##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1288529
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896279400774063	33.0	31.0	34.0	30.0	34.0
2	31.993901573034055	33.0	31.0	34.0	30.0	34.0
3	31.981526220985327	33.0	31.0	34.0	30.0	34.0
4	35.701545716084	37.0	35.0	37.0	35.0	37.0
5	31.758960023406537	37.0	35.0	37.0	0.0	37.0
6	33.30870085190166	37.0	35.0	37.0	17.0	37.0
7	35.16269715311025	37.0	35.0	37.0	32.0	37.0
8	35.19712633553455	37.0	35.0	37.0	32.0	37.0
9	37.27813266135259	39.0	37.0	39.0	34.0	39.0
10	36.987756581341976	39.0	37.0	39.0	33.0	39.0
11	37.03814194325467	39.0	37.0	39.0	33.0	39.0
12	37.03218321046713	39.0	37.0	39.0	33.0	39.0
13	36.88884146185301	39.0	37.0	39.0	33.0	39.0
14	37.939413082670235	40.0	37.0	41.0	33.0	41.0
15	38.02308523905943	40.0	37.0	41.0	33.0	41.0
16	38.02912390796016	40.0	37.0	41.0	33.0	41.0
17	37.91959435914907	40.0	37.0	41.0	33.0	41.0
18	37.92693916861786	40.0	37.0	41.0	33.0	41.0
19	37.89964525439474	40.0	37.0	41.0	33.0	41.0
20	37.78675761275066	40.0	36.0	41.0	33.0	41.0
21	37.83450353077036	40.0	36.0	41.0	33.0	41.0
22	37.82396050069498	40.0	36.0	41.0	33.0	41.0
23	37.52850731337828	39.0	36.0	41.0	32.0	41.0
24	37.320489488401115	39.0	36.0	41.0	32.0	41.0
25	37.19612441784391	39.0	35.0	41.0	32.0	41.0
26	37.41691029072687	39.0	36.0	41.0	32.0	41.0
27	37.50168835936172	39.0	36.0	41.0	33.0	41.0
28	37.432414792371766	39.0	36.0	41.0	33.0	41.0
29	37.433519928538665	39.0	36.0	41.0	33.0	41.0
30	37.313940935749216	39.0	36.0	41.0	32.0	41.0
31	37.2873330751578	39.0	36.0	41.0	32.0	41.0
32	37.113442537963834	39.0	36.0	41.0	32.0	41.0
33	37.082230202036584	39.0	36.0	41.0	32.0	41.0
34	36.90859577083636	39.0	35.0	41.0	31.0	41.0
35	36.905995130881806	39.0	35.0	41.0	31.0	41.0
36	36.74577444512308	39.0	35.0	41.0	31.0	41.0
37	36.750988918371256	39.0	35.0	41.0	31.0	41.0
38	36.6350924193402	39.0	35.0	41.0	31.0	41.0
39	36.579945038101584	39.0	35.0	41.0	31.0	41.0
40	36.56820451848581	39.0	35.0	41.0	31.0	41.0
41	36.37603810236324	39.0	35.0	40.0	30.0	41.0
42	36.48382845865324	39.0	35.0	41.0	31.0	41.0
43	36.366039103504846	39.0	35.0	40.0	30.0	41.0
44	36.38573986305314	39.0	35.0	40.0	31.0	41.0
45	36.33002051176186	39.0	35.0	40.0	30.0	41.0
46	36.234500736886794	39.0	35.0	40.0	30.0	41.0
47	36.096962505306436	39.0	35.0	40.0	30.0	41.0
48	36.05413692668151	38.0	35.0	40.0	30.0	41.0
49	36.05883530754838	38.0	35.0	40.0	30.0	41.0
50	35.92698107687138	38.0	35.0	40.0	30.0	41.0
51	34.93377254217794	37.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	4.0
9	19.0
10	21.0
11	22.0
12	33.0
13	24.0
14	33.0
15	62.0
16	102.0
17	197.0
18	380.0
19	844.0
20	1640.0
21	3124.0
22	5314.0
23	7042.0
24	8492.0
25	9362.0
26	10454.0
27	11625.0
28	12940.0
29	15360.0
30	19860.0
31	26285.0
32	36683.0
33	52019.0
34	80343.0
35	101137.0
36	122651.0
37	184508.0
38	275109.0
39	302733.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.54633306662093	19.161229588158278	22.451881176131852	11.840556169088938
2	29.81601500625908	31.991053363952226	22.203070322825486	15.989861306963213
3	28.990500019790012	31.059836449160244	24.700724624746513	15.248938906303234
