FastQCFastQC Report
Sat 18 Jun 2016
SRR3550049_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550049_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651646
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC84511.2968697728521315No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC71011.0897020775083404No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG69221.0622331756812748No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52810.810409332674489No Hit
GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT24250.37213456385829113No Hit
GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC23620.3624667380755809No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT19320.2964799906697808No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC18000.2762235937917213No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT15090.23156744612872635No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTT14930.22911212529502212No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA14110.21652860602228816No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT13620.20900918596906912No Hit
CCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC13210.20271742633270212No Hit
CGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG10760.16512032606660673No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCC9650.14808653778278388No Hit
GAATATGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC8000.12276604168520945No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTT7330.11248438569407317No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6650.10204927215083036No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC6590.10112852683819129No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC6530.10020778152555222No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG6520.10005432397344571No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCACG207.031034E-445.0000041
ACGTCCA207.031034E-445.00000423
AGTTACG207.031034E-445.0000041
CAATGCG207.031034E-445.0000041
CGGTATG207.031034E-445.0000041
TACGTCC207.031034E-445.00000422
CGTTATT3350.042.3134351
CGTTTTT23000.041.0869561
AACACGG451.9261279E-840.02
GCGTTAG403.4551522E-739.3750041
CGGGATA800.039.3750046
TGCGTAG403.4551522E-739.3750041
ATAATAC2800.039.3753
CGATGAA5350.039.1121519
GATGAAT5600.038.97321320
CAACGAC356.244127E-638.57142612
CGTAAGG700.038.5714262
CGATAGT356.244127E-638.57142610
TACGGGA2050.038.4146354
TAATACG2350.038.297874
Produced by FastQC (version 0.11.5)