##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651646
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87914603941404	33.0	31.0	34.0	30.0	34.0
2	31.98177538111183	33.0	31.0	34.0	30.0	34.0
3	31.941099922350478	33.0	31.0	34.0	30.0	34.0
4	35.68090343530076	37.0	35.0	37.0	33.0	37.0
5	31.77858377094312	37.0	35.0	37.0	0.0	37.0
6	33.31766480573808	37.0	35.0	37.0	17.0	37.0
7	35.146278807818966	37.0	35.0	37.0	32.0	37.0
8	35.232359287097594	37.0	35.0	37.0	33.0	37.0
9	37.26764685120449	39.0	37.0	39.0	34.0	39.0
10	36.96997449535484	39.0	37.0	39.0	33.0	39.0
11	36.9844685611513	39.0	37.0	39.0	33.0	39.0
12	36.99100892202208	39.0	37.0	39.0	33.0	39.0
13	36.89164055330655	39.0	37.0	39.0	33.0	39.0
14	37.93453961199793	40.0	37.0	41.0	33.0	41.0
15	38.05367791715134	40.0	37.0	41.0	33.0	41.0
16	38.009185969069094	40.0	37.0	41.0	33.0	41.0
17	37.92974713264564	40.0	37.0	41.0	33.0	41.0
18	37.88004376609386	39.0	37.0	41.0	33.0	41.0
19	37.802148712644595	39.0	37.0	41.0	33.0	41.0
20	37.71928777280916	39.0	36.0	41.0	33.0	41.0
21	37.656328742906425	39.0	36.0	41.0	33.0	41.0
22	37.645335043873516	39.0	36.0	41.0	33.0	41.0
23	37.386488983282334	39.0	35.0	41.0	32.0	41.0
24	37.150142868981014	39.0	35.0	41.0	32.0	41.0
25	37.00267169598217	39.0	35.0	40.0	31.0	41.0
26	37.24918130395951	39.0	35.0	41.0	32.0	41.0
27	37.34959164945384	39.0	35.0	41.0	33.0	41.0
28	37.24354020434408	39.0	35.0	41.0	32.0	41.0
29	37.21830257532464	39.0	35.0	41.0	32.0	41.0
30	37.06110065894673	39.0	35.0	41.0	32.0	41.0
31	37.05980240805591	39.0	35.0	41.0	32.0	41.0
32	36.862360852364624	39.0	35.0	41.0	31.0	41.0
33	36.690744668117354	39.0	35.0	41.0	31.0	41.0
34	36.55778904497227	39.0	35.0	41.0	31.0	41.0
35	36.454194455271725	39.0	35.0	41.0	30.0	41.0
36	36.33069642106297	39.0	35.0	41.0	30.0	41.0
37	36.32664360711183	39.0	35.0	41.0	30.0	41.0
38	36.173207539062616	39.0	35.0	40.0	30.0	41.0
39	36.0596121206913	39.0	35.0	40.0	30.0	41.0
40	35.964141573799274	39.0	35.0	40.0	29.0	41.0
41	35.827475347044256	38.0	35.0	40.0	29.0	41.0
42	35.90653667788953	39.0	35.0	40.0	29.0	41.0
43	35.79572037578686	38.0	35.0	40.0	29.0	41.0
44	35.77027250992103	38.0	35.0	40.0	29.0	41.0
45	35.717285151754176	38.0	35.0	40.0	28.0	41.0
46	35.606840216927594	38.0	35.0	40.0	28.0	41.0
47	35.46859337738588	38.0	35.0	40.0	28.0	41.0
48	35.428442743452734	38.0	35.0	40.0	28.0	41.0
49	35.407498856741235	38.0	35.0	40.0	28.0	41.0
50	35.27898582972964	37.0	34.0	40.0	27.0	41.0
51	34.19339181089119	36.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	5.0
9	6.0
10	16.0
11	17.0
12	6.0
13	19.0
14	31.0
15	35.0
16	56.0
17	136.0
18	234.0
19	466.0
20	948.0
21	1498.0
22	2435.0
23	3254.0
24	4296.0
25	5543.0
26	6947.0
27	7953.0
28	8472.0
29	9694.0
30	11555.0
31	14915.0
32	19542.0
33	27997.0
34	43424.0
35	54935.0
36	64205.0
37	93383.0
38	133862.0
39	135701.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.86245292689589	19.603281536294244	23.731750060615735	14.80251547619413
2	32.76349428984449	29.202204878108667	21.813070286628015	16.221230545418834
3	28.228670167544955	28.700398682720373	27.23104262130052	15.839888528434152
4	25.969161170328675	22.948349257111992	33.1526626419866	17.929826930572734
5	25.76966635259021	34.35362144477216	22.858883504233894	17.017828698403733
