##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550040_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	579084
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.884020280304757	33.0	31.0	34.0	30.0	34.0
2	31.965735540957787	33.0	31.0	34.0	30.0	34.0
3	31.955970118324803	33.0	31.0	34.0	30.0	34.0
4	35.695386161593134	37.0	35.0	37.0	33.0	37.0
5	31.57629981142632	37.0	35.0	37.0	0.0	37.0
6	33.253738663130044	37.0	35.0	37.0	17.0	37.0
7	35.188079794986564	37.0	35.0	37.0	32.0	37.0
8	35.34162919369211	37.0	35.0	37.0	33.0	37.0
9	37.3772734180188	39.0	37.0	39.0	34.0	39.0
10	37.08051681621319	39.0	37.0	39.0	33.0	39.0
11	37.14598227545572	39.0	37.0	39.0	34.0	39.0
12	37.116625567275214	39.0	37.0	39.0	34.0	39.0
13	36.99259347521258	39.0	37.0	39.0	33.0	39.0
14	37.96090031843394	40.0	37.0	41.0	33.0	41.0
15	38.13433802349918	40.0	37.0	41.0	33.0	41.0
16	38.037868426687666	40.0	37.0	41.0	33.0	41.0
17	38.00742033970892	40.0	37.0	41.0	33.0	41.0
18	38.01862424104275	40.0	37.0	41.0	33.0	41.0
19	37.98161406635307	40.0	37.0	41.0	33.0	41.0
20	38.03775272672013	40.0	37.0	41.0	33.0	41.0
21	37.921847262227935	40.0	37.0	41.0	33.0	41.0
22	37.9227210560126	40.0	36.0	41.0	33.0	41.0
23	37.68384552154782	39.0	36.0	41.0	33.0	41.0
24	37.43364347832093	39.0	36.0	41.0	32.0	41.0
25	37.3005159873179	39.0	35.0	41.0	32.0	41.0
26	37.623398332538976	39.0	36.0	41.0	33.0	41.0
27	37.7447037044712	39.0	36.0	41.0	33.0	41.0
28	37.66355140186916	39.0	36.0	41.0	33.0	41.0
29	37.721109545420006	40.0	36.0	41.0	33.0	41.0
30	37.54887546539017	39.0	36.0	41.0	33.0	41.0
31	37.48435287453979	39.0	36.0	41.0	33.0	41.0
32	37.43893286638899	39.0	36.0	41.0	33.0	41.0
33	37.398551850854105	40.0	36.0	41.0	33.0	41.0
34	37.26907495285658	39.0	36.0	41.0	33.0	41.0
35	37.271848298347045	40.0	36.0	41.0	33.0	41.0
36	37.13985535777193	39.0	35.0	41.0	32.0	41.0
37	37.06059224568456	39.0	35.0	41.0	32.0	41.0
38	36.935658729994266	39.0	35.0	41.0	32.0	41.0
39	36.91754736791208	39.0	35.0	41.0	32.0	41.0
40	36.84832770375282	39.0	35.0	41.0	31.0	41.0
41	36.7369069081515	39.0	35.0	41.0	31.0	41.0
42	36.803840202803045	39.0	35.0	41.0	31.0	41.0
43	36.707724613354884	39.0	35.0	41.0	31.0	41.0
44	36.61684660601916	39.0	35.0	40.0	31.0	41.0
45	36.58970201214331	39.0	35.0	40.0	31.0	41.0
46	36.53665616732633	39.0	35.0	40.0	31.0	41.0
47	36.476295321576835	39.0	35.0	40.0	31.0	41.0
48	36.43749093395777	39.0	35.0	40.0	31.0	41.0
49	36.42781358144932	39.0	35.0	40.0	31.0	41.0
50	36.317658578030134	39.0	35.0	40.0	31.0	41.0
51	35.26757258014381	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	15.0
10	14.0
11	15.0
12	15.0
13	15.0
14	19.0
15	25.0
16	38.0
17	95.0
18	153.0
19	299.0
20	526.0
21	903.0
22	1349.0
23	1944.0
24	2495.0
25	3131.0
26	3813.0
27	4651.0
28	5566.0
29	6752.0
30	8628.0
31	11286.0
32	15739.0
33	22915.0
34	36665.0
35	44940.0
36	55247.0
37	85965.0
38	130489.0
39	135333.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.47106810065552	20.44608381512872	23.433733275310665	12.649114808905098
2	31.08167381588854	26.847918436703484	24.90778539900947	17.162622348398504
3	30.657901098977003	25.576254912931457	27.375993810915165	16.389850177176367
4	28.022014077405004	25.08012654468091	28.549053332504435	18.348806045409646
5	22.88061835588619	36.386085611068516	22.760773911902245	17.972522121143047
