##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550037_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1222268
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.923772855053066	33.0	31.0	34.0	30.0	34.0
2	32.04320738168716	33.0	31.0	34.0	30.0	34.0
3	31.960308213910533	33.0	31.0	34.0	30.0	34.0
4	35.7114380806828	37.0	35.0	37.0	33.0	37.0
5	31.717890020846493	37.0	35.0	37.0	0.0	37.0
6	33.331307863741834	37.0	35.0	37.0	17.0	37.0
7	35.2027321340328	37.0	35.0	37.0	32.0	37.0
8	35.36232642922829	37.0	35.0	37.0	33.0	37.0
9	37.41098187958778	39.0	37.0	39.0	34.0	39.0
10	37.12217124231347	39.0	37.0	39.0	34.0	39.0
11	37.16185484689119	39.0	37.0	39.0	34.0	39.0
12	37.11146982494837	39.0	37.0	39.0	34.0	39.0
13	37.00255426796742	39.0	37.0	39.0	33.0	39.0
14	37.97060710089767	40.0	37.0	41.0	33.0	41.0
15	38.13726694963788	40.0	37.0	41.0	33.0	41.0
16	38.07747809809305	40.0	37.0	41.0	33.0	41.0
17	38.05852480797992	40.0	37.0	41.0	33.0	41.0
18	37.954639244421024	39.0	37.0	41.0	33.0	41.0
19	37.842062460933285	39.0	37.0	41.0	33.0	41.0
20	37.737780912205835	39.0	36.0	41.0	33.0	41.0
21	37.62607464156797	39.0	35.0	41.0	33.0	41.0
22	37.654990558535445	39.0	35.0	41.0	33.0	41.0
23	37.44047786573812	39.0	35.0	41.0	33.0	41.0
24	37.209342795524385	39.0	35.0	41.0	32.0	41.0
25	37.045937552157135	39.0	35.0	41.0	32.0	41.0
26	37.33113687014632	39.0	35.0	41.0	33.0	41.0
27	37.44636937234714	39.0	35.0	41.0	33.0	41.0
28	37.3217944018824	39.0	35.0	41.0	33.0	41.0
29	37.28528440571135	39.0	35.0	41.0	33.0	41.0
30	37.07325398357807	39.0	35.0	41.0	32.0	41.0
31	36.91986618319387	39.0	35.0	41.0	31.0	41.0
32	36.67732854005832	39.0	35.0	41.0	31.0	41.0
33	36.4807513573128	39.0	35.0	41.0	30.0	41.0
34	36.25301652338113	39.0	35.0	41.0	30.0	41.0
35	36.14568490707439	39.0	35.0	41.0	30.0	41.0
36	35.966494254942454	39.0	35.0	41.0	29.0	41.0
37	35.9220997359008	39.0	35.0	41.0	29.0	41.0
38	35.75146530875389	39.0	35.0	40.0	28.0	41.0
39	35.707920848782756	39.0	35.0	40.0	27.0	41.0
40	35.660616984163866	39.0	35.0	40.0	27.0	41.0
41	35.49405040465757	39.0	35.0	40.0	26.0	41.0
42	35.53020695952115	39.0	35.0	40.0	27.0	41.0
43	35.457249146668325	38.0	35.0	40.0	26.0	41.0
44	35.411336139046426	38.0	35.0	40.0	26.0	41.0
45	35.334289206622444	38.0	35.0	40.0	26.0	41.0
46	35.25612140708912	38.0	35.0	40.0	26.0	41.0
47	35.15763564128326	38.0	34.0	40.0	26.0	41.0
48	35.08255308982972	38.0	34.0	40.0	25.0	41.0
49	35.04551620430217	38.0	34.0	40.0	26.0	41.0
50	34.9405204095992	38.0	34.0	40.0	24.0	41.0
51	33.86256696567365	36.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	19.0
10	28.0
11	25.0
12	20.0
13	21.0
14	43.0
15	65.0
16	124.0
17	224.0
18	457.0
19	913.0
20	1639.0
21	2675.0
22	4078.0
23	6053.0
24	8661.0
25	12856.0
26	17529.0
27	19706.0
28	19523.0
29	20428.0
30	22868.0
31	28169.0
32	36622.0
33	51344.0
34	79532.0
35	92548.0
36	113052.0
37	167256.0
38	247887.0
39	267795.0
40	103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.63642343577677	18.504370563575257	22.187768967198686	18.671437033449294
2	36.387682570434634	25.704182716065542	22.204704696515005	15.703430016984818
3	28.02339585099176	25.762680525056698	31.112243795959643	15.101679827991896
4	25.552170227806016	22.60821685587776	34.68044651418511	17.15916640213112
5	23.06171805201478	33.91498427513442	26.34847676614294	16.674820906707858
