##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3550032_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1284690
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.827524928192794	31.0	31.0	34.0	30.0	34.0
2	31.947219173497107	33.0	31.0	34.0	30.0	34.0
3	31.88259580132172	33.0	31.0	34.0	30.0	34.0
4	35.6613844585075	37.0	35.0	37.0	33.0	37.0
5	31.757379601304596	37.0	35.0	37.0	0.0	37.0
6	33.28822206135332	37.0	35.0	37.0	17.0	37.0
7	35.12817255524679	37.0	35.0	37.0	32.0	37.0
8	35.157140633148856	37.0	35.0	37.0	32.0	37.0
9	37.24463567086223	39.0	37.0	39.0	34.0	39.0
10	37.02713495084417	39.0	37.0	39.0	33.0	39.0
11	37.066220644669144	39.0	37.0	39.0	33.0	39.0
12	37.028017654064406	39.0	37.0	39.0	33.0	39.0
13	36.8973518903393	39.0	37.0	39.0	33.0	39.0
14	37.866735165681995	40.0	37.0	41.0	33.0	41.0
15	37.94177895056395	40.0	37.0	41.0	33.0	41.0
16	37.96414076547649	40.0	37.0	41.0	33.0	41.0
17	37.86607274906787	40.0	37.0	41.0	33.0	41.0
18	37.75276915053437	39.0	37.0	41.0	33.0	41.0
19	37.67130046937393	39.0	37.0	41.0	33.0	41.0
20	37.445989304812834	39.0	35.0	41.0	32.0	41.0
21	37.46678186955608	39.0	35.0	41.0	33.0	41.0
22	37.49841051148526	39.0	35.0	41.0	33.0	41.0
23	37.223771493512054	39.0	35.0	41.0	32.0	41.0
24	36.979416824292244	39.0	35.0	41.0	32.0	41.0
25	36.88447485385579	39.0	35.0	40.0	31.0	41.0
26	37.147650405934506	39.0	35.0	40.0	32.0	41.0
27	37.258903704395614	39.0	35.0	41.0	33.0	41.0
28	37.18202290046626	39.0	35.0	41.0	32.0	41.0
29	37.239301310043665	39.0	35.0	41.0	33.0	41.0
30	37.0321805260413	39.0	35.0	41.0	32.0	41.0
31	37.01311833983296	39.0	35.0	41.0	32.0	41.0
32	36.72722991538815	39.0	35.0	41.0	31.0	41.0
33	36.53903354116557	39.0	35.0	41.0	30.0	41.0
34	36.24122862324763	39.0	35.0	41.0	30.0	41.0
35	36.12317446232165	39.0	35.0	41.0	29.0	41.0
36	35.96964248184387	39.0	35.0	41.0	29.0	41.0
37	35.94388918727475	39.0	35.0	41.0	29.0	41.0
38	35.855936451595326	39.0	35.0	41.0	28.0	41.0
39	35.6829655403249	39.0	35.0	40.0	27.0	41.0
40	35.55390171948096	39.0	35.0	40.0	26.0	41.0
41	35.35013193844429	38.0	35.0	40.0	25.0	41.0
42	35.44712887934054	39.0	35.0	40.0	26.0	41.0
43	35.40390444387362	39.0	35.0	40.0	25.0	41.0
44	35.392008188745926	38.0	35.0	40.0	25.0	41.0
45	35.36086604550514	38.0	35.0	40.0	25.0	41.0
46	35.264957304875104	38.0	35.0	40.0	25.0	41.0
47	35.12153593473912	38.0	35.0	40.0	25.0	41.0
48	35.11307474955048	38.0	35.0	40.0	25.0	41.0
49	35.148185165292794	38.0	35.0	40.0	26.0	41.0
50	34.99845877215515	38.0	34.0	40.0	24.0	41.0
51	33.972128684741065	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	12.0
10	16.0
11	28.0
12	21.0
13	22.0
14	40.0
15	75.0
16	110.0
17	214.0
18	496.0
19	934.0
20	1870.0
21	3443.0
22	5655.0
23	8113.0
24	10660.0
25	14441.0
26	18091.0
27	20012.0
28	19953.0
29	20757.0
30	23605.0
31	29380.0
32	39291.0
33	55304.0
34	83985.0
35	99386.0
36	121195.0
37	173545.0
38	257908.0
39	276029.0
40	90.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39608777214737	16.642692011302337	19.759630728035557	18.20158948851474
2	34.10931820127813	31.262872755295053	20.23850111700099	14.389307926425833
3	25.71764394523192	31.068428959515526	29.493574325323618	13.720352769928931
4	23.124177817216605	20.357829515291627	40.25788322474683	16.26010944274494
5	25.97778452389292	31.59338050424616	25.416092598214355	17.01274237364656
6	21.902715830278122	34.828791381578434	29.305669071916178	13.96282371622726