6	24.346036153649557	35.010292116515046	25.327931699028124	15.315740030807277
7	73.35896001270973	6.835105097015286	13.417742503678223	6.388192386596764
8	71.29691029280725	7.976562985680833	12.176471807198956	8.550054914312948
9	64.74414765388096	8.795269770879527	15.747974387135544	10.712608188103971
10	34.20298264155114	27.444550358842584	21.337146251666425	17.01532074793985
11	27.861415615005768	24.156771729144648	26.56868433595126	21.413128319898323
12	23.980458793542905	20.98434769394423	33.15460278646967	21.8805907260432
13	24.20495126786442	22.623142756491283	34.29157082564878	18.88033514999551
14	18.67483819273197	28.578928100241068	30.20097257047337	22.54526113655359
15	17.18904338576096	25.594732370433306	36.258988333298795	20.957235910506938
16	19.9895006596625	25.990875244351425	29.60088691796009	24.41873717802599
17	19.08410524207196	26.84083828943642	28.4547664932894	25.620289975202216
18	20.52741916544059	25.087724751504098	31.223104074711095	23.161752008344212
19	20.06082019188926	27.407422757320184	28.7374543244158	23.79430272637476
20	22.569437249172832	27.17757700091869	28.992857685586205	21.260128064322277
21	21.00507007618929	29.075574528047742	29.977861588301526	19.941493807461438
22	18.432904380020858	25.259029778063287	28.674251058568363	27.633814783347493
23	19.395804408341448	26.853099032264748	29.225293739768322	24.52580281962548
24	21.99231890364783	24.89379779099405	27.880065759026323	25.233817546331792
25	19.23434251334867	27.64262179580165	27.037873607283224	26.08516208356646
26	19.207748789467505	25.276471116452882	30.039856048518004	25.47592404556161
27	23.078344419807834	24.48280387646697	29.266047758183618	23.172803945541578
28	18.87135545102265	24.868930932299975	31.738746019575743	24.520967597101627
29	22.508996967624732	23.682229175732708	28.760421631404082	25.048352225238478
30	25.16318876017987	24.26573692244994	27.63295135075395	22.93812296661624
31	23.13015037542049	26.1561362427558	27.42400066311623	23.289712718707474
32	24.921945693543595	24.96822568055757	27.784570114180323	22.325258511718506
33	24.95043896913056	23.21390333699429	25.613037141416445	26.222620552458707
34	20.786621630022587	23.257938399265047	31.796250630305796	24.159189340406574
35	23.969752229382955	21.890606544128314	29.368105490740547	24.77153573574818
36	24.894833910106307	23.66979574638567	29.516097837274042	21.919272506233984
37	24.096849507152676	25.492156578320248	30.71281541192642	19.69817850260066
38	22.063811122393297	25.666742648734896	28.440951571792695	23.828494657079112
39	26.471807198955595	23.11236366399348	26.980368996553178	23.435460140497753
40	22.385698793266606	22.44872937259534	31.232429146721373	23.933142687416677
41	21.038225887781394	24.140884569423434	28.08452659717761	26.736362945617557
42	24.356570031290797	21.449219802308473	31.180795877627425	23.013414288773305
43	26.690601018159715	19.841162940091593	29.92208384275856	23.54615219899013
44	23.78618645999544	20.94497516767861	28.971099184228887	26.297739188097065
45	22.861622838828218	19.49855288697322	29.327696845362677	28.312127428835886
46	25.16923278833468	21.17758390837944	29.68550331212743	23.967679991158448
47	19.99260901699926	22.15671646945866	34.02459746772489	23.826077045817186
48	20.77833267712456	21.558012309095055	31.752388254553743	25.911266759226642
