##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	815013
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.8095011981404	31.0	31.0	34.0	30.0	34.0
2	31.895099832763403	33.0	31.0	34.0	30.0	34.0
3	31.88686928920152	33.0	31.0	34.0	30.0	34.0
4	35.62372870125998	37.0	35.0	37.0	33.0	37.0
5	31.403154305514146	37.0	35.0	37.0	0.0	37.0
6	33.12571701310286	36.0	35.0	37.0	17.0	37.0
7	35.092469690667514	37.0	35.0	37.0	32.0	37.0
8	35.21981244470947	37.0	35.0	37.0	33.0	37.0
9	37.27194412849856	39.0	37.0	39.0	34.0	39.0
10	36.93483784921222	39.0	37.0	39.0	33.0	39.0
11	37.02379839339986	39.0	37.0	39.0	33.0	39.0
12	37.058784338409325	39.0	37.0	39.0	33.0	39.0
13	36.9704029260883	39.0	37.0	39.0	33.0	39.0
14	37.956227692073625	40.0	37.0	41.0	33.0	41.0
15	38.103565219205095	40.0	37.0	41.0	33.0	41.0
16	38.01938742081415	40.0	37.0	41.0	33.0	41.0
17	37.997986535184104	40.0	37.0	41.0	33.0	41.0
18	37.99722949204491	40.0	37.0	41.0	33.0	41.0
19	37.97886536779168	40.0	37.0	41.0	33.0	41.0
20	37.99838530182954	40.0	37.0	41.0	33.0	41.0
21	37.89256858479558	40.0	37.0	41.0	33.0	41.0
22	37.892020127286315	40.0	37.0	41.0	33.0	41.0
23	37.64050634775151	39.0	36.0	41.0	33.0	41.0
24	37.36738064300815	39.0	36.0	41.0	32.0	41.0
25	37.22398415730792	39.0	36.0	41.0	32.0	41.0
26	37.548628058693545	39.0	36.0	41.0	33.0	41.0
27	37.68061122951413	39.0	36.0	41.0	33.0	41.0
28	37.61867111322151	39.0	36.0	41.0	33.0	41.0
29	37.66416854700477	40.0	36.0	41.0	33.0	41.0
30	37.53240500458275	40.0	36.0	41.0	33.0	41.0
31	37.52994860204684	40.0	36.0	41.0	33.0	41.0
32	37.405137095972705	39.0	36.0	41.0	33.0	41.0
33	37.36761867602112	39.0	36.0	41.0	33.0	41.0
34	37.29787009532363	40.0	36.0	41.0	32.0	41.0
35	37.242992443065326	40.0	36.0	41.0	32.0	41.0
36	37.1687212351214	39.0	36.0	41.0	32.0	41.0
37	37.10814919516621	39.0	35.0	41.0	32.0	41.0
38	37.00887593204035	39.0	35.0	41.0	32.0	41.0
39	36.88794657263136	39.0	35.0	41.0	31.0	41.0
40	36.8371829651797	39.0	35.0	41.0	31.0	41.0
41	36.691563202059356	39.0	35.0	40.0	31.0	41.0
42	36.7591326764113	39.0	35.0	40.0	31.0	41.0
43	36.68726265716007	39.0	35.0	40.0	31.0	41.0
44	36.66573293922919	39.0	35.0	40.0	31.0	41.0
45	36.616002444132796	39.0	35.0	40.0	31.0	41.0
46	36.537910438238406	39.0	35.0	40.0	31.0	41.0
47	36.43893042196873	39.0	35.0	40.0	31.0	41.0
48	36.424255809416536	39.0	35.0	40.0	31.0	41.0
49	36.420206794247456	39.0	35.0	40.0	31.0	41.0
50	36.311022032777394	39.0	35.0	40.0	31.0	41.0
51	35.227801274335505	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	14.0
10	20.0
11	17.0
12	18.0
13	22.0
14	22.0
15	35.0
16	55.0
17	112.0
18	225.0
19	424.0
20	741.0
21	1220.0
22	1845.0
23	2670.0
24	3485.0
25	4463.0
26	5430.0
27	6625.0
28	7954.0
29	9675.0
30	12501.0
31	16652.0
32	22762.0
33	32484.0
34	49687.0
35	64645.0
36	82199.0
37	124387.0
38	182408.0
39	182134.0
40	75.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.428493778626844	20.626174061027246	23.393369185522197	11.551962974823715
2	31.743665438465396	27.375759650459564	23.61643311210987	17.26414179896517
3	31.08122201731752	27.021041382162004	25.35370601450529	16.544030586015193