7	66.01421354568028	4.555028839642248	23.90350979613759	5.527247818539881
8	63.86972732721513	10.877954993033338	18.22276191143389	7.029555768317648
9	58.0110376822424	6.008375561419486	19.408184075535733	16.572402680802373
10	35.80536938872413	21.342269341241856	25.895585705500938	16.95677556453308
11	28.876071270111858	19.157617791062435	31.25415469879893	20.712156240026776
12	22.721045544061212	17.0385073441842	36.13354194397092	24.10690516778367
13	23.24085966264235	18.257244938467647	40.91516241272213	17.58673298616787
14	18.504308432384466	26.39804155087998	34.387595451042664	20.71005456569289
15	15.740295324163805	21.323042913076307	42.227463434758576	20.709198328001307
16	18.694237520335648	23.76191921786579	32.26194646179234	25.281896800006226
17	18.572262569180115	25.03981505265862	33.61814912546996	22.769773252691312
18	18.993064474698176	23.431722828075255	33.05233169091376	24.522881006312808
19	19.011356825382	25.39024978788657	30.186192778024267	25.41220060870716
20	20.803929352606467	25.33809712849014	33.937914983381205	19.920058535522188
21	19.309950260374098	28.500260763296982	32.899298663490804	19.290490312838116
22	17.738676256528812	20.94326257696409	34.84902972701585	26.469031439491243
23	19.400477936311482	25.376705664401527	33.58514505444894	21.637671344838054
24	21.833983295581035	22.655660120340315	30.81615019965906	24.694206384419587
25	19.747487720773105	29.214907876608365	28.447174026418825	22.590430376199706
26	19.28877783745495	21.658688088176913	32.736302142929425	26.31623193143871
27	24.18871478722493	21.87196911317127	29.691910110610344	24.247405988993453
28	18.752150324202727	22.48262226685037	33.099424763950836	25.665802644996067
29	22.426032739415735	19.60402898753785	33.83890277031813	24.131035502728285
30	25.3059492951607	22.233924137340523	31.78432150946921	20.675805058029564
31	23.436081856323316	19.50781900691996	29.911573998396502	27.144525138360226
32	24.569507040609018	21.29992449540356	33.02578832247468	21.104780141512737
33	25.908195751504255	19.060629412543104	29.432781449221213	25.598393386731427
34	21.71745712973558	18.981077147016013	36.802419260677674	22.49904646257074
35	22.431403684935667	19.548996255906097	35.07375320116137	22.94584685799687
36	20.924113988588687	21.246215040204252	31.896255127696175	25.933415843510886
37	21.710062349671905	20.62123936513867	35.69148977574356	21.97720850944586
38	22.203955818135114	20.47233184659334	29.90090994714678	27.42280238812476
39	22.939386155414926	19.74686500245195	33.64009994629055	23.67364889584258
40	23.14869735111194	19.267216215585083	36.128715876982	21.45537055632098
41	19.249857942382988	22.58607134795165	31.80494905385735	26.359121655808014
42	20.179809915232468	20.716904467225554	36.35912165580801	22.74416396173396
43	23.454374207007138	19.240906366516437	30.507982470479263	26.79673695599717
44	23.49025835026349	19.30963890121352	30.9521363130405	26.24796643548249
45	20.96536907736497	17.79635554102546	31.217102958690425	30.021172422919147
46	25.727918797530926	20.50424616055235	30.285827709408498	23.482007332508232
47	19.643182401980244	19.771540215927576	37.409569623800294	23.175707758291882
48	20.335956534261186	21.0956728860659	31.65541881699087	26.91295176268205
49	20.383594485829263	17.96861499661397	37.2998933594875	24.347897158069262
50	20.29010889786641	18.023881247616156	34.8824229969876	26.803586857529833
51	19.95999034786602	17.81176781947396	32.136001681339465	30.092240151320553
