FastQCFastQC Report
Sat 18 Jun 2016
SRR3550033_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550033_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264257
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152951.209801488146793No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC58240.46066582981150195No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT52160.4125743420839275RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT48720.3853646845538526No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC41660.3295216083438731RNA PCR Primer, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG38970.30824428893808775RNA PCR Primer, Index 16 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC38220.30231195081379814No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG36990.2925829162899632No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC36400.2879161436321887No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC34550.2732830429256077RNA PCR Primer, Index 16 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT30440.24077383000450067No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC26410.20889739981665123No Hit
CGTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT23970.1895975264522957RNA PCR Primer, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT17160.1357318962837461RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC16870.13343805887568747No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC13060.10330178120429628RNA PCR Primer, Index 14 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG253.891027E-545.0000041
CGTTTTT70750.043.50531
CGTTATT9050.042.5138131
GCGTACG650.041.538461
GCGTAAG1050.040.7142871
CGAATAT950.040.26315714
CACGGGC2550.039.7058834
CATAAGG1700.039.7058832
CGTAAGG2100.039.6428572
ACGGGAG7400.039.2229735
TAATACG1100.038.8636364
TACGGGT1250.037.8000034
ACGACCA2800.037.7678628
TTAAGCG301.1399862E-437.5000041
CGGTCTA1200.037.50000431
CACAACG5650.037.43362812
TATGGGA9050.037.292824
ACGGGTA1750.037.2857135
AGCGACT5200.037.21153619
GTTTTTT83100.036.958482
Produced by FastQC (version 0.11.5)