SRR3550037_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3550037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222268
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19564	1.6006309581859297	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	12502	1.0228525986117611	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	7241	0.5924232656013247	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	6781	0.5547883115650578	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	4437	0.36301367621503633	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	4232	0.34624157713365644	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	3900	0.31907895813356807	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3894	0.31858806742874723	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	3508	0.287007432085271	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	3131	0.2561631327990261	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	2911	0.23816380695559403	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	2635	0.2155828345338338	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2517	0.20592865067235663	TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	2237	0.18302041778071584	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	2017	0.16502109193728381	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	1958	0.1601940000065452	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	1756	0.1436673462775758	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT	1420	0.11617746680760684	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	1332	0.10897773647023402	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	1296	0.10603239224130877	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	1278	0.10455972012684617	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	155	0.0	45.000004	31
CGTTTTT	7945	0.0	43.753933	1
CGTTATT	1255	0.0	43.745018	1
CGTAAGG	190	0.0	40.26316	2
TAACGCC	45	1.9284926E-8	40.0	12
GCGTTAG	75	0.0	39.0	1
TCTACGG	75	0.0	39.0	2
CGTTCTG	370	0.0	38.91892	1
ATATGCG	35	6.248614E-6	38.57143	1
ATGGGAC	605	0.0	38.305786	5
TATAGCG	65	9.094947E-12	38.07692	1
TATGGGA	610	0.0	37.991802	4
GTTTTTT	9280	0.0	37.50808	2
TCACGAC	180	0.0	37.5	25
TTAACGG	90	0.0	37.5	2
CGCATCG	30	1.13996444E-4	37.499996	21
TCGATAG	30	1.13996444E-4	37.499996	1
CGTTTCT	610	0.0	37.2541	1
GCGTAAG	115	0.0	37.173916	1
TAAACGG	55	2.750312E-9	36.818184	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content