6	24.750401291498758	35.46818364572176	25.522753151250832	14.258661911528653
7	72.53432078152862	5.554242640943088	16.74651574627942	5.164920831248868
8	71.86110250043735	9.191493540971631	12.708126805044456	6.239277153546557
9	65.76039751644298	6.568750517919239	14.788397381400332	12.882454584237454
10	39.48800422315183	21.678027640774285	21.411471872765276	17.422496263308606
11	29.583239979989134	21.92202514862364	27.531972880981392	20.962761990405834
12	24.186137872403116	18.51419328899433	33.06104848337901	24.238620355223542
13	24.164807272660312	19.81781519413915	36.47486518754047	19.542512345660068
14	19.7341808282411	26.483397427437595	31.734101030314005	22.0483207140073
15	17.932896081614867	22.503015440898892	38.08202613075197	21.48206234673427
16	20.084677877252375	23.34258170847362	31.147739723715024	25.425000690558985
17	20.278034392906576	24.23616503438984	30.68798089760391	24.79781967509967
18	21.52426317356356	22.919192322211753	31.021137243227155	24.535407260997534
19	21.65930581941729	24.682879968571893	28.708992305638336	24.94882190637248
20	23.22257790272633	23.90791933043401	30.626444419209204	22.243058347630463
21	22.022079472597085	27.1174840327417	29.555157247953645	21.305279246707567
22	20.166470752525147	22.49165958204301	30.264744968894153	27.07712469653769
23	21.263845707638808	25.674062297627852	29.057954779128547	24.004137215604793
24	22.65371075706749	22.782001270628534	28.83866393716834	25.72562403513564
25	21.467330421732044	27.50250903097694	26.502579621450913	24.527580925840102
26	20.220180895762425	23.89011825438965	29.628203042756347	26.261497807091583
27	23.679727950451625	23.79343999656255	26.836963627491002	25.689868425494822
28	20.244887561651574	24.5180665576095	29.64677140656123	25.590274474177697
29	23.118533682398112	24.25059004428785	26.377665174036206	26.253211099277827
30	22.284184971595007	25.933559018239965	26.4316822323777	25.35057377778733
31	22.957403252686273	24.5369418365186	23.71594393274876	28.78971097804636
32	25.01511556888249	26.783406941805826	23.020167391497836	25.181310097813842
33	22.318712920818975	25.489299404891614	23.874619041626897	28.31736863266252
34	21.72882209052154	25.32233758819973	26.88791153479036	26.06092878648837
35	21.271518585244138	24.61428444278028	25.149237469423586	28.964959502552002
36	19.970198543380917	29.74176163131516	24.52159608130795	25.766443743995975
37	21.762122379328655	25.075270929308246	27.42792866065318	25.734678030709922
38	21.30650690712442	26.300322567774526	24.4213882997824	27.971782225318652
39	24.344352608624927	25.274612289494602	27.122087759304897	23.25894734257557
40	21.446153279541345	24.422462502647143	29.440217541425866	24.691166676385645
41	19.25554672322089	26.254285302142577	27.57294604739383	26.917221927242707
42	20.922863026858142	24.28588528127235	28.372152978764543	26.419098713104965
43	22.64956740316061	24.409265153165983	26.957581263446716	25.983586180226688
44	22.86901170267292	22.72737038207861	27.17349603926058	27.230121875987884
45	21.96453289055714	21.323847610512455	27.377287668458028	29.334331830472372
46	25.10181908582267	23.9171267835604	26.12691553389417	24.85413859672276
47	19.842982232684616	23.003900890974545	31.958762886597935	25.194353989742897