6	23.869069631210177	32.2471831055055	29.309774942974865	14.573972320309458
7	70.93853393854704	5.012975877630765	18.538242022207896	5.51024816161431
8	70.50941364741612	6.8443254670825056	16.464310609457172	6.181950276044206
9	64.01108431211485	6.618843003334785	18.69336348493129	10.676709199619069
10	35.467589759365374	23.451567086760022	24.80896170070721	16.271881453167392
11	27.647046310629094	21.154362218433274	31.36128901353877	19.837302457398867
12	23.70773021955905	18.504697824045135	35.94858083497236	21.83899112142345
13	23.486665772154716	19.398118906819125	38.92084223754529	18.194373083480873
14	18.30016003036977	24.958683365677576	36.216852605156966	20.52430399879568
15	17.05845199252537	22.278992823177894	40.40341398122179	20.25914120307494
16	20.44428881390988	23.683676575022826	33.65890295745287	22.21313165361443
17	20.368200754662645	23.94499406022247	32.92313960604385	22.763665579071038
18	21.19117902129484	22.868307114315357	33.90091207492956	22.03960178946025
19	20.915298445185506	25.22433705210314	31.275874030899935	22.58449047181142
20	21.45462369954871	25.489254402471474	32.073898686703735	20.98222321127609
21	20.413853590210984	26.19180081618761	33.68369293804632	19.710652655555084
22	19.132628850628503	22.28750159539479	33.90361197380607	24.67625758017063
23	19.250933510490334	24.582906531137198	33.26365412495459	22.902505833417873
24	20.47889660859975	23.577398737429107	32.508664220940084	23.435040433031055
25	21.37739022865689	26.098777027624052	29.681297391406797	22.84253535231226
26	20.36672808254818	23.328598965202392	31.09465354570356	25.210019406545864
27	22.976875775198238	22.015548144924026	31.114534619248808	23.89304146062893
28	20.846001040688293	22.857998409514117	31.935549323061718	24.360451226735872
29	24.34752443817559	22.282674503464055	29.94498751501307	23.424813543347287
30	26.617403057267307	22.598971747603635	30.017393893974152	20.766231301154903
31	25.106114207358775	22.706804072429286	30.244676290306217	21.94240542990572
32	25.66262063639071	24.821561228797613	28.97654196951896	20.53927616529272
33	26.018270952033433	23.499347115362585	28.07952102157628	22.402860911027698
34	23.805744730288282	22.62376172819709	30.63125272035266	22.93924082116197
35	23.44019478543167	24.569161591402214	29.733822696822624	22.256820926343487
36	24.187657698638922	26.12258522680787	29.419079939914976	20.27067713463823
37	21.940932757791252	27.23993428609765	29.853190953211573	20.965942002899528
38	22.072082391095897	25.734781569999377	30.290820016559376	21.902316022345346
39	23.3537980213832	25.09515098161778	28.103983741699857	23.447067255299164
40	23.98868333295153	22.16846059947573	32.12536039559246	21.717495671980284
41	20.752404546302447	25.061443153220083	29.683179139108606	24.502973161368864
42	21.280602944689708	24.64598598670668	32.341925011535935	21.73148605706768
43	22.836399218502	23.400023562753834	31.148569708116387	22.615007510627784
44	23.223139278783375	22.417342186819912	29.985158737690913	24.374359796705797
45	21.93471480886352	21.439978793521554	29.850081978747706	26.77522441886722
46	24.172358271672007	22.577208926356576	29.47070527903864	23.779727522932777
47	20.841828469697315	22.620898199085634	33.38956759074115	23.147705740475903
48	20.570202279696435	22.898251447309427	31.761774013555126	24.76977225943901
