14	23.0
15	34.0
16	84.0
17	116.0
18	235.0
19	432.0
20	778.0
21	1382.0
22	2098.0
23	2822.0
24	3686.0
25	4721.0
26	5909.0
27	6833.0
28	7938.0
29	9786.0
30	12289.0
31	16144.0
32	21872.0
33	32453.0
34	53372.0
35	65987.0
36	71499.0
37	107814.0
38	158434.0
39	180789.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.474607952835804	19.81187988552835	24.465775086915347	12.247737074720497
2	32.2863979596223	25.021460177855705	25.26999443793873	17.42214742458327
3	30.54509503048713	25.791812176876157	26.728114200710955	16.934978591925763
4	27.423697507232635	25.095577575123652	28.857068051233487	18.62365686641023
5	23.752997586299873	37.05570267591158	21.975743160423132	17.21555657736541
6	25.89080905196164	35.438666876817926	23.989157236997215	14.681366834223219
7	77.97996876388072	5.9825374722711055	11.079313430619472	4.958180333228692
8	77.82131324565162	6.500447439823577	9.594881340521896	6.083357974002899
9	71.64638316219467	7.0729359477192295	12.04153700464113	9.239143885444985
10	41.06937466539707	23.724731373282047	18.702072812201624	16.50382114911925
11	30.957755688738192	23.32861361389395	25.419792341028604	20.293838356339254
12	27.616350636510827	19.92507499664583	30.67105552812879	21.78751883871455
13	25.501464762974123	21.664945076273185	32.299293605192645	20.53429655556004
14	20.034101818286025	26.467596108380736	30.230779952090725	23.267522121242514
15	19.365872911459018	23.211250213299188	36.06430107554894	21.35857579969285
16	22.464084324496124	23.546927775266674	30.634713254248723	23.354274645988475
17	22.471899867266032	23.91373724926762	29.131393602457205	24.482969281009144
18	23.0372241276226	24.15875451510419	29.64383269007074	23.160188667202465
19	23.220889382715406	26.84651967367504	27.03396244110663	22.898628502502927
20	25.03904514908799	25.184544503654415	27.72928522953598	22.047125117721613
21	24.019637853799853	27.727982639074327	27.214501479091467	21.037878028034353
22	21.566469237372363	24.68513132065739	27.87595691551789	25.872442526452353
23	22.555135397765536	25.719778924346848	27.675488243469786	24.049597434417826
24	23.47033545612159	23.076692618610632	28.89979301837564	24.553178906892136
25	22.281070364634147	25.83102014977185	26.0081724525564	25.879737033037603
26	20.911526753254197	24.808616896421913	28.342414559835145	25.937441790488748
27	22.904880936718854	24.977693138344225	27.117849266836956	24.999576658099965
28	20.805105112537305	25.92324355445675	27.730197042859135	25.541454290146813
29	22.257623736324426	23.96909734388779	26.20681749795819	27.56646142182959
30	23.746875411130112	25.86462698368245	25.89445630525426	24.49404129993318
31	24.199525596553865	25.42786840189084	24.58183589704846	25.790770104506834
32	24.067442923742448	25.299172464279714	24.597336723542107	26.036047888435732
33	23.260357873703434	25.19535600448612	25.20538595104084	26.33890017076961
34	22.304126215805372	22.974309008324855	28.358045645374958	26.363519130494815
35	20.47867594284754	24.44011405482082	28.219059243116785	26.86215075921485
36	21.417843665697976	26.56333650731209	27.356744357503764	24.662075469486165
