##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550034_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1533373
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.783341039655713	31.0	31.0	34.0	30.0	34.0
2	31.903208808293872	33.0	31.0	34.0	30.0	34.0
3	31.842616245362347	33.0	31.0	34.0	30.0	34.0
4	35.626008805424384	37.0	35.0	37.0	33.0	37.0
5	31.740255632517332	37.0	35.0	37.0	0.0	37.0
6	33.261380629501105	36.0	35.0	37.0	17.0	37.0
7	35.09163849891709	37.0	35.0	37.0	32.0	37.0
8	35.122162057112	37.0	35.0	37.0	32.0	37.0
9	37.20223389873175	39.0	37.0	39.0	34.0	39.0
10	36.98590166906552	39.0	37.0	39.0	33.0	39.0
11	37.01529243047843	39.0	37.0	39.0	33.0	39.0
12	36.96307617259467	39.0	37.0	39.0	33.0	39.0
13	36.78658682525387	39.0	35.0	39.0	33.0	39.0
14	37.70948034170421	40.0	37.0	41.0	32.0	41.0
15	37.80968883631054	40.0	37.0	41.0	33.0	41.0
16	37.841468448968385	40.0	37.0	41.0	33.0	41.0
17	37.736814851963615	40.0	36.0	41.0	33.0	41.0
18	37.64801649696454	39.0	36.0	41.0	33.0	41.0
19	37.56872137438184	39.0	36.0	41.0	33.0	41.0
20	37.34783513209115	39.0	35.0	41.0	32.0	41.0
21	37.36912023362874	39.0	35.0	41.0	32.0	41.0
22	37.420805635680296	39.0	35.0	41.0	33.0	41.0
23	37.14030310954999	39.0	35.0	41.0	32.0	41.0
24	36.91034797143291	39.0	35.0	40.0	31.0	41.0
25	36.80329443651349	39.0	35.0	40.0	31.0	41.0
26	37.04779267666771	39.0	35.0	40.0	32.0	41.0
27	37.12597978443601	39.0	35.0	40.0	32.0	41.0
28	37.03142744785515	39.0	35.0	41.0	32.0	41.0
29	37.05420924980419	39.0	35.0	41.0	32.0	41.0
30	36.77681881707843	39.0	35.0	40.0	31.0	41.0
31	36.720781571085446	39.0	35.0	40.0	31.0	41.0
32	36.50871510063109	39.0	35.0	40.0	30.0	41.0
33	36.30916287165615	39.0	35.0	40.0	30.0	41.0
34	36.100325230716855	39.0	35.0	41.0	30.0	41.0
35	35.90525397277766	39.0	35.0	41.0	29.0	41.0
36	35.79477270044536	39.0	35.0	40.0	28.0	41.0
37	35.72736705289581	39.0	35.0	40.0	27.0	41.0
38	35.617379463444315	39.0	35.0	40.0	27.0	41.0
39	35.550702275310705	39.0	35.0	40.0	26.0	41.0
40	35.38801778823548	38.0	35.0	40.0	26.0	41.0
41	35.25450298133592	38.0	35.0	40.0	24.0	41.0
42	35.316136386906514	38.0	35.0	40.0	25.0	41.0
43	35.268117411745216	38.0	35.0	40.0	25.0	41.0
44	35.24034139116836	38.0	35.0	40.0	25.0	41.0
45	35.19467213782948	38.0	35.0	40.0	25.0	41.0
46	35.07911186645389	38.0	34.0	40.0	25.0	41.0
47	34.87948463941911	38.0	34.0	40.0	24.0	41.0
48	34.85877604470667	38.0	34.0	40.0	24.0	41.0
49	34.90752413144095	38.0	34.0	40.0	24.0	41.0
50	34.76661190721371	37.0	34.0	40.0	24.0	41.0
51	33.67204783180609	36.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	8.0
9	11.0
10	29.0
11	26.0
12	32.0
13	35.0
14	38.0
15	66.0
16	139.0
17	282.0
18	620.0
19	1295.0
20	2576.0
21	4906.0
22	7346.0
23	10055.0
24	13202.0
25	17272.0
26	21566.0
27	24047.0
28	24467.0
29	26453.0
30	30590.0
31	38528.0
32	51107.0
33	70074.0
34	105134.0
35	119376.0
36	145309.0
37	208104.0
38	304006.0
39	306593.0
40	79.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.35471799751267	17.278835612730887	20.298714011528833	17.067732378227607
2	33.454938883102805	31.443556134091317	20.036481664930843	15.065023317875037
3	25.58079475770083	31.697049576326176	28.631194106065514	14.090961559907472
4	23.531521684547727	20.064002692104268	39.62493144199096	16.779544181357046
5	26.305993388431908	31.059500852043175	25.038526177257587	17.59597958226733
6	21.982192199810484	34.58154017319986	29.150506758629508	14.285760868360144
7	66.20333082687644	4.585250946768986	23.51802203377782	5.693396192576757
8	63.87167375452678	11.112429917573872	17.830430038875082	7.185466289024261
9	58.27251425452255	5.85473984477358	18.753493116156342	17.11925278454753
10	36.71337632787326	20.82604819570972	25.452711114647254	17.007864361769773
11	29.590777977700146	19.12222270771691	30.57475252270648	20.71224679187647
12	23.33398331651855	16.684850978855113	35.51347258625266	24.467693118373678
13	23.28604977392976	18.3880895255101	40.78648834954052	17.539372351019615
14	18.12187902095576	26.33071014032463	34.87051095852085	20.67689988019875
15	15.581140400933105	21.16243079798588	42.0555859533199	21.200842847761113
16	18.780035907766734	24.033095665568652	31.663593920070326	25.523274506594284
17	18.916336729549823	25.108633059275203	33.07270964077234	22.902320570402633
18	19.77848833910601	23.288854049210467	32.48107277224785	24.451584839435675
19	18.88203326913934	25.589664093472365	29.66923246985567	25.85907016753262
20	21.02241268106325	26.050869553591983	33.151685858561486	19.77503190678328
21	19.15065675474917	28.98270675171664	32.79795587896748	19.06868061456671
22	17.855798947809827	20.663400229428845	34.832555418675035	26.648245404086286
23	19.50979963779198	25.91287312349963	33.105774002802974	21.471553235905418
24	21.971105530096068	23.13331459468766	30.106829845054005	24.788750030162262
25	20.468144411046758	29.439477543950492	27.28820710942478	22.80417093557797
26	20.17434766361479	21.523399720746355	31.720592445543254	26.5816601700956
27	24.21361273480099	21.59441962262281	29.481932967386275	24.710034675189924
28	18.99524773163477	22.878777701185555	32.833107143532594	25.292867423647085
29	25.75700759045581	19.89502880251576	30.73818307743778	23.609780529590648
30	23.513000424554235	21.86297789252843	34.9894644029861	19.634557279931236
31	23.382895094670378	19.295892128007992	33.39422306249034	23.92698971483129
32	25.806375878537057	20.70474698589319	33.70321506900147	19.78566206656828
33	25.401060276918923	18.237376033098272	30.639250854162686	25.722312835820116
34	24.115397884272124	18.693494668290104	32.27714326520684	24.913964182230938
35	21.102497565823843	19.380346464950147	35.44682213655777	24.070333832668243
36	23.1880305705135	21.157800482987504	31.022327900647788	24.631841045851203
37	18.918227984971693	20.51992568018349	37.30546970632716	23.256376628517653
38	22.101797801317748	20.43547134324134	32.27055647908239	25.192174376358523
39	23.553695023976555	19.30254412983664	30.31519401998079	26.828566826206018
40	23.917729084834544	18.647256734010575	34.47582551668772	22.95918866446716
41	19.32250013532259	22.182600058824566	28.990402204812526	29.50449760104032
42	20.347104064047038	20.411341532686436	36.15128217335247	23.090272229914053
43	23.60215029219896	19.040963940280676	30.679945453585006	26.676940313935354
44	23.5469125907395	18.820273997259637	31.077174307882032	26.55563910411883
45	21.319339782296936	17.390093604100244	31.177084766720164	30.113481846882657
46	26.265820514643206	20.044568412251945	29.39526129650124	24.294349776603603
47	19.090919169699742	19.676295330620793	37.71606777998569	23.516717719693773
48	20.621010021697263	21.07817210815633	31.177410845241177	27.123407024905227
49	21.051433669433333	17.6220006482441	36.61229198635949	24.714273695963083
50	20.71224679187647	17.553850237352556	34.598300609179894	27.135602361591083
51	20.376385915233932	17.64260881077207	31.00328491502068	30.977720358973322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	756.0
1	1237.5
2	1719.0
3	19431.5
4	37144.0
5	23460.5
6	9777.0
7	9062.0
8	8347.0
9	8267.0
10	8187.0
11	8038.5
12	7890.0
13	7452.5
14	7015.0
15	6600.0
16	6185.0
17	5902.0
18	5619.0
19	5342.0
20	5065.0
21	5181.5
22	5298.0
23	5704.5
24	6111.0
25	7167.0
26	8709.0
27	9195.0
28	10694.0
29	12193.0
30	14292.0
31	16391.0
32	17821.5
33	19252.0
34	21568.0
35	23884.0
36	25979.5
37	28075.0
38	30744.5
39	33414.0
40	37901.5
41	42389.0
42	48025.5
43	53662.0
44	71388.0
45	89114.0
46	169668.0
47	250222.0
48	206376.5
49	162531.0
50	158470.0
51	154409.0
52	130232.5
53	106056.0
54	94508.5
55	82961.0
56	76614.5
57	70268.0
58	66554.5
59	62841.0
60	56578.0
61	50315.0
62	46410.5
63	42506.0
64	37828.0
65	33150.0
66	28009.0
67	22868.0
68	19850.0
69	16832.0
70	14357.0
71	11882.0
72	10295.0
73	8708.0
74	7273.0
75	4619.5
76	3401.0
77	2731.0
78	2061.0
79	1496.5
80	932.0
81	650.5
82	369.0
83	269.5
84	170.0
85	108.0
86	46.0
87	56.5
88	67.0
89	44.0
90	21.0
91	14.5
92	8.0
93	6.5
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1533373.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.43379244760985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25091472827206	25.567405867984977
2	9.45544588497824	6.511737222058946
3	3.7018526510169436	3.8240647757025514
4	2.082813237896793	2.868766349634896
5	1.4956328106393328	2.575015488969507
6	1.2120362270812117	2.5041002329379136
7	0.9320697155330808	2.2466286591958196
8	0.8142528191397608	2.2430250059311776
9	0.719026536406485	2.2282929467050216
>10	5.206470372562013	29.388324348358257
>50	0.0754220257687579	1.7563597052607989
>100	0.04498672611647691	2.974948768297951
>500	0.003354271695894788	0.822905633973886
>1k	0.003354271695894788	2.9597969813770124
>5k	0.0013811706983096186	3.08399376959566
>10k+	9.865504987925848E-4	8.44463424401554
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	40085	2.6141715029545978	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	25956	1.6927388182783967	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	24977	1.628892643864213	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22433	1.462983892373219	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	10842	0.7070686649627977	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	9031	0.5889630246521883	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	7006	0.45690122364225794	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	6385	0.4164022713325459	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	6037	0.39370720627009864	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTT	5987	0.3904464210599769	No Hit
CGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTG	5873	0.38301183078089934	Illumina PCR Primer Index 5 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTTTGCTCT	5073	0.3308392674189516	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	4648	0.3031225931329168	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTA	4640	0.3026008674992973	No Hit
CTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGCT	4135	0.2696669368770677	Illumina PCR Primer Index 5 (95% over 24bp)
GAATATGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTC	3822	0.24925442146170565	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCT	3615	0.23575477069180165	No Hit
CCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCTGC	3534	0.23047229865140445	Illumina PCR Primer Index 5 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCC	2637	0.1719738119818205	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTCTTCT	2590	0.16890867388430603	Illumina PCR Primer Index 5 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGC	2557	0.1667565556456257	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCG	2490	0.16238710346406257	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTTGCTCTCGTATGCCGTC	1800	0.11738826756438259	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.285905647223474	0.0
2	0.0	0.0	0.0	1.2783582337761261	0.0
3	0.0	0.0	0.0	2.0465340135766055	0.0
4	0.0	0.0	0.0	3.4499759680130015	0.0
5	0.0	0.0	0.0	7.719126396512785	0.0
6	0.0	0.0	0.0	10.052348645763294	0.0
7	0.0	0.0	0.0	12.024210678028112	0.0
8	0.0	0.0	0.0	14.42975714323912	0.0
9	0.0	0.0	0.0	15.4925774746262	0.0
10	0.0	0.0	0.0	18.856729575908798	0.0
11	0.0	0.0	0.0	20.582989266147244	0.0
12	0.0	0.0	0.0	23.827927060147793	0.0
13	0.0	0.0	0.0	24.570016558267298	0.0
14	0.0	0.0	0.0	25.004940089593333	0.0
15	0.0	0.0	0.0	25.754725040808726	0.0
16	0.0	0.0	0.0	26.459772018941248	0.0
17	0.0	0.0	0.0	27.20199194846916	0.0
18	6.521570420243476E-5	0.0	0.0	27.999514795160735	0.0
19	6.521570420243476E-5	0.0	0.0	28.930142894129478	0.0
20	6.521570420243476E-5	0.0	0.0	29.532214275326357	0.0
21	6.521570420243476E-5	0.0	0.0	30.12195988842897	0.0
22	1.3043140840486952E-4	0.0	0.0	30.822441767267325	0.0
23	1.3043140840486952E-4	0.0	0.0	31.397252984107585	0.0
24	1.3043140840486952E-4	0.0	0.0	31.8744362917568	0.0
25	1.9564711260730428E-4	0.0	0.0	32.297816643439006	0.0
26	1.9564711260730428E-4	0.0	0.0	32.74330511884584	0.0
27	1.9564711260730428E-4	0.0	0.0	33.263530791268664	0.0
28	1.9564711260730428E-4	0.0	0.0	33.709084482379694	0.0
29	1.9564711260730428E-4	0.0	0.0	34.16911605982367	0.0
30	1.9564711260730428E-4	0.0	0.0	34.709167306324034	0.0
31	2.6086281680973904E-4	0.0	0.0	35.12563479336078	0.0
32	2.6086281680973904E-4	0.0	0.0	35.57744919207525	0.0
33	2.6086281680973904E-4	0.0	0.0	36.01426397882316	0.0
34	3.9129422521460856E-4	0.0	0.0	36.460078532751	0.0
35	3.9129422521460856E-4	0.0	0.0	36.88939351351563	0.0
36	3.9129422521460856E-4	0.0	0.0	37.305730569143975	0.0
37	3.9129422521460856E-4	0.0	0.0	37.742936650117095	0.0
38	3.9129422521460856E-4	0.0	0.0	38.239032511985016	0.0
39	3.9129422521460856E-4	0.0	0.0	38.95399227715631	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTACG	30	2.165978E-6	44.999996	1
TAATACG	830	0.0	43.64458	4
CGTTTTT	9630	0.0	43.50467	1
CGTTATT	1360	0.0	43.180145	1
CTCGAAT	195	0.0	42.69231	43
CGACGGT	140	0.0	41.785713	28
CGATGAA	1980	0.0	41.590908	19
AATACGG	875	0.0	41.399998	5
CCGATGA	1960	0.0	40.982143	18
TACGGCT	3450	0.0	40.76087	7
GATGAAT	2160	0.0	40.0	20
CGTTTCT	735	0.0	39.795918	1
ATAATAC	1100	0.0	39.68182	3
ACGGCTG	3595	0.0	39.179413	8
ACGGGTA	185	0.0	38.91892	5
CACCGAT	2210	0.0	38.891403	16
CGACGAA	35	6.2496565E-6	38.571426	19
GTTATTT	1505	0.0	38.42193	2
ATGAATG	2205	0.0	38.367348	21
TACGGGA	500	0.0	38.250004	4
>>END_MODULE