49	21.734840476883956	21.238877234779935	33.36085048450912	23.665431803826984
50	20.47996020512686	21.23642278125583	33.49510909227764	24.788507921339672
51	20.90629878226379	20.666662303193732	30.95311339248021	27.473925522062263
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	729.0
1	1146.0
2	1563.0
3	17078.5
4	32594.0
5	20667.0
6	8740.0
7	8219.5
8	7699.0
9	7549.5
10	7400.0
11	7207.5
12	7015.0
13	6829.5
14	6644.0
15	6184.0
16	5724.0
17	5375.5
18	5027.0
19	4862.5
20	4698.0
21	4866.5
22	5035.0
23	5312.5
24	5590.0
25	6409.0
26	8100.0
27	8972.0
28	10781.5
29	12591.0
30	14317.5
31	16044.0
32	17567.0
33	19090.0
34	21174.0
35	23258.0
36	25605.5
37	27953.0
38	30703.0
39	33453.0
40	36587.5
41	39722.0
42	45032.0
43	50342.0
44	60168.5
45	69995.0
46	104811.5
47	139628.0
48	134115.5
49	128603.0
50	126081.5
51	123560.0
52	105282.0
53	87004.0
54	76856.5
55	66709.0
56	61415.5
57	56122.0
58	52708.0
59	49294.0
60	45523.0
61	41752.0
62	38090.5
63	34429.0
64	30910.0
65	27391.0
66	23595.0
67	19799.0
68	17159.5
69	14520.0
70	12354.0
71	10188.0
72	8372.0
73	6556.0
74	5405.5
75	3395.0
76	2535.0
77	1996.0
78	1457.0
79	1074.5
80	692.0
81	524.5
82	357.0
83	264.5
84	172.0
85	134.0
86	96.0
87	60.5
88	25.0
89	15.5
90	6.0
91	7.5
92	9.0
93	5.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1222268.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.2503816827354
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.29972206257922	28.419927278148116
2	8.961137303024334	6.855338443041578
3	3.6650291519562583	4.2056629182203675
4	2.1745009250942817	3.3270196141727006
5	1.5914455033122263	3.04366989644828
6	1.239876460833965	2.845544871980298
7	1.0799241017700885	2.8915256356763757
8	0.941617628788729	2.881378696028892
9	0.8243553068608503	2.8378714616654395
>10	5.12213179819846	30.23099322368217
>50	0.05704146384262807	1.4898302321260488
>100	0.03542117928424113	2.491604014265654
>500	0.0031188457818790427	0.7639271268880986
>1k	0.00378716987799598	3.7884479792881183
>5k	4.455493974112918E-4	1.1948450834606714
>10k+	4.455493974112918E-4	2.7324135249072805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19564	1.6006309581859297	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	12502	1.0228525986117611	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	7241	0.5924232656013247	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	6781	0.5547883115650578	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	4437	0.36301367621503633	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTG	4232	0.34624157713365644	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	3900	0.31907895813356807	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	3894	0.31858806742874723	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	3508	0.287007432085271	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	3131	0.2561631327990261	No Hit
CTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGCT	2911	0.23816380695559403	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	2635	0.2155828345338338	No Hit
CCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	2517	0.20592865067235663	TruSeq Adapter, Index 13 (95% over 21bp)
CGTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	2237	0.18302041778071584	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCC	2017	0.16502109193728381	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	1958	0.1601940000065452	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	1756	0.1436673462775758	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTAGCTAT	1420	0.11617746680760684	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	1332	0.10897773647023402	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	1296	0.10603239224130877	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	1278	0.10455972012684617	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.45445352410437E-4	0.0	0.0	0.2529723432176904	0.0
2	2.45445352410437E-4	0.0	0.0	0.9174747273102135	0.0
3	3.2726046988058264E-4	0.0	0.0	1.6194484352040632	0.0
4	3.2726046988058264E-4	0.0	0.0	2.5906756946921625	0.0
5	3.2726046988058264E-4	0.0	0.0	5.105345145254559	0.0
6	3.2726046988058264E-4	0.0	0.0	7.225747544728325	0.0
7	3.2726046988058264E-4	0.0	0.0	8.842577896173344	0.0
8	3.2726046988058264E-4	0.0	0.0	10.966498345698325	0.0
9	3.2726046988058264E-4	0.0	0.0	12.033776553096375	0.0
10	3.2726046988058264E-4	0.0	0.0	14.162933170139446	0.0
11	4.0907558735072834E-4	0.0	0.0	15.848242774906977	0.0
12	4.0907558735072834E-4	0.0	0.0	17.78177944607893	0.0
13	4.0907558735072834E-4	0.0	0.0	18.461335811785958	0.0
14	4.0907558735072834E-4	0.0	0.0	18.857648240811343	0.0
15	4.0907558735072834E-4	0.0	0.0	19.374228892517845	0.0
16	4.0907558735072834E-4	0.0	0.0	20.04036757895977	0.0
17	4.0907558735072834E-4	0.0	0.0	20.794212071329692	0.0
18	4.0907558735072834E-4	0.0	0.0	21.617190337961887	0.0
19	4.0907558735072834E-4	0.0	0.0	22.272365798662815	0.0
20	4.0907558735072834E-4	0.0	0.0	22.84417165466166	0.0
21	4.0907558735072834E-4	0.0	0.0	23.44420372618771	0.0
22	4.0907558735072834E-4	0.0	0.0	24.1181148487893	0.0
23	4.0907558735072834E-4	0.0	0.0	24.71331982838461	0.0
24	4.90890704820874E-4	0.0	0.0	25.217791842705527	0.0
25	4.90890704820874E-4	0.0	0.0	25.666220501559398	0.0
26	4.90890704820874E-4	0.0	0.0	26.13444841884104	0.0
27	5.727058222910196E-4	0.0	0.0	26.63482967728845	0.0
28	5.727058222910196E-4	0.0	0.0	27.07753127791941	0.0
29	5.727058222910196E-4	0.0	0.0	27.55017721154444	0.0
30	5.727058222910196E-4	0.0	0.0	28.11061076621494	0.0
31	6.545209397611653E-4	0.0	0.0	28.564275592586895	0.0
32	6.545209397611653E-4	0.0	0.0	29.0315217284589	0.0
33	6.545209397611653E-4	0.0	0.0	29.514476366885166	0.0
34	6.545209397611653E-4	0.0	0.0	29.99718555995903	0.0
35	6.545209397611653E-4	0.0	0.0	30.46475895630091	0.0
36	6.545209397611653E-4	0.0	0.0	30.915396623326473	0.0
37	6.545209397611653E-4	0.0	0.0	31.373070390454465	0.0
38	6.545209397611653E-4	0.0	0.0	31.88007867341696	0.0
39	6.545209397611653E-4	0.0	0.0	32.5763253230879	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	155	0.0	45.000004	31
CGTTTTT	7945	0.0	43.753933	1
CGTTATT	1255	0.0	43.745018	1
CGTAAGG	190	0.0	40.26316	2
TAACGCC	45	1.9284926E-8	40.0	12
GCGTTAG	75	0.0	39.0	1
TCTACGG	75	0.0	39.0	2
CGTTCTG	370	0.0	38.91892	1
ATATGCG	35	6.248614E-6	38.57143	1
ATGGGAC	605	0.0	38.305786	5
TATAGCG	65	9.094947E-12	38.07692	1
TATGGGA	610	0.0	37.991802	4
GTTTTTT	9280	0.0	37.50808	2
TCACGAC	180	0.0	37.5	25
TTAACGG	90	0.0	37.5	2
CGCATCG	30	1.13996444E-4	37.499996	21
TCGATAG	30	1.13996444E-4	37.499996	1
CGTTTCT	610	0.0	37.2541	1
GCGTAAG	115	0.0	37.173916	1
TAAACGG	55	2.750312E-9	36.818184	2
>>END_MODULE