##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550033_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1264257
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.895481693991016	33.0	31.0	34.0	30.0	34.0
2	32.0112738153714	33.0	31.0	34.0	30.0	34.0
3	31.94493445557351	33.0	31.0	34.0	30.0	34.0
4	35.70244420240505	37.0	35.0	37.0	33.0	37.0
5	31.58139207455446	37.0	35.0	37.0	0.0	37.0
6	33.270133366870816	37.0	35.0	37.0	17.0	37.0
7	35.2180877780388	37.0	35.0	37.0	32.0	37.0
8	35.42330554626156	37.0	35.0	37.0	33.0	37.0
9	37.451515000510184	39.0	37.0	39.0	35.0	39.0
10	37.10836878894086	39.0	37.0	39.0	34.0	39.0
11	37.17131168741798	39.0	37.0	39.0	34.0	39.0
12	37.155975406899074	39.0	37.0	39.0	34.0	39.0
13	37.063347879426416	39.0	37.0	39.0	33.0	39.0
14	38.00912077212149	40.0	37.0	41.0	33.0	41.0
15	38.17616987685257	40.0	37.0	41.0	33.0	41.0
16	38.09662750532526	40.0	37.0	41.0	33.0	41.0
17	38.097302209914595	40.0	37.0	41.0	33.0	41.0
18	38.03835217048432	40.0	37.0	41.0	33.0	41.0
19	37.94108950949055	40.0	37.0	41.0	33.0	41.0
20	37.91909951853144	40.0	36.0	41.0	33.0	41.0
21	37.78416176457793	39.0	36.0	41.0	33.0	41.0
22	37.822209408371876	39.0	36.0	41.0	33.0	41.0
23	37.58749210010306	39.0	35.0	41.0	33.0	41.0
24	37.353192428438206	39.0	35.0	41.0	32.0	41.0
25	37.19611677056168	39.0	35.0	41.0	32.0	41.0
26	37.510195316300404	39.0	35.0	41.0	33.0	41.0
27	37.643933946974386	39.0	36.0	41.0	33.0	41.0
28	37.55088799191937	39.0	35.0	41.0	33.0	41.0
29	37.56934230935641	39.0	36.0	41.0	33.0	41.0
30	37.347562244069046	39.0	35.0	41.0	33.0	41.0
31	37.228140322735015	39.0	35.0	41.0	32.0	41.0
32	37.06182840988818	39.0	35.0	41.0	32.0	41.0
33	36.894936709861994	39.0	35.0	41.0	31.0	41.0
34	36.64290409307601	39.0	35.0	41.0	31.0	41.0
35	36.54258509148061	39.0	35.0	41.0	30.0	41.0
36	36.41245490434302	39.0	35.0	41.0	30.0	41.0
37	36.38267693989434	39.0	35.0	41.0	30.0	41.0
38	36.24274257528335	39.0	35.0	41.0	30.0	41.0
39	36.188199867590214	39.0	35.0	41.0	30.0	41.0
40	36.085072101637564	39.0	35.0	40.0	30.0	41.0
41	35.954753661636836	39.0	35.0	40.0	29.0	41.0
42	36.03514554398354	39.0	35.0	40.0	30.0	41.0
43	35.993918957933396	39.0	35.0	40.0	29.0	41.0
44	35.964319754606855	39.0	35.0	40.0	29.0	41.0
45	35.8861592223733	39.0	35.0	40.0	29.0	41.0
46	35.79758071341507	38.0	35.0	40.0	29.0	41.0
47	35.73144226213499	38.0	35.0	40.0	29.0	41.0
48	35.67549477677402	38.0	35.0	40.0	28.0	41.0
49	35.656895710286754	38.0	35.0	40.0	28.0	41.0
50	35.55244621940001	38.0	35.0	40.0	28.0	41.0
51	34.50226813060952	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	15.0
10	27.0
11	23.0
12	16.0
13	22.0
14	37.0
15	53.0
16	111.0
17	206.0
18	378.0
19	666.0
20	1230.0
21	1996.0
22	3237.0
23	4746.0
24	7187.0
25	11037.0
26	14525.0
27	16324.0
28	17411.0
29	18241.0
30	21634.0
31	26930.0
32	35764.0
33	51506.0
34	79688.0
35	95060.0
36	120443.0
37	182514.0
38	273337.0
39	279782.0
40	108.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.74880107446508	20.35037179940471	22.70471905633111	17.1961080697991
2	35.47593566814342	23.958657140122614	23.664413169157854	16.900994022576104
3	29.292857385800513	23.790336933076105	30.86516428226223	16.05164139886115
4	25.96710953548211	23.663622190741282	31.948409223757512	18.420859050019104