4	26.2434140015475	22.656145108103892	33.50952908316383	17.59091180718478
5	27.30966862212647	34.70080999341109	20.598993115405243	17.390528269057196
6	24.0857598082775	38.21272163839541	23.361678316902452	14.339840236424637
7	72.1010547686548	5.639686805652026	17.043233019978597	5.2160254057145785
8	70.59204721042367	11.745952167161159	11.1774744689487	6.484526153466471
9	64.3183816584648	7.800290098243812	12.70828984058566	15.173038402705721
10	37.829183510809614	25.680834501978612	19.517372135202233	16.97260985200954
11	31.075513240291837	22.450872273732294	25.573735631871692	20.899878854104177
12	25.8968172233609	18.55022277341061	30.524342098625638	25.028617904602847
13	25.139519560677332	20.2804127807756	34.99044258996111	19.58962506858596
14	20.053176917244393	28.55488700681164	28.752088621986776	22.639847453957188
15	17.696303304000143	23.387288916275846	36.447763302184114	22.468644477539893
16	19.456217128213645	24.391534843220448	28.931750856984976	27.220497171580927
17	19.27570120656966	25.734616760662743	30.47327611563263	24.516405917134964
18	19.19638595638903	24.86106249839934	30.114029253513113	25.82852229169852
19	20.700116178991703	26.495717209313874	27.507180668809163	25.296985942885257
20	23.527448741937512	25.601441643921092	30.578124357309772	20.292985256831628
21	21.86539845048113	29.55207061695934	28.394083485897486	20.188447446662046
22	19.48182772758704	23.416547085863026	29.791723740792797	27.30990144575714
23	21.679217153824247	27.14645925702875	28.482711681304806	22.691611907842198
24	22.751680404554342	24.596652461838268	26.178689032222014	26.47297810138538
25	20.079718811140456	30.98820437879163	24.61163078207786	24.320446027990055
26	19.971766254387756	25.815794599888708	28.460283004883863	25.75215614083967
27	24.638483107481477	25.847303397905673	25.500396188211518	24.01381730640133
28	19.114199214763502	26.592183800286996	30.387829843177762	23.905787141771743
29	22.546950825320966	25.024271863497056	27.622273150235653	24.806504160946318
30	24.215830609943588	26.492302462730756	27.293526183733547	21.998340743592113
31	23.970822542604783	23.977419212140354	26.272516955380905	25.779241289873955
32	24.551795108996384	26.460095193821793	26.573014654695392	22.41509504248643
33	23.561363384138037	22.203768793717487	25.74377449013565	28.491093332008827
34	24.35257568902213	24.083198748340163	27.124573835746034	24.43965172689167
35	23.95033406310607	21.866640176511353	26.196150804522055	27.986874955860518
36	21.783987787624493	26.34965918500864	28.787322598094416	23.07903042927245
37	24.06666827056279	22.914656946021395	31.65004435290164	21.368630430514177
38	20.46993121613871	22.413853316456205	28.841570504039876	28.274644963365205
39	24.899711221090097	21.715304816577664	29.930098585286014	23.454885377046228
40	21.99508121276277	21.009538784148436	29.365578888794897	27.629801114293894
41	22.296898245984377	23.568968956073167	27.98097675721695	26.153156040725513
42	20.94791812989851	22.317619549113758	31.09701062219011	25.63745169879762