49	24.064039068597992	18.947682892291965	33.38928376539501	23.59899427371504
50	22.13323110291426	18.451727210560126	32.17426141975948	27.240780266766134
51	21.17775659489815	18.79381920412237	28.790296399140715	31.238127801838765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	633.0
1	929.5
2	1226.0
3	2333.5
4	3441.0
5	2378.0
6	1315.0
7	1336.0
8	1357.0
9	1371.0
10	1385.0
11	1460.0
12	1535.0
13	1536.0
14	1537.0
15	1560.0
16	1583.0
17	1580.0
18	1577.0
19	1628.5
20	1680.0
21	1609.0
22	1538.0
23	1877.0
24	2216.0
25	2708.0
26	3654.5
27	4109.0
28	4887.5
29	5666.0
30	6460.5
31	7255.0
32	8199.0
33	9143.0
34	9430.5
35	9718.0
36	11052.5
37	12387.0
38	13322.5
39	14258.0
40	17337.5
41	20417.0
42	24288.0
43	28159.0
44	34007.5
45	39856.0
46	54716.5
47	69577.0
48	74718.0
49	79859.0
50	76309.0
51	72759.0
52	57776.5
53	42794.0
54	36574.0
55	30354.0
56	26580.5
57	22807.0
58	21131.5
59	19456.0
60	18368.5
61	17281.0
62	15287.0
63	13293.0
64	12310.0
65	11327.0
66	9768.0
67	8209.0
68	6851.5
69	5494.0
70	4496.5
71	3499.0
72	3146.0
73	2793.0
74	2330.5
75	1523.5
76	1179.0
77	962.5
78	746.0
79	533.0
80	320.0
81	242.0
82	164.0
83	117.0
84	70.0
85	46.0
86	22.0
87	18.5
88	15.0
89	9.5
90	4.0
91	2.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	579084.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.15685219035033
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.88580466970852	24.21235952497279
2	12.055000924248192	8.235217694481642
3	4.873337881601309	4.993736450864727
4	2.557621690550239	3.494412241718338
5	1.5413768814081206	2.632429115394016
6	1.0778399658748055	2.208937223954278
7	0.770096954469746	1.8412861492243442
8	0.6142195099519179	1.678384401108566
9	0.4645142863416272	1.4279711236989325
>10	4.917453314512646	34.57942129749159
>50	0.15530856831047604	3.4646654679959727
>100	0.07559721702523856	4.924026688237775
>500	0.006171201389815393	1.3938410306494988
>1k	0.005656934607330776	4.913311590207577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGCT	4887	0.8439190169301863	TruSeq Adapter, Index 3 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	4180	0.7218296482030241	RNA PCR Primer, Index 24 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	4157	0.7178578582727204	RNA PCR Primer, Index 3 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGC	3155	0.5448259665264452	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCG	2849	0.4919838918015349	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTC	2362	0.40788555719032127	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1750	0.3022014077405005	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1388	0.23968888796789412	RNA PCR Primer, Index 24 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTGC	1154	0.1992802425900215	TruSeq Adapter, Index 3 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCC	1058	0.1827023367939712	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	1031	0.178039800788832	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGCATGT	913	0.15766279158118685	No Hit
AGCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	721	0.12450697998908622	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTA	708	0.12226205524587107	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCT	701	0.12105324961490906	TruSeq Adapter, Index 3 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	689	0.11898101139040278	RNA PCR Primer, Index 24 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCT	683	0.11794489227814962	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	661	0.11414578886655477	No Hit
ACCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTCTTCTG	625	0.1079290741930359	TruSeq Adapter, Index 3 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGTGCATGTCGTATGCCGTC	611	0.10551146293111188	No Hit
GTGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	587	0.10136698648209931	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8743118442229452	0.0
2	0.0	0.0	0.0	2.832058906825262	0.0
3	0.0	0.0	0.0	3.850218621132685	0.0
4	0.0	0.0	0.0	5.0662770858804596	0.0
5	0.0	0.0	0.0	7.805257959121647	0.0
6	0.0	0.0	0.0	9.377914085003212	0.0
7	0.0	0.0	0.0	11.005657210352902	0.0
8	0.0	0.0	0.0	13.735313011583811	0.0
9	0.0	0.0	0.0	14.688024535300578	0.0
10	0.0	0.0	0.0	17.105463110705873	0.0
11	0.0	0.0	0.0	19.674347763018837	0.0
12	0.0	0.0	0.0	22.330266420761063	0.0
13	0.0	0.0	0.0	23.200433788534998	0.0
14	0.0	0.0	0.0	23.57792651843256	0.0
15	0.0	0.0	0.0	24.317715564581306	0.0
16	1.7268651870885743E-4	0.0	0.0	25.512015527971762	0.0
17	1.7268651870885743E-4	0.0	0.0	27.04650793321867	0.0
18	1.7268651870885743E-4	0.0	0.0	28.836921759192105	0.0
19	1.7268651870885743E-4	0.0	0.0	29.940388613741703	0.0
20	1.7268651870885743E-4	0.0	0.0	30.922629532157682	0.0
21	1.7268651870885743E-4	0.0	0.0	31.90884224050397	0.0
22	1.7268651870885743E-4	0.0	0.0	32.91905837495079	0.0
23	1.7268651870885743E-4	0.0	0.0	33.85398318724054	0.0
24	1.7268651870885743E-4	0.0	0.0	34.61466730215306	0.0
25	1.7268651870885743E-4	0.0	0.0	35.26932189457833	0.0
26	1.7268651870885743E-4	0.0	0.0	35.866817249310984	0.0
27	1.7268651870885743E-4	0.0	0.0	36.53459601715813	0.0
28	1.7268651870885743E-4	0.0	0.0	37.150914202430044	0.0
29	1.7268651870885743E-4	0.0	0.0	37.82577311754426	0.0
30	1.7268651870885743E-4	0.0	0.0	38.618922297974045	0.0
31	3.4537303741771487E-4	0.0	0.0	39.225570038198256	0.0
32	3.4537303741771487E-4	0.0	0.0	39.81995703559414	0.0
33	3.4537303741771487E-4	0.0	0.0	40.42798626796803	0.0
34	3.4537303741771487E-4	0.0	0.0	41.029453412630986	0.0
35	3.4537303741771487E-4	0.0	0.0	41.60968011549274	0.0
36	5.180595561265723E-4	0.0	0.0	42.156578320243696	0.0
37	5.180595561265723E-4	0.0	0.0	42.70589413625657	0.0
38	5.180595561265723E-4	0.0	0.0	43.28922919645509	0.0
39	5.180595561265723E-4	0.0	0.0	43.94422916191779	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGACG	20	7.0303824E-4	45.000004	1
GTTTGCG	20	7.0303824E-4	45.000004	1
TAATACG	80	0.0	45.000004	4
CTCAACG	20	7.0303824E-4	45.000004	1
GGCACGC	20	7.0303824E-4	45.000004	8
ACCGAAA	20	7.0303824E-4	45.000004	22
TCGATTA	40	6.8048394E-9	45.000004	41
ATACTCG	20	7.0303824E-4	45.000004	10
TGACCGA	20	7.0303824E-4	45.000004	33
AACGATC	20	7.0303824E-4	45.000004	42
TTGCGGA	20	7.0303824E-4	45.000004	1
TTGCGAC	20	7.0303824E-4	45.000004	1
TTCGGAC	20	7.0303824E-4	45.000004	13
GTCCATT	20	7.0303824E-4	45.000004	12
TACACGG	20	7.0303824E-4	45.000004	2
CGACGGT	50	2.1827873E-11	45.0	28
TTAACGG	25	3.8883994E-5	45.0	2
TAGCACG	30	2.1636297E-6	44.999996	1
GTACGAG	30	2.1636297E-6	44.999996	1
GCACGGG	235	0.0	44.042553	3
>>END_MODULE