FastQCFastQC Report
Sat 18 Jun 2016
SRR3550034_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550034_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533373
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC400852.6141715029545978No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC259561.6927388182783967No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG249771.628892643864213No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224331.462983892373219No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT108420.7070686649627977Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC90310.5889630246521883Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT70060.45690122364225794No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC63850.4164022713325459No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT60370.39370720627009864No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT59870.3904464210599769No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG58730.38301183078089934Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT50730.3308392674189516No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC46480.3031225931329168No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA46400.3026008674992973No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT41350.2696669368770677Illumina PCR Primer Index 5 (95% over 24bp)
GAATATGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC38220.24925442146170565No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT36150.23575477069180165No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC35340.23047229865140445Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC26370.1719738119818205No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT25900.16890867388430603Illumina PCR Primer Index 5 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC25570.1667565556456257No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG24900.16238710346406257No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC18000.11738826756438259No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG302.165978E-644.9999961
TAATACG8300.043.644584
CGTTTTT96300.043.504671
CGTTATT13600.043.1801451
CTCGAAT1950.042.6923143
CGACGGT1400.041.78571328
CGATGAA19800.041.59090819
AATACGG8750.041.3999985
CCGATGA19600.040.98214318
TACGGCT34500.040.760877
GATGAAT21600.040.020
CGTTTCT7350.039.7959181
ATAATAC11000.039.681823
ACGGCTG35950.039.1794138
ACGGGTA1850.038.918925
CACCGAT22100.038.89140316
CGACGAA356.2496565E-638.57142619
GTTATTT15050.038.421932
ATGAATG22050.038.36734821
TACGGGA5000.038.2500044