FastQCFastQC Report
Sat 18 Jun 2016
SRR3550032_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3550032_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1284690
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC324362.5248114331083764TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212811.6565085740528844No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC209711.6323782391082675No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG205931.6029547984338632No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT96190.7487409413944219Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC75650.5888580124387984TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT57620.4485128708093003No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT56730.44158512948649087No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC50340.3918455035845223No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT48090.37433155080213903No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG47020.366002693256739Illumina PCR Primer Index 5 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC45440.35370400641399874No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT43900.34171667873183414No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA39820.3099580443531124No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT37400.2911208151382824Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT32210.2507219640535849No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC31680.24659645517595682TruSeq Adapter, Index 23 (95% over 24bp)
GAATATGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC31290.24356070336034374TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCC23780.18510302096225548No Hit
CGTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT21720.16906802419260678Illumina PCR Primer Index 5 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC21520.16751122838972826No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG21190.1649425153149787No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC17090.13302820135596916No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATGCG253.891062E-545.0000041
CGTTTTT89600.043.618861
CGTTATT12450.043.1927721
TAATACG5700.042.631584
CCGATGA15000.041.418
TACGGCT26250.040.9714287
ACAACGA1800.040.013
ATATGTC4050.040.03
ATAATAC8450.039.9408263
CGATGAA15700.039.84076719
ACGGCTG26950.039.823758
CTCGAAT1300.039.8076943
GATGAAT16200.039.58333620
CGGTCTA800.039.37531
TAAGGGA8600.039.2441864
GAATCTG39900.039.0225561
GTTATTT14300.038.706292
ACGTGCG356.2488634E-638.5714261
GTACGAG700.038.5714261
CGTTTTC4450.038.4269641
Produced by FastQC (version 0.11.5)