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	655.0
1	1144.0
2	1633.0
3	18222.0
4	34811.0
5	21990.5
6	9170.0
7	8616.0
8	8062.0
9	7849.0
10	7636.0
11	7407.5
12	7179.0
13	6926.5
14	6674.0
15	6359.5
16	6045.0
17	5642.0
18	5239.0
19	5039.0
20	4839.0
21	4872.5
22	4906.0
23	5191.5
24	5477.0
25	6085.5
26	7492.5
27	8291.0
28	9299.0
29	10307.0
30	12202.5
31	14098.0
32	15412.0
33	16726.0
34	18940.0
35	21154.0
36	22860.5
37	24567.0
38	27346.0
39	30125.0
40	33222.5
41	36320.0
42	41056.0
43	45792.0
44	59489.5
45	73187.0
46	140565.5
47	207944.0
48	171437.0
49	134930.0
50	130328.5
51	125727.0
52	105820.0
53	85913.0
54	75613.0
55	65313.0
56	60934.0
57	56555.0
58	53695.0
59	50835.0
60	47157.5
61	43480.0
62	39198.0
63	34916.0
64	31428.5
65	27941.0
66	24169.0
67	20397.0
68	17294.5
69	14192.0
70	12160.5
71	10129.0
72	8338.5
73	6548.0
74	5482.5
75	3549.5
76	2682.0
77	2167.5
78	1653.0
79	1197.5
80	742.0
81	629.5
82	517.0
83	348.0
84	179.0
85	114.5
86	50.0
87	34.0
88	18.0
89	14.0
90	10.0
91	7.0
92	4.0
93	5.5
94	7.0
95	5.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1284690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.86273724774266
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.2156153586492	26.61575113286771
2	9.165276687159112	6.573838192688972
3	3.7128178694929646	3.9945543510704966
4	2.2531910004086595	3.232223872665367
5	1.6245976172113918	2.9131258739680477
6	1.3675402143489106	2.942624122974999
7	1.0795794169670652	2.710167107813275
8	0.91268662314984	2.6185152444441786
9	0.7714360167783726	2.4899226455851178
>10	4.782734124342205	26.34781630431064
>50	0.06867391748121347	1.6929082820705266
>100	0.03681644415302135	2.580978139112145
>500	0.002936280703872784	0.7491907946894167
>1k	0.004065619436131547	4.094426110840733
>5k	0.001129338732258763	2.7259723011874732
>10k+	9.034709858070104E-4	7.717985523710921
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	32436	2.5248114331083764	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21281	1.6565085740528844	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	20971	1.6323782391082675	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	20593	1.6029547984338632	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	9619	0.7487409413944219	Illumina PCR Primer Index 5 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	7565	0.5888580124387984	TruSeq Adapter, Index 23 (95% over 24bp)
CGTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	5762	0.4485128708093003	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	5673	0.44158512948649087	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	5034	0.3918455035845223	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTGTTCT	4809	0.37433155080213903	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTG	4702	0.366002693256739	Illumina PCR Primer Index 5 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	4544	0.35370400641399874	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTT	4390	0.34171667873183414	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTA	3982	0.3099580443531124	No Hit
CTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGCT	3740	0.2911208151382824	Illumina PCR Primer Index 5 (95% over 24bp)
CGTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCT	3221	0.2507219640535849	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCTGC	3168	0.24659645517595682	TruSeq Adapter, Index 23 (95% over 24bp)
GAATATGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTC	3129	0.24356070336034374	TruSeq Adapter, Index 23 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCC	2378	0.18510302096225548	No Hit
CGTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTCTTCT	2172	0.16906802419260678	Illumina PCR Primer Index 5 (95% over 21bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGC	2152	0.16751122838972826	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCG	2119	0.1649425153149787	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTGCTGTTCTCGTATGCCGTC	1709	0.13302820135596916	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.113591605757031E-4	0.0	0.0	0.3053654967346208	0.0
2	3.113591605757031E-4	0.0	0.0	1.310666386443422	0.0
3	3.113591605757031E-4	0.0	0.0	2.0422047342160368	0.0
4	3.113591605757031E-4	0.0	0.0	3.461457627910235	0.0
5	3.113591605757031E-4	0.0	0.0	7.600121430072624	0.0
6	3.113591605757031E-4	0.0	0.0	9.871175147311803	0.0
7	3.113591605757031E-4	0.0	0.0	11.779806801640863	0.0
8	3.113591605757031E-4	0.0	0.0	14.089391215001285	0.0
9	3.113591605757031E-4	0.0	0.0	15.088309241918283	0.0
10	3.113591605757031E-4	0.0	0.0	18.327378589387322	0.0
11	3.113591605757031E-4	0.0	0.0	19.89826339428189	0.0
12	3.113591605757031E-4	0.0	0.0	23.008897088013452	0.0
13	3.113591605757031E-4	0.0	0.0	23.70385073441842	0.0
14	3.113591605757031E-4	0.0	0.0	24.117802738403817	0.0
15	3.113591605757031E-4	0.0	0.0	24.85603530812881	0.0
16	3.113591605757031E-4	0.0	0.0	25.54522881006313	0.0
17	3.113591605757031E-4	0.0	0.0	26.268905339031207	0.0
18	3.113591605757031E-4	0.0	0.0	27.041387416419525	0.0
19	3.113591605757031E-4	0.0	0.0	28.00512185819147	0.0
20	3.113591605757031E-4	0.0	0.0	28.590321400493504	0.0
21	3.113591605757031E-4	0.0	0.0	29.13527777129113	0.0
22	3.113591605757031E-4	0.0	0.0	29.83902731398236	0.0
23	3.113591605757031E-4	0.0	0.0	30.393246619807112	0.0
24	3.113591605757031E-4	0.0	0.0	30.876320357440317	0.0
25	3.113591605757031E-4	0.0	0.0	31.29035020121586	0.0
26	3.113591605757031E-4	0.0	0.0	31.702511890027946	0.0
27	3.113591605757031E-4	0.0	0.0	32.18978897632892	0.0
28	3.113591605757031E-4	0.0	0.0	32.60093874786914	0.0
29	3.113591605757031E-4	0.0	0.0	33.03232686484677	0.0
30	3.113591605757031E-4	0.0	0.0	33.55050634783488	0.0
31	3.113591605757031E-4	0.0	0.0	33.95223750476769	0.0
32	3.113591605757031E-4	0.0	0.0	34.38105690867057	0.0
33	3.113591605757031E-4	0.0	0.0	34.77173481540294	0.0
34	3.113591605757031E-4	0.0	0.0	35.17681308331193	0.0
35	3.113591605757031E-4	0.0	0.0	35.596291712397544	0.0
36	3.113591605757031E-4	0.0	0.0	35.999034786602216	0.0
37	3.113591605757031E-4	0.0	0.0	36.41843557589769	0.0
38	3.113591605757031E-4	0.0	0.0	36.87916929375958	0.0
39	3.113591605757031E-4	0.0	0.0	37.505546085047754	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATATGCG	25	3.891062E-5	45.000004	1
CGTTTTT	8960	0.0	43.61886	1
CGTTATT	1245	0.0	43.192772	1
TAATACG	570	0.0	42.63158	4
CCGATGA	1500	0.0	41.4	18
TACGGCT	2625	0.0	40.971428	7
ACAACGA	180	0.0	40.0	13
ATATGTC	405	0.0	40.0	3
ATAATAC	845	0.0	39.940826	3
CGATGAA	1570	0.0	39.840767	19
ACGGCTG	2695	0.0	39.82375	8
CTCGAAT	130	0.0	39.80769	43
GATGAAT	1620	0.0	39.583336	20
CGGTCTA	80	0.0	39.375	31
TAAGGGA	860	0.0	39.244186	4
GAATCTG	3990	0.0	39.022556	1
GTTATTT	1430	0.0	38.70629	2
ACGTGCG	35	6.2488634E-6	38.571426	1
GTACGAG	70	0.0	38.571426	1
CGTTTTC	445	0.0	38.426964	1
>>END_MODULE