43	23.08135866557912	21.261453952530367	30.661397609211743	24.995789772678766
44	23.373319498435812	20.96328448952255	29.361931318581107	26.30146469346053
45	21.334094925298537	19.881353077811987	29.014325637994954	29.770226358894526
46	25.743464058628096	23.10239040021606	27.556849710018167	23.59729583113768
47	19.737468074059645	21.747977732747962	34.22305590328196	24.291498289910432
48	21.866484960757575	22.2654670558443	28.93182846486187	26.93621951853625
49	21.236929863433417	19.79916633618646	33.96881249859336	24.995091301786765
50	21.699938456953628	19.292309292223926	31.31710656104752	27.69064568977493
51	21.161805438604798	19.493779340627956	28.102510692425238	31.24190452834201
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	744.0
1	1404.0
2	2064.0
3	5517.0
4	8970.0
5	5932.5
6	2895.0
7	2805.0
8	2715.0
9	2707.5
10	2700.0
11	2829.5
12	2959.0
13	2959.5
14	2960.0
15	2895.0
16	2830.0
17	2777.0
18	2724.0
19	3008.0
20	3292.0
21	3455.5
22	3619.0
23	3948.5
24	4278.0
25	5331.5
26	7687.5
27	8990.0
28	10811.5
29	12633.0
30	15144.0
31	17655.0
32	19511.0
33	21367.0
34	22539.0
35	23711.0
36	26327.0
37	28943.0
38	31070.5
39	33198.0
40	38423.5
41	43649.0
42	49461.0
43	55273.0
44	68115.5
45	80958.0
46	134097.0
47	187236.0
48	162682.0
49	138128.0
50	133217.5
51	128307.0
52	107948.5
53	87590.0
54	77497.0
55	67404.0
56	63253.0
57	59102.0
58	55837.0
59	52572.0
60	50499.0
61	48426.0
62	45081.0
63	41736.0
64	36335.0
65	30934.0
66	26739.5
67	22545.0
68	19192.5
69	15840.0
70	13512.0
71	11184.0
72	10109.0
73	9034.0
74	7396.0
75	4670.0
76	3582.0
77	2691.0
78	1800.0
79	1348.5
80	897.0
81	712.5
82	528.0
83	394.5
84	261.0
85	184.0
86	107.0
87	67.0
88	27.0
89	17.5
90	8.0
91	7.0
92	6.0
93	5.0
94	4.0
95	2.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1288529.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.879324033415795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80925545612888	22.174212678281354
2	11.152758648649106	6.887792963562325
3	4.246453739075579	3.9338286300547467
4	2.2061490341151924	2.724975635618012
5	1.3464960688944976	2.0789444210556876
6	0.977942562931498	1.8118923172098507
7	0.7929055531415229	1.713907125234631
8	0.6485893847759346	1.6022401421703907
9	0.5188445664841844	1.4419412542298045
>10	6.0993125531928705	38.432218381792325
>50	0.1327427210002639	2.706029186598168
>100	0.0567396489646668	3.39895980731537
>500	0.005648290830626799	1.1932391942958782
>1k	0.004878069353723145	3.883596782391727
>5k	5.134809846024363E-4	0.9770433745599895
>10k+	7.702214769036544E-4	5.039178105629832
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	21650	1.6802105346484246	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	20969	1.6273595704869661	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	20943	1.6253417656878502	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	7184	0.5575349875710985	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	5140	0.39890448720983385	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	4805	0.3729058484519945	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4773	