48	21.05575726698238	23.001905942797162	28.335630081363195	27.606706708857264
49	21.439247689696554	21.265840655816195	31.446675035218508	25.848236619268743
50	21.20783370111993	21.07831552714204	30.738468432246957	26.975382339491073
51	20.94051064535039	21.241287447479156	27.772440865132296	30.045761042038162
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	293.0
1	580.0
2	867.0
3	4975.5
4	9084.0
5	5883.5
6	2683.0
7	2477.0
8	2271.0
9	2279.5
10	2288.0
11	2292.5
12	2297.0
13	2243.0
14	2189.0
15	2123.5
16	2058.0
17	1946.5
18	1835.0
19	1850.5
20	1866.0
21	1869.0
22	1872.0
23	2119.5
24	2367.0
25	2812.0
26	3701.0
27	4145.0
28	5044.5
29	5944.0
30	6424.5
31	6905.0
32	7898.0
33	8891.0
34	9241.5
35	9592.0
36	10681.0
37	11770.0
38	13287.5
39	14805.0
40	16953.0
41	19101.0
42	22686.0
43	26271.0
44	32383.5
45	38496.0
46	61596.0
47	84696.0
48	76374.5
49	68053.0
50	65654.0
51	63255.0
52	54728.0
53	46201.0
54	41216.0
55	36231.0
56	33793.0
57	31355.0
58	29746.5
59	28138.0
60	27500.0
61	26862.0
62	24568.5
63	22275.0
64	20409.0
65	18543.0
66	15953.5
67	13364.0
68	11666.0
69	9968.0
70	8801.5
71	7635.0
72	6490.5
73	5346.0
74	4608.0
75	2979.5
76	2089.0
77	1706.5
78	1324.0
79	1008.0
80	692.0
81	506.5
82	321.0
83	235.0
84	149.0
85	113.5
86	78.0
87	52.5
88	27.0
89	20.0
90	13.0
91	11.5
92	10.0
93	6.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651646.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.3101737706338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.51639537017981	29.634586723625667
2	9.14772728065364	7.374929525790313
3	3.821227805513775	4.621030705723138
4	2.3955183399571487	3.8625504221765152
5	1.8500899995032998	3.728872468564491
6	1.5367271938646636	3.7167444133645837
7	1.2511007596056203	3.5302462316992154
8	1.0731375542929118	3.46066890346722
9	0.9843093050676655	3.5709911218196457
>10	4.336518018842554	24.932992261988453
>50	0.04924685946354895	1.335989953137106
>100	0.029082790603615474	2.297302459277537
>500	0.003489934872433856	0.9856970339663975
>1k	0.003877705413815395	2.6089865515276154
>5k	0.0015510821655261582	4.338411223872084
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	8451	1.2968697728521315	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC	7101	1.0897020775083404	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG	6922	1.0622331756812748	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5281	0.810409332674489	No Hit
GAACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCT	2425	0.37213456385829113	No Hit
GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	2362	0.3624667380755809	No Hit
GAATGACTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCT	1932	0.2964799906697808	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTC	1800	0.2762235937917213	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACCGATCAT	1509	0.23156744612872635	No Hit
GAATGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTT	1493	0.22911212529502212	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACCGATCATCGTA	1411	0.21652860602228816	No Hit
CTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGCT	1362	0.20900918596906912	No Hit
CCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC	1321	0.20271742633270212	No Hit
CGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTG	1076	0.16512032606660673	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCC	965	0.14808653778278388	No Hit
GAATATGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	800	0.12276604168520945	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTT	733	0.11248438569407317	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	665	0.10204927215083036	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGC	659	0.10112852683819129	No Hit
CGTTCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTC	653	0.10020778152555222	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCG	652	0.10005432397344571	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22266690810654866	0.0
2	0.0	0.0	0.0	0.9878062629096166	0.0
3	0.0	0.0	0.0	1.5640393710695684	0.0
4	0.0	0.0	0.0	2.536806793872747	0.0
5	0.0	0.0	0.0	5.141288368224465	0.0
6	0.0	0.0	0.0	6.7489096840922835	0.0
7	0.0	0.0	0.0	8.265684129113046	0.0
8	0.0	0.0	0.0	10.416238264333703	0.0
9	0.0	0.0	0.0	11.363838648591413	0.0
10	0.0	0.0	0.0	13.965711444557321	0.0
11	0.0	0.0	0.0	15.624587582828713	0.0
12	0.0	0.0	0.0	18.174131353526302	0.0
13	0.0	0.0	0.0	18.86760603149563	0.0
14	0.0	0.0	0.0	19.19968817425412	0.0
15	0.0	0.0	0.0	19.818582481899682	0.0
16	0.0	0.0	0.0	20.574207468472146	0.0
17	0.0	0.0	0.0	21.42313464672537	0.0
18	0.0	0.0	0.0	22.377794078379978	0.0
19	0.0	0.0	0.0	23.230097322779546	0.0
20	1.534575521065118E-4	0.0	0.0	23.866332333813144	0.0
21	1.534575521065118E-4	0.0	0.0	24.496429042762482	0.0
22	1.534575521065118E-4	0.0	0.0	25.178701319428033	0.0
23	1.534575521065118E-4	0.0	0.0	25.77979455102924	0.0
24	1.534575521065118E-4	0.0	0.0	26.274541699020634	0.0
25	1.534575521065118E-4	0.0	0.0	26.73921116679915	0.0
26	1.534575521065118E-4	0.0	0.0	27.189915997335977	0.0
27	1.534575521065118E-4	0.0	0.0	27.669163932564615	0.0
28	1.534575521065118E-4	0.0	0.0	28.113730461017177	0.0
29	1.534575521065118E-4	0.0	0.0	28.577939556139377	0.0
30	1.534575521065118E-4	0.0	0.0	29.111511464813717	0.0
31	1.534575521065118E-4	0.0	0.0	29.574492899519065	0.0
32	1.534575521065118E-4	0.0	0.0	30.038855452193367	0.0
33	1.534575521065118E-4	0.0	0.0	30.518103387422006	0.0
34	1.534575521065118E-4	0.0	0.0	30.961135340353504	0.0
35	1.534575521065118E-4	0.0	0.0	31.412454001098755	0.0
36	1.534575521065118E-4	0.0	0.0	31.836610675121154	0.0
37	1.534575521065118E-4	0.0	0.0	32.25693091034089	0.0
38	1.534575521065118E-4	0.0	0.0	32.684924023165955	0.0
39	1.534575521065118E-4	0.0	0.0	33.14360864641232	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	20	7.031034E-4	45.000004	1
ACGTCCA	20	7.031034E-4	45.000004	23
AGTTACG	20	7.031034E-4	45.000004	1
CAATGCG	20	7.031034E-4	45.000004	1
CGGTATG	20	7.031034E-4	45.000004	1
TACGTCC	20	7.031034E-4	45.000004	22
CGTTATT	335	0.0	42.313435	1
CGTTTTT	2300	0.0	41.086956	1
AACACGG	45	1.9261279E-8	40.0	2
GCGTTAG	40	3.4551522E-7	39.375004	1
CGGGATA	80	0.0	39.375004	6
TGCGTAG	40	3.4551522E-7	39.375004	1
ATAATAC	280	0.0	39.375	3
CGATGAA	535	0.0	39.11215	19
GATGAAT	560	0.0	38.973213	20
CAACGAC	35	6.244127E-6	38.571426	12
CGTAAGG	70	0.0	38.571426	2
CGATAGT	35	6.244127E-6	38.571426	10
TACGGGA	205	0.0	38.414635	4
TAATACG	235	0.0	38.29787	4
>>END_MODULE