FastQCFastQC Report
Sat 18 Jun 2016
SRR3550047_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550047_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288529
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC216501.6802105346484246No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG209691.6273595704869661No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC209431.6253417656878502No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC71840.5575349875710985No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT51400.39890448720983385No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT48050.3729058484519945No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47730.3704223963915441No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT45470.35288301621461377No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTCGCTT43600.3383703432363571No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTA42250.3278932798563323No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC40120.31136280207895983No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC38420.29816946300781744No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG21710.1684867007261769No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC21650.16802105346484247No Hit
GAATATGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC19230.14923994725768686No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC19130.14846386848879614No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG18700.14512672978256602No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT18020.13984939415410907No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAGCG253.891069E-545.0000041
GATTACG253.891069E-545.0000041
GGTACGT207.0336054E-445.09
TATAGCG351.2120836E-745.01
ACGTAAG351.2120836E-745.01
CCGCAAT351.2120836E-745.042
TAATACG6200.044.2741974
CCGATGA17450.042.6790818
CGATGAA17750.042.59155319
ATAATAC9150.041.5573773
CGATCGC650.041.5384634
CGAATAT556.184564E-1140.90909214
CGTAAGG2350.040.2127652
CGTAAGC451.9286745E-840.00000443
GATGAAT19750.039.87341720
TACGGCT29500.039.737297
AATACGG7000.039.5357135
ACGGCTG29850.039.422118
GCGTAAG1200.039.3750041
CACGACG800.039.37526
Produced by FastQC (version 0.11.5)