0.3704223963915441	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	4547	0.35288301621461377	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTCGCTT	4360	0.3383703432363571	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTA	4225	0.3278932798563323	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC	4012	0.31136280207895983	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	3842	0.29816946300781744	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	2171	0.1684867007261769	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	2165	0.16802105346484247	No Hit
GAATATGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	1923	0.14923994725768686	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCC	1913	0.14846386848879614	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTG	1870	0.14512672978256602	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTT	1802	0.13984939415410907	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3729058484519945	0.0
2	0.0	0.0	0.0	1.4694275410177031	0.0
3	0.0	0.0	0.0	2.051952264947083	0.0
4	0.0	0.0	0.0	3.05239540592412	0.0
5	0.0	0.0	0.0	6.112706815290925	0.0
6	0.0	0.0	0.0	7.3361949944471565	0.0
7	0.0	0.0	0.0	8.774036129571007	0.0
8	0.0	0.0	0.0	10.695762377098227	0.0
9	0.0	0.0	0.0	11.330206770666395	0.0
10	0.0	0.0	0.0	14.436850082535978	0.0
11	0.0	0.0	0.0	16.242397338360256	0.0
12	0.0	0.0	0.0	19.60980311657712	0.0
13	0.0	0.0	0.0	20.324261231217925	0.0
14	0.0	0.0	0.0	20.670858009404522	0.0
15	0.0	0.0	0.0	21.484964637970897	0.0
16	0.0	0.0	0.0	22.362244078324974	0.0
17	0.0	0.0	0.0	23.346311957278417	0.0
18	0.0	0.0	0.0	24.358551495542592	0.0
19	0.0	0.0	0.0	25.439474004853597	0.0
20	0.0	0.0	0.0	26.15059498078817	0.0
21	0.0	0.0	0.0	26.897648403722386	0.0
22	0.0	0.0	0.0	27.727975078558572	0.0
23	0.0	0.0	0.0	28.46199037817542	0.0
24	0.0	0.0	0.0	29.07842974430533	0.0
25	0.0	0.0	0.0	29.601429226660787	0.0
26	0.0	0.0	0.0	30.085391946941048	0.0
27	0.0	0.0	0.0	30.611340528618292	0.0
28	0.0	0.0	0.0	31.11532608113593	0.0
29	0.0	0.0	0.0	31.6233472432518	0.0
30	0.0	0.0	0.0	32.21270146034742	0.0
31	0.0	0.0	0.0	32.718704817664175	0.0
32	0.0	0.0	0.0	33.22672597978004	0.0
33	0.0	0.0	0.0	33.69369257502159	0.0
34	0.0	0.0	0.0	34.18153568914631	0.0
35	0.0	0.0	0.0	34.69304920572218	0.0
36	0.0	0.0	0.0	35.170725687974425	0.0
37	0.0	0.0	0.0	35.6652430795116	0.0
38	0.0	0.0	0.0	36.1439284641634	0.0
39	0.0	0.0	0.0	36.643102328313915	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.891069E-5	45.000004	1
GATTACG	25	3.891069E-5	45.000004	1
GGTACGT	20	7.0336054E-4	45.0	9
TATAGCG	35	1.2120836E-7	45.0	1
ACGTAAG	35	1.2120836E-7	45.0	1
CCGCAAT	35	1.2120836E-7	45.0	42
TAATACG	620	0.0	44.274197	4
CCGATGA	1745	0.0	42.67908	18
CGATGAA	1775	0.0	42.591553	19
ATAATAC	915	0.0	41.557377	3
CGATCGC	65	0.0	41.53846	34
CGAATAT	55	6.184564E-11	40.909092	14
CGTAAGG	235	0.0	40.212765	2
CGTAAGC	45	1.9286745E-8	40.000004	43
GATGAAT	1975	0.0	39.873417	20
TACGGCT	2950	0.0	39.73729	7
AATACGG	700	0.0	39.535713	5
ACGGCTG	2985	0.0	39.42211	8
GCGTAAG	120	0.0	39.375004	1
CACGACG	80	0.0	39.375	26
>>END_MODULE