37	22.047125117721613	26.659337424335778	28.593163223702977	22.700374234239632
38	22.379415944488805	24.819167879161288	27.657251977006673	25.144164199343233
39	22.121112255943395	23.39765090836146	29.252404256344594	25.228832579350552
40	21.786085989206736	22.640455073003682	31.332120187416717	24.24133875037287
41	19.39752585967714	24.411066287526005	29.955412328497683	26.23599552429917
42	21.121895112810847	23.72759707229768	30.245368965261214	24.90513884963026
43	22.043868641567485	22.68526418488448	29.526860066614475	25.74400710693356
44	22.095190705756536	23.018206306882497	27.77201019667813	27.114592790682828
45	21.94317839888186	22.212944883489797	27.78542687843314	28.05844983919521
46	22.652048128112376	24.17412508255167	27.81577723618961	25.358049553146344
47	20.12033330684733	23.163184625264265	31.289525479320723	25.426956588567684
48	20.807970811552938	21.935493115158113	30.312191855943915	26.944344217345034
49	21.93301819328098	21.368084710062902	31.30724070959918	25.391656387056937
50	20.41875678161159	21.022246942494537	31.001522728249668	27.5574735476442
51	20.300611826739836	20.890294528729285	29.347232841952792	29.461860802578087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	327.0
1	659.5
2	992.0
3	3012.5
4	5033.0
5	3330.0
6	1627.0
7	1564.5
8	1502.0
9	1505.0
10	1508.0
11	1551.0
12	1594.0
13	1615.5
14	1637.0
15	1661.0
16	1685.0
17	1730.0
18	1775.0
19	1864.5
20	1954.0
21	2091.0
22	2228.0
23	2573.0
24	2918.0
25	3381.0
26	4705.5
27	5567.0
28	6673.5
29	7780.0
30	9185.5
31	10591.0
32	11816.5
33	13042.0
34	14258.5
35	15475.0
36	17254.5
37	19034.0
38	20560.0
39	22086.0
40	25153.0
41	28220.0
42	31975.5
43	35731.0
44	41307.0
45	46883.0
46	60033.5
47	73184.0
48	75570.5
49	77957.0
50	75483.5
51	73010.0
52	64635.5
53	56261.0
54	50774.0
55	45287.0
56	43003.5
57	40720.0
58	38622.5
59	36525.0
60	35368.5
61	34212.0
62	31622.0
63	29032.0
64	25089.0
65	21146.0
66	18015.0
67	14884.0
68	12450.0
69	10016.0
70	8770.5
71	7525.0
72	6378.0
73	5231.0
74	4596.0
75	3250.5
76	2540.0
77	1998.5
78	1457.0
79	1242.0
80	1027.0
81	723.0
82	419.0
83	286.0
84	153.0
85	102.5
86	52.0
87	47.5
88	43.0
89	25.5
90	8.0
91	10.0
92	12.0
93	8.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	767701.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.93751683795481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.64561046425324	26.10702849262607
2	9.910036432063775	7.12284202284075
3	3.7930025910606915	4.089332834479495
4	2.146946967807925	3.086237712231733
5	1.4465539309648996	2.59927781255304
6	1.1378342337673533	2.45345621608894
7	0.9103414456723645	2.2900787722497102
8	0.8212808178600838	2.3611834576428845
9	0.6977042832297415	2.2566383483833827
>10	6.38245125253932	40.31965921088811
>50	0.06944937063754492	1.7103127457255993
>100	0.032508214215183726	2.328696309045453
>500	0.003694115251725397	0.9335495379515172
>1k	0.002585880676207778	2.3417065272933466
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTC	3577	0.4659366081325933	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCG	2819	0.367200251139441	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGC	2795	0.3640740340314784	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2580	0.33606833910598005	No Hit
GCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	2366	0.30819290322664683	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGCT	2104	0.27406503313138836	TruSeq Adapter, Index 20 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCTGC	1398	0.18210214653882176	TruSeq Adapter, Index 23 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCCGTCTTCT	989	0.12882619665729236	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCAGCCTTTTCGTATGCC	806	0.10498879120907749	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2913894862713478	0.0
2	0.0	0.0	0.0	0.9795480271616163	0.0
3	0.0	0.0	0.0	1.4523883647409603	0.0
4	0.0	0.0	0.0	2.0993850470430546	0.0
5	0.0	0.0	0.0	3.7207193946601604	0.0
6	0.0	0.0	0.0	4.827400250878923	0.0
7	0.0	0.0	0.0	5.930433853805062	0.0
8	0.0	0.0	0.0	7.898648041359852	0.0
9	0.0	0.0	0.0	8.77372831349705	0.0
10	0.0	0.0	0.0	10.587976308484684	0.0
11	0.0	0.0	0.0	12.16801853846745	0.0
12	0.0	0.0	0.0	13.834031738919188	0.0
13	0.0	0.0	0.0	14.413033199123095	0.0
14	0.0	0.0	0.0	14.677198544745936	0.0
15	0.0	0.0	0.0	15.187423228574666	0.0
16	0.0	0.0	0.0	15.925992020330831	0.0
17	0.0	0.0	0.0	16.789088460220842	0.0
18	1.3025904616510855E-4	0.0	0.0	17.78114135581431	0.0
19	1.3025904616510855E-4	0.0	0.0	18.466955233873605	0.0
20	1.3025904616510855E-4	0.0	0.0	19.083992335557724	0.0
21	1.3025904616510855E-4	0.0	0.0	19.790387142911108	0.0
22	1.3025904616510855E-4	0.0	0.0	20.503555420665077	0.0
23	1.3025904616510855E-4	0.0	0.0	21.19744535958661	0.0
24	1.3025904616510855E-4	0.0	0.0	21.757038221911916	0.0
25	1.3025904616510855E-4	0.0	0.0	22.254237001124135	0.0
26	1.3025904616510855E-4	0.0	0.0	22.71939205497974	0.0
27	1.3025904616510855E-4	0.0	0.0	23.205779333360255	0.0
28	1.3025904616510855E-4	0.0	0.0	23.65035345792177	0.0
29	1.3025904616510855E-4	0.0	0.0	24.141690580056558	0.0
30	1.3025904616510855E-4	0.0	0.0	24.687085206349867	0.0
31	2.605180923302171E-4	0.0	0.0	25.170216008576254	0.0
32	3.9077713849532564E-4	0.0	0.0	25.656863805049102	0.0
33	3.9077713849532564E-4	0.0	0.0	26.126447666474316	0.0
34	5.210361846604342E-4	0.0	0.0	26.597073600268853	0.0
35	5.210361846604342E-4	0.0	0.0	27.076947926341113	0.0
36	5.210361846604342E-4	0.0	0.0	27.53454795551914	0.0
37	5.210361846604342E-4	0.0	0.0	28.004652853129016	0.0
38	5.210361846604342E-4	0.0	0.0	28.485048215385937	0.0
39	6.512952308255428E-4	0.0	0.0	28.993709790660688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGATAG	30	2.1645556E-6	45.000004	1
ACGTTAG	20	7.0318213E-4	45.0	1
ATTACCG	20	7.0318213E-4	45.0	1
TAATCGT	80	0.0	42.1875	21
CGTTATT	230	0.0	42.065216	1
TACGGGC	205	0.0	41.707317	4
TTTAGCG	55	6.002665E-11	40.909092	1
TACGGGT	110	0.0	40.909092	4
TGTTACG	55	6.002665E-11	40.909092	1
ACGGGAC	215	0.0	40.81395	5
TAACGCG	45	1.9268555E-8	40.0	1
TTAATCG	85	0.0	39.705883	20
TCATGCG	40	3.4562254E-7	39.375	1
ACGGGTA	75	0.0	39.0	5
CGTAAGG	75	0.0	39.0	2
CGTTTTT	1365	0.0	38.736263	1
TTGACGG	70	0.0	38.571426	2
TACGTTG	35	6.24558E-6	38.571426	1
ACATACG	135	0.0	38.333336	17
CGTTAGG	100	0.0	38.250004	2
>>END_MODULE