4	28.12396857473439	24.2594903394179	29.05892298650451	18.557618099343202
5	23.072515407729693	36.97327527290975	21.18113453405038	18.773074785310172
6	23.640236413406903	35.85292504536737	24.826352463089545	15.680486078136177
7	73.78287217504506	6.6189128271573585	13.622114003089521	5.97610099470806
8	71.29137817433588	8.884398162974087	11.881405572671847	7.9428180900181955
9	64.76669697293173	8.372995277375944	14.883320879544254	11.976986870148083
10	34.58865073317849	26.49368783074626	21.50935015760485	17.408311278470403
11	26.815400490544324	24.278140348681553	26.946809437395476	21.959649723378647
12	23.399013267273038	20.685436919411103	33.17848917747324	22.737060635842617
13	24.146117914683572	21.891798044939158	34.353439761083564	19.608644279293706
14	19.2881585937893	28.400773975384443	30.181236372916754	22.129831057909506
15	16.72329153031915	24.800954095210752	36.52763820945187	21.948116165018227
16	18.46792627847654	25.93897275258186	30.320252560388607	25.272848408552996
17	18.448908176924785	25.779588791835224	29.807868095355534	25.96363493588446
18	18.823503428779663	25.161071050400423	31.35127905935243	24.66414646146749
19	20.12434157491966	26.3075558303978	28.96420057103384	24.6039020236487
20	21.889098701493104	26.26252587382042	30.166758076251543	21.681617348434933
21	21.188864472100445	28.29218675039539	29.88651714757924	20.632431629924923
22	18.317744624932363	24.685250419318464	28.334517363526718	28.66248759222246
23	19.438462944762843	26.831105761503192	28.906655476661108	24.823775817072857
24	21.351438566010604	24.572614179160333	28.050840906832157	26.0251063479969
25	19.741157503009156	27.945443815006634	25.74511081418333	26.568287867800883
26	18.090018196028776	25.844250337111184	28.878066975618793	27.187664491241243
27	21.93584642208161	24.817763643033917	28.120287651853403	25.12610228303107
28	17.703643990954745	25.110519709501567	29.36026787302779	27.825568426515897
29	19.349752703331113	24.315808459496964	26.629881977342695	29.70455685982923
30	20.122746508337904	25.400208340235064	28.169366623599867	26.30767852782716
31	20.78138630917544	27.02619467419538	23.36060897188143	28.83181004474775
32	20.665314540995052	29.253521109479237	23.10785226738715	26.973312082138566
33	19.748396651341757	26.151852792532143	24.501204275269227	29.59854628085687
34	19.142394047702307	26.761659016481946	24.763040589536608	29.33290634627914
35	21.306408609433227	25.262909916774333	25.216284893615192	28.214396580177247
36	19.380304363243287	27.149014800990905	25.261314850192573	28.209365985573235
37	19.02705846409812	28.575985904519314	28.481999673624834	23.91495595775773
38	20.259554142081168	27.660294989159684	23.969679011255035	28.110471857504116
39	21.338555335927158	28.311818339093982	24.66426915889685	25.685357166082014
40	22.14737679030887	23.958513545182715	28.52727502506095	25.366834639447468
41	20.209861683187878	27.691828228506782	25.01052130456815	27.087788783737192
42	22.148112974885063	24.904633423025153	27.80630493010541	25.140948671984376
43	21.674868989819796	24.140596530362092	27.91710070882305	26.267433770995062
44	19.869253619267425	23.354106008125026	27.606676212526672	29.16996416008088
45	20.678075073649133	21.62419495149157	27.300300731399375	30.39742924345992
46	23.9698017086844	23.61250679437015	26.849510375908114	25.56818112103733
47	18.481913785424283	24.051518196642263	31.910533942403376	25.556034075530082
48	19.20018453693377	24.021948116165017	28.651444823579503	28.126422523321715
49	21.42616130049459	21.684439389310352	31.7759348623887	25.113464447806354
50	20.574518443264093	20.46458154655202	30.582579664373448	28.378320345810437
51	20.166917582909722	20.011582637332165	27.012452562106372	32.809047217651745
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	755.0
1	1153.0
2	1551.0
3	3009.0
4	4467.0
5	3054.5
6	1642.0
7	1651.5
8	1661.0
9	1647.5
10	1634.0
11	1678.5
12	1723.0
13	1756.5
14	1790.0
15	1904.5
16	2019.0
17	1915.5
18	1812.0
19	1901.0
20	1990.0
21	2079.5
22	2169.0
23	2774.0
24	3379.0
25	4118.0
26	5351.0
27	5845.0
28	6971.0
29	8097.0
30	9261.5
31	10426.0
32	11680.0
33	12934.0
34	13393.0
35	13852.0
36	16217.0
37	18582.0
38	19768.5
39	20955.0
40	23989.5
41	27024.0
42	31854.0
43	36684.0
44	46092.0
45	55500.0
46	79566.5
47	103633.0
48	105651.0
49	107669.0
50	101935.0
51	96201.0
52	78823.5
53	61446.0
54	52224.0
55	43002.0
56	38237.0
57	33472.0
58	31553.5
59	29635.0
60	27659.5
61	25684.0
62	22973.5
63	20263.0
64	17710.5
65	15158.0
66	13150.0
67	11142.0
68	9819.5
69	8497.0
70	7608.0
71	6719.0
72	5565.5
73	4412.0
74	3750.5
75	2399.0
76	1709.0
77	1288.0
78	867.0
79	656.5
80	446.0
81	389.0
82	332.0
83	258.5
84	185.0
85	125.0
86	65.0
87	47.0
88	29.0
89	16.0
90	3.0
91	3.5
92	4.0
93	3.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	815013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.951395961916454
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.46753948229951	23.924713354255324
2	12.194460561407137	8.280379181246154
3	4.793888441800643	4.882776140544849
4	2.6065929630219284	3.5398987919641023
5	1.5537476689252583	2.637595116629306
6	1.0635867979945741	2.166615391114838
7	0.7592153965188624	1.8043495783316714
8	0.6330409779629265	1.7194099922350516
9	0.5264130086529103	1.608521084665084
>10	5.209033472559489	35.39296086990152
>50	0.11350515559962074	2.5808287184672865
>100	0.0679561560584805	4.579866986275341
>500	0.005509958599336203	1.3280778677353662
>1k	0.0036733057328908014	2.041066990642972
>5k	0.0018366528664454007	3.512939935991154
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGC	6380	0.782809599356084	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTC	6036	0.7406016836541258	No Hit
GCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	5569	0.6833019841401303	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCG	5113	0.6273519563491625	No Hit
CTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGCT	5070	0.6220759668864178	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	3256	0.3995028300162083	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2305	0.2828175746889927	No Hit
TCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1636	0.2007329944430334	Illumina Single End Adapter 2 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCT	1412	0.17324877026501417	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCT	1333	0.16355567334508775	No Hit
ACTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTGC	1326	0.16269679133952464	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCC	1325	0.16257409391015848	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCACAGATCGTA	1314	0.16122442218713076	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCACAGAT	1257	0.15423066871325977	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTC	1202	0.14748231009812113	No Hit
AGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	905	0.1110411735763724	No Hit
CGCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	867	0.10637867126045843	No Hit
GCCTGTCTCTTATACACATCTGACGCCTCACAGATCGTATGCCGTCTTCTG	842	0.1033112355263045	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6552042728152803	0.0
2	0.0	0.0	0.0	2.1792290429723207	0.0
3	0.0	0.0	0.0	2.991363327946916	0.0
4	0.0	0.0	0.0	4.1135540169297915	0.0
5	0.0	0.0	0.0	7.031666979545111	0.0
6	0.0	0.0	0.0	8.584893737891298	0.0
7	0.0	0.0	0.0	10.181187293945005	0.0
8	0.0	0.0	0.0	12.720165199818899	0.0
9	0.0	0.0	0.0	13.58285082569235	0.0
10	0.0	0.0	0.0	16.112503726934417	0.0
11	0.0	0.0	0.0	18.66780039091401	0.0
12	0.0	0.0	0.0	21.640759104456002	0.0
13	0.0	0.0	0.0	22.56552962958873	0.0
14	0.0	0.0	0.0	22.960983444435854	0.0
15	0.0	0.0	0.0	23.74195258235145	0.0
16	0.0	0.0	0.0	24.984754844401255	0.0
17	0.0	0.0	0.0	26.438596684960853	0.0
18	0.0	0.0	0.0	28.09206724309919	0.0
19	0.0	0.0	0.0	29.150332571382297	0.0
20	0.0	0.0	0.0	30.125163647696418	0.0
21	0.0	0.0	0.0	31.128092435335386	0.0
22	0.0	0.0	0.0	32.182799538166876	0.0
23	0.0	0.0	0.0	33.141311856375296	0.0
24	0.0	0.0	0.0	33.95761785394834	0.0
25	0.0	0.0	0.0	34.629018187439954	0.0
26	0.0	0.0	0.0	35.24876290316841	0.0
27	0.0	0.0	0.0	35.884458284714476	0.0
28	0.0	0.0	0.0	36.529846763180466	0.0
29	0.0	0.0	0.0	37.198179660937924	0.0
30	1.2269742936615736E-4	0.0	0.0	37.929701734818956	0.0
31	1.2269742936615736E-4	0.0	0.0	38.57079580325713	0.0
32	1.2269742936615736E-4	0.0	0.0	39.21520270228818	0.0
33	1.2269742936615736E-4	0.0	0.0	39.831757284853126	0.0
34	1.2269742936615736E-4	0.0	0.0	40.428434883860746	0.0
35	1.2269742936615736E-4	0.0	0.0	41.036032554081956	0.0
36	1.2269742936615736E-4	0.0	0.0	41.61565521040769	0.0
37	1.2269742936615736E-4	0.0	0.0	42.20018576390806	0.0
38	1.2269742936615736E-4	0.0	0.0	42.76901104644956	0.0
39	1.2269742936615736E-4	0.0	0.0	43.33943139557283	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCATCG	60	0.0	45.000004	43
TATCACG	30	2.164721E-6	45.000004	1
TATACGG	60	0.0	45.000004	2
TTAATCG	25	3.889804E-5	45.0	20
CTATACG	20	7.032078E-4	45.0	1
GTATACG	20	7.032078E-4	45.0	1
ATGCGAG	45	3.8380676E-10	45.0	1
CGGTCTA	35	1.2113378E-7	45.0	31
TTTGACG	45	3.8380676E-10	45.0	1
TATCCGT	20	7.032078E-4	45.0	26
TCATACG	65	0.0	44.999996	31
CGTAAGG	160	0.0	42.1875	2
ATAGCGG	75	0.0	42.000004	2
TCTACGG	50	1.0804797E-9	40.5	2
TGCGATG	50	1.0804797E-9	40.5	1
GATAGCG	50	1.0804797E-9	40.5	1
TATGGGT	145	0.0	40.344826	4
GCGTAAG	95	0.0	40.263157	1
TAATACG	210	0.0	39.642857	4
TCACGAC	40	3.456571E-7	39.375	25
>>END_MODULE