5	21.676288919104262	35.38971902073708	25.771025986013917	17.162966074144734
6	23.922272132960305	31.735794225382975	28.802767158892532	15.539166482764186
7	73.79354039566323	4.820064274906131	15.525403458315834	5.8609918711148135
8	73.38468365213718	5.121743442986671	14.826415831591202	6.667157073284942
9	66.45080865678418	6.719282550937032	17.58289651550278	9.247012276776005
10	32.93752773368073	27.595813193045398	23.157633297660208	16.309025775613662
11	26.049133997280617	21.683328627011754	31.850723389310875	20.416813986396754
12	23.263782601164163	19.28223454566595	35.288631979099186	22.1653508740707
13	23.263149818430904	20.26051665128214	37.60762250080482	18.86871102948214
14	18.6329994613437	24.7334995969965	35.25074411294539	21.382756828714413
15	17.00018271601423	23.088501784051818	39.04372291393285	20.867592586001106
16	20.45296170003409	24.768539940850633	33.14571325292246	21.632785106192806
17	19.69306873523342	25.083032959279638	31.87350356770815	23.35039473777879
18	21.04121234843865	23.32104943852397	33.41353854477373	22.22419966826365
19	19.86138894227993	26.63469531906883	30.901944778632824	22.601970960018413
20	20.701249824996026	26.536613995413905	31.158854568335393	21.603281611254673
21	19.803963909236806	27.432238856498326	32.65388287349803	20.109914360766837
22	18.713679259834038	22.931888057570575	32.48991304774267	25.864519634852723
23	17.81496958292499	25.838891934155793	32.642413686457736	23.70372479646148
24	19.831410860291857	24.012048183241223	32.18562365088744	23.970917305579484
25	20.27396328436386	26.559235978127866	29.539009869037702	23.627790868470573
26	19.169994708354395	24.301071696656614	31.020275149751992	25.508658445237003
27	20.913785725528907	22.862440152595557	30.883356785843386	25.34041733603215
28	18.214255487610508	24.302732751331416	31.01853499723553	26.464476763822546
29	19.566591286423566	22.813794979976382	30.715669361530136	26.903944372069923
30	21.337671058969814	23.07608342291164	30.374836761829282	25.211408756289266
31	19.82421295670105	26.45506412066534	27.88436211941085	25.836360803222764
32	19.546104945434355	25.946069509601294	27.656085748388183	26.85173979657617
33	18.990996292684162	24.56676134678313	26.10798279147357	30.334259569059142
34	16.916497199540917	26.879186747631216	28.89879193866437	27.3055241141635
35	16.728798021288394	27.18284336175319	27.626186764241766	28.462171852716654
36	17.528477200442634	28.570456797945354	26.191114623055277	27.709951378556735
37	17.31554581070146	29.04797046802984	26.807049516039854	26.829434205228843
38	17.938283118068558	28.18200729756687	26.302009797058666	27.577699787305903
39	19.629315874857724	24.676232759636687	26.23991799135777	29.454533374147818
40	20.279500133279864	24.639135871899466	30.007269091648297	25.074094903172377
41	18.02307600432507	26.425481527885548	27.712877998698048	27.83856446909133
42	19.54222915119315	25.372689255428288	29.95055593918009	25.134525654198477
43	20.22231239376171	22.610909016125678	30.401730027992723	26.765048562119887
44	21.113824167079954	22.840767343981486	28.59078494325125	27.454623545687305
45	20.117349557882612	21.284991896426124	29.357796713801072	29.239861831890195
46	21.53011610772177	23.039540220066016	28.69827891006338	26.732064762148834
47	18.197328549495868	24.030794371713977	31.711194796627584	26.060682282162567
48	18.580478494483323	23.798879499975083	30.161668078563142	27.45897392697845
49	20.71461736023609	21.327309241712722	31.631859661445418	26.32621373660577
50	18.37695974789936	21.16191565480753	32.29414588964111	28.166978707652003
51	18.767782183527558	20.629666278296263	30.00410517798201	30.59844636019417
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	656.0
1	988.0
2	1320.0
3	13368.0
4	25416.0
5	16071.0
6	6726.0
7	6350.0
8	5974.0
9	5899.5
10	5825.0
11	5717.0
12	5609.0
13	5525.5
14	5442.0
15	5136.5
16	4831.0
17	4638.5
18	4446.0
19	4365.0
20	4284.0
21	4318.0
22	4352.0
23	4562.0
24	4772.0
25	5674.5
26	8042.0
27	9507.0
28	10815.5
29	12124.0
30	13842.5
31	15561.0
32	17659.0
33	19757.0
34	22806.5
35	25856.0
36	28074.5
37	30293.0
38	33450.0
39	36607.0
40	40125.0
41	43643.0
42	49404.0
43	55165.0
44	66219.5
45	77274.0
46	107517.0
47	137760.0
48	146348.0
49	154936.0
50	149189.5
51	143443.0
52	122080.0
53	100717.0
54	86080.5
55	71444.0
56	64013.5
57	56583.0
58	52173.0
59	47763.0
60	43040.5
61	38318.0
62	34507.0
63	30696.0
64	26580.0
65	22464.0
66	19079.5
67	15695.0
68	13081.0
69	10467.0
70	9254.0
71	8041.0
72	6965.0
73	5889.0
74	4790.0
75	2846.5
76	2002.0
77	1597.5
78	1193.0
79	929.5
80	666.0
81	479.5
82	293.0
83	205.0
84	117.0
85	76.0
86	35.0
87	23.5
88	12.0
89	9.5
90	7.0
91	6.5
92	6.0
93	4.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1264257.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.819542314701664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78960089325408	28.644785342606244
2	9.916874212151876	7.699370362164071
3	3.764841183788728	4.38448234926654
4	2.170191987206521	3.3698343871356022
5	1.5404403457703273	2.9899594592952448
6	1.2174133920427903	2.835565841213375
7	1.0090382930143693	2.7419283298977954
8	0.8579537714213181	2.664429818699819
9	0.7475117743512107	2.6116258459648094
>10	4.862924818191315	30.421977507817456
>50	0.0760091184120074	2.0143795170277623
>100	0.04000376626127593	2.9543425150927045
>500	0.002963241702312897	0.8386631611808052
>1k	0.003598222067094233	3.664827371230279
>5k	4.233202431875568E-4	0.9071070147385216
>10k+	2.116601215937784E-4	1.2567211766689936
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15295	1.209801488146793	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	5824	0.46066582981150195	No Hit
CTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGCT	5216	0.4125743420839275	RNA PCR Primer, Index 16 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTT	4872	0.3853646845538526	No Hit
GCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	4166	0.3295216083438731	RNA PCR Primer, Index 14 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTG	3897	0.30824428893808775	RNA PCR Primer, Index 16 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTC	3822	0.30231195081379814	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCG	3699	0.2925829162899632	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGC	3640	0.2879161436321887	No Hit
CCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	3455	0.2732830429256077	RNA PCR Primer, Index 16 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCT	3044	0.24077383000450067	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCC	2641	0.20889739981665123	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	2397	0.1895975264522957	RNA PCR Primer, Index 16 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCT	1716	0.1357318962837461	RNA PCR Primer, Index 14 (95% over 21bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTC	1687	0.13343805887568747	No Hit
TCTGTCTCTTATACACATCTGACGCTCACCAACTCGTATGCCGTCTTCTGC	1306	0.10330178120429628	RNA PCR Primer, Index 14 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4370155751560007	0.0
2	0.0	0.0	0.0	1.274819913989007	0.0
3	0.0	0.0	0.0	2.0904768571579986	0.0
4	0.0	0.0	0.0	3.0807027368644193	0.0
5	0.0	0.0	0.0	5.464157999520667	0.0
6	0.0	0.0	0.0	7.7555433744879405	0.0
7	0.0	0.0	0.0	9.627789286513739	0.0
8	0.0	0.0	0.0	12.349308724412836	0.0
9	0.0	0.0	0.0	13.62990278084282	0.0
10	0.0	0.0	0.0	15.900801814820879	0.0
11	0.0	0.0	0.0	18.10114557404072	0.0
12	0.0	0.0	0.0	20.043630369458107	0.0
13	0.0	0.0	0.0	20.851061137094753	0.0
14	0.0	0.0	0.0	21.2743136878024	0.0
15	1.581956833143894E-4	0.0	0.0	21.831004297385736	0.0
16	1.581956833143894E-4	0.0	0.0	22.651802600262446	0.0
17	1.581956833143894E-4	0.0	0.0	23.646853448309955	0.0
18	1.581956833143894E-4	0.0	0.0	24.72210950779786	0.0
19	1.581956833143894E-4	0.0	0.0	25.445459269752906	0.0
20	1.581956833143894E-4	0.0	0.0	26.1313957526041	0.0
21	1.581956833143894E-4	0.0	0.0	26.88646374906368	0.0
22	1.581956833143894E-4	0.0	0.0	27.68218803613506	0.0
23	1.581956833143894E-4	0.0	0.0	28.4615390699834	0.0
24	1.581956833143894E-4	0.0	0.0	29.080796072317575	0.0
25	1.581956833143894E-4	0.0	0.0	29.64112518261714	0.0
26	1.581956833143894E-4	0.0	0.0	30.151068967781075	0.0
27	1.581956833143894E-4	0.0	0.0	30.7351274305778	0.0
28	1.581956833143894E-4	0.0	0.0	31.29237172505274	0.0
29	1.581956833143894E-4	0.0	0.0	31.877774851157636	0.0
30	1.581956833143894E-4	0.0	0.0	32.54789176567739	0.0
31	2.372935249715841E-4	0.0	0.0	33.074366999747674	0.0
32	2.372935249715841E-4	0.0	0.0	33.61096675755009	0.0
33	2.372935249715841E-4	0.0	0.0	34.150967722543754	0.0
34	2.372935249715841E-4	0.0	0.0	34.69792929760326	0.0
35	2.372935249715841E-4	0.0	0.0	35.26371615897717	0.0
36	2.372935249715841E-4	0.0	0.0	35.75546744055995	0.0
37	2.372935249715841E-4	0.0	0.0	36.28004432643046	0.0
38	3.163913666287788E-4	0.0	0.0	36.84013614320506	0.0
39	3.163913666287788E-4	0.0	0.0	37.650414433141364	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATACG	25	3.891027E-5	45.000004	1
CGTTTTT	7075	0.0	43.5053	1
CGTTATT	905	0.0	42.513813	1
GCGTACG	65	0.0	41.53846	1
GCGTAAG	105	0.0	40.714287	1
CGAATAT	95	0.0	40.263157	14
CACGGGC	255	0.0	39.705883	4
CATAAGG	170	0.0	39.705883	2
CGTAAGG	210	0.0	39.642857	2
ACGGGAG	740	0.0	39.222973	5
TAATACG	110	0.0	38.863636	4
TACGGGT	125	0.0	37.800003	4
ACGACCA	280	0.0	37.76786	28
TTAAGCG	30	1.1399862E-4	37.500004	1
CGGTCTA	120	0.0	37.500004	31
CACAACG	565	0.0	37.433628	12
TATGGGA	905	0.0	37.29282	4
ACGGGTA	175	0.0	37.285713	5
AGCGACT	520	0.0	37.211536	19
GTTTTTT	8310	0.0	36.95848	2
>>END_MODULE