FastQCFastQC Report
Sat 18 Jun 2016
SRR3550041_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550041_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences815013
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC63800.782809599356084No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC60360.7406016836541258No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC55690.6833019841401303Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG51130.6273519563491625No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT50700.6220759668864178Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC32560.3995028300162083Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23050.2828175746889927No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC16360.2007329944430334Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT14120.17324877026501417No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT13330.16355567334508775No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC13260.16269679133952464Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC13250.16257409391015848No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA13140.16122442218713076No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT12570.15423066871325977No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC12020.14748231009812113No Hit
AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG9050.1110411735763724No Hit
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG8670.10637867126045843No Hit
GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG8420.1033112355263045No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCATCG600.045.00000443
TATCACG302.164721E-645.0000041
TATACGG600.045.0000042
TTAATCG253.889804E-545.020
CTATACG207.032078E-445.01
GTATACG207.032078E-445.01
ATGCGAG453.8380676E-1045.01
CGGTCTA351.2113378E-745.031
TTTGACG453.8380676E-1045.01
TATCCGT207.032078E-445.026
TCATACG650.044.99999631
CGTAAGG1600.042.18752
ATAGCGG750.042.0000042
TCTACGG501.0804797E-940.52
TGCGATG501.0804797E-940.51
GATAGCG501.0804797E-940.51
TATGGGT1450.040.3448264
GCGTAAG950.040.2631571
TAATACG2100.039.6428574
TCACGAC403.456571E-739.37525
Produced by FastQC